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All Journal Bioma : Berkala Ilmiah Biologi Menara Riau: Jurnal Ilmu Pengetahuan dan Pengembangan Masyarakat Islam MEDIAN Jurnal Biologi Tropis PENDIPA Journal of Science Education BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains JURNAL PENDIDIKAN TAMBUSAI JOURNAL OF SCIENCE AND SOCIAL RESEARCH Menara Ilmu Jurnal Pembelajaran dan Biologi Nukleus JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi Spizaetus: Jurnal Biologi dan Pendidikan Biologi BioEksakta : Jurnal Ilmiah Biologi Unsoed Bioscientist : Jurnal Ilmiah Biologi Jurnal IPTEK Bagi Masyarakat Filogeni: Jurnal Mahasiswa Biologi BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Biologi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Mayasari, Ulfayani
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UTILIZATION OF KECOMBRANG FRUIT PEEL (Etlingera elatior (Jack) RMSm) AS A MOUTHWASH TO PREVENT MOUTH ULCERS AND DENTAL CARIES Rahmadina, Rahmadina; Mayasari, Ulfayani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8591

Abstract

Kecombrang fruit is used by the community as a food ingredient but actually the skin of this kecombrang fruit can be used as a mouthwash for the development of dental caries. This study aims to evaluate the preparation of mouthwash formulations with the addition of kecombrang fruit peel extract. The work procedure starts from extraction, phytochemical testing, antimicrobial testing of extracts, and evaluation of mouthwash preparations. The results contained secondary metabolites in the form of alkaloids, fla-vonoids, tannins, and saponins. Evaluation of mouthwash preparation with pH test is 5.0-5.6, organoleptic test which does not change significantly, viscosity test at 20% and 30% concentration is 1.0193 cP and 1.0061 cP, and antimicrobial test with inhibi-tion diameter on Streptococcus mutans and Candida albicans is 11.3 mm and 11.1 mm. In conclusion, mouthwash with added extracts can inhibit the growth of microbes that cause mouth ulcers and tooth decay.
Optimasi formula salep ekstrak daun sirih hijau (Piper betle L.) sebagai antijamur terhadap Candida albicans dan Malassezia fur fur secara in-vitro Siagian, Irvina; Mayasari, Ulfayani; Nasution, Rizki Amelia
Filogeni: Jurnal Mahasiswa Biologi Vol 5 No 3 (2025): September-Desember
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v5i3.56860

Abstract

Daun sirih hijau adalah salah satu tanaman herbal yang mudah ditemukan dan dibudidayakan di lingkungan masyarakat. Jamur Candida albicans dan Malassezia furfur merupakan penyebab infeksi yang paling umum di Indonesia. Penelitian ini bertujuan untuk mengetahui efektivitas salep yang diformulasikan dari ekstrak daun sirih hijau terhadap pertumbuhan C. albicans dan M. furfur secara in-vitro. Dua teknik eksperimen digunakan dalam penelitian ini: difusi sumur dan difusi Kirby-Bauer. Hasil penelitian menunjukkan bahwa daun sirih hijau mengandung metabolit sekunder flavonoid, alkaloid, terpenoid, steroid, tanin, dan saponin. Ekstrak dari daun sirih hijau mempunyai daya hambat kuat hingga sangat kuat terhadap C. albicans (zona hambat 27,4 ± 3,074 mm) dan M. furfur (25,8 ± 4,010 mm) pada konsentrasi 50%. Sebaliknya, salep ekstrak daun sirih hijau (Piper betle L.) dengan konsentrasi 25% dan 50% hanya menunjukkan daya hambat lemah, yaitu 2,4 ± 0,556 mm dan 7,8 ± 0,378 mm terhadap C. albicans, serta 2,0 ± 1,006 mm dan 4,9 ± 1,761 mm terhadap M. furfur. Penurunan khasiat salep diduga akibat terbatasnya difusi dan stabilitas senyawa aktif selama formulasi. Temuan ini menunjukkan bahwa ekstrak daun sirih hijau berpotensi menjadi antijamur alami yang efisien, namun khasiatnya berkurang bila digunakan sebagai salep.
Effectiveness test of red shoot leaf extract (Syzygium myrtifolium Walp.) as a natural antiperspirant in inhibiting sweat production and the growth of Pseudomonas aeruginosa bacteria that cause body odor Arnita, Lisna; Rasyidah, Rasyidah; Mayasari, Ulfayani
Bioma : Berkala Ilmiah Biologi Volume 28 Issue 1 Year 2026 (Issue in Progress)
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2026.75401

