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VARIASI GENETIK IKAN KERAPU (Epinephelus sp) Di TPI GABION MEDAN BELAWAN BERDASARKAN DNA MITOKONDRIA Hasra, Barratun Nisa; Idami, Zahratul; Manalu, Kartika
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page203-210

Abstract

Background: Genetic variation can share data on genetic diversity in fish populations. mtDNA is used to determine the kinship between grouper fish originating from TPI Gabion Medan Belawan. This study aims to obtain the latest genetic variations found in grouper populations (Epinephelus sp.), analyze for the kinship relationship between grouper populations (Epinephelus sp.), determine for the kinship relationship between grouper fish from TPI Gabion Medan Belawan and grouper fish that already exist in NCBI.Methods: This study used experimental methods.Results: The results of research on species of the genus Epinephelus namely Epinephelus fuscoguttatus, Epinephelus sexfasticus, and Epinephelus areolatus obtained by processing the sequencing data by analyzing nucleotide characters using the Mega 11 application. All three samples have low GC Content, it is said that all three samples have primitive properties. All three samples have the same genetic distance because they have a genetic distance value (Pairwise distance) below 3%.Conclusion: Reconstruction of phylogenetic trees shows that grouper kinship (phylogenetics) originating from TPI Gabion Medan Belawan with grouper fish that already exist in NCBI have close kinship and common ancestor
DNA BARCODE KERANG KEPAH (Polymesoda Sp.) DI BAGAN PERCUT DAN DANAU SIOMBAK SUMATERA UTARA MENGGUNAKAN GEN CYTOCHROME OXIDASE SUB UNIT 1 (CO1) Utami, Mutiara Alya; Idami, Zahratul; Manalu, Kartika
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page194-202

Abstract

Background: DNA Barcode is a molecular technique using short sequences of nucleotide bases that aims to identify and classify living things. This study aims to obtain nucleotide characters from DNA barcode results from kepah clams using CO1 genes in Sumatran waters, to determine the results of molecular identification of species of kepah clams (Polymesoda sp.) found in North Sumatra waters, and to determine the kinship relationships of kepah clam species (Polymesoda sp.) in North Sumatra waters. Samples were obtained in Bagan Percut and Lake Siombak, carried out from July to August 2023 at the Biomolecular Laboratory, Medan State University. Methods: The method used is to use DNA barcodes with CO1 genes. Results: The results of research of species of the genus Polymesoda sp. namely Polymesoda erosa and Polymesoda expansa obtained from processing sequencing data by analyzing nucleotide characters using the Mega 11 application, namely variations in the percentage of AT content (62.69%), GC content (37.56%) for Polymesoda erosa species and AT content (62.42%), GC content (38.64%) for Polymesoda expansa species, which means both species have primitive properties. Molecular identification of the genus Polymesoda sp. obtained from the results of DNA sequence samples along 369 bp so that it is suitable to be used as DNA barcodes. The genetic distance between the species studied is Polymesoda erosa (0.000) and Polymesoda expansa (0.920) which means they have a close genetic distance. Conclusion: Phylogenetic tree reconstruction carried out using the Neighbour Joining method shows that between the two species Polymesoda erosa (Bagan Percut) and Polymesoda expansa (Lake Siombak) have a close kinship and come from a common ancestor.
Isolasi dan Identifikasi Mikroba Penyebab Kontaminasi dari Udara di Laboratorium Kultur Jaringan Tumbuhan UIN-SU Medan Sucahyo, Ahmad Imam; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Vol. 1 No. 1 (2023): November
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/biology.v1i1.1931

Abstract

Plant tissue culture laboratory is a technique for growing cells, tissues or slices of plant organs which are often called explants in the laboratory in an artificial media containing aseptic (sterile) nutrients to become a whole plant. This research aims to know the isolation and identification of contaminating microbes in the plant tissue culture laboratory and to know the types of contaminating microbes in the plant tissue culture laboratory. This research was conducted from december 2022 to january 2023 at the Wahdatul Ulum plant tissue culture laboratory, uinsu medan with 5 main sampling points.the sampling point consists of an incubation room, planting room and practicum space which is divided into 3 collection points. Staining tests and biochemical tests are conducted at the USU faculty of mathematics and natural sciences microbiology laboratory. The results of the study found 23 bacterial isolates that contamined the wahdatul ulum plant tissue culture laboratory at UINSU Medan consisting of the genera bacillus, lactobacillus, streptobasil, aeromonas, pseudomonas, serratia, bacteroides and acinetobacter while the contaminating fungi were Mucor sp. Contamination occurs due to mobility and activity in the laboratory room.
Development of ‘Truth or Dare’ Team Games Tournament-Based Biology Media for Teaching Digestive System in Senior High School Kinanti, Aulia Andin; Manalu, Kartika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7635

