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All Journal Meditory : The Journal of Medical Laboratory Jurnal Analis Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang JMLS: Journal of Medical Laboratory and Science Current Biomedicine Jurnal Kesehatan Siliwangi Jurnal Media Analis Kesehatan JIMMI: Jurnal Ilmiah Mahasiswa Multidisiplin
Durachim, Adang
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Religion Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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KORELASI KADAR FERRITIN DAN KADAR IL-6 TERHADAP SATURASI OKSIGEN PADA PASIEN COVID-19 DI RUMAH SAKIT ADVENT BANDUNG Tania, Monica; Nurhayati, Betty; Hayati, Eem; Durachim, Adang
Journal of Medical Laboratory and Science Vol 5 No 1 (2025): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/medlabscience.v5i1.2086

Abstract

Background: Covid-19 was first discovered in late 2019 in Wuhan, China caused by the coronavirus-2 or SARS-Cov-2. This virus causes a cytokine storm characterized by hyperferritinemia and increased IL-6 levels. Most Covid-19 patients require ventilator assistance due to severe pneumonia, alveoli damage, lung parenchyma damage, and the occurrence of Acute Respiratory Distress Syndrome (ARDS) characterized by decreased oxygen saturation. Objective: to find a correlation between Ferritin levels and IL-6 levels on oxygen saturation. Methods: The type of research used is descriptive. The study population and samples were all patients of Advent Hospital Bandung who were admitted to the ICU room infected with Covid-19 with a positive PCR value with a CT value <40 and examined for Ferritin, IL-6 and oxygen saturation parameters in the period January-December 2021. And using the Spearman correlation statistical test. Results: It is known that the average Ferritin level is 1,528.04 ng/mL, the average IL-6 level is 238.35 pg/mL and the average oxygen saturation value has an average value of 90.1%, and there is a correlation between Ferritin levels and oxygen saturation of 29.7%, but there is no correlation between IL-6 levels and oxygen saturation. Conclusion: there is a correlation between Ferritin levels and oxygen saturation which has a weak level of relationship. Suggestion: Ferritin is recommended as an initial examination to indicate the good and bad condition of Covid-19 patients and can help clinicians in better and faster management of Covid-19 patients, because there is a relationship with oxygen saturation values.
THE EFFECT OF EDTA BLOOD STORAGE TIME AND ROOM TEMPERATURE ON THE EXAMINATION OF ERYTHROCYTE SEDIMENTATION RATE Nabila, Lazulpa; Durachim, Adang; Hayati, Eem; Noviar, Ganjar
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3517

Abstract

Background: Erythrocyte Sedimentation Rate (ESR) is an examination that aims to determine the speed of erythrocytes settling in the blood measured for 1 hour. The examination of ESR performed within 2 hours at most. However, the examination is often not performed immediately due to several reasons that cause delays for several hours. The room temperature during the examination affects the examination. The temperature in West Java has the highest average reaching 32°C. Aims: To determine an effect of EDTA blood storage time and room temperature on the examination of ESR. Method: This research is included in the type of quasi eksperimental research by involving variations in blood storage time immediately, 4 hours, and 5 hours and room temperature during examination is room temperature 20-25oC and temperature 29-32oC. Result: The result of this study showed that there was a significant effect of the variable EDTA blood storage time and room temperature on the examination of ESR which is indicated by the value of Sig. 0,00 (Sig. 0,05). This is because EDTA blood stored more than 2 hours after blood collection will affect the shape of the cells and the temperature can affect the settling speed. Conclusion: Based on the results of statistical tests, it can be concluded that there is an effect of EDTA blood storage time and room temperature on the examination of ESR
PERBANDINGAN PEMERIKSAAN JUMLAH TROMBOSIT METODE MANUAL (AMONIUM OKSALAT) DAN METODE PENCITRAAN DIGITAL (IMAGEJ) PADA KAMAR HITUNG IMPROVED NEUBAUER Gusmana, Riki; Firman Solihat, Mohamad; Nurhayati, Betty; Durachim, Adang; Kristian, Erick
Jurnal Kesehatan Siliwangi Vol. 6 No. 2 (2025): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

