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All Journal JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Jurnal Mangifera Edu Jurnal Review Pendidikan dan Pengajaran (JRPP) Biopendix: Jurnal Biologi, Pendidikan dan Terapan Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi Spizaetus: Jurnal Biologi dan Pendidikan Biologi JURNAL BIOSHELL Bioscientist : Jurnal Ilmiah Biologi Jurnal Simki Pedagogia TEACHING : Jurnal Inovasi Keguruan dan Ilmu Pendidikan Bioed : Jurnal Pendidikan Biologi Jurnal Pijar MIPA Pedagogika: Jurnal Ilmu-Ilmu Kependidikan Jurnal Biologi
Manalu, Kartika
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Physics Public Health Social Sciences Other

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PENGEMBANGAN LEMBAR KERJA PESERTA DIDIK (LKPD) BERBASIS GUIDED INQUIRY PADA MATERI SISTEM SARAF Arbaiyah, Seri; Anas, Nirwana; Manalu, Kartika
Jurnal Review Pendidikan dan Pengajaran Vol. 8 No. 2 (2025): Volume 8 No. 2 Tahun 2025
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v8i2.45908

Abstract

Penelitian ini bertujuan untuk mengembangan lembar kerja peserta didik (LKPD) berbasis guided inquiry pada materi sistem saraf. Penelitian dilakukan pada bulan Juli 2024 di SMA Negeri 1 Batang Kuis. Penelitian ini menggunakan metode penelitian dan pengembangan (R&D) model Thiagarajan (4D). Instrumen penelitian yaitu lembar angket, untuk validator ahli materi, validato ahli media, pendidik, peserta didik, kepraktisan dan uji kelayakan. Subjek uji coba pada penelitian yaitu 29 peserta didik dari kelas XI IPA 4 SMA Negeri 1 Batang Kuis untuk mengetahui penilaian LKPD melalui angket respon peserta didik dan uji kepraktisan untuk mengetahui kepraktisan LKPD kepada 10 peserta didik melalui angket uji kepraktisan. Hasil validasi oleh ahli materi diperoleh presentase 91,1%. Hasil validasi oleh ahli media diperoleh presentase 95,5% atas kriteria sangat layak. Hasil respon pendidik diperoleh presentase 92,1% atas kriteria sangat layak. Hasil respon peserta didik terhadap LKPD diperoleh presentase 88,5%. atas kriteria sangat layak. Hasil uji kepraktisan diperoleh presentase 93,7% atas kriteria sangat praktis. Hasil dari keefektifan diperoleh dari ketuntasan belajar mencapai nilai KKM yaitu 80 diperoleh presentase 93,1% berada pada kriteria sangat efektif. Dengan demikian, lembar kerja peserta didik (LKPD) berbasis guided inquiry pada materi sistem saraf sudah layak dipergunakan pada saat proses pembelajaran.
Development of Socio-Scientific Issues Based Reproduction E-Module to Enhance Critical Thinking Skills Amalia, Fitri; Manalu, Kartika
Jurnal Mangifera Edu Vol 10 No 1 (2025): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v10i1.243

Abstract

This research focuses on the development of an e-module integrating the Socio-Scientific Issues (SSI) approach in teaching human reproductive system material to enhance critical thinking skills among 11th-grade students at SMAS Cerdas Murni. The study was motivated by the low level of students' critical thinking skills, the predominance of conventional teacher-centered methods, and the lack of interactive and contextual learning materials that can connect concepts to real-life issues. The research employed a Research and Development (R&D) design with the 4D model (Define, Design, Develop, and Disseminate). The scope was limited to the development stage due to time and budget constraints. The developed SSI-based e-module was validated by subject matter experts, yielding a feasibility score of 96.43%, and by media experts, yielding a score of 95.31%, both categorized as “highly feasible.” Practicality testing by biology teachers and students resulted in percentages of 96.67% and 95.33%, respectively, indicating the e-module is “very practical” for classroom use. Effectiveness was measured through an N-Gain analysis, producing a score of 0.80, which falls into the “high” category and demonstrates a significant improvement in students’ critical thinking skills. These findings indicate that the SSI-based e-module is feasible, practical, and effective in supporting the development of critical thinking skills, providing an innovative and engaging alternative to conventional biology teaching on the human reproductive system. The module has potential for broader implementation in senior high school biology learning.
Pengembangan E-LKPD Berbasis Problem Based Learning (PBL) dalam Meningkatkan Keterampilan Berpikir Kritis Pada Materi Sistem Peredaran Darah Kelas XI IPA di SMA Negeri 1 Lau Baleng Maha, Destri Br; Manalu, Kartika
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17575