Abstract

Body odor is a problem caused by the activity of microorganisms, one of which is the Pseudomonas aeruginosa bacteria by metabolizing sweat released by the body. The use of antiperspirant products is thought to reduce sweat production and bacterial decay . However, antiperspirants available in the market contain active chemicals that can cause irritation so it is necessary to use plant extracts as an alternative preparation of natural antiperspirant products. This study aims to determine the effectiveness of natural antiperspirant extracts of red shoot leaves (Syzygium myrtifolium Walp.) in inhibiting the growth of Pseudomonas aeruginosa bacteria that cause body odor. The method used is descriptive quantitative with parameters of simplicia and extract yield tests, extract characteristics, phytochemical screening, antibacterial effectiveness of extracts, physical quality tests of antiperspirants, antiperspirant effectiveness and Scanning Electron Microscope (SEM). The results of the study showed that the red shoot leaf extract meets the general standards of medicinal plant extracts and contains secondary metabolite compounds that are able to inhibit the growth of Pseudomonas aeruginosa bacteria with the formulated product producing suitable characteristics as an antiperspirant that is able to inhibit sweat production and the growth of Pseudomonas aeruginosa bacteria which is supported visually by Scanning Electron Microscope (SEM). Based on the results obtained, it is known that the natural antiperspirant red shoot leaf extract (Syzygium myrtifolium Walp.) has antibacterial effectiveness against Pseudomonas aeruginosa . Thus, the use of natural ingredients can be a solution to overcome the causes of body odor and reduce the use of conventional antiperspirant products that contain chemicals that are harmful to health.
Effectiveness of a nasal spray containing essential oil from Citrus microcarpa Bunge on the SEM profile of Aspergillus fumigatus mycelium Ananda, Revian; Mayasari, Ulfayani; Nasution, Rizki amelia
Bioma : Berkala Ilmiah Biologi Volume 28 Issue 1 Year 2026 (Issue in Progress)
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2026.78255

Abstract

Deteriorating air quality during seasonal transitions contributes to the increased growth of pathogenic microorganisms, including Aspergillus fumigatus, which may lead to adverse health impacts. Synthetic antifungal agents may induce resistance and undesirable side effects, thereby promoting interest in safer natural alternatives. This study aimed to evaluate the antifungal effectiveness of a nasal spray formulated with kasturi orange (Citrus microcarpa) peel essential oil against A. fumigatus. The essential oil was extracted through steam distillation and tested at five concentrations to identify the most effective range, after which three concentrations exhibiting the strongest antifungal activity were formulated into a nasal spray. Antifungal effectiveness was assessed using the disc diffusion method, morphological alterations of fungal hyphae were observed using Scanning Electron Microscopy (SEM), and statistical analysis was performed using Analysis of Variance (ANOVA). The results showed that the nasal spray demonstrated significant antifungal activity (p < 0.05), with inhibition zone diameters of 12.8 mm, 16.1 mm, and 20.5 mm at essential oil concentrations of 10%, 20%, and 30%, respectively. Although the highest concentration produced the largest inhibition zone, excessively high essential oil levels do not necessarily improve antifungal performance and may trigger adaptive responses approaching resistance. SEM observations confirmed notable cellular damage, including hyphal lysis, cell wall thinning, and structural shrinkage following exposure to the nasal spray formulation. In conclusion, kasturi orange peel essential oil is effective when formulated as a nasal spray to inhibit A. fumigatus, with 20% identified as the most suitable concentration for further product development.
Antibacterial Activity Test of Patchouli Leaf Essential Oil Facemist (Pogostemon cablin Benth) Against Cutibacterium acnes Bacteria Rahayu, Sri; Mayasari, Ulfayani; Selvia, Irda Nila
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.75402

