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Penggunaan Minyak Kelapa Mandar sebagai Agen Alternatif Deparafinisasi pada Pewarnaan Rutin Hematoksilin-Eosin Putri, Gela Setya Ayu; Zalzabillah, Nurul Fhatiah
Jaringan Laboratorium Medis Vol 7, No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12688

The deparaffinization technique is critical in hematoxylin-eosin staining tissue slides. Xylol is an organic solvent that is frequently used as a deparaffinization agent, however, it has several hazardous effects. Alternative deparaffinization agents that are safer for the environment and humans are required, one of which is traditional mandar coconut oil (MTM). Lauric acid in MTM is non-polar, hence it can dissolve paraffin. The study aimed to evaluate the quality of staining results in mouse liver slide during the deparaffinization process using xylol and MTM with Hematoxylin-eosin (HE) staining. This study is an experimental design that uses 40 samples of mouse liver tissue slides, which were divided into two treatment groups. The Man-Whitney test was used to analyze data on the quality of tissue staining results based on nucleus, cytoplasm, and color homogeneity. The results of the staining quality of the mouse liver slide in the xylol and MTM groups obtained 100% good (score 3). The conclusion in this study is traditional mandar coconut oil (MTM) is effective for use as an alternative deparaffinization agent in HE staining.

Efektifitas Minyak Kenanga (Cananga Odorata) sebagai Agen Deparafinisasi pada Pewarnaan Hematoxylin-Eosin Putri, Gela Setya Ayu; Diyanah, Dina; Iswara, Arya
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10824

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses aÂ xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylangÂ oil, which contains Î²-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimedÂ to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) â‰¥ 15, resulting inÂ a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p<0.05). The Kruskal-Wallis test was then used.

Penggunaan Minyak Kelapa Mandar sebagai Agen Alternatif Deparafinisasi pada Pewarnaan Rutin Hematoksilin-Eosin Putri, Gela Setya Ayu; Zalzabillah, Nurul Fhatiah
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12688

The deparaffinization technique is critical in hematoxylin-eosin staining tissue slides. Xylol is an organic solvent that is frequently used as a deparaffinization agent, however, it has several hazardous effects. Alternative deparaffinization agents that are safer for the environment and humans are required, one of which is traditional mandar coconut oil (MTM). Lauric acid in MTM is non-polar, hence it can dissolve paraffin. The study aimed to evaluate the quality of staining results in mouse liver slide during the deparaffinization process using xylol and MTM with Hematoxylin-eosin (HE) staining. This study is an experimental design that uses 40 samples of mouse liver tissue slides, which were divided into two treatment groups. The Man-Whitney test was used to analyze data on the quality of tissue staining results based on nucleus, cytoplasm, and color homogeneity. The results of the staining quality of the mouse liver slide in the xylol and MTM groups obtained 100% good (score 3). The conclusion in this study is traditional mandar coconut oil (MTM) is effective for use as an alternative deparaffinization agent in HE staining.Â

Peningkatan Kewaspadaan Dini terhadap Anemia pada Lansia melalui Pemeriksaan Hemoglobin dan Golongan Darah Putri, Gela Setya Ayu; Halmi, Meutya Fajri; Ardianto, Chandra Eko
Jurnal Surya Masyarakat Vol 7, No 2 (2025): Mei 2025
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.7.2.2025.173-179

Anemia is still a worldwide health issue. The high prevalence of anemia necessitates special attention because anemia can trigger a variety of health problems in the elderly. The goal of this community service is to perform hemoglobin and blood type tests on elderly residents of Tambak Bulusan village in Demak to early detection of anemia and raise awareness about anemia in the elderly. Community service activities were conducted in Tambak Bulusan Village, Karangtengah, Demak Regency, Central Java. There are three steps involved: preparation, implementation, and evaluation. The hemoglobin test is performed using a POCT device, and the blood group test is performed manually. The community service was attended by 32 elderly people. The percentage of correct answers on the pre-test and post-test was 39.7% and 71.3%, respectively. There was a difference in the level of residents knowledge before and after education (p<0.05) with an increase in knowledge of 31.6%. The hemoglobin (Hb) level test showed an average of 11.9 ± 1.3 g/dL, with 21 residents (66%) having Hb levels below normal, 10 residents (31%) having normal Hb levels, and 1 resident (3%) having Hb levels above normal. Blood type test found all blood types, namely A, B, O, and AB rhesus positive with a dominance of blood type O. The conclusion after the community service activities, the knowledge of elderly residents increased by 31.6%. A total of 21 residents (66%) had hemoglobin levels below normal or showed signs of anemia. All types of blood types were found with a dominance of blood type O. All residents have positive rhesus.