Abstract

This study aims to develop an E-LKPD based on Problem Based Learning (PBL) by utilizing the Liveworksheet platform to improve the critical thinking skills of grade XI IPA students at SMA Negeri 1 Lau Baleng. The study used the Research and Development (R&D) method with the 4D model (Define, Design, Develop, Disseminate). The research subjects involved 31 grade XI IPA F2 students and one biology teacher. The product was validated by material and media experts with results of 90.62% and 88.75%, respectively (very valid category). The practicality test showed a teacher response of 100% and a student response of 95.72% (very practical category). The effectiveness of E-LKPD was demonstrated through the N-Gain analysis = 0.74 (high category). The research findings indicate that (1) the results of expert validation on content, media, and assessment items fall into the Very Valid category; (2) teacher responses reached 100% and student responses 95.72%, both categorized as Very Practical; (3) the results of the pretest and posttest showed an improvement in critical thinking skills with an N-Gain score of 0.74 (high category). Therefore, the PBL-based E-LKPD using Liveworksheet is feasible, practical, and effective for use in biology learning on the circulatory system material.
Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria Annisa, Rafika; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6334

Abstract

Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.
Ultilazation of Bakteria Pseudomonas aeruginosa and Bacillus Subtilos as Bioremediation of Metal Pb in Textile Waste Hasibuan, Ira Aqrabi Br; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6820

Abstract

The Indonesian textile industry is developing to meet people's needs for clothing. 3916 industries in Indonesia exist in 2021, but the number of these industries is not balanced with liquid waste processing. One alternative way to reduce heavy metal levels in waste is bioremediation. The aim of this research is to determine how incubation time and the number of Pseudomonas aeruginosa and Bacillus subtlis bacteria influence the reduction of Pb metal levels in textile waste. Several steps include the bioremediation process, colony counting, measuring Pb metal levels, measuring temperature levels, measuring pH, and data analysis. Bioremediation results: Pseudomonas aeruginosa and Bacillus subtilis bacteria succeeded in reducing Pb metal levels in textile waste with an incubation time of 14 days. The results show that this bacterium is able to reduce Pb metal levels in textile waste with the addition of a 1:1 concentration of 56%, a 1:2 concentration of 86%, and a 2:1 concentration of 92. These results show that this bacterium is successful as a bioremediation agent in reducing Pb metal levels in waste.
Population and Characterization of Rhyzospheric Bacteria of Pineapple Plant (Ananas comosus L. Merr) on The Highland Land of Lumban Sihite Village, Regency Dairi Ginting, Monika; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6822

Abstract

The rhizosphere is the part of the soil around the roots of plants which is known to have a greater number of rhizosphere bacteria compared to the soil far from the roots. This research aims to determine the population and characteristics of rhizosphere bacteria from pineapple plants (Ananas comosus L. Merr) on the highland land of Lumban Sihite village, Dairi district. The stages used in this research were taking samples at three different points, then carrying out multilevel dilutions, inoculating them into petri dishes filled with PCA (Plate Count Agar) media, then counting the growing colonies using the TPC (total plate count) method, purification, morphological characterization, gram staining and biochemical tests. The research results obtained at point 3 had the largest population of 463.96 x 10⁴ CFU/g, while point 1 had the lowest population, namely 20.07 x 10⁴ CFU/g. Based on the results of morphological, gram staining and biochemical characterization of bacterial isolates, the isolates were identified as belonging to the genus Azospirillum, Bacillus, Pseudomonas and Azotobacter.
Esting of Fungal Antagonists from The Rhyzosphere of Mother-in-Law's Tongue (Sansevieria trifasciata Prain) as a Fungal Control Agent Fusarium sp. Julianty, Dwita; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7522

Abstract

Fusarium sp. is a serious problem for various plant commodities because of its ability to act as a pathogen and cause damage to plant anatomy and morphology. The rhizosphere fungus of the mother-in-law's tongue plant has more abundant inhibitory mechanisms and is able to directly inhibit hyphae growth and suppress the growth of pathogenic fungi, so it was chosen as a biocontrol agent in this research. The aim of this research was to determine the antagonistic properties and ability of the rhizosphere fungus from the mother-in-law's tongue plant (Sansevieria trifasciata Prain) against the fungus Fusarium sp. This research was carried out with data analysis starting with sterilization and making media, then taking soil samples from the rhizosphere of the mother-in-law's tongue using the purposive random sampling to continue with the isolation and purification stage of the rhizosphere fungus of the mother-in-law's tongue plant. Antagonist test of fungi from the rhizosphere of snake plant against the fungus Fusarium sp. carried out using a double culture method (dual culture). The data obtained were then analyzed using Microsoft Excel 2013 to obtain an average inhibition which shows the potential of fungi from the rhizosphere of the plants as controlling agents for the fungus Fusarium sp. The results of isolation and antagonistic tests, the five isolates of snake plant rhizosphere fungus (Sansevieria trifasciata Prain) have varying inhibitions ranging from very weak to moderate. The highest inhibition against Fusarium sp. was shown by isolate JDT12, while the lowest inhibition was shown by isolate JDT11. The antagonism mechanism found by isolate JDT12 is the antibiosis mechanism.
PENGEMBANGAN LKPD BERBASIS CONTEXTUAL TEACHING AND LEARNING BERMUATAN POTENSI LOKAL TANAMAN BUAH PADANGSIDEMPUAN PADA MATERI KEANEKARAGAMAN HAYATI KELAS X MAN 1 PADANGSIDEMPUAN Siregar, Abdul Hasym Mazhurin; Manalu, Kartika; Herni, Zulfiana
TEACHING : Jurnal Inovasi Keguruan dan Ilmu Pendidikan Vol. 5 No. 3 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/teaching.v5i3.7327

