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All Journal Jurnal Fitopatologi Indonesia Cropsaver : Journal of Plant Protection Jurnal Agroteknos JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrokompleks Jurnal Riset Perkebunan (JRP)
Botek, Muhammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Begomoviruses on two chili types in Southeast Sulawesi Indonesia: variation of symptom severity assessment and DNA-betasatellite identification Taufik, Muhammad; Firihu, Muhammad Zamrun; Hasan, Asmar; HS, Gusnawaty; Variani, Viska Inda; Syair, Syair; Botek, Muhammad
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.1241-9

Abstract

The association of viral satellite DNA with Begomoviruses influences symptom expression in infected plants. Normalized Difference Vegetation Index (NDVI) is an image processing method used to assess plant health based on the plant’s ability to absorb sunlight for photosynthesis. Therefore, this study aims to assess symptom severity based on symptom variation and NDVI, as well as to detect and identify the presence of beta-satellite DNA associated with chili plants. The study was conducted in North Kolaka Regency, Southeast Sulawesi Province, Indonesia. It involved observations and image recording of symptom variations in the sampled cayenne and big red chili plants, followed by the detection of beta-satellite DNA in the samples using the polymerase chain reaction (PCR). The results confirmed the presence of non-coding satellite DNA associated with the Pepper yellow leaf curl Indonesia virus (PepYLCIV) in all severe, mild, or asymptomatic plant samples. In the phylogenetic tree, the non-coding satellite DNA isolates from Southeast Sulawesi are included in the betasatellite group and exhibit different genetic characteristics from other isolates available in the GenBank database. The cayenne chili with severe symptoms had lower NDVI values than the mild and asymptomatic categories, indicating that this type of chili with severe symptoms had a lower ability to absorb light. Further studies are needed to characterize the alpha-satellite type in the Southeast Sulawesi region for potential use in cross-protection mechanisms against plant virus infections.
Identifikasi kutu daun yang berasosiasi dengan tanaman jeruk Syarni, Elisa; Taufik, Muhammad Taufik; Yusuf, Dewi Nurhayati; Nurulita, Sari; Miftakhurohmah, Miftakhurohmah; Botek, Muhammad; HS, Gusnawaty; Hikamwaty, Aisyah
Agrokompleks Vol 25 No 2 (2025): Vol 25 No 2 (2025): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i2.918

Abstract

Aphids are polyphagous insects commonly found on various plants, including citrus plants. The association between aphids and citrus plants plays a role as pests and vectors of citrus plant diseases, particularly Citrus tristeza virus (CTV). Various aphid species have been reported as CTV vectors, including Aphis gossypii, Toxoptera aurantii, and T. citricidus. So far, no information has been on aphid species associated with citrus plants in Southeast Sulawesi. Therefore, this study aimed to identify aphid species associated with citrus plants. The research method used was observation and collection of aphids found colonizing citrus plants. The collected aphids were then brought to the laboratory for detection using the polymerase chain reaction (PCR) technique. The study results showed that the aphid species associated with citrus plants was Aphis citricidus. The PCR technique successfully detected the genome of aphid vector samples from Konawe and South Konawe (700 bp). This information is a fundamental basis for controlling CTV in mainland Southeast Sulawesi.
Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara: Current Status of Citrus Tristeza Disease in Southeast Sulawesi Taufik, Muhammad; Yusuf, Dewi Nurhayati; Miftahkuhrohmah, Miftahkuhrohmah; Botek, Muhammad; Gusnawaty HS, Gusnawaty HS; Syarni, Elisa; Nurulita, Sari
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025 - IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.28-37

