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All Journal Jurnal Fitopatologi Indonesia Jurnal Agroteknos JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrokompleks
Botek, Muhammad
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Begomoviruses on two chili types in Southeast Sulawesi Indonesia: variation of symptom severity assessment and DNA-betasatellite identification Taufik, Muhammad; Firihu, Muhammad Zamrun; Hasan, Asmar; HS, Gusnawaty; Variani, Viska Inda; Syair, Syair; Botek, Muhammad
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.1241-9

Abstract

The association of viral satellite DNA with Begomoviruses influences symptom expression in infected plants. Normalized Difference Vegetation Index (NDVI) is an image processing method used to assess plant health based on the plant’s ability to absorb sunlight for photosynthesis. Therefore, this study aims to assess symptom severity based on symptom variation and NDVI, as well as to detect and identify the presence of beta-satellite DNA associated with chili plants. The study was conducted in North Kolaka Regency, Southeast Sulawesi Province, Indonesia. It involved observations and image recording of symptom variations in the sampled cayenne and big red chili plants, followed by the detection of beta-satellite DNA in the samples using the polymerase chain reaction (PCR). The results confirmed the presence of non-coding satellite DNA associated with the Pepper yellow leaf curl Indonesia virus (PepYLCIV) in all severe, mild, or asymptomatic plant samples. In the phylogenetic tree, the non-coding satellite DNA isolates from Southeast Sulawesi are included in the betasatellite group and exhibit different genetic characteristics from other isolates available in the GenBank database. The cayenne chili with severe symptoms had lower NDVI values than the mild and asymptomatic categories, indicating that this type of chili with severe symptoms had a lower ability to absorb light. Further studies are needed to characterize the alpha-satellite type in the Southeast Sulawesi region for potential use in cross-protection mechanisms against plant virus infections.
Identifikasi kutu daun yang berasosiasi dengan tanaman jeruk Syarni, Elisa; Taufik, Muhammad Taufik; Yusuf, Dewi Nurhayati; Nurulita, Sari; Miftakhurohmah, Miftakhurohmah; Botek, Muhammad; HS, Gusnawaty; Hikamwaty, Aisyah
Agrokompleks Vol 25 No 2 (2025): Vol 25 No 2 (2025): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i2.918

Abstract

Aphids are polyphagous insects commonly found on various plants, including citrus plants. The association between aphids and citrus plants plays a role as pests and vectors of citrus plant diseases, particularly Citrus tristeza virus (CTV). Various aphid species have been reported as CTV vectors, including Aphis gossypii, Toxoptera aurantii, and T. citricidus. So far, no information has been on aphid species associated with citrus plants in Southeast Sulawesi. Therefore, this study aimed to identify aphid species associated with citrus plants. The research method used was observation and collection of aphids found colonizing citrus plants. The collected aphids were then brought to the laboratory for detection using the polymerase chain reaction (PCR) technique. The study results showed that the aphid species associated with citrus plants was Aphis citricidus. The PCR technique successfully detected the genome of aphid vector samples from Konawe and South Konawe (700 bp). This information is a fundamental basis for controlling CTV in mainland Southeast Sulawesi.
Co-Authors Abdul Rahman Amelia, Devi ANDI KHAERUNI Asmar Hasan Asniah Asniah Ayu, Sukma AYUNI, RANI Eka Fatmawati Tihurua Firihu, Muhammad Zamrun GUSNAWATY HS, GUSNAWATY Hikamwaty, Aisyah Hisein, Waode Siti Anima HUTTNI, LITA OKTAFIANA La Ode Santiaji Bande M, Rahayu Mariadi Mariadi, Mariadi MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH MUHAMMAD TAUFIK NOOR AIDAWATI Nurul Amalia PADDA, PUTRI MEGAYANTI PURNAMA HIDAYAT PUTRI, NOVITA PRAMASARI RAHAYU M, RAHAYU Rahayu Mallarangeng, Rahayu RAHMAYANI, DITA Sari Nurulita Satrah, Vit Neru SEDYO HARTONO SUSLINGSIH, SRI ELSA Syair, Syair Syarni, Elisa Taufik, Muhammad Taufik Teguh Wijayanto TERRY PAKKI, TERRY Ulfa, Nur Isnaini Viska Inda Variani Widodo, Catur Joko Wulansari, Tri Yuni Indah Yusuf, Dewi Nurhayati Yuswana, Agung