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All Journal Jurnal Sain Peternakan Indonesia AGRIVITA, Journal of Agricultural Science JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Bakti Masyarakat Indonesia Journal of Tropical Animal Science and Technology Jurnal Nukleus Peternakan Jurnal Peternakan Lahan Kering Animal Agricultura

Kleden, Markus Miten

Universitas Nusa Cendana

Author-ID : 528676

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Electrical & Electronics Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Veterinary Other

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Evaluation of Digestability and Rumen Parameters Through In-vitro Concentrate Containing Binahong Flour as Secondary Compound A. A. Widu; F. U. Datta; M. M. Kleden
Jurnal Sain Peternakan Indonesia Vol 16, No 4 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.4.362-367

This study was conducted to determine the effect of different levels of binahong flour used in concentrate feed on dry matter digestibility (DMD), organic matter digestibility (OMD), VFA and NH3 concentrations in vitro. The design used was a completely randomized design with 4 treatments and 4 replications. The treatments studied were: R0; concentrate feed without binahong flour, R1; concentrate feed contains 10% binahong flour, R2; concentrate feed containing 20% binahong flour, R3; concentrate feed contains 30% binahong flour. Based on the results of the study, the average DMD (%) R0 73.56±0.75, R1 74.50 ±0.61, R2 74.98±0.70, R3 76.65±0.90, OMD (%) R0 69.50±1.10, R1 69.82±1.00, R2 70.07±0.88, R3 72.61±0.99, VFA concentration (mM) R0 108.53±8.52, R1 110.75±6.51, R2 111.40±2.35, R3 112.77 ±14.76, NH3 concentration (mM) R0 9.74±0.74, R1 10.61±0.44, R2 11.55±0.41, R3 11.55±0.33. The results of statistical analysis confirmed that the treatment had a very significant effect on the increase in DMD, OMD, NH3 (P<0,01), but not significant on the total VFA concentration. The conclusion of this study is that the use of binahong flour up to a level of 30% in concentrate feed has an effect on increasing in DMD, OMD, VFA, and NH3 concentrations in vitro.

Genetic Diversity Evaluation of Moringa oleifera, Lam from East Flores Regency Using Marker Random Amplified Polymorphic DNA (RAPD) and Its Relationship to Chemical Composition and In Vitro Gas Production Markus Miten Kleden; Hendrawan Soetanto; Kusmartono Kusmartono; Kuswanto Kuswanto
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.1027

The research objective was to evaluate the genetic diversity of Moringa oleifera, Lam (MO) and its relationship to chemical composition and in vitro gas production (IVGP). Fresh MO leaves were kept frozen in ice gels pack until laboratory analysis. Four methods applied: RAPD marker for measuring DNA concentration and purification; Kjeldhal and HPLC for analysing proximate and amino acid (AA) composition; and IVGP. MO’s four distinct morphology found: green, red, reddish green and aromatic green. RAPD result analysis was 68.8-74.7 %, it means those MO had a close genetic similarity. The morphological differences are also related to leaves chemical composition variation. The highest protein and AAs content were found in aromatic green MO. Total IVGP at 96 hours reached 95.9, 99.3, 111, 115 mL per 500 mg DM in aromatic green, green, reddish green, red MO, respectively and statistically among those was highly significant difference (P<0.01). However, DM and OM digestibility did not differ significantly and estimated ME contents were similar suggesting MO leaves had sufficient fermentable nitrogen amount required to ensure rumen microbes normal activities. Conclusively, those MO has a close genetic relationship but the aromatic green MO more beneficial due its higher content of crude protein and AAs.

PRODUKSI, KOMPOSISI BOTANI DAN KAPASITAS TAMPUNG HIJAUAN PADA PADANG PENGGEMBALAAN ALAM AWAL MUSIM KEMARAU (Production, botanical composition and carrying capacity of forage in native grassland at early dry season) Ventryan Haryanto Hae; Markus Miten Kleden; Stefanus Tany Temu
JURNAL NUKLEUS PETERNAKAN Vol 7 No 1 (2020): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i1.2299

Grassland area must be managed and record existing condition doe to as a source of cheapest feed and easy to find out. The aim of this study was to measure production, botanical composition and carrying capacity of forage in native grassland area at early dry season. This study was carried out in Maubokul village, Pandawai sub district, district of East Sumba. Forage production measured by actual weight estimate method with 1m x 1m of frame as tool utilization. Data collected than analyzed to find out production, botanical composition and carrying capacity of forage in native grassland. Statistical analysis showed that the value of Summed Dominance Ratio (SDR) was 84.4% for grass, 15.6% for legume and 0% for weeds. Dry matter production was 1.866,8 kg/ha with carrying capacity value was 1.05AU/Ha/year. As a conclusion that grassland area at Maubokul village was dominated of grass and carrying capacity was high.

