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Julisaniah, Nur Indah
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Characterization and Identification of Lactic Acid Bacteria (LAB) in Spontanoiusly Fermented Arabika (Coffea arabica L.) Fruits on Lombok Island Muspiah, Aida; Nurrijawati, Nurrijawati; Julisaniah, Nur Indah; Farista, Baiq
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10512

Abstract

The quality of arabica coffee (Coffea arabica, L) is not only influenced by the quality of the coffee beans, but also by the distinctive flavor that is the main attraction for coffee lovers. Various coffee post-harvest processing methods have been developed, including fermentation. The presence of lactic acid bacteria (LAB) that important in the spontaneous fermentation, cause it can be influenced by the geographical location of arabica coffee plants. Currently, information on the presence of LAB in arabica coffee on Lombok Island has not been available. Therefore, it is important to identify the LAB involved in the spontaneous fermentation of Arabica coffee. This study aims to identify the species of LAB in arabica coffee on Lombok Island by the spontaneous fermentation method. Characterization and identification of bacteria were carried out using phenotypic identification and profile matching methods, which include several observation parameters, colony morphology and cell morphology, physiological tests (bacterial growth at certain pH and temperature), and biochemical tests such as catalase test and carbon source usage. In this study, nine pure isolates that belong to the LAB group were successfully isolated. Based on the identification results, LAB belonged to the genus Lactobacillus. The isolates identified consisted of one isolate of Lactobacillus plantarum (IBK1), one isolate of Lactobacillus sakei (IBK3), and seven other isolates identified as Lactobacillus sp (IBK2, IBK4, IBK5, IBK6, IBK7, IBK8, IBK9). These findings can be the first step in the development of starter cultures for controlled fermentation in Arabica coffee.  
Diversity of Durian (Durio zibethinus Murr) in Aik Bual Village, Central Lombok Regency Aryanti, Evy; Sukenti, Kurniasih; Julisaniah, Nur Indah; Muspiah, Aida; Sukiman, Sukiman; Thalita, Deandra Alma
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11359

Abstract

Aik Bual Village has a durian community that grows durian, both local and introduced durian. The purpose of this study was to obtain information on the diversity and level of similarity of durian accessions in Aik Bual village, Central Lombok Regency in an effort to save and develop durian germplasm (Durio zibethinus) to obtain the diversity and level of similarity of durian accessions (D. zibethinus) in Aik Bual village, Central Lombok Regency. The study was conducted from December 2023 to October 2024. Sampling of D. zibenthinus accessions was collected using the exploration method. Morphological characterization data was analyzed using NTSys pc. 2.02i software. Durio kutejensis known as Lai was used as an outgroup in this study. The results of the study found eight accessions of D. zibethinus, consisting of introduced durian accessions such as Musang King, Black Thorn (Ochee), Montong, Kane, and Otong, while local durian accessions such as Mentega, Susu, and Sukun. Kinship analysis with NTsys resulted in two main groups, the first group consisting of Montong, Kane and Otong while the second group consisted of Musang King, Ochee, Sukun, Mentega and Susu.
Micropropagation of Wooden Banana (Musa paradisiaca L. cv. Kayu) Through In Vitro Sustiana, Maulia; Muspi'ah, Aida; Julisaniah, Nur Indah
JRST (Jurnal Riset Sains dan Teknologi) Volume 10 No. 1, March 2026: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v10i1.27457

Abstract

Wooden banana (Musa paradisiaca L. cv. Kayu) is a local banana variety that had unique characteristics, including a sweet taste, fragrant aroma, thicker skin, longer shelf life, and high economic value. This banana variety had a long growth period, requiring approximately 9 to 11 months to flower and bear fruit. Additionally, a single plant usually produced only one to three suckers, making wooden banana seedlings rare and difficult to obtain. To prevent its potential extinction, seedling propagation was necessary, with one possible solution being tissue culture techniques.   This study aimed to determine the optimal concentration of Benzyl Amino Purine (BAP) and the most effective culture medium for the growth of wooden banana shoots. The research employed an experimental method, testing various BAP concentrations (1 ppm, 2 ppm, 3 ppm, 4 ppm, and 5 ppm) on solid and semi-solid culture media. Data were analyzed descriptively based on the number of shoots produced under each treatment. The results showed that concentration of 3 ppm Benzyl Amino Purine (BAP) produced the highest shoot proliferation. The solid-liquid medium was the most effective culture medium, which produced an average of 4.33 shoots per explant. These findings indicate that appropriate BAP concentration and culture medium significantly enhance in vitro micropropagation of wooden banana.
In Vitro Multiplication of the Ornamental Plant Sente (Alocasia melo) Muspiah, Aida; Badriansyah; Julisaniah, Nur Indah
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.11722

Abstract

The genus Alocasia showed high diversity, including morphological differences among approximately 60 species. One of these species was Alocasia melo, which was distributed on Borneo Island. A. melo had high aesthetic and economic value, making it popular among ornamental plant enthusiasts. However, growing A. melo naturally was challenging due to specific habitat, such as humidity, temperature, lighting, water supply, soil pH, and indoor storage to increase survival rates. To address this, tissue culture (in vitro) was used as a method for its propagation and conservation.  This study aimed to determine the effects of different nutrient components on the in vitro growth of A. melo and to find the most effective nutrient combination for its growth. The research was an experimental study with treatments including coconut water + 6-Benzylaminopurine (BAP), algae extract + BAP, coconut water, algae extract, and coconut water + algae extract on solid media. The data were analyzed descriptively, showing the number of shoots and roots in tables and figures. The results showed that the treatment with coconut water (10 mL) + BAP (1 ppm) produced the highest average number of shoots (3.75). Meanwhile, the treatment with coconut water (30 mL) resulted in the highest average number of roots (6.75).
Co-Authors Adhawati, Laela AIDA MUSPIAH Badriansyah Bakti, Lalu Arifin Aria ERNIN HIDAYATI Evy Aryanti Farista, Baiq Faroby, Muhammad Azdy Hamidia, Baiq Zulifa Handayani, Agustia Fitri Haqiqi, Nirwana Ibadurahman, Lalu Faqih Indriana Manik Kaswari Ito, Michiho Kurniasih Sukenti Kurniasih Sukenti Layyina, Naviri Mamika Ujianita Romdhini, Mamika Ujianita Muspi'ah, Aida Nadia, Ahsanu Nur Indah Julisaniah Nurrijawati, Nurrijawati Permatasari, Baiq Dewi Pradita, I Made Nanda Prasedya, Eka Sunarwidhi Prazali, Arianteza Rahayu, Rachmawati Noviana RINA KURNIANINGSIH Salman Alrasyid, Salman Alrasyid Sukiman Sukiman Sukiman Sunarwidhi , Anggit Listyacahyani Sunarwidhi, Anggit Listyacahyani Supiatin Sustiana, Maulia Takamatsu, Sakura Thalita, Deandra Alma Tri Mulyaningsih Tri Wahyu Setyaningrum, Tri Wahyu Virgota, Arben Widiyanti, Astrini Yanagisawa, Masayuki Yumi, Fujiwara