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All Journal Jurnal Kesehatan Andalas Jurnal Biologi Tropis Majalah Kedokteran Andalas Health and Medical Journal PREPOTIF : Jurnal Kesehatan Masyarakat Journal Orthopaedi and Traumatology Surabaya (JOINTS) Indonesian Journal of Multidisciplinary Science Frontiers on Healthcare Research
Rahmadian, Rizki
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Public Health Social Sciences

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Journal : Frontiers on Healthcare Research

 1 
Unlocking the Potential: A Review of Stem Cell Applications in Regenerative Medicine Musyarifah, Zulda; Rahmadian, Rizki
Frontiers on Healthcare Research Vol. 1 No. 1 (2024)
Publisher : Rumah Sakit Umum Pusat (RSUP) Dr. M. Djamil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63918/fhr.v1.n1.p31-38.2024

Abstract

Background : Regenerative medicine stands at the forefront of transformative healthcare, offering innovative approaches to address tissue damage and degenerative conditions. Central to this paradigm is the profound impact of stem cell therapy, a dynamic and promising avenue within regenerative medicine. As we navigate the complexities of this evolving field, our systematic literature review seeks to unravel the diverse applications, breakthroughs, and challenges inherent in utilizing stem cells for regenerative purposes Methods : We searched in Science direct, Pubmed, NIH and another e-library with keyword stem cell, application and regenerative medicine. Our review aims to contribute to the ongoing narrative of stem cell therapy in regenerative medicine. Through this exploration, we aspire not only to enhance our understanding but also to guide future research endeavors and clinical applications in this dynamic and promising field. Result : We reviewed the article  with the theme article is stem cell and regenerative medicine. Stem cell therapy consistently demonstrated remarkable efficacy in promoting tissue regeneration. Across applications, stem cells showcased a remarkable versatility in enhancing tissue-specific cell behavior and activating regenerative pathways. his versatility positions stem cells as a promising tool with broad applications in regenerative medicine. Conclusion : This review emphasize the versatility and efficacy of stem cells in diverse regenerative scenarios. Evaluating methodological quality and comparing studies reveal nuanced insights, guiding future research directions. This review provides a comprehensive understanding of the current landscape, paving the way for innovative approaches in regenerative medicine.
Growth Factor Identification Based on Speed and Duration of Centrifugation in Platelet Rich Plasma Rahmadian, Rizki; Raymond, Benni; Meksiko, Riki
Frontiers on Healthcare Research Vol. 1 No. 1 (2024)
Publisher : Rumah Sakit Umum Pusat (RSUP) Dr. M. Djamil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63918/fhr.v1.n1.p69-75.2024

Abstract

Background: This study aims to determine the appropriate preparation of simple PRP protocol to yield the maximum growth factor concentration, specifically for PDGF and TGF-β1. Methods: Blood samples were collected from 5 healthy volunteers who signed informed consent for participation in the study. The samples then processed by single centrifugation at 4 four different speed (600, 800, 1000, and 1200 rpm) for 4 different centrifugation times ( 8, 10, 12, 14 minutes). The Platelet Derived Growth Factor (PDGF) and Transforming Growth Factor β1 (TGF- β1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Blood routine test analysis were measured by hematology analyzer. Results: The 8 min with 600 rpm centrifugation protocol resulted in a slightly greater release of TGF-β1 (7,127 ng/mL) and PDGF (473,909 pg/mL). While the other samples resulted from 50,296 pg/mL (14 min with 1200 rpm) until 406.883 pg/mL (8 min with 800 rpm) of PDGF. The other results of TGF-β1 vary from 0,558 ng/mL (14 min with 1200 rpm) to 6,322 ng/mL (10 min with 600 rpm). Conclusion: The highest concentration of PDGF and TGF-β1 were obtained from centrifugation process at 600 rpm for 8 minutes. Meanwhile, the lowest concentration of PDGF and TGF-β1 were obtained from centrifugation process at 14 min with 1200 rpm.
Histological Comparison of Lyophilized Amniotic Membrane, Tulle Gras Dressing and Topical Gentamicin on Acute Partial Thickness Wound : In Vivo Study Raymond, Benni; Fadila, Srigunda Arisya; Rahmadian, Rizki; Tofrizal, Tofrizal
Frontiers on Healthcare Research Vol. 1 No. 2 (2024)
Publisher : Rumah Sakit Umum Pusat (RSUP) Dr. M. Djamil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63918/fhr.v1.n2.p1-7.2024

Abstract

Background: Partial thickness wounds refer to the damage that extends from the epidermis to the upper layers of dermis. This study aims to compare the histological regrowth of acute partial thickness wounds following application of lyophilized amniotic membrane, tulle grass dressing and topical gentamicin. This study aims to establish a standard approach for treating partial thickness wound. Methods: Three groups of Mus Musculus mice were used in our experimental study. Similar to harvesting split thickness skin grafts, partial thickness wounds are performed. The wound was then treated with lyophilized amniotic membrane, tulle grass dressing and topical gentamicin. Using the Scoring System for Histological Assessment of Wound Healing, the wound is histopathologically examined at day 14. In this study, One way Anova was used for statistical analysis. Results: Application of lyophilized amniotic membrane showed different histological characteristic of healing from other method, with p=0.001 it showed significance epidermal growth compared to topical gentamicin. Whereas dermal thickness were also better, accompanied by lower dermal collagen density. Amniotic membrane has a large source of stem cells and contains several growth factors which is important in the physiological process of wound healing and tissue regeneration. Conclusions: Characteristic of skin healing using lyophilized amniotic membrane showed rapid epidermal growth and gave impression of less fibrosis tissue, so that would be potential for better wound treatment especially for prevention of scar.
Co-Authors Akbar, Inasa Faiza Ananda, Gita Putri Andani Eka Putra Arni Amir Dessy Arisanty Dina Arfiani Rusjdi Edison, Ebill Fuji Eka Fithra Elfi, Eka Fithra Eka Sahputra, Roni Eti Yerizel Fadila, Srigunda Arisya Firdawati, Firdawati Fortuna, Fory Hanum, Fathiya Juwita Hirowati Ali, Hirowati Ida Rahmah Burhan Intan, Shinta Ayu Irrahmah, Miftah Kaharudin Kaharudin Lili Irawati Marlina Meksiko, Riki Mustafa Noer Musyarifah, Zulda Rahmatini . Rahmawati, Meilisa Ramda Ilhami, Yose Rasya, Salsabila Faiha Wiendra Raymond, Benni Rikarni Rikarni Rinaldiansyah Sahputra, Roni Eka Selfi Renita Rusjdi Selfi Renita Rusjdi, Selfi Shafira, Zahra Silvia, Nelmi Siregar, Muhammad Abi Ghoffari Siti Nurhajjah, Siti Tofrizal Yanti, Farah Sevnell