KECERNAAN NUTRIEN DAN PARAMETER RUMEN PAKAN KONSENTRAT YANG MENGANDUNG TEPUNG DAUN KERSEN SEBAGAI PENGGANTI JAGUNG SECARA IN VITRO (In vitro nutrient digestibility and rumen parameter of concentrate feed base chery leaf meal as corn substitution) Kristina Jhena; Markus M. Kleden; Imanuel Benu
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.3018

The present study aimed to determine the effect of concentrate base cherry leaves (Muntingia calabura L) substituting corn meal on in vitro nutrient digestibility and rumen parameter. This study was conducted in the laboratory of feed chemistry Faculty of animals Husbandry University of Nusa Cendana. The experiment was subjected to a completely randomized design (CRD) with 4 treatments and 3 replications. The tested treatments were: MC0 = concentrate containing 100% corn meal without cherry leaf meal; MC20 = concentrate containing 80% corn meal + 20% cherry leaf meal; MC40 = concentrate containing 60% corn meal + 40% cherry leaf meal; MC60 = concentrate containing 40% corn meal + 60% cherry leaf meal. The result showed that corn replacement by muntingia calabura leaves meal in concentrate feed decrease nutrient digestibility. In contrary the rumen parameter had increase value. Statistical analysis showed that the the parameter measured was very high depend on the Muntingia leaves meal utilization. As a conclusion that Muntingia calabura leaves can be used as corn substitution up to 20%.

PENGARUH SUPLEMENTASI SILASE CAMPURAN SORGHUM- Clitoria ternatea PADA BERBAGAI LEVEL KONSENTRAT MENGANDUNG ZnSO4 DAN Zn-Cu ISOLEUSINAT TERHADAP FERMENTASI RUMEN KAMBING KACANG (Effects of supplemented mixed silage of sorghum-clitoria ternatea with.....) Adrianus Safarius Radja; Markus M. Kleden; Gusti A. Y. Lestari
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.3019

The purpose of this study was to determine the effect ofsupplemented mixed silage of sorghum-Clitoriaternatea with different levels of concentrate containing ZnSO4 and Zn-Cu Isoleusinate on rumen fermentation of kacang goats. Twelve buck kacang goats aged 12-14 months with initial body weight ranged 11-19 kg andthe average was 14,40 kg while 14,93% coefficient of variation was used in this reseach. Methode used was experiment methode with block randomized design as research design consist of four treatments and three replication. The treatmenten were T1 = mixed silage of sorghum-Clitoriaternatea providing, T1, T2 and T3 was T1 supplemen by concentrate with level of 10, 20 and 30 % containing of 150 mg ZnSO4 and 2% Zn-Cu Isoleusinat respectively. Parameter measured was rumen pH value, NH3 and total of VFA concentration. The data obtained was analyzed by analysis of variance (ANOVA) and followed by Least Significant Different test. The results of statistical analysis showed that supplementation with various levels of concentrates containing 150 mg ZnSO4 and 2% Zn-Cu Isoleucine had no significant effect (P> 0.05) on rumen pH and NH3 concentration, while significantly efect (P <0.05) on VFA totalt. It can be concluded that supplementation with various concentrates containing ZnSO4 and Zn-Cu Isoleucicate produced relatively the same pH and NH3 and VFA tended to increase, with the best concentration of concentrate supplementation at 30%.

PENGARUH PENGGUNAAN BERBAGAI LEVEL KONSENTRAT MENGANDUNG ZnSO4 dan Zn-Cu ISOLEUSINAT TERHADAP PENGGUNAAN ENERGI KAMBING KACANG YANG MENGKONSUMSI SILASE SORGHUM–Clitoria ternatea (The effect of different level of concentrate additioncontaining ZnSo4.... Norbertus Bria; Erna Hartati; Markus M. Kleden
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4220

The aim of this research was to measure the effect of different level of concentrate addition containing znso4 and zn-cu isoleusinat on energy utilization of kacang goats fed mixed silage of sorghum-clitoria ternatea. Twelve male kacang goats with average body weight 14.4 kg and 14.93 % coefficient of variation was used in this research. Experiment methode was used with research design was block randomised design consist of 4 treatments and 3 replications. The treatments were: T1: mixed silage of sorghum-Clitoriaternatea without concentgrate; T2; T3 and T4 was mixed silage of sorghum-Clitoriaternatea added by 10, 20 and 30 % of concentrate containg of 150 mg ZnSO4 and 2 % of Zn-Cu isoleusinat respectively. Parameter measured was consumnption and digestibility of anergy and blood glucose concentration. The result showed that addition of different level of concentrate containing ZnSO4 and Zn-Cu isoleusinatfollowed by increase of consumption and digestibility of energy and blood glucose concentration. It can be concluded the amount of concentrate providing containing of ZnSO4 and Zn-Cu isoleusinathad effect on energy consumption while no effect on digestibility of energy and blood glucose concentration. Further more 20 % of concentrate containg of ZnSO4 and Zn-Cu isoleusinat was the greatest level.

