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Syamsul Bahri
Fakultas Pertanian Universitas Samudra
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Eksplorasi Bakteri Endofit Terhadap Cendawan Pestalotiopsis microspora Penyebab Penyakit Gugur Daun Pada Tanaman Karet (Hevea brasiliensis Muell. Arg.) Vazza Navtra Tylova; Syamsul Bahri; Boy Riza Juanda; Alchemi Putri Juliantika Kusdiana
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 25 No 1 (2023)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.25.1.51-58

Abstract

[The Potential of Endophytic Bacteria as a Biological Controller of Leaf Fall Disease Caused by Pestalotiopsis sp. IN Rubber Plants (Hevea brasiliensis Muell. Arg.]. This study aims to explore endophytic bacteria and determine their effectiveness against the fungus Pestalotiopsis sp. which causes leaf fall on rubber plants. Pestalotiopsis sp. can attack all rubber clones resulting in decreased latex production. The presence of pathogen attacks can result in enormous economic losses. Therefore, it is very important to find an effective and efficient controlling method, one of which is through biological control of plant diseases, namely by using antagonistic microorganisms. The presence of endophytic bacteria as biological controllers has become an alternative method to reduce the practices of chemical control. This study used a Non-Factorial Completely Randomized Design (CRD) with 6 treatments and 4 replications, so that 24 experimental units were obtained, using Potato Dextrose Agar (PDA) media for the in vitro antagonist test using the dual culture method. Namely P₀ = Control, P₁ = Pestalotiopsis sp. 1 vs Bacteria P, P₂ = Pestalotiopsis sp. 2 vs Bacteria B, P₃ = Pestalotiopsis sp. 3 vs Bacteria C, P₄ = Pestalotiopsis sp. 4 vs Bacteria E, P₅ = Pestalotiopsis sp. 5 vs Bacteria F. The results indicated that antagonistic treatment of endophytic bacteria in vitro affected the percentage of inhibitory power of the mycelium of the fungus Pestalotiopsis sp. by endophytic bacteria at 1 - 6 Days After Incubation (DAI). The 3% KOH method and gram staining showed that samples P₁, P₂, P₅ did not produce mucus and were purple in colour (positive), but samples P₃, P₄, showed mucus and were pink in colour (negative). Hypersensitivity reactions to tobacco plants samples P₁, P₂, P₃, P₄, P₅ did not show negative characteristics or did not cause changes in coloration and symptoms on leaves of tobacco plant.  
Co-Authors Alchemi Putri Juliantika Kusdiana Amal Saber Abdel-Rahman Andika Putriningtias arif, zainal - Basri, Teuku Hasan Boy Riza Juanda Cici Indriani Dalimunthe Cut Mulyani Cut Mulyani Esraa Elsaeed Ibrahim Ammar Fara Nadilla Fara Nadilla Farasmayuda Fazri Amir Fitriani Fitriani Fitriani Fitriani Fitriani Fitriani Helmi Akbar Helmy Akbar Husna Maulida Iskandar Iskandar Iswahyudi Iswahyudi Iswahyudi, Iswahyudi Komariyah, Siti Muhammad Amin Muhammad Arif Nasution Muhammad Fauzan Isma Muhammad Syahril Muhammad Zulfri Mutiara Titik Falahiyah Nazaruddin Abdul Rachman Putriningtias, Andika Rahmatsyah Rahmatsyah Rhido Suhada Rozalina Rozalina Salman Alfarizi Saukani Saukani Siregar, Dolly Sojuangan Siti Komariah Teuku Fadlon Haser Teuku Muhammad Faisal Teuku Muhammad Faisal Teuku Muhammad Faisal Faisal Vazza Navtra Tylova Yenni Marnita Yulida Amri Yulida Amri