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Gusti A. Y. Lestari
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Veterinary

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Pengaruh Substitusi Jagung Giling dan Dedak Padi dengan Tepung Sabut Kelapa Muda Hasil Fermentasi Khamir (Saccromyces Cerevisiae) terhadap Fermentasi Rumen In Vitro: The Effect of Substituion of Milled Corn and Rice Bran with Young Coconut Flour Fermented by Yeast (Saccromyces cerevisiae) on Rumen Fermentation in Vitro. Yosefa Lestari Mansay; Marthen Yunus; Gusti Ayu Yudiwati Lestari
Jurnal Peternakan Lahan Kering Vol. 3 No. 1 (2021): Maret
Publisher : Jurnal Peternakan Lahan Kering

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh substitusi jagung giling dan dedak padi dengan tepung sabut kelapa muda hasil fermentasi khamir Saccharomyce cerevisiae dalam pakan konsentrat terhadap konsentrasi pH rumen, VFAdanNH3 secara in vitro.Metode yang digunakan dalam penelitian ini adalah metode percobaan menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan 3 ulangan.Adapun perlakuan dalam penelitian ini adalah P0; pakan konsentrat tanpa tepung sabut kelapa muda fermentasi (kontrol), P1=substitusi jagung giling dan dedak padi dengan 10% tepung sabut kelapa muda fermentasi Saccharomyces cerevisiae dalam pakan konsentrat; P2=substitusi jagung giling dan dedak padi dengan 20% tepung sabut kelapa muda fermentasi Saccharomyces cerevisiae dalam pakan konsentrat; P3=substitusi jagung giling dan dedak padi dengan 30% tepung sabut kelapa muda fermentasi Saccharomyces cerevisiae dalam pakan konsentrat. Data yang diperoleh dianalisis menggunakan Analisis Of Variance(ANOVA). Dari  penelitian yang dilakukan diperoleh hasil rataan pH  P0= 6,73±0,03 P1= 6,67±0,06 P2= 6,53±0,03 P3= 6,80,±0,06 rataan konsentrasi VFA (mM) P0= 95,01±7,13 P1= 95,50±2,00 P2= 97,30±6,87 P3= 97,88±6,99 rataan konsentrasi NH3(mM) P0= 8,52±0,03 P1=8,11±0,06 P2= 8,55±0,03 P3= 8,50±0,06 Hasil analisis statistik menunjukkan bahwa perlakuan berpengaruh tidaknyata (P>0.05) terhadap konsentrasi pH rumen VFA,dan NH3 secara in vitro. Dari penelitian ini dapat disimpulkan bahwa  penggunaan tepung sabut kelapa muda terfermentasi dalam pakan konsentrat tidak memberikan pengaruh nyata terhadap konsentrsipH  rumen VFA, dan NH3 Kata kunci : Fermentasi, Saccharomyces cerevisiae, Sabut Kelapa Muda, VFA, NH3 pH   The purpose of this study was to determine the effect of substitution of milled corn and rice bran with coconut husk flour fermented yeast Saccharomyce cerevisiae in concentrate feed on the rumen pH, concentrations of VFA and NH3in vitro. The method used in this study was an experimental method using a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments in this study were P0; concentrate feed without fermented young coconut husk flour (control), P1 = substitution of milled corn and rice bran with 10% coconut husk fermented Saccharomyces cerevisiae flour in concentrate feed; P2 = substitution of milled corn and rice bran with 20% coconut husk flour fermented Saccharomyces cerevisiae in concentrate feed; P3 = substitution of milled corn and rice bran with 30% coconut husk flour fermented Saccharomyces cerevisiae in concentrate feed. The data obtained were analyzed using Analysis Of Variance (ANOVA). From the research conducted, it was obtained that the average pH value was P0 = 6.73 ± 0.03 P1 = 6.67 ± 0.06 P2 = 6.53 ± 0.03 P3 = 6.80, ± 0.06 mean VFA concentration (mM). ) P0 = 95.01 ± 7.13 P1 = 95.50 ± 2.00 P2 = 97.30 ± 6.87 P3 = 97.88 ± 6.99 mean NH3 concentration (mM) P0 = 8.52 ± 0, 03 P1 = 8.11 ± 0.06 P2 = 8.55 ± 0.03 P3 = 8.50 ± 0.06 The results of statistical analysis showed that the treatment had no significant effect (P> 0.05) on the rumen pH concentration of VFA, and NH3in vitro. From this research it can be concluded that the use of fermented young coconut coir flour in concentrate feed did not have a significant effect on the rumen pH concentrations of VFA and NH3. Keywords: Fermentation, Saccharomyces cerevisiae, Young Coconut Coir, VFA NH3, pH
Article Pengaruh level Saccharomieces cerevisiae sebagai inokulum dalam fermentasi tepung sabut kelapa muda terhadap kandungan energi, selulosa, hemiselulosa dan Total Digestible Nutrien (TDN) Meki Fiktor Otu; Mariana Nenobais; Gusti Ayu Yudiwati Lestari
Jurnal Peternakan Lahan Kering Vol. 2 No. 4 (2020): Desember
Publisher : Jurnal Peternakan Lahan Kering