Abstract

Background: Low student engagement and learning outcomes in biology, particularly in abstract topics such as the human digestive system, are often caused by the use of monotonous learning media that do not actively involve students. This research aims to create Truth or Dare learning media integrated with the Team Games Tournament (TGT) model to enhance the learning outcomes of eleventh-grade high school students on the topic of the digestive system. Methodology: This study uses the Research and Development (R&D) method with the 4D model (Define, Design, Develop, Disseminate). Validation was conducted by subject matter experts and media experts, while practicality and effectiveness were tested through student and teacher response questionnaires, as well as pre- and post-tests. Findings: Validation results indicated that the media was highly valid, with a score of 98% for the subject matter and 82,1% for the media. Teacher and student responses indicated that the media was highly practical, with scores of 95,5% and 90,4%, respectively. The N-Gain analysis showed a high level of effectiveness, with a score of 0,76. This media has proven capable of improving student learning outcomes while fostering active, collaborative, and enjoyable engagement in learning. Thus, the Truth or Dare-based TGT learning media is suitable as an innovative alternative in Biology education to enhance student learning outcomes, particularly for abstract topics such as the human digestive system. Contribution: These results demonstrate the potential contribution of game-based learning in biology education as an alternative to foster student-centered and meaningful learning
PENGEMBANGAN LEMBAR KERJA PESERTA DIDIK (LKPD) BERBASIS GUIDED INQUIRY PADA MATERI SISTEM SARAF Arbaiyah, Seri; Anas, Nirwana; Manalu, Kartika
Jurnal Review Pendidikan dan Pengajaran Vol. 8 No. 2 (2025): Volume 8 No. 2 Tahun 2025
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v8i2.45908

Abstract

Penelitian ini bertujuan untuk mengembangan lembar kerja peserta didik (LKPD) berbasis guided inquiry pada materi sistem saraf. Penelitian dilakukan pada bulan Juli 2024 di SMA Negeri 1 Batang Kuis. Penelitian ini menggunakan metode penelitian dan pengembangan (R&D) model Thiagarajan (4D). Instrumen penelitian yaitu lembar angket, untuk validator ahli materi, validato ahli media, pendidik, peserta didik, kepraktisan dan uji kelayakan. Subjek uji coba pada penelitian yaitu 29 peserta didik dari kelas XI IPA 4 SMA Negeri 1 Batang Kuis untuk mengetahui penilaian LKPD melalui angket respon peserta didik dan uji kepraktisan untuk mengetahui kepraktisan LKPD kepada 10 peserta didik melalui angket uji kepraktisan. Hasil validasi oleh ahli materi diperoleh presentase 91,1%. Hasil validasi oleh ahli media diperoleh presentase 95,5% atas kriteria sangat layak. Hasil respon pendidik diperoleh presentase 92,1% atas kriteria sangat layak. Hasil respon peserta didik terhadap LKPD diperoleh presentase 88,5%. atas kriteria sangat layak. Hasil uji kepraktisan diperoleh presentase 93,7% atas kriteria sangat praktis. Hasil dari keefektifan diperoleh dari ketuntasan belajar mencapai nilai KKM yaitu 80 diperoleh presentase 93,1% berada pada kriteria sangat efektif. Dengan demikian, lembar kerja peserta didik (LKPD) berbasis guided inquiry pada materi sistem saraf sudah layak dipergunakan pada saat proses pembelajaran.
Development of Socio-Scientific Issues Based Reproduction E-Module to Enhance Critical Thinking Skills Amalia, Fitri; Manalu, Kartika
Jurnal Mangifera Edu Vol 10 No 1 (2025): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v10i1.243