PENGARUH PEMBERIAN KONSENTRAT YANG MENGANDUNG TEPUNG DAUN KELOR DENGAN LEVEL YANG BERBEDA TERHADAP PENGGUNAAN NITROGEN KAMBING LOKAL L. S. Enawati; Markus M. Kleden; Marfandi M. Robo
Journal of Tropical Animal Science and Technology Vol 1 No 1 (2019): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

The study aimed to determine the effect of the use of feed concentrates containing Moringa leaf flour on protein consumption, protein digestibility and rumen ammonia concentration. Data were analyzed using a randomized block design (RBD) with 4 treatments and 3 replications, and continued with the Duncan test. The treatments in this study include: K1: Concentrate without the use of Moringa leaf flour; Concentrate with the addition of 10% Moringa leaf flour; K3: Concentrate with the use of Moringa leaf flour 20%; and K4: Concentrate with the use of 30% Moringa leaf flour. The variables determined were Rough Protein Consumption and Digestion and rumen NH3 Concentration. The results showed that protein (g / tail / day) consumption and crude protein digestibility (%) were highest in the treatment of K4: 36.37 and 72.01, then followed by K3: 35.81 da. 71.93; K2: 35.06 and 69.85 and K1: 31.99 and 59.91. The highest rumen NH3 (mM) concentration is also occupied by K4: 19.77 followed; K3: 18.24; K2: 16.48 and K1: 11.16. The results of statistical analysis showed that the treatment had no significant effect (p> 0.05) on consumption and had a significant effect (p<0.05) on the digestibility coefficient of crude protein and rumen NH3. It was concluded that the use of Moringa leaf flour as a constituent component to the level of 30% can be used for animal feed to increase consumption and nutrient digestibility of livestock as well as the provision of rumen NH3.

Upaya Pendayagunaan Limbah Pertanian sebagai Pakan Unggulan Musim Kemarau di Lahan Kering Markus Miten Kleden; Mariana Nenobais
Jurnal Pemberdayaan Masyarakat Vol 3 No 1 (2018): Mei
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

Limitation of feed and low nutrient consumption in dry season cause a longer period of cattle fattening. The problems can be overcome by applying a cheaper and easier technology namely feed conservation technology. Method applied were extension, training, and mentoring. The result showed that the farmers’ participation were very active refers to their present and questiones raised deal with the materials presented. The highly participation also found during the training and mentoring since the feed conservation technology was an innovative activity and high benefit. The activities result were two units of representative model cattle cage due to clean and hygienic aspects, one unit of feed storage, 400 kg corn straw silage, 300 kg rice straw ammoniation, and 100 kg concentrate.