Abstract

Penyakit tristeza yang disebabkan oleh citrus tristeza virus (CTV, genus Closterovirus, famili Closteroviridae) merupakan salah satu penyakit penting pada tanaman jeruk di dunia, termasuk di Indonesia. Hingga kini, informasi dan studi terkait CTV di Indonesia masih terbatas. Penelitian ini bertujuan untuk mengetahui status terkini infeksi CTV di Provinsi Sulawesi Tenggara. Survei dan pengambilan sampel dilakukan secara purposive sampling di empat kabupaten. Deteksi dan identifikasi virus dilakukan dengan teknik double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription polymerase chain reaction (RT-PCR), dan analisis perunutan nukleotida. Hasil DAS-ELISA menunjukkan bahwa semua sampel dengan berbagai variasi gejala dari empat kabupaten terkonfirmasi positif terinfeksi CTV dengan nilai  titer virus yang beragam. RT-PCR dengan primer spesifik berhasil mengonfirmasi keberadaan CTV dari semua sampel dengan teramplifikasinya DNA berukuran 630 pb. Analisis sekuen  dan  filogeni menunjukkan bahwa isolat CTV asal Sulawesi Tenggara memiliki kemiripan tertinggi (97.9%) dengan isolat CTV asal India (MT498411.1) dan mengelompok satu grup dengan isolat asal India (MT498411.1), Jepang (U56902.1), dan Cina (MZ692538.1). Hasil penelitian ini mengonfirmasi penyebaran CTV pada empat kabupaten di Sulawesi Tenggara. Data molekuler diharapkan menjadi basis data untuk studi epidemiologi molekuler dan strategi pengendalian penyakit tristeza di Indonesia.
Inventarisasi Dan Peta Sebaran Hama Dan Penyakit Pada Empat Komoditi Unggulan Perkebunan Di Kabupaten Buton, Provinsi Sulawesi Tenggara luddin, Awa; Botek, Muhammad; Iswandi, Marsuki; Efendi, Siska
Jurnal Riset Perkebunan Vol. 4 No. 1 (2023): Jurnal Riset Perkebunan (JRP)
Publisher : Jurusan Budidaya Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrp.4.1.1-13.2023

Abstract

Information on the type of OPT that attacks plantation commodities in Buton Regency is incomplete, even though the data can be the basis for mitigating attacks and controls if the plants have been attacked. For this reason, research was conducted to identify the type and distribution of pests in plantation crops, especially in cocoa, cloves, cashew, pepper, and coconut commodities. They analyzed the damage caused by OPT in plantation crops, especially in cocoa, cloves, cashews, pepper, and coconut commodities, and determined alternative solutions for control and prevention. Observations were made in several villages in several sub-districts in Buton Regency. Direct observations were made on the specified land, and interviews with farmers and various related parties were conducted. To get OPT-type data and damage levels. OPT that attacks cashew consists of two types of pests and one disease. The level of damage OPT in the categorized category of guava plants. The level of damage to the pest on the categorized cashew was low. In coconut commodities, found OPT from three types of pest groups, namely Orycter Rhynocheros, Aspidiotus Destructors, and Pestalotia sp. The level of attack of the three pests was categorized as low. Cocoa was the commodity mainly attacked by OPT, consisting of 4 types of pests and four types of diseases. Fruit rot (Phytophthora palmivora) and stem cancer (Phytophthora palmivora) had a heavy attack rate in all cocoa-producing districts in Buton Regency.
Relationship Between whitefly (Bemisia tabaci) Population and Pepper Yellow Leaf Curl Disease on Chili Plant Yield in The Field Taufik, Muhammad; Hasan, Asmar; Mallarangeng, Rahayu; HS, Gusnawaty; Khaeruni, Andi; Botek, Muhammad; Syair, Syair
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.44927

Abstract

Whitefly vector insects can spread the Pepper yellow leaf curl Indonesia virus (PYLCIV)  that causes Begomovirus disease. One whitefly can transmit the virus, which belongs to the Begomovirus genus. It is suspected that the more whitefly, the higher the incidence and severity of Begomovirus disease. The increased severity of Begomovirus disease can affect chili yields. This study aimed to assess the relationship between the whitefly population level, the Begomovirus disease's intensity, and the production of chili peppers. The research method used was a survey of the farmers' chili plantations. Observations of the whitefly population and disease intensity were carried out on a scheduled basis. The results showed that the whitefly population affected the incidence and severity of the disease Begomovirus. Each addition of one whitefly/leaf will increase the incidence of disease by 25.981%, the severity by 15.269%, and reduce the yield of chili plants by 40.044 kg/ha. Meanwhile, every 1% increase in the severity of Begomovirus disease will reduce the production of chili plants by 2.867 kg/ha.
Metode Infrared Thermography (IRT) untuk deteksi cepat lubang aktif tikus sawah Hasan, Asmar; Taufik, Muhammad; Khaeruni, Andi; Mallarangeng, Rahayu; Syair, Syair; Bande, La Ode Santiaji; HS, Gusnawaty; Botek, Muhammad
Agrokompleks Vol 23 No 2 (2023): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v23i2.559