Abstract

This research focuses on the development of an e-module integrating the Socio-Scientific Issues (SSI) approach in teaching human reproductive system material to enhance critical thinking skills among 11th-grade students at SMAS Cerdas Murni. The study was motivated by the low level of students' critical thinking skills, the predominance of conventional teacher-centered methods, and the lack of interactive and contextual learning materials that can connect concepts to real-life issues. The research employed a Research and Development (R&D) design with the 4D model (Define, Design, Develop, and Disseminate). The scope was limited to the development stage due to time and budget constraints. The developed SSI-based e-module was validated by subject matter experts, yielding a feasibility score of 96.43%, and by media experts, yielding a score of 95.31%, both categorized as “highly feasible.” Practicality testing by biology teachers and students resulted in percentages of 96.67% and 95.33%, respectively, indicating the e-module is “very practical” for classroom use. Effectiveness was measured through an N-Gain analysis, producing a score of 0.80, which falls into the “high” category and demonstrates a significant improvement in students’ critical thinking skills. These findings indicate that the SSI-based e-module is feasible, practical, and effective in supporting the development of critical thinking skills, providing an innovative and engaging alternative to conventional biology teaching on the human reproductive system. The module has potential for broader implementation in senior high school biology learning.
Pengembangan E-LKPD Berbasis Problem Based Learning (PBL) dalam Meningkatkan Keterampilan Berpikir Kritis Pada Materi Sistem Peredaran Darah Kelas XI IPA di SMA Negeri 1 Lau Baleng Maha, Destri Br; Manalu, Kartika
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17575

Abstract

This study aims to develop an E-LKPD based on Problem Based Learning (PBL) by utilizing the Liveworksheet platform to improve the critical thinking skills of grade XI IPA students at SMA Negeri 1 Lau Baleng. The study used the Research and Development (R&D) method with the 4D model (Define, Design, Develop, Disseminate). The research subjects involved 31 grade XI IPA F2 students and one biology teacher. The product was validated by material and media experts with results of 90.62% and 88.75%, respectively (very valid category). The practicality test showed a teacher response of 100% and a student response of 95.72% (very practical category). The effectiveness of E-LKPD was demonstrated through the N-Gain analysis = 0.74 (high category). The research findings indicate that (1) the results of expert validation on content, media, and assessment items fall into the Very Valid category; (2) teacher responses reached 100% and student responses 95.72%, both categorized as Very Practical; (3) the results of the pretest and posttest showed an improvement in critical thinking skills with an N-Gain score of 0.74 (high category). Therefore, the PBL-based E-LKPD using Liveworksheet is feasible, practical, and effective for use in biology learning on the circulatory system material.
Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria Annisa, Rafika; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6334

Abstract

Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.
Ultilazation of Bakteria Pseudomonas aeruginosa and Bacillus Subtilos as Bioremediation of Metal Pb in Textile Waste Hasibuan, Ira Aqrabi Br; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6820

Abstract

The Indonesian textile industry is developing to meet people's needs for clothing. 3916 industries in Indonesia exist in 2021, but the number of these industries is not balanced with liquid waste processing. One alternative way to reduce heavy metal levels in waste is bioremediation. The aim of this research is to determine how incubation time and the number of Pseudomonas aeruginosa and Bacillus subtlis bacteria influence the reduction of Pb metal levels in textile waste. Several steps include the bioremediation process, colony counting, measuring Pb metal levels, measuring temperature levels, measuring pH, and data analysis. Bioremediation results: Pseudomonas aeruginosa and Bacillus subtilis bacteria succeeded in reducing Pb metal levels in textile waste with an incubation time of 14 days. The results show that this bacterium is able to reduce Pb metal levels in textile waste with the addition of a 1:1 concentration of 56%, a 1:2 concentration of 86%, and a 2:1 concentration of 92. These results show that this bacterium is successful as a bioremediation agent in reducing Pb metal levels in waste.
Population and Characterization of Rhyzospheric Bacteria of Pineapple Plant (Ananas comosus L. Merr) on The Highland Land of Lumban Sihite Village, Regency Dairi Ginting, Monika; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6822

Abstract

The rhizosphere is the part of the soil around the roots of plants which is known to have a greater number of rhizosphere bacteria compared to the soil far from the roots. This research aims to determine the population and characteristics of rhizosphere bacteria from pineapple plants (Ananas comosus L. Merr) on the highland land of Lumban Sihite village, Dairi district. The stages used in this research were taking samples at three different points, then carrying out multilevel dilutions, inoculating them into petri dishes filled with PCA (Plate Count Agar) media, then counting the growing colonies using the TPC (total plate count) method, purification, morphological characterization, gram staining and biochemical tests. The research results obtained at point 3 had the largest population of 463.96 x 10⁴ CFU/g, while point 1 had the lowest population, namely 20.07 x 10⁴ CFU/g. Based on the results of morphological, gram staining and biochemical characterization of bacterial isolates, the isolates were identified as belonging to the genus Azospirillum, Bacillus, Pseudomonas and Azotobacter.