Abstract

This research aims to find out the feasibility, practicality and effectiveness of CTL- based LKPD containing the local potential of Padangsidimpuan fruit plants in biodiversity material for class 4D (Define, Design, Develop, and Disseminate). The instruments used in the research were teacher and student needs analysis sheets, material expert and media expert validation sheets, teacher and student response questionnaire sheets, and test questions in the form of pretest and posttest to measure students' understanding of biodiversity material. The validity of the LKPD obtained from material experts was 89% and media experts were 100% in the very valid category. The practicality of the LKPD obtained from the biology teacher response questionnaire was 85% in the very practical category and 84.33% of the students' responses were in the very practical category. The effectiveness of the LKPD was obtained through test questions in the form of pretest and posttest with an N-Gain presentation of 0.73% in the quite effective category with a percentage of 73% in the high category. The three aspects of validity, practicality and effectiveness with a large percentage show that Contextual Teaching and Learning-based LKPD contains local potential for biodiversity material that is suitable for use in learning activities. ABSTRAKPenelitian ini bertujuan untuk mengetahui bagaimana kelayakan, kepraktisan dan keefektifan dari LKPD berbasis CTL bermuatan potensi lokal tanaman buah Padangsidimpuan pada materi keanekaragaman hayati kelas X MAN 1 Padangsidimpuan sebagai bahan ajar yang dapat membantu guru dalam melaksanakan pembelajaran Biologi serta membantu Peserta didik untuk memahami materi keanekragaman hayati dengan cara yang mudah dan meningkatkan pengetahuan peserta didik mengenai keberadaan tanaman buah yang ada di Padangsidempuan serta menjaga kelestariannya.. Metode penelitian ini menggunakan Research and Development (RnD) dengan model 4D (Define, Design, Develop, and Disseminate). Instrumen yang digunakan dalam penelitian berupa lembar analisis kebutuhan guru dan peserta didik, lembar validasi ahli materi dan ahli media, lembar angket respon guru dan peserta didik, dan soal test berupa pretest dan posttest untuk mengukur pemahaman siswa mengenai materi keanekaragaman hayati. Validitas LKPD yang didapatkan dari ahli materi adalah sebesar 89% dan ahli media sebesar 100% dengan kategori sangat valid. Kepraktisan LKPD diperoleh dari angket respon guru biologi 85% dengan kategori sangat praktis dan peserta didik 84,33% dengan kategori sangat praktis. Keefektifan LKPD diperoleh melalui soal test berupa pretest dan posttest dengan presentasi N-Gain 0,73% kategori cukup efektif dengan persentare 73% dengan kategori tinggi. Ketiga aspek validitas, kepraktisan dan keefektifan dengan presentase yang besar menunjukkan bahwasannya LKPD berbasis Contekstual Teaching and Learning bermuatan potensi lokal materi Keanekaragaman hayati layak untuk dipergunakan dalam kegiatan pembelajaran.
Isolasi dan Identifikasi Escherichia coli pada Sumber Air Minum di Desa Banjar Toba Kecamatan Berampu Bintang, Safliah Rahmayani; Manalu, Kartika; Nasution, Rizki Amelia
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 5 No 3 (2024): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Peraturan Menteri Kesehatan RI NO.492/Menkes/Per/IV/2010 menyatakan bahwa air minum harus memenuhi kriteria tertentu agar dapat dianggap layak dikonsumsi.  Kriteria tersebut antara lain tingkat kontaminasi 0 koloni per 100 ml untuk keberadaan bakteri koliform dan bakteri Escherichia coli.  Tujuan dari penelitian ini adalah untuk mengisolasi dan mengkarakterisasi bakteri Escherichia coli yang terdapat pada sumber air minum di Desa Banjar Toba yang terletak di Kecamatan Berampu.  Berdasarkan hasil penelitian isolasi dan identifikasi, diketahui terdapat bakteri Escherichia coli pada 7 sampel, dengan metode isolasi bakteri menggunakan media Eosin Methylene Blue (EMB) dan uji Total Plate Count (TPC).  Kesimpulan ini didukung oleh hasil yang diperoleh dari pewarnaan gram.  Berdasarkan pengamatan kelimpahan bakteri Escherichia coli, ditentukan bahwa sampel MA 7 x 100 menunjukkan kelimpahan tertinggi, sedangkan sampel MA 4 20 x 100 menunjukkan kelimpahan terendah. Berdasarkan penelitian ditemukan bahwa sumber air minum di Desa Banjar Toba tidak memenuhi persyaratan yang telah ditentukan oleh Peraturan Menteri Kesehatan NO.492/Menkes/Per/IV/2010.
Isolasi dan Identifikasi Bakteri Brucella sp. pada Darah Kambing di Desa Air Joman Kecamatan Air Joman Kabupaten Asahan Sinaga, Sabariah; Manalu, Kartika; Nasution, Rizki Amelia
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 5 No 3 (2024): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v5i3.464