Abstract

Tikus sawah (Rattus-rattus argentiventer) dapat menyebabkan kerusakan yang parah pada tanaman padi sawah. Pengendalian dengan fumigasi (pengasapan) berbahan aktif sulfur dapat membunuh tikus dalam lubang pematang. Fumigasi akan efektif bila lubang aktif tikus diketahui, namun mengenali lubang aktif tikus tidaklah mudah. Tujuan penelitian ini adalah mengevaluasi potensi metode Infrared Thermography (IRT) dalam mendeteksi lubang aktif tikus sawah melalui visualisasi citra termal. Beberapa lubang tikus yang ditemukan di areal persawahan milik petani di Desa Lebo Jaya, Kecamatan Konda, Kabupaten Konawe Selatan, Provinsi Sulawesi Tenggara direkam citra termalnya menggunakan FLIR C2 Compact Thermal Imager. Selanjutnya, citra termal dan RGB (red green blue) diolah menggunakan aplikasi FLIR Tools versi 6.4.18039.1003 (FLIR® Systems, USA) dan dilanjutkan dengan analisis suhu rata-rata lubang tikus menggunakan Microsoft Excel. Hasil pengolahan citra termal menunjukkan bahwa area tanah galian lubang tikus yaitu area sisi dalam lubang berwarna lebih gelap yang menandakan bahwa suhu tanah di area tersebut lebih rendah sampai berkisar pada suhu 28 °C, sebaliknya area sisi luar lubang berwarna lebih terang yang menandakan bahwa suhu tanah yang lebih tinggi sampai berkisar pada suhu 32 °C. Metode IRT ini sangat potensial untuk dimanfaatkan sebagai metode deteksi cepat lubang aktif tikus sawah. Implementasinya bersama teknologi drone (UAV) akan mengefisienkan waktu petani saat menandai lubang aktif tikus sawah pada areal persawahan yang luas. Selain itu, pengendalian tikus dengan teknik fumigasi juga akan menjadi lebih efektif dan ekonomis.
Co-Authors Abdul Rahman Amelia, Devi ANDI KHAERUNI Asmar Hasan Asniah Asniah Ayu, Sukma AYUNI, RANI Eka Fatmawati Tihurua Firihu, Muhammad Zamrun GUSNAWATY HS, GUSNAWATY Hikamwaty, Aisyah Hisein, Waode Siti Anima HUTTNI, LITA OKTAFIANA La Ode Santiaji Bande luddin, Awa M, Rahayu Mariadi Mariadi, Mariadi Marsuki Iswandi, Marsuki Miftahkuhrohmah, Miftahkuhrohmah MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH MUHAMMAD TAUFIK NOOR AIDAWATI Nurul Amalia PADDA, PUTRI MEGAYANTI PURNAMA HIDAYAT PUTRI, NOVITA PRAMASARI RAHAYU M, RAHAYU Rahayu Mallarangeng, Rahayu RAHMAYANI, DITA Sari Nurulita Satrah, Vit Neru SEDYO HARTONO Siska Efendi SUSLINGSIH, SRI ELSA Syair, Syair Syarni, Elisa Taufik, Muhammad Taufik Teguh Wijayanto TERRY PAKKI, TERRY Ulfa, Nur Isnaini Viska Inda Variani Widodo, Catur Joko Wulansari, Tri Yuni Indah Yusuf, Dewi Nurhayati Yuswana, Agung