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SENSITIVITAS DAN SPESIFISITAS METODE RAPID TEST ANTI-HBs TERHADAP ELISA: The comparison of sensitivity and specificity rapid tests anti-hbs and elisa Putri, Gela Setya Ayu; Santosa, Budi
Bali Medika Jurnal Vol 9 No 3 (2022): Bali Medika Jurnal Vol 9 No 3 Desember 2022
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v9i3.298

Hepatitis B merupakan penyakit inflamasi dan nekrosis sel hati yang disebabkan oleh infeksi virus hepatitis B (VHB). Kehadiran anti-HBs merupakan faktor penting dalam diagnosis dan prognosis infeksi VHB. Rapid test adalah salah satu metode pemeriksaan selain ELISA untuk mendeteksi adanya anti-HBs, lebih praktis dalam penggunaan dan ekonomis. Tujuan penelitian ini untuk mengetahui nilai sensitivitas dan spesifisitas rapid test anti-HBs Intec® terhadap metode ELISA sebagai gold standar. Penelitian ini merupakan studi observasional menggunakan metode uji diagnostik. Populasi penelitian ini adalah mahasiswa D4 Analis Kesehatan FIKKES Universitas Muhammadiyah Semarang semester tujuh post vaksinasi hepatitis B sejumlah 32 orang untuk didapatkan serum darah. Analisis statistik dilakukan uji Mc Nemar. Hasil pemeriksaan dengan rapid test yaitu positif 26 sampel (81,2%), dan negatif 6 sampel (18,8%). Hasil pemeriksaan dengan ELISA yaitu reaktif 27 sampel (84,4%), dan nonreaktif 5 sampel (15,6%). Kesimpulan akhir yaitu Rapid test anti-HBs Intec® memiliki nilai sensitivitas dan spesifisitas yang sangat baik dengan persentase sensitivitas 96,3% dan persentase spesifisitas 100%. Tidak terdapat perbedaan yang bermakna pada sensitivitas dan spesifisitas metode rapid test dan ELISA (p= 1,000) sehingga Rapid test anti-HBs Intec® dapat digunakan sebagai skrining untuk pemeriksaan anti-HBs.

Penggunaan Minyak Kelapa Mandar sebagai Agen Alternatif Deparafinisasi pada Pewarnaan Rutin Hematoksilin-Eosin Putri, Gela Setya Ayu; Zalzabillah, Nurul Fhatiah
Jaringan Laboratorium Medis Vol 7, No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12688

The deparaffinization technique is critical in hematoxylin-eosin staining tissue slides. Xylol is an organic solvent that is frequently used as a deparaffinization agent, however, it has several hazardous effects. Alternative deparaffinization agents that are safer for the environment and humans are required, one of which is traditional mandar coconut oil (MTM). Lauric acid in MTM is non-polar, hence it can dissolve paraffin. The study aimed to evaluate the quality of staining results in mouse liver slide during the deparaffinization process using xylol and MTM with Hematoxylin-eosin (HE) staining. This study is an experimental design that uses 40 samples of mouse liver tissue slides, which were divided into two treatment groups. The Man-Whitney test was used to analyze data on the quality of tissue staining results based on nucleus, cytoplasm, and color homogeneity. The results of the staining quality of the mouse liver slide in the xylol and MTM groups obtained 100% good (score 3). The conclusion in this study is traditional mandar coconut oil (MTM) is effective for use as an alternative deparaffinization agent in HE staining.

Efektifitas Minyak Kenanga (Cananga Odorata) sebagai Agen Deparafinisasi pada Pewarnaan Hematoxylin-Eosin Putri, Gela Setya Ayu; Diyanah, Dina; Iswara, Arya
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10824

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses aÂ xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylangÂ oil, which contains Î²-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimedÂ to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) â‰¥ 15, resulting inÂ a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p<0.05). The Kruskal-Wallis test was then used.