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Abstract

Penelitian ini dilaksanakan di Laboratorium Kimia Pakan Fakultas Peternakan Universitas Nusa Cendana Kupang, selama 3 minggu. Tujuan penelitian untuk mengetahui pengaruh fermentasi tepung sabut kelapa muda dengan level Saccharomyeces cerevisiae yang berbeda terhadap perubahan kandungan energi, selulosa,  hemiselulosa dan TDN (Total Digestible Nutrient) . Metode eksperimen yang dirancang adalah Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan tiga ulangan sehingga terdapat 12 unit percobaan. Adapun perlakuan dalam penelitian ini yaitu R0: Tepung sabut kelapa muda tanpa fermentasi (kontrol), R1: Tepung sabut kelapa muda fermentasi kamir Saccharoyces cerevisiae 5 % (berat/berat), R2: Tepung sabut kelapa muda fermentasi kamir Saccharomyces cerevisiae 10 % (berat/berat). R3: Tepung sabut kelapa muda fermentasi Saccharomyces cerevisiae 15 % (berat/berat). Hasil analisis sidik ragam menunjukkan perlakuan berpengaruh nyata (P<0,05) terhadap kandungan selulosa dan TDN tetapi perlakuan berpengaruh tidak nyata (P>0,05) terhadap kandungan  Energi dan  Hemiselulosa. Hasil uji lanjut Duncan menunjukan bahwa antar  pengaruh perlakuan R0:R3, R1:R2, R1:R3 dan R2:R3 berbeda nyata (P<0,05) terhadap kandungan selulosa dan R0:R1, R0:R2 dan R0:R3,R1:R2,R2:R3 berbeda nyata (P<0,05) terhadap kandungan TDN. Disimpulkkan bahwa pemberin saccharomieces cerevisiae sebagai inokulum dalam fermentasi tepung sabut kelapa muda dapat meningkatkan kandungan energi dan TDN, menurunkan kandungan selulosa dan hemiselulosa dan perlakuan terbaik adalah pada level 10% Saccharomieces Cerevisiae. Kata Kunci: sabut kelapa, Saccharomieces, Energi, Selulosa, Hemiselulosa, TDN   The study was conducted at the Chemistry Feed Laboratory of the Faculty of Animal Science, Nusa Cendana University, Kupang for 3 weeks. The purpose of this study was to evaluate the effect of the duration of fermentation of young coconut husk with different Saccharomyeces cerevisiae levels on changes in energy content, cellulose, hemicellulose and TDN. The material used were ground young coconut husk. Completely randomized design (CRD) 4 treatments with 3 replicates resulting 12 experimental units. The treatments were R0: young coconut husk flour without fermentation (control), R1: young coconut husk flour fermented withr Saccharoyces cerevisiae 5% (w/w), R2: young coconut flour flour fermented with Saccharomyces cerevisiae 10% (w/w), and R3: Young coconut coir fermentation Saccharomyces cerevisiae 15% (wt/w). The variables observed in this study were energy, cellulose, hemicellulose, and TDN content. Statistical analysis shows that the effect of treatment is significant effect (P <0.05) on Energy, Cellulose and TDN content but not significant (P> 0.05) on Hemicellulose content. Duncan's multiple range test shows that differences between treatments R0: R1, R0: R3 and R1: R3 are significant effect (P <0.05) in the energy content, R0: R2, R0: R3, R1: R2, R1: R3 and R2 : R3 are significant effect (P <0.05) in the content of Cellulose, and R0: R1, R0: R2 and R0: R3 are significant effect (P <0.05) in TDN content. The conclusion is that the effect of different levels of saccharomieces cerevisiae as an inoculum in the fermentation of young coconut fiber flour 10% treatment can increase the energy content and TDN but can reduce the cellulose and hemicellulose content in the fermentation of young coconut husk flour. Keywords: coconut husk, Energy, Cellulose, Hemicellulose, TDN
Fraksi Serat Silase Campuran Sorgum (Sorghum bicolor (L) Moench) dan Kelor (Moringa oleifera Lam) yang Ditanam pada Jarak Tanam Berbeda: Fiber Fraction of Mixed Silage Sorgum (Sorghum bicolor (L) Moench) and Moringa (Moringa Olifera Lam) Planted At Different Planting Distances Maria Suryanti Nenu; Markus Miten Kleden; Gusti Ayu Yudiwati Lestari; Mariana Nenobais
Jurnal Peternakan Lahan Kering Vol. 5 No. 4 (2023): Desember
Publisher : Universitas Nusa Cendana