Peningkatan Kewaspadaan Dini terhadap Anemia pada Lansia melalui Pemeriksaan Hemoglobin dan Golongan Darah Putri, Gela Setya Ayu; Halmi, Meutya Fajri; Ardianto, Chandra Eko
Jurnal Surya Masyarakat Vol 7, No 2 (2025): Mei 2025
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.7.2.2025.173-179

Anemia is still a worldwide health issue. The high prevalence of anemia necessitates special attention because anemia can trigger a variety of health problems in the elderly. The goal of this community service is to perform hemoglobin and blood type tests on elderly residents of Tambak Bulusan village in Demak to early detection of anemia and raise awareness about anemia in the elderly. Community service activities were conducted in Tambak Bulusan Village, Karangtengah, Demak Regency, Central Java. There are three steps involved: preparation, implementation, and evaluation. The hemoglobin test is performed using a POCT device, and the blood group test is performed manually. The community service was attended by 32 elderly people. The percentage of correct answers on the pre-test and post-test was 39.7% and 71.3%, respectively. There was a difference in the level of residents knowledge before and after education (p<0.05) with an increase in knowledge of 31.6%. The hemoglobin (Hb) level test showed an average of 11.9 ± 1.3 g/dL, with 21 residents (66%) having Hb levels below normal, 10 residents (31%) having normal Hb levels, and 1 resident (3%) having Hb levels above normal. Blood type test found all blood types, namely A, B, O, and AB rhesus positive with a dominance of blood type O. The conclusion after the community service activities, the knowledge of elderly residents increased by 31.6%. A total of 21 residents (66%) had hemoglobin levels below normal or showed signs of anemia. All types of blood types were found with a dominance of blood type O. All residents have positive rhesus.

Efek Perlakuan Kombinatif Plasma Medis dan Ekstrak Daun Binahong Terhadap Penyembuhan Luka Fase Proliferasi Pada Mencit Diabetik Wahyuningtyas, Eka Sakti; Nasruddin, N; Rahayu, Heni Setyowati Esti; Lutfiyati, Heni; Sikumbang, Isabella Meliawati; Nurani, Laela Hayu; Kartikadewi, Arum; Salsabila, Nia; Putri, Gela Setya Ayu; Kurniasiwi, Putri; Dewi, Devi Kemala
Prosiding University Research Colloquium Proceeding of The 12th University Research Colloquium 2020: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Plasma medicine is a relatively new and multidisciplinary studyinvolving the study of plasma science, biomedicine, pharmacy, andother health sciences to utilizing plasma for medical therapy. Plasmais the fourth substance, after solids, liquids, and gases. Plasma canproduce biological molecules of Reactive Oxygen and NitrogenSpecies (RON) which, if controlled in the right dose, can bebeneficial for health. This animal study examines the effect ofcombinative treatment of medicine plasma and binahong leaf extracton proliferation phase wound healing in a diabetic test model. Thestudy used Balb mice 7-8 years old which were induced by STZ withacute wounds which were divided into 4 groups, namely the woundgroup of diabetic mice without treatment (C), the group of diabeticmice with the treatment of binahong leaf extract (B), the group ofcombined treatment wounds plasma jet (P) and the wound group ofdiabetic mice treated with a combination of plasma and, binahongleaf extract (PB). Binahong leaf extract concentration 1% in DMSOsolvent. The jet type medicine plasma treatment was carried outevery day from day 0 to day 6. Macroscopic observation of thewound was carried out every day from day 0 to day 7. On day 7 itappears that the size of the wound area for P is much smaller thanfor C, B, and PB. The size of area B and PB is relatively the same,but smaller than C. The results of this study indicate that thebinahong leaf extract tends to inhibit the performance of medicineplasma in accelerating the healing of the proliferation phase.