PENERAPAN IPAT-BO GUNA MENINGKATKAN PRODUKTIVITAS PADI PADA LAHAN KERING DI ERA REVOLUSI INDUSTRI 4.0 Erna Hartati; Twen O. Dami Dato; G.A.Y. Lestari; Markus M. Kleden
Jurnal Bakti Masyarakat Indonesia Vol 3, No 1 (2020): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Rice is a major issue in food security and independence because more than 90% of the population in Indonesia is highly dependent on rice. The need for rice is increasing as the population exponentially grows, but the area of paddy fields is decreasing. In the era of the industrial revolution 4.0, one of the goals was to increase the knowledge and skills of the farming community. In this connection, the attention of the government and the community is focused on increasing rice production through various innovations and the use of rice intensification technology based on the use of organic fertilizer. Innovation use of ABG (Amazing Bio Growth) biostimulant fertilizer with a target of achieving 8-15 tons/ha of rice compared to conventional 3-4 tons/ha. Efforts to increase rice production with the technology "Organic-Based Aerobic Controlled Rice Intensification (IPAT-BO)" is the answer to support the industrial revolution 4.0 in agriculture. The success of IPAT-BO technology in irrigated rice fields and rainfed rice fields apparently can also be applied in rice fields that use live water or water from bore wells on dry land in semi-arid tropical ecosystems in NTT. IPAT-BO technology applied to farmers in Bipolo Village, Sulamu and Babau Districts, East Kupang District, Kupang Regency using limited water along with drainage arrangements on dry land, grain production can reach 6-8 tons/ha. The successful application of this technology is highly dependent on the development of the root system, biodiversity, and balance in the supply of nutrientsABSTRAK:Beras merupakan isu utama dalam ketahanan dan kemandirian pangan karena lebih dari 90% penduduk di Indonesia sangat tergantung pada beras. Kebutuhan beras semakin meningkat seiring pertumbuhan eksponensial penduduk, namun luas lahan sawah semakin berkurang. Di era revolusi industri 4.0 salah satu sasarannya adalah peningkatan pengetahuan dan keterampilan masyarakat tani. Dalam kaitannya dengan hal tersebut, perhatian pemerintah dan masyarakat difokuskan untuk meningkatkan produksi padi melalui berbagai inovasi dan penggunaan teknologi intensifikasi padi berbasis pada penggunaan pupuk organik. Terobosan penggunaan biostimulan pupuk ABG (Amazing Bio Growth) dengan target pencapaian produksi padi 8-15 ton/ha dibanding dengan produksi 3-4 ton/ha secara konvensional. Upaya peningkatan produksi padi dengan teknologi “Intensifikasi Padi Aerob Terkendali Berbasis Organik (IPAT-BO)” merupakan jawaban untuk mendukung revolusi industri 4.0 di bidang pertanian. Keberhasilan teknologi IPAT-BO di lahan sawah irigasi dan sawah tadah hujan ternyata juga dapat diterapkan di lahan sawah yang menggunakan air hidup atau air dari sumur bor pada lahan kering di ekosistem tropis semi kering di NTT. Teknologi IPAT-BO yang diterapkan pada petani di Desa Bipolo, Kecamatan Sulamu dan Babau, Kecamatan Kupang Timur, Kabupaten Kupang dengan menggunakan air secara terbatas disertai pengaturan drainase di lahan kering, produksi gabahnya dapat mencapai 6-8 ton/ha. Keberhasilan penerapan teknologi tersebut sangat tergantung pada perkembangan sistem perakaran, keanekaragaman hayati dan keseimbangan pasokan nutrisi.

The PENGARUH PENGGANTIAN TEPUNG IKAN DENGAN TEPUNG DAUN KELOR DALAM KONSENTRAT TERHADAP METABOLIT DARAH TERNAK KAMBING YANG DIBERI PAKAN SILASE RUMPUT KUME DAN DAUN GAMAL (Effect of replacing fish meal by moringa leaf meal in the concentrate on blood..... Maria F. Dj. Putri; Markus M. Kleden; Daud Amalo
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.4795

This research aimed to evaluate the effect of replacing fish meal by moringa leaf meal in the concentrate on blood metabolite of goats fed silage of kume grass-gliriciadia leaf mixture. Four male kacang goats aged 1-1.5 year with average initial body weight of 21.14 ± 1.06 kg and coeficient variation 4.13 % were used in this experiment. The experimental design was a latin square consisting of 4 treatments and 4 periode as replication. The treatments were K0 = silage mixture of kume grass-gliricidia leaf + concentrate with containing 100% of fish meal; K25 = mixture silage of kume grass-gliricidia leaf + concentrate with containing 75% of fish meal and 25% of moringa leaf meal; K50 = mixture silage of kume grass-gliricidia leaf + concentrate with containing 50% of fish meal and 50% of moringa leaf meal; K75 = mixture silage of kume grass-gliricidia leaf + concentrate with containing 75% of fish meal and 25% of moringa leaf meal. Results showed that treatment had no effect (P>0,05) on blood glucose, ureum, cholesterol and total protein plasm. It can be concluded that moringa leaf meal can be used as fish meal replacement up to 75%.

Co-Authors A. A. Widu Adrianus Safarius Radja Banu, Selmi Rosanti Daud Amalo Enawati, L. S. Erna Hartati Erna Hartati G.A.Y. Lestari Gusti A. Y. Lestari Hendrawan Soetanto Imanuel Benu Jelantik, I Gusti Ngurah Jemiman, Maria Asri Anita Kristina Jhena Kusmartono Kusmartono Kuswanto Kuswanto Lestari, Gusti A.Y Lestari, Gusti Ayu Y Lestari, Gusti Ayu Y. Lola, Mario Johanes M D.S Randu, M D.S M. R.T Ratu, M. R.T M.R. Deno Ratu Marfandi M. Robo Maria F. Dj. Putri Maria Suryanti Nenu Mariana Nenobais Mariana Nenobais Mullik, Yelly M Nenobais, Mariana Norbertus Bria Oematan, Gustaf Stefanus Tany Temu Tanggela, Friscalia I. Theofilus Odolfus Milo Twen O. Dami Dato Ventryan Haryanto Hae

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