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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner journal of Basic Medical Veterinary
Estoepangestie, Agnes Theresia Soelih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Total Plate Count of Broiler Meat at Various Market in Bekasi City in March 2021 Firdausyi, Aulia Amatul; Estoepangestie, Agnes Theresia Soelih; Wibawati, Prima Ayu; Hamid, Iwan Sahrial; Solikhah, Tridiganita Intan; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.157-161

Abstract

Food-safety of broiler meat is important because broiler meat is a foodstuff that can be easily contaminated by biological hazard such as bacteria. This study aimed to determine the total plate count of broiler meat at various market in Bekasi City in March 2021. Amount of samples in this study were 40 samples of broiler meat from 10 markets, samples were taken purposively and each sample used 25 grams from Musculus pectoralis of broiler meat. Samples were examined using pour plate method of total plate count. The result showed that 80% (32 of 40 samples) were exceeded the TPC's limit. The highest value of TPC result was 3,7 í— 107 CFU/g from "H” market.
An Evaluation of Nutritional Factors on The Prevalence of Urolithiasis in Cats at The Veterinary Teaching Hospital, Universitas Airlangga During 2017-2019 Mahdi, Daffa Amanul; Sardjana, I Komang Wiarsa; Yudaniayanti, Ira Sari; Estoepangestie, Agnes Theresia Soelih; Triakoso, Nusdianto; Primarizky, Hardany
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.180-184

Abstract

Stones, or uroliths, in the urinary tract cause a disorder called urolithiasis. Patients with urolithiasis may experience blockage and possibly mortality; therefore, prompt treatment is imperative. This study aimed to investigate the prevalence of urolithiasis and its nutritional association in cats treated at the Veterinary Teaching Hospital, Universitas Airlangga during 2017-2019. Data was collected from the secondary resource from the medical report and the factor of feed nutrition involved fiber, protein, fat, and moisture. Data was analyzed using logistic regression to determine the influence of nutrition on the prevalence of urolithiasis in cats in the respective period. The findings indicated that in 2017, urolithiasis prevalence was 0,96%; in 2018, it was 5,55%; and in 2019, it was 0,91%. Meanwhile, it was revealed that the prevalence of urolithiasis was not significantly impacted by feed nutritional variables.
Investigation of Trypanosoma evansi in Sumatran Elephant (Elephas maximus sumatranus) in Indonesia Using Various Methods Purnamasari, Kartika; Nurcahyo, Wisnu; Nursalim, Muhammad Tauhid; Suwanti, Lucia Tri; Estoepangestie, Agnes Theresia Soelih
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.155-162

Abstract

Trypanosoma evansi is a widespread hemoprotozoa that causes trypanosomiasis in both livestock and wildlife. One of the susceptible animals is the Sumatran elephant, which is included in the endangered species category. The infection of this parasite often develops into chronic and sub-clinical forms in elephants, therefore it may become unnoticed and hard to diagnose. This study aimed to analyze and evaluate the infection of T. evansi in semi-captive Sumatran elephants in Way Kambas National Park, Indonesia, using various diagnostic tests. The prevalence of T. evansi in a total of 53 Sumatran elephants was estimated using a card agglutination test for trypanosomiasis (CATT) in 2016. A longitudinal study was later conducted in 2019 using Giemsa stained blood smear (GSBS) and polymerase chain reaction (PCR). The results showed that 26.4% of samples (14/53) were positively detected by both CATT in 2016 and PCR in 2019, while GSBS was unable to detect the parasites in all samples. Furthermore, four individuals were confirmed to have persistent infections. This study concluded that the ability of CATT and PCR were more convincing over GSBS for the diagnosis of sub-clinical trypanosomiasis in Sumatran elephants. However, it is recommended to use a combination of CATT as a screening tool and PCR as a confirmatory test for reliable results.
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Satria, Rafif Galih; Hamid, Iwan Sahrial; Wibawati, Prima Ayu; Estoepangestie, Agnes Theresia Soelih; Saputro, Amung Logam; Praja, Ratih Novita
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan  dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.
Antimicrobial effect of Methanol and Ethanol Extracts of Kembang Bulan (Tithonia diversifolia) Leaves against Staphylococcus aureus Plumeriastuti, Hani; Habibi, Muhammad Afif; Tehupuring, Benjamin Christoffel; Effendi, Mustofa Helmi; Estoepangestie, Agnes Theresia Soelih; Sarudji, Suryanie; Witaningrum, Adiana Mutamsari
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.97-104

Abstract

The purpose of this study was to determine how Staphylococcus aureus responded to Kembang Bulan (Tithonia diversifolia) leaf extract's antibacterial properties. Eight S. aureus bacterial isolates from three Surabaya dairy farms were used in this study. Tithonia diversifolia leaf extract at concentrations of 10%, 5%, 2.5%, 1.25%, and 0% were employed in the study's samples. The antibacterial activity of Tithonia diversifolia leaf extract was assessed in this work using the disk diffusion method and an inhibition zone estimated in millimeters. Based on the findings of this study, it was determined that Tithonia diversifolia leaf extract at 5% and 10% concentrations had 100% antibacterial activity against S. aureus in eight samples. As with S. aureus, Tithonia diversifolia leaf extract at concentrations of 0%, 1.25%, and 2.5% did not exhibit any antibacterial action.
Co-Authors Adiana Mutamsari Witaningrum Amrullah, Amirul Muslim Benjamin Christoffel Tehupuring, Benjamin Christoffel Didik Handijatno Fedik Abdul Rantam Firdausyi, Aulia Amatul Habibi, Muhammad Afif Hani Plumeriastuti Harijani, Nenny Ira Sari Yudaniayanti Iwan Sahrial Hamid Lucia Tri Suwanti, Lucia Tri Mahdi, Daffa Amanul Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nursalim, Muhammad Tauhid Nusdianto Triakoso Praja, Ratih Novita Prastiya, Ragil Angga Primarizky, Hardany Purnamasari, Kartika Raharjo, Dadik Saputro, Amung Logam Sardjana, I Komang Wiarsa Sarudji, Suryanie Satria, Rafif Galih Solikhah, Tridiganita Intan Tacharina, Martia Rani Tyasningsih, Wiwiek Wibawati, Prima Ayu Wisnu Nurcahyo