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All Journal HAYATI Journal of Biosciences AL KAUNIYAH Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Canrea Journal: Food Technology, Nutritions, and Culinary Journal Jurnal Pengabdian Masyarakat: Darma Bakti Teuku Umar Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education
Eko Agus Suyono
Faculty Of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Kampus UGM, Yogyakarta 55281, Indonesia; Center Of Excellence For Microalgae Biorefinery, Universitas Gadjah Mada, Sekip K1A, Yogyakarta 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : HAYATI Journal of Biosciences

 1 
Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis Achmad Rodiansyah; Ainul Fitria Mahmudah; Mastika Marisahani Ulfah; Uun Rohmawati; Dwi Listyorini; Eko Agus Suyono; Sitoresmi Prabaningtyas
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.136

Abstract

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.
Effects of Copper on Euglena sp. Local Strains and Remediation Capabilities Ardipeni, Witia; Suyono, Eko Agus; Dewayanto, Nugroho; Mufida, Na’imatul; Erfianti, Tia; Amelia, Ria; Kurnianto, Dedy
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.387-394

Abstract

One of the well-known industries in Yogyakarta is the Kotagede silver craft, which produces heavy metal waste, one of which is copper. The copper content in the liquid waste of electroplating Kotagede silver crafts is 4.628 mg/L. At the same time, Indonesian Government Regulation No. 22 (2021) stipulates a critical limit of Copper (Cu) content in river and lake water, which is 0.2 mg/L. The purpose of the study was to analyze the effects of copper on local strains of Euglena sp. (growth, photosynthetic pigment production, and superoxide dismutase enzyme) as well as the effectiveness of Euglena sp. in copper remediation. The study was conducted by adding a copper solution obtained from CuSO4•5H2O to the culture medium of Euglena sp. The parameters observed in the study were growth (number of cells and biomass), photosynthetic pigment content (chlorophyll a, chlorophyll b, carotenoid total), Superoxide dismutase enzyme of Euglena sp. as well as the effectiveness of Euglena sp. in copper remediation. The results showed that copper inhibited the growth of Euglena sp. The highest growth was the control treatment (without adding copper to the culture medium). Likewise, the content of photosynthetic pigments and the culture of Euglena sp. exposed to copper were lower when compared to the control culture. The activity of the SOD enzyme is increased in cultures exposed to copper. The result of the research is Cu metal absorption efficiency by Euglena sp. by 21.93%, 10.93%, and 9.47% for a Cu concentration of 10 ppm, 15 ppm, and 20 ppm.
Exploration of Lipid Profile and Wax Ester Content from Local Strain Euglena sp. IDN33 Cultivated in Mixotrophic Condition Combined with Molasses Supplementation Astiti, Adam; Erfianti, Tia; Maghfiroh, Khusnul Qonita; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.805-818

Abstract

The transition from fossil fuels to sustainable renewable energy is currently growing rapidly. Euglena sp. can be the source of biofuel. Molasses, in addition to Euglena sp., can increase the levels of paramylon and wax esters produced. Therefore, this study aims to analyze cell growth, lipid profile, and wax ester content of Euglena sp. cultivated under mixotroph conditions with molasses. Euglena was cultivated under mixotrophic conditions with the addition of various concentrations of molasses (0, 10, 15, and 20 g/L) until the final log phase. Then, measurements were made on cell density, levels of lipids, proteins, and paramylon to measure the growth and metabolites profile of Euglena sp., along with lipid profile and wax ester analysis using GC-MS. The results of the study showed that 10 g/L molasses increased Euglena growth (SGR 0.1736± 0.0213 mg/ml) and carbohydrate (0.426 mg/ml) but reduced lipid accumulation (2.29 mg/ml). This treatment provides the best result before control. Molasses does not increase carbohydrates and protein but increases paramylon accumulation. The GC-MS assay detected more lipid profiles and concentrations of each lipid type in non-molasses-treated Euglena sp. compared to molasses-treated. Euglena was cultivated on molasses, which was dominated by palmitic acid and myristyl myristate with the absence of PUFA-type fatty acids in the cells, while the untreated ones were dominated by stearic acid and myristyl myristate. Thus, 10 g/L supplementation of molasses is the most effective treatment compared to others.
Co-Authors Achmad Rodiansyah Adhy Kurniawan Afifah, Marshanda Nur Roosyana Aini, Hasna Nur Ainul Fitria Mahmudah Amalia Rahmawati Amelia, Ria Ardipeni, Witia Arief Budiman Asmi Citra Malina Astiti, Adam Baihaqi, Yahya Baroqah, Nuzul Budi Kamulyan BUDI SETIADI DARYONO Dwi Listyorini Dwi Umi Siswanti Eko Nurcahya Dewi Erfianti, Tia Hardianto, Muhammad Andhi Herri Darsan Himawan Tri Bayu Murti Petrus Intan Supraba, Intan Irja, Muhammad Irma Rohmawati Karilanata, Khalid Erlangga Karillanata, Khalid Erlangga Kurnianto, Dedy Laksmindra Fitria Larasati, Ersi Lucia Tri Suwanti, Lucia Tri Lukita Purnamayati Luthfiana, Dwi Hardianti M Iqbal Maulana Ginting Maghfiroh, Khusnul Qonita Mahendra, Himawan Akbar Mastika Marisahani Ulfah Mochammad Donny Koerniawan Mochammad Yuwono Mufida, Na’imatul Muhammad Haikal Mulyati Sarto Nugroho Dewayanto, Nugroho Nurafifah, Istini Nurcahyo, Raden Wisnu Putri, Renata Adaranyssa Egistha Rahadian Yudo Rasdi, Abdurrahman Muhammad Fikri Rohmawati, Uun Rudi Hermi Sadewo, Brilian Ryan Satriayudistira, Jody Ashrib siti mudrikah Sitoresmi Prabaningtyas Slamet Widiyanto Solly Aryza Tantri, Dea Hastaning Tia, Erfianti Tri Wahyu Setyaningrum, Tri Wahyu Ulfah Juniarti Siregar Wardana, Wisnu Eka Yuliatul Muslimah