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Abstract

This research was conducted with the aim of looking at the silage fiber fraction in a mixture of sorghum and moringa grown at different distances from moringa. This study used an experimental method with a completely randomized design (CRD) design with four trials and four replications, namely: P0: Sorghum silage without moringa, P40: Sorghum + Moringa silage with a spacing of Moringa 40 x 40 cm, P60: Sorghum + Moringa silage with Moringa silage 40 x 40 cm, P60: Sorghum + Moringa silage with Moringa plant spacing 60 x 60 cm, P80 : Sorghum + Moringa silage with Moringa plant spacing 80 x 80 cm. The variables measured were neutral detergent fiber (NDF), acid detergent fiber (ADF), and lignin. The research showed that threre was variation NDF, ADF and lignin content of sorgum-moringa silage of each treatment. Satistical analysis showed that the treatment had no effect on NDF and ADF content while it had contrary on lignin content. It can be concluded that more wide distance planted of moringa more growth rate and production of moringa forage and it had an effect on fiber fraction of silage while distance plated of moringa 80x80 cm was the best distance planted spacing with shorgum at distance planted spacing of shorgum 40x40 cm Riset ini dilakukan dengan tujuan untuk melihat fraksi serat silase pada campuran sorgum dan kelor yang ditanam pada jarak yang berbeda dari kelor. Penilitian ini menggunakan metode eksprimen desain Rancangan acak lengkap (RAL) dengan empat percobaan dan empat ulangan, yaitu: P0: Silase sorgum tanpa kelor, P40: silase sorgum + kelor dengan jarak tanam kelor 40 x 40 cm, P60: Silase sorgum + kelor dengan jarak tanam kelor 60 x 60 cm, P80 : Silase sorgum + kelor dengan jarak tanam kelor 80 x 80 cm. Variabel yang diukur adalah neutral detergent fiber (NDF), acid detergent fiber (ADF), dan lignin. Hasil penelitian menunjukkan bahwa adanya variasi kandungan NDF, ADF dan lignin silase sorgum-kelor pada masing-massing perlakuan. Hasil analisis statistik menunjukkan bahwa perlakuan berpengaruh tidak nyata terhadap NDF dan ADF namun berpengaruh nyata terhadap kandungan lignin. Dapat disimpulkan bahwa semakin lebar jarak tanam kelor, kemampun kelor untuk hidup dan berproduki lebih tinggi dan memberikan kontribusi terhadap fraksi serat silase yang ada dengan jarak tanam kelor 80x80 cm merupakan jarak tanam terbaik yang ditanam bersama sorgum dengan jarak tanam sorgum 40x40 cm.
Pengaruh Lama Waktu Biofermentasi Chromolaena odorata dengan Sumber Karbon Tepung Putak terhadap Kualitas Fisik Tamelab, Nikanor; Oematan, Gustaf; Lestari, Gusti Ayu Yudiwati
Animal Agricultura Vol 2 No 2 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i2.93