Abstract

Cutibacterium acnes bacteria are gram-positive anaerobic bacteria that play a role in the pathogenesis of Acne vulgaris (acne). The use of facemist containing natural active ingredients of patchouli leaf essential oil (Pogostemon cablin Benth) can be an alternative in inhibiting the growth of acne-causing bacteria. This study aims to determine the antibacterial activity of patchouli leaf essential oil facemist (Pogostemon cablin Benth) against Cutibacterium acnes bacteria. The method used in making essential oils by steam distillation, chemical compound analysis using Gas Chromatography-Mass Pectrometry (GC-MS) and antibacterial activity testing using the disc diffusion method then formulated into a facemist product and characterized. The results of the study showed that patchouli leaf essential oil had met the standard requirements and contained several of the highest chemical compound components, namely Patchouli alcohol (15.71% and 10.16%), Delta-Guaiene (CAS) (7.14%), Azulene, 1,2,3,4,5,6,7,8-Octahydro-1 (6.48%), 2-Butynyl-5-Hydroxy-3-Oxo-4-Hexanoic Acid Delta-Lactone (5.89%) and 1,1,4,7-Tetramethyldecahydro-1H-Cyclopropa[E] Azulene-4-Ol (5.17%) and the presence of antibacterial activity against Cutibacterium acnes bacteria with concentrations of 10%, 20%, 30%, 40% and 50% with inhibition zone diameters of 11.18 mm, 17.66 mm, 22.36 mm, 23.10 mm and 24.76 mm and the diameter of the antibacterial facemist inhibition zone with concentrations of 20% and 30% obtained results of 20.93 mm and 23.76 mm, then the facemist preparation showed characterization that was in accordance with the product's standard requirements. Based on the test results, it is known that the antibacterial face mist made from patchouli leaf essential oil has the ability to inhibit Cutibacterium acnes bacteria.
Co-Authors Agatha, Cyndy Tri Ananda, Dwika Ananda, Revian ANDINI, DIAH AYU Anggraini, Chorry Dwi Annisa Amalia Ariska, Nila Arnita, Lisna Aulia, Syaikah Nabilah Azhari, Raisa Bahri, Andini Nur Basyaruddin, Nisa Anggraini Damanik, Novita Daulay, Zharifah Zahwa Eliza Rahma, Maudy Eriri, Linda Harahap, Muhammad Rizky Hasriana, Eli Husnarika Febriani, Husnarika Hutagalung, Nuriyah Azami Irda Nila Selvia Kesuma, Luthfiah Ramadhani Khairani, Devi Laylan Syafina Lestari, Yusfa Nanda Lubis, Maulana Hafis Maisyaroh, Dinda Manday, Yola Arfinolita Manik, Siska Pragawati Masyitah, Lidya Angraeni Maulina, Moudy Muhammad Irfan Muhammad Ridwan Nasution, Anggy Yohanna Nasution, Annisa Puji Astuti Nasution, Rahmadani Sandra Nasution, Rizki Amelia Ningsih, Rina Ayu Nurcahyani, Nadya Nurhasanah Siregar . Pratama, Harji Anggia Pratama, Reza Pratiwi, Lini Purba, Gusmita Enda Lorenza Purwaningtyas, Franindya Putri, Reza Malina Putri, Widya Assura R. Tanjung, Linda Fitria Rahmadina, Rahmadina Raissa Amanda Putri Ramadhan, Deviyan Rasyidah Rasyidah Rasyidah Riwanda, Ayu Safitri, Dede Nela Saila, Saila Salsabila, Qothrunnada Shafa Sandi, Najmi Maulidina El Sarah Ramadhani, Sarah Sari, Putri Rasmi Siagian, Irvina Siti Halimah Siti Maimunah Sitorus, Widya Astuti Situmorang, Liza Ardani SRI RAHAYU Syahputri, Sufi Amir Wiwik Sundari Yarashima, Sylvia Zaini, Nurlayla