Abstract

Brucellosis adalah penyakit menular yang disebabkan oleh bakteri Brucella sp., yang dapat menginfeksi berbagai jenis hewan ternak dan manusia. Penyakit ini sering kali menimbulkan masalah serius dalam peternakan dan kesehatan masyarakat karena kemampuannya untuk menyebar melalui kontak dengan hewan yang terinfeksi atau produk-produk hewani yang terkontaminasi. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri Brucella sp. yang menjadi penyebab Brucellosis pada kambing. Metode yang digunakan mencakup isolasi bakteri menggunakan media TSA (Tryptic Soy Agar) dan uji RBT (Rose Bengal Test), serta analisis data penelitian dilakukan secara kualitatif. Hasil dari isolasi bakteri menunjukkan bahwa dua sampel dari jumlah total sampel darah kambing yang diperiksa menunjukkan hasil positif terhadap bakteri Brucella sp. dengan kode isolat KB1 dan SdJ2 dengan media TSA. Identifikasi dilakukan berdasarkan pertumbuhan koloni yang khas pada media kultur, serta analisis pewarnaan gram yang mengungkapkan karakteristik mikroorganisme di bawah mikroskop. Namun, hasil uji RBT menunjukkan hasil yang kurang memuaskan dalam mendeteksi Brucella sp., dengan tidak adanya penggumpalan dan ketidakkonsistenan warna ungu kemerahan pada serum. Perbedaan ini menyoroti tantangan dalam menggunakan metode tes tunggal untuk diagnosis yang akurat, karena adanya kemungkinan hasil negatif palsu atau rendahnya sensitivitas tes. Oleh karena itu, untuk memastikan diagnosis yang lebih akurat dan dapat diandalkan, disarankan untuk menggunakan lebih dari satu prosedur tes lanjutan, seperti ELISA (Enzyme-Linked Immunosorbent Assay) dan PCR (Polymerase Chain Reaction). Kombinasi metode ini dapat meningkatkan kepekaan dan spesifisitas diagnosa Brucellosis pada hewan ternak.
Co-Authors Adisty, Yuda Agusna, Sri Anas, Nirwana Arbaiyah, Seri Ayundari, Sella Bintang, Safliah Rahmayani Dwi Pratiwi, Anggrainy Fatimah Azzahra Fermani, Nuritia Fitri Amalia Ginting, Monika Habibunnisa, Habibunnisa Hasibuan, Ira Aqrabi Br Hasra, Barratun Nisa Herni, Zulfiana Hutasuhut, Melfa Aisyah Jayanti, Ummi Jayanti, Ummi Nur Afinni Dwi Julianty, Dwita Khadijah, Nur Khairuna Khairuna Kirana, Fira Maha, Destri Br Marsyah, Shafira Hana Najah, Nurun Nasution, Rizki Amelia Nst, Rizki Amelia Nurwijayanti Pasaribu, Nurul Alda Pulungan, Erlinda Marito Putri Lubis, Febrianti Rafika Annisa, Rafika Rahmadina, Rahmadina Raselia, Citra Raudhatul Jannah Riris Nurkholidah Rambe Rohani Rohani Sartika, Dea Simarmata, Faried Raja Sinaga, Sabariah Siregar, Abdul Hasym Mazhurin Siregar, Khoirul Bahria Siti Fatimah Sucahyo, Ahmad Imam Syarifah Widya Ulfa Tazkiah, Citra Utami, Mutiara Alya Zahratul Idami Zelila, Umma Selma Zuhra, Arini