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All Journal JURNAL ILMIAH KESEHATAN KEPERAWATAN Jurnal Surya Masyarakat Jurnal Labora Medika PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kesehatan Jaringan Laboratorium Medis Bali Medika Jurnal Borneo Journal of Medical Laboratory Technology Jurnal Surya Masyarakat JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Prosiding University Research Colloquium Prosiding Seminar Kesehatan Masyarakat Prosiding Seminar Nasional Unimus Jurnal Ilmiah Kesehatan Keperawatan
Gela Setya Ayu Putri
Program Studi DIV Teknologi Laboratorium Medik, Fakultas Ilmu Keperawatan Dan Kesehatan Universitas Muhammadiyah Semarang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jaringan Laboratorium Medis

 1 
Efektifitas Minyak Kenanga (Cananga Odorata) sebagai Agen Deparafinisasi pada Pewarnaan Hematoxylin-Eosin Putri, Gela Setya Ayu; Diyanah, Dina; Iswara, Arya
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10824

Abstract

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p0.05). The Kruskal-Wallis test was then used.
Penggunaan Minyak Kelapa Mandar sebagai Agen Alternatif Deparafinisasi pada Pewarnaan Rutin Hematoksilin-Eosin Putri, Gela Setya Ayu; Zalzabillah, Nurul Fhatiah
Jaringan Laboratorium Medis Vol 7, No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12688

Abstract

The deparaffinization technique is critical in hematoxylin-eosin staining tissue slides. Xylol is an organic solvent that is frequently used as a deparaffinization agent, however, it has several hazardous effects. Alternative deparaffinization agents that are safer for the environment and humans are required, one of which is traditional mandar coconut oil (MTM). Lauric acid in MTM is non-polar, hence it can dissolve paraffin. The study aimed to evaluate the quality of staining results in mouse liver slide during the deparaffinization process using xylol and MTM with Hematoxylin-eosin (HE) staining. This study is an experimental design that uses 40 samples of mouse liver tissue slides, which were divided into two treatment groups. The Man-Whitney test was used to analyze data on the quality of tissue staining results based on nucleus, cytoplasm, and color homogeneity. The results of the staining quality of the mouse liver slide in the xylol and MTM groups obtained 100% good (score 3). The conclusion in this study is traditional mandar coconut oil (MTM) is effective for use as an alternative deparaffinization agent in HE staining. 
Efektifitas Minyak Kenanga (Cananga Odorata) sebagai Agen Deparafinisasi pada Pewarnaan Hematoxylin-Eosin Putri, Gela Setya Ayu; Diyanah, Dina; Iswara, Arya
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10824

Abstract

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p<0.05). The Kruskal-Wallis test was then used.
Penggunaan Minyak Kelapa Mandar sebagai Agen Alternatif Deparafinisasi pada Pewarnaan Rutin Hematoksilin-Eosin Putri, Gela Setya Ayu; Zalzabillah, Nurul Fhatiah
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12688

Abstract

The deparaffinization technique is critical in hematoxylin-eosin staining tissue slides. Xylol is an organic solvent that is frequently used as a deparaffinization agent, however, it has several hazardous effects. Alternative deparaffinization agents that are safer for the environment and humans are required, one of which is traditional mandar coconut oil (MTM). Lauric acid in MTM is non-polar, hence it can dissolve paraffin. The study aimed to evaluate the quality of staining results in mouse liver slide during the deparaffinization process using xylol and MTM with Hematoxylin-eosin (HE) staining. This study is an experimental design that uses 40 samples of mouse liver tissue slides, which were divided into two treatment groups. The Man-Whitney test was used to analyze data on the quality of tissue staining results based on nucleus, cytoplasm, and color homogeneity. The results of the staining quality of the mouse liver slide in the xylol and MTM groups obtained 100% good (score 3). The conclusion in this study is traditional mandar coconut oil (MTM) is effective for use as an alternative deparaffinization agent in HE staining. 
Co-Authors Afiana Rohmani Agita, Serly Ali Ali Ana Hidayati Mukaromah Andri Sukeksi Ardianto, Chandra Eko Arum Kartikadewi Arya Iswara Arya Iswara Bano, Siti Maryam Bintang Satria Purbaya Budi Santosa Budi Santosa Devi Kemala Dewi Dewi, Devi Kemala Diyanah, Dina Djafar, Hawin Pratiwi Eka Sakti Wahyuningtyas ERMA WIDYA LESTARI, ERMA Halmi, Meutya Fajri Handayani, Sri Puji Heni Lutfiyati Heni Lutfiyati Heni Lutfiyati, Heni Heni Setyowati Esti Rahayu Hidayani, Al Hikmayanti, Hikmayanti Isabella Meliawati Sikumbang Isabella Meliawati Sikumbang Kadarwati, Ari Kartikadewi, Arum Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Maolida, Nurul Mudyawati Khamaruddin Muhamad Jumardi N Nasruddin Nasruddin Nasruddin Nasruddin Nasruddin Nasruddin Nasruddin Nasruddin, N Nia Salsabila Nia Salsabila Nia Salsabila Nuria Wijayanti Nurul Qomariyah Pratiwi R. H. Sya’diah Putri Kurniasiwi Putri, Aisya Putri, isya Ragil Saptaningtyas, Ragil Rauf, Mohamad Jois Romantir, Maya Salsabila, Nia Sari, Sri Mayang Sikumbang, Isabella Meliawati Sri Darmawati Sri Darmawati Tulus Ariyadi Tulus Ariyadi Ulya, Iha Himatul Wijanarko Wijanarko Wulandari, Try Zalzabillah, Nurul Fhatiah