Pengaruh Penundaan Sampel Terhadap Nilai Agregasi Trombosit Darah Sitrat Pada Suhu Ruang (25ºC) Putri, Gela Setya Ayu; Ulya, Iha Himatul; Sukeksi, Andri
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 1 (2025): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i1.10651

Pemeriksaan agregasi trombosit merupakan pemeriksaan penting untuk mengevaluasi fungsi trombosit. Pemeriksaan ini bersifat sangat sensitif dan dipengaruhi oleh berbagai faktor, seperti konsentrasi sodium sitrat, jumlah trombosit, suhu penyimpanan, dan waktu penundaan pemeriksaan. Metode pemeriksaan dapat dilakukan secara otomatis maupun manual, salah satunya menggunakan metode Velaskar. Permasalahan yang sering terjadi di laboratorium adalah penundaan pemeriksaan, yang berpotensi menyebabkan kerusakan fungsi trombosit sehingga meningkatkan persentase agregasi. Penelitian ini bertujuan mengetahui perbedaan persentase agregasi trombosit berdasarkan variasi waktu penundaan sampel darah sitrat pada suhu ruang (25°C). Desain penelitian yang digunakan adalah analitik eksperimental. Sampel darah vena diperoleh dari enam responden, kemudian dibagi ke dalam empat kelompok perlakuan yaitu kelompok segera diperiksa (kontrol), ditunda 2 jam, 3 jam, dan 4 jam. Agregasi trombosit diperiksa menggunakan metode Velaskar dengan pewarnaan Giemsa. Data dianalisis dengan One-Way Anova dan Post-Hoc. Hasil penelitian menunjukkan rata-rata persentase agregasi trombosit pada kelompok kontrol, penundaan 2 jam, 3 jam, dan 4 jam berturut-turut adalah 56,3%, 59,6%, 71,5%, dan 75,5%. Analisis One-Way Anova menunjukkan nilai signifikansi 0,000 (p<0,05), menandakan adanya perbedaan bermakna antar kelompok. Uji Post Hoc mengungkap bahwa tidak terdapat perbedaan signifikan antara kelompok kontrol dan penundaan 2 jam (p>0,05), sedangkan penundaan 3 dan 4 jam menunjukkan perbedaan yang signifikan (p<0,05). Dengan demikian, pemeriksaan agregasi trombosit masih dapat dilakukan hingga 2 jam setelah pengambilan sampel pada suhu ruang, namun penundaan lebih dari 2 jam tidak direkomendasikan karena dapat memengaruhi akurasi hasil.

Sosialisasi Pencegahan Demam Berdarah Dengue dan Pemeriksaan Hemoglobin Pada Santri Remaja Pondok Pesantren Darul Falah Al Hasyimiyah, Semarang: Community Education on Dengue Hemorrhagic Fever Prevention and Hemoglobin Screening among Students of Darul Falah Al Hasyimiyah Islamic Boarding School, Semarang Putri, Gela Setya Ayu; Rauf, Mohamad Jois; Bano, Siti Maryam; Djafar, Hawin Pratiwi
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. Suppl-1 (2026): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v11iSuppl-1.10936

Dengue Hemorrhagic Fever (DHF) is still a significant public health issue in Indonesia, with a high incidence rate. Overcrowded environments, such as Islamic boarding schools, pose a higher risk for the spread of DHF. In addition to environmental factors, anemia in adolescents also increases susceptibility to infection. Therefore, education on DHF prevention and hemoglobin (Hb) screening is essential as promotive and preventive measures. This activity aimed to raise students' awareness of the importance of maintaining environmental hygiene and to identify hemoglobin status as part of early detection of vulnerability to DHF infection. The program was conducted at Darul Falah Al-Hasyimiyah Islamic Boarding School, Semarang, involving 50 adolescent students. Interventions included interactive counseling on DHF prevention, knowledge evaluation through pre-test and post-test, and Hb measurement using a digital Hb meter. Results showed a significant improvement in students' knowledge, with mean pre-test and post-test scores of 46 and 92, respectively (p<0.05). Hb screening revealed that 64% of participants had Hb levels below normal (mean 10.8±1.2 g/dL), indicating a high prevalence of anemia among students. These findings highlight the need for nutritional interventions, balanced diet education, and regular health monitoring. In conclusion, this activity effectively enhanced students' awareness of DHF prevention while simultaneously identifying anemia status. This model can be duplicated in similar areas as a promotional and preventive strategy against environmentally based infectious diseases. Abbreviations are permissible, except for units of amount, and should be used sparingly.

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