Abstract

This study aims to determine the effect of the length of biofermentation time of Chromolena odorata with a carbon source of putak flour on physical quality (color, odor / aroma, texture, mold, pH and temperature). The method used is an experimental method with a complete randomized design (RAL) with 4 treatments and 4 repeats so that there are 16 experimental units. The treatment used consisted of Lb21: Biofermentation duration 21 days (as control), Lb14: Biofermentation duration 14 days, Lb7: Biofermentation duration 7 days, and Lb0: Biofermentation duration 0 days. The results of statistical analysis showed that the length of biofermentation time of Chromolaena odorata with the carbon source of putak flour had a very real effect (P<0.01) on color, aroma and temperature but a real effect (P>0.05) on texture, fungal density and pH. From the results of the study, it was concluded that the length of biofermentation time of Chromolaena odorata silage 14 days provides excellent color value and the length of biofermentation time of Chromolaena odorata 7 days which gives excellent odor / aroma and temperature value.
Pengaruh Lama Waktu Biofermentasi Chromolaena Odorata dengan Sumber Karbon Tepung Putak terhadap Kandungan Serta Kecernaan Protein Kasar dan Lemak Kasar In vitro Nomleni, Meri; Dami Dato, Twen O.; Lestari, Gusti A. Y.; Mullik, Marthen L.
Animal Agricultura Vol 2 No 3 (2025): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i3.108

Abstract

The purpose of this study was to determine the effect of the length of biofermentation time of chromolena odorata with a carbon source of putak flour on the content and digestibility of crude protein and crude fat in vitro. The research method used is an experimental method (experimental) with a Complete Random Design pattern consisting of 4 treatments and 4 repeats so that there are 16 experimental units. The treatment used is LB21: biofermentation duration 21 days (As control), LB14: biofermentation duration 14 days, LB7: biofermentation duration 7 days, LB0: biofermentation duration 0 days. The data were obtained and analyzed by variety analysis (ANOVA), and continued with the Duncan multiple distance test to determine the effect between treatments. The results of statistical analysis showed that the length of biofermentation of Chromolaena odorata with the carbon source of putak  flour had a real effect (P<0,01) On crude protein content, crude fat content and crude protein digestibility in vitro, while on crude fat digestibility in vitro the effect is not real (P<0,05). It can be concluded that the length of biofermnetation of Chromolaena odorata with putak flour source affects crude protein content, crude fat and gauze protein digestibility in vitro but does not affect crude fat digestibility in vitro.  
Pelatihan Pengembangan Hijauan Pakan di Kelompok Peternak Mekar Desa Merbaun Kecamatan Amarasi Barat Kabupaten Kupang Lestari, Gusti Ayu Yudiwati; Hartati, Erna; Malelak, Gemini
GOTAVA Pengabdian Kepada Masyarakat Vol. 1 No. 2 (2023): GOTAVA Jurnal Pengadian Kepada Masyarakat
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/jpmgotava.v1i2.7

Abstract

This training activity aims to introduce the participants to various types of forage that can be used as feed for ruminant livestock, especially cattle. Another objective is to motivate the participants to innovate in feed provision, ultimately encouraging them to start agricultural enterprises, particularly in forage production, to support the development of cattle farming in the Amarasi Barat sub-district of Kupang Regency. The training begins with providing information about different types of forage, planting methods, harvesting age, and post-harvest handling. The methods used to achieve these objectives include counseling, demonstrations, direct practice, mentoring, and evaluation. The training participants are members of the Mekar livestock group in Dusun VI (Puru), Merbaun Village – Kecamatan Amarasi Barat. The results from the mentoring and evaluation activities will be used as a reference for creating future programs related to post-harvest forage technology. After community members have acquired the knowledge and abilities of cattle raising, the community's revenues will provide better outcomes, increase income, and improve welfare in the long run.
Nutritional Profile and In Vitro Rumen Fermentation of Mixed Silage of Kume Grass and Moringa Leaves Kleden, Markus Miten; Nenobais, Mariana; Lestari, Gusti Ayu Yudiwati; Ratu, Maria Rosdiana Deno
Jurnal Sain Peternakan Indonesia Vol 20 No 3 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.3.165-172