ABSTRACT Platelet count examination is a crucial parameter in evaluating hemostatic function, especially in diagnosing diseases such as dengue fever. This study compares the manual method using Ammonium Oxalate reagent with the digital imaging method utilizing ImageJ software on the Improved Neubauer chamber. The research aims to evaluate the differences between these methods and compare them with the automated method (Hematology Analyzer) as the gold standard. The study employed an observational analytic design with a cross-sectional approach. Samples consisted of blood from patients undergoing complete hematology examinations at RSUD Bedas Kertasari, Bandung Regency. Results from the three methods were compared to determine accuracy, efficiency, and objectivity. The results of the study showed the quantitative distribution of platelet counts using automatic (Hematology Analyzer), manual (Ammonium oxalate) and digital imaging (ImageJ) tools obtained the average values ​​respectively: 302,400 cells/mm3, 297,500 cells/mm3, 305,000 cells/mm3. There was no statistically significant difference in the test of platelet count examination data pairs between the automatic method (Hematology Analyzer) and the manual method (Ammonium oxalate) and between the automatic method data pairs (Hematology Analyzer) and the digital imaging method (ImageJ). While in the test of platelet count examination data pairs in the Improved Neubauer counting chamber between the manual method (Ammonium oxalate) and the digital imaging method (ImageJ) there was a statistically significant difference, but clinically the difference was still within the permissible threshold.
Pengaruh Suhu dan Lama Simpan Washed Red Cell Terhadap Nilai Fragilitas Osmotik Metode Spektrofotometri A, Nisa; Nurhayati, Betty; Noviar, Ganjar; Durachim, Adang
Jurnal Media Analis Kesehatan Vol 16 No 2 (2025): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v16i2.1682

Abstract

Washed Red Cell adalah produk darah yang diperoleh dari proses pencucian Packed Red Cell, di mana zat aditifnya dihilangkan, sehingga lebih mudah mengalami hemolisis. Hemolisis saat penyimpanan bisa dipicu oleh faktor suhu dan lama penyimpanan. Penelitian ini ditujukan guna mengkaji pengaruh suhu serta lama penyimpanan terhadap tingkat fragilitas osmotik pada Washed Red Cell menggunakan metode spektrofotometri. Penelitian ini menggunakan rancangan kuasi-eksperimen, dengan satu unit kantong Washed Red Cell bergolongan darah O Rh+ yang ditempatkan pada kondisi suhu lemari pendingin dan suhu lingkungan selama 0, 4, 7, serta 10 jam. Hasil telaah deskriptif menunjukkan adanya kenaikan tingkat fragilitas osmotik pada seluruh kelompok perlakuan. Uji Friedman menunjukkan bahwa suhu penyimpanan refrigerator dengan ruang dan durasi penyimpanan (0, 4, 7, dan 10 jam) berpengaruh signifikan pada nilai fragilitas osmotik, dengan nilai signifikansi kurang dari 0,001 (P<0,05). Uji Wilcoxon juga mengonfirmasi adanya pengaruh lama penyimpanan, di mana sampel yang disimpan segera berbeda signifikan dengan yang ditunda selama 4, 7, dan 10 jam, dengan nilai signifikansi tidak melebihi 0,001 (P<0,05). Selain itu, uji Spearman menunjukkan hubungan yang kuat antara suhu penyimpanan dan lama penyimpanan dengan nilai fragilitas osmotik, dengan nilai signifikansi kurang dari 0,001 (P<0,05). Hubungan ini bersifat positif, ini berarti makin tingginya suhu dan makin lamanya waktu penyimpanan, maka nilai fragilitas osmotik pun akan semakin meningkat.
Co-Authors A, Nisa Arum, Nafilah Fathiasari, Fariha Firman Solihat, Mohamad Fitriani, Ishma Dwi Gusmana, Riki Gustira Rahayu, Ira Hardi, Zon Hayati, Eem Juliastuti, Aditya Kristian, Erick Lamsudiansyah, Asrul Yudha Fadhiila Mamat Rahmat Marliana , Nina Mufidah, Zahra Nabila, Lazulpa Nina Marliana, Nina Novia, Ganjar Noviar, Ganjar Nurdianti, Dinda Nurhayati, Betty Riyani, Ani Solihat, Mohamad Firman Suhartini, Anita Tania, Monica Widiawati, Widi Wiryanti, Wiwin