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All Journal HAYATI Journal of Biosciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Biosaintifika: Journal of Biology & Biology Education PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Darmabakti Cendekia: Journal of Community Service and Engagements Journal Pharmasci (Journal of Pharmacy and Science) Sewagati Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Yosephine Sri Wulan Manuhara
Department Of Biology, Faculty Of Science And Technology, Airlangga University, Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : HAYATI Journal of Biosciences

 1 
Gynura procumbens Adventitious Root Extract Altered Expression of Antioxidant Genes and Exert Hepatoprotective Effects Against Cadmium-Induced Oxidative Stress in Mice Sugiharto; Dwi Winarni; Anjar Tri Wibowo; Ufairanisa Islamatasya; Idqa Nurtri Bhakti; Nabilatun Nisa; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.479-486

Abstract

Exposure to cadmium (Cd) could increase of reactive oxygen species (ROS) and changes in expression of antioxidant genes. Gynura procumbens is a medicinal plant that is rich in phenolic and flavonoid compounds. The aimed of study to evaluate the hepatoprotective effect of G. procumbens adventitious root (GAR) extract against Cd toxicity, especially expression rate of hepatic antioxidant genes. Twenty-five male mice were treated as follows: P1 (control), P2 (Cd100mg/L), P3 (GAR100mg/L + Cd100mg/L), P4 (GAR200mg/L + Cd100mg/L), and P5 (GAR300mg/L + Cd100mg/L). The samples (blood and liver) were collected for analysis of malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and their relative gene expression were determined. The hematological assay showed Cd-treated administered with GAR extract increased the number of red blood cells (RBC), haemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), and mean corpuscular haemoglobin (MCH), but reduced the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, the GAR extract decreased the MDA production, but increased the activities of SOD and CAT. These enzymatic activities were positively correlated with their respective gene transcripts. Our study revealed that GAR extract administration showed marked hepatoprotective effects against Cd-induced oxidative stress.
Optimization of Biomass and Secondary Metabolite Production in Gynura procumbens (Lour.) Merr. Adventitious Roots Culture by Using the Method of Subculture and Fed-batch Cultivation in a Bioreactor Dannis Yuda Kusuma; Alfinda Novi Kristanti; Anjar Tri Wibowo; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.797-807

Abstract

The valuable extract of bioactive compounds from Gynura procumbens has been widely manufactured into various health products. The demand for these compounds is continuously increasing, but production through conventional farming methods is insufficient due to limited agricultural land and environmental stresses. An alternative to producing plant biomass is in vitro cultivation methods. This method requires less space and enables biomass propagation in a controlled condition that can facilitate stable and efficient production of plant secondary metabolites. This study evaluated the effect of inoculum subculture periods and culture methods on G. procumbens biomass and secondary metabolite production in a bioreactor. The 3-L airlift balloon type-bubble bioreactors was modified in this study to adopt the treatment of 1st-5th subculture periods and fed- and batch-cultivation strategies. We found the G. procumbens adventitious root culture was optimally derived from the 1st subculture produced biomass of 148.02±1.45 g FW and 8.59±0.12 g DW, and TPC (14.48±1.08 mg GAE/g DW) and TFC (116.89±0.44 mg KE/g DW and 33.97±0.13 mg QE/g DW). Additionally, the fed method after 28 days of culture using double distilled water replenishment improved adventitious root biomass (213.75±35.00 g FW and 11.21±0.18 g DW), while nutrient replenishment improved TFC (52.14±0.44 mg KE/g DW and 14.54±0.13 mg QE/g DW). These results can be used to optimize the cultivation of G. procumbens adventitious roots in a large-scale bioreactor.
Co-Authors Abdul Hakim Muhsyi Ahimsa Buena Merpati Alfiah Hayati Alfinda Novi Kristanti Anjar Tri Wibowo Arga Wal Yudha Arif Yachya Boon Chin Tan Boon Chin Tan Dannis Yuda Kusuma Dannis Yuda Kusuma Dewi, Firli Rahma Primula Djarot Sugiarso Djarot Sugiarso Dwi Winarni Hari Soepriandono Harmami Harmami Hendro Juwono Idqa Nurtri Bhakti Ira Nailas Sa'adah Ita Ulfin Junairiah Junairiah Kusuma, Dannis Yuda Kusuma, Dannis Yuda Lilis Sulistyorini Manikya Pramudya Muhammad Hilman Fu'adil Amin Nabilatun Nisa Ni'mah, Yatim Lailun Ni'matuzahroh Ni'matuzahroh Nindi Novia Erin Ni’matuzzahroh Ni’matuzahroh Nur Syamsiatul Fajar, Nur Syamsiatul Nurul Sofi Amalia Oktavia, Gebby Agnessya Esa Palupi Dasawulan Lestari Putri Gehasti R. Djarot Sugiarso Rachmah, Artifa Rafika Lailiyatul Kurnia Sari Rafika Lailiyatul Kurnia Sari S. Sugiharto Sofiana, Dewi Amelia Sugiharto Sugiharto Surahmaida, Surahmaida Syifa Fajrisani Ufairanisa Islamatasya Ufairanisa Islamatasya Wijayanti, Ely Tri Yohanes Kartjito Putro