Abstract

A study was conducted to determine the nutritional profile and in vitro fermentation characteristics of mixed silage of Kume grass and Moringa leaves in the feed chemistry laboratory, Department of Animal Science, Faculty of Animal Husbandry, Fisheries and Marine, Universitas Nusa Cendana. The materials used were Kume grass and Moringa leaves, with the addition of rice bran as a fermentation stimulant. The research used an experimental method with a completely randomised design consisting of four treatments and five replications. The treatments were the proportions of Kume grass and Moringa leaves, respectively: KK0 = 100% Kume grass + 0% Moringa leaves; KK25 = 75% Kume grass +  25% Moringa leaves;  KK50 = 50% Kume grass + 50% Moringa leaves and KK75 = 25% Kume grass + 75% Moringa leaves. The parameters measured included organoleptic quality, silage nutrient content, in vitro digestibility, and rumen fermentation. The results showed that increasing the level of Moringa leaves resulted in good ensilage, indicated by the absence of mould and a sour aroma. Rising levels of moringa leaves had a statistically significant effect (P<0.01) on the nutrient content of silage, rumen fermentation, and in vitro digestibility of the silage. It can be concluded that ensiling technology using Moringa leaves offers a promising solution to address feed shortages.
Pengaruh Lama Waktu Biofermentasi Chromolaena Odorata dengan Sumber Karbon Tepung Putak Terhadap Produksi Gas Methan, pH, NH3 dan VFA In-vitro Saefatu, Markus E.; Oematan, Gustaf; Lestari, Gusti A. Y.; Dami Dato, Twen O.
Animal Agricultura Vol 3 No 2 (2025): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v3i2.132

Abstract

The aims of this study was to determine the effect of the length of time of Chromolaena odorata biofermentation with putak flour carbon source on methane production, pH, NH3 and Total VFA in-vitro. The main materials used were Chromolaena odorata, putak flour, and rumen fluid.  The method used was an experimental method with a completely randomised design (CRD) pattern consisting of 4 treatments and 4 replications. The treatments were LBf21 = 21 days biofermentation duration (as control); LBf14 = 14 days biofermentation duration; LBf7 = 7 days biofermentation duration; LBf0 = 0 days biofermentation duration. The variables studied were Methane Production, pH, NH3 and VFA in-vitro.  The results of statistical analysis on the variables of methane production, NH3 and total VFA showed that the treatment had no significant effect (P>0.05), while the pH value statistically showed that the treatment had a significant effect (P<0.05) on the length of time of biofermentation of Chromolaena odorata silage. It is concluded that the length of time of the biofermentation process has not been able to increase the production of total VFA, NH3, and reduce methane production but is able to increase the pH value of Chromolaena odorata silage silage from the biofermentation process.
Pengaruh Pemberian Silase Pakan Komplit Berbasis Sorgum-Clitoria Ternatea Dengan Penambahan Konsentrat Mengandung ZnSO4 Dan ZnCu Isoleusinat Terhadap Kadar Metabolit Darah Kambing Kacang Nubatonis, Imron Alberto; Hartati, Erna; Lestari, Gusti A. Y.; Nenobais, Mariana
Animal Agricultura Vol 1 No 3 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v1i3.21