Penggunaan Minyak Kelapa Mandar sebagai Agen Alternatif Deparafinisasi pada Pewarnaan Rutin Hematoksilin-Eosin Putri, Gela Setya Ayu; Zalzabillah, Nurul Fhatiah
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12688

The deparaffinization technique is critical in hematoxylin-eosin staining tissue slides. Xylol is an organic solvent that is frequently used as a deparaffinization agent, however, it has several hazardous effects. Alternative deparaffinization agents that are safer for the environment and humans are required, one of which is traditional mandar coconut oil (MTM). Lauric acid in MTM is non-polar, hence it can dissolve paraffin. The study aimed to evaluate the quality of staining results in mouse liver slide during the deparaffinization process using xylol and MTM with Hematoxylin-eosin (HE) staining. This study is an experimental design that uses 40 samples of mouse liver tissue slides, which were divided into two treatment groups. The Man-Whitney test was used to analyze data on the quality of tissue staining results based on nucleus, cytoplasm, and color homogeneity. The results of the staining quality of the mouse liver slide in the xylol and MTM groups obtained 100% good (score 3). The conclusion in this study is traditional mandar coconut oil (MTM) is effective for use as an alternative deparaffinization agent in HE staining.Â

Peningkatan Kewaspadaan Dini terhadap Anemia pada Lansia melalui Pemeriksaan Hemoglobin dan Golongan Darah Putri, Gela Setya Ayu; Halmi, Meutya Fajri; Ardianto, Chandra Eko
Jurnal Surya Masyarakat Vol 7, No 2 (2025): Mei 2025
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.7.2.2025.173-179

Anemia is still a worldwide health issue. The high prevalence of anemia necessitates special attention because anemia can trigger a variety of health problems in the elderly. The goal of this community service is to perform hemoglobin and blood type tests on elderly residents of Tambak Bulusan village in Demak to early detection of anemia and raise awareness about anemia in the elderly. Community service activities were conducted in Tambak Bulusan Village, Karangtengah, Demak Regency, Central Java. There are three steps involved: preparation, implementation, and evaluation. The hemoglobin test is performed using a POCT device, and the blood group test is performed manually. The community service was attended by 32 elderly people. The percentage of correct answers on the pre-test and post-test was 39.7% and 71.3%, respectively. There was a difference in the level of residents knowledge before and after education (p<0.05) with an increase in knowledge of 31.6%. The hemoglobin (Hb) level test showed an average of 11.9 ± 1.3 g/dL, with 21 residents (66%) having Hb levels below normal, 10 residents (31%) having normal Hb levels, and 1 resident (3%) having Hb levels above normal. Blood type test found all blood types, namely A, B, O, and AB rhesus positive with a dominance of blood type O. The conclusion after the community service activities, the knowledge of elderly residents increased by 31.6%. A total of 21 residents (66%) had hemoglobin levels below normal or showed signs of anemia. All types of blood types were found with a dominance of blood type O. All residents have positive rhesus.

Peningkatan Kewaspadaan Dini terhadap Anemia pada Lansia melalui Pemeriksaan Hemoglobin dan Golongan Darah Putri, Gela Setya Ayu; Halmi, Meutya Fajri; Ardianto, Chandra Eko
Jurnal Surya Masyarakat Vol 7, No 2 (2025): Mei 2025
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.7.2.2025.173-179

Anemia is still a worldwide health issue. The high prevalence of anemia necessitates special attention because anemia can trigger a variety of health problems in the elderly. The goal of this community service is to perform hemoglobin and blood type tests on elderly residents of Tambak Bulusan village in Demak to early detection of anemia and raise awareness about anemia in the elderly. Community service activities were conducted in Tambak Bulusan Village, Karangtengah, Demak Regency, Central Java. There are three steps involved: preparation, implementation, and evaluation. The hemoglobin test is performed using a POCT device, and the blood group test is performed manually. The community service was attended by 32 elderly people. The percentage of correct answers on the pre-test and post-test was 39.7% and 71.3%, respectively. There was a difference in the level of residents knowledge before and after education (p<0.05) with an increase in knowledge of 31.6%. The hemoglobin (Hb) level test showed an average of 11.9 ± 1.3 g/dL, with 21 residents (66%) having Hb levels below normal, 10 residents (31%) having normal Hb levels, and 1 resident (3%) having Hb levels above normal. Blood type test found all blood types, namely A, B, O, and AB rhesus positive with a dominance of blood type O. The conclusion after the community service activities, the knowledge of elderly residents increased by 31.6%. A total of 21 residents (66%) had hemoglobin levels below normal or showed signs of anemia. All types of blood types were found with a dominance of blood type O. All residents have positive rhesus.

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