Abstract

The aim of this research was to determine the effect of providing complete feed silage based on sorghum-Clitoria ternatea with the addition of concentrate containing ZnSO4 and Zn-Cu isoleucinate on the blood metabolites of Kacang goats.  This research used 12 Kacang goats aged 12 months with an average body weight of 13.21 kg (KV=14.01%).  This research used an experimental method with a randomized block design (RAK) with 4 treatments and 3 replications.  The four treatments are T0: sorghum-Clitoria ternatea silage without concentrate, T1: complete feed silage containing 10% concentrate, T2: complete feed silage containing 20% concentrate, and T3: complete feed silage containing 30% concentrate, each treatment contains  150 ZnSO4 kg concentrate and Zn-Cu kg Isoleucinate ration.  The results of this study showed that giving complete feed silage with the addition of concentrate containing ZnSO4 and Zn-Cu isoleucinate did not have a significant effect (P>0.05) on cholesterol levels, glucose levels, total plasma protein levels and blood urea levels in Kacang goats.  In conclusion, giving complete feed silage based on sorghum-Clitoria ternatea with the addition of concentrate containing ZnSO4 and Zn-Cu isoleucinate does not affect cholesterol levels, glucose levels, total plasma protein levels and blood urea levels in Kacang goats.
Konsentrasi Metabolit Darah Sapi Persilangan Ongole X Brahman yang Diberi Pakan Silase Rumput Kume dan Alysicarpus vaginalis Pada Imbangan yang Berbeda Bees, Daud Ambraham; Lestari, Gusti A. Y.; Maranatha, Grace
Animal Agricultura Vol 1 No 3 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v1i3.25

Abstract

The aim of this study was to determine the effect of blood metabolite concentrations on Ongole x Brahman cross cattle fed kume grass silage and Alycicarpus vaginalis at different balances. The livestock used were 4 Ongole x Brahman cattle with an average body weight of 167.25 kg (KV = 15.6%) with an age range of 8-12 months and a body weight of 171,5-200 kg. This research was carried out using the experimental method with the Latin square design (RBSL) with 4 treatments and 4 replications. The treatments in this study were AV0 = Silage 70% Kume Grass + 30% concentrate, AV20 = Silage 56% Kume Grass + 14% Alysicarpus vaginalis + 30% Concentrate, AV40 = Silage 42% Kume Grass + 28% Alysicarpus vaginalis + 30% Concentrate, AV60 = Silage 28% Kume Grass + 42% Alysicarpus vaginalis + 30% Concentrate. Data were analyzed using analysis of variance (ANOVA). The results showed blood glucose concentrations AV0=69.94±2.83 mg/dl, AV20=76.23±2.23 mg/dl, AV40= 74.57±6.41 mg/dl, AV60=71.96±2.51 mg/dl. Blood Urea concentration AV0= 42.08±1.74mg/dl, AV20= 42.08±180mg/dl, AV40= 40.93±1.24mg/dl, AV60= 43.46±0.75mg/dl. Total Plasma Protein AV0= 6.55±0.30mg/dl, AV20= 6.35±0.34mg/dl, AV40= 6.35±0.34mg/dl, AV60= 6.80±0.00mg/dl. Based on the analysis of variance, it showed that the treatment had no significant effect (P>0.05) on blood glucose, blood urea and total plasma protein. It was concluded that the administration of kume grass silage and Alysicarpus vaginalis with different balances showed relatively the same results on blood glucose concentration, blood urea concentration and total plasma protein in Ongole x Brahman cross cattle so that they could be used as alternative feed.
Co-Authors Adrianus Safarius Radja Angga Dwi Firmanto Arnol E. Manu Bees, Daud Ambraham Cesilia Wilfrida Seran Dami Dato, Twenfosel O. Diana Meliani Sabat Elisabeth metodia botha Enawati, Luh Sri Engelbertus D. P. Fernandez Erna Hartati Erna Hartati Erna Hartati Geertuida M. Sipahelut Gemini Ermiani Mercurina Malelak I Gusti Ng. Jelantik Imanuel Benu Jalaludin Jelantik, I Gusti Ngurah Kii, Petrus Renoldy Kleden, Markus Miten Leady H.S. Ratu luju, imeldiana linda M.R. Deno Ratu Maranatha, Grace Maria Suryanti Nenu Mariana Nenobais Mariana Nenobais Marthen Yunus Martina Raga Lay Meki Fiktor Otu Mullik, M. L. Nenobais, Mariana Ni Nengah Suryani Nikolaus, Tara T. Nomleni, Meri Nubatonis, Imron Alberto Oematan, Gustaf Osan, Elfiana Frida Saefatu, Markus E. Saskia Radja Say, Yohanes K. Sefrans Banamtuan Selvister B. Womakal Tamelab, Nikanor Tandang, Mariano D. Tsael, Yulvi Lidia Twen O. Dami Dato Yosefa Lestari Mansay