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PENGARUH ZOOXANTHELLAE KARAUG TERHADAP KELANGSUNGAN HIDUP DAN PERTUMBUHAN KERANG RAKSASA KIMA (TRIDACNA SQUAMOSA) Ambariyanto, Ambariyanto
JURNAL BIOLOGI INDONESIA Vol 4, No 3 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i3.3256

ABSTRACTThe influence of zooxanthellae isolated from corals on the growth and suwivorshipof giant clams. Giant clam known as marine bivalves (Tridacnidae) which live in coralreef. One important aspect of giant clam biology is the existence of zooxanthellae assymbiotic algae which have important role source of energy In hatchery operationalprocedure zooxanthellae were introduced into larvae. Zooxanthellae were isolated fromadult clams. Since clams are also known as endangered species, it is important to findother sources of zooxanthellae. The objective of this research is to investigate the effectof introduction of zooxanthellae which were isolated from different sources on the survivaland growth of giant clams larvae. The results showed that there is no differences on thesurvivorship and growth of giant clams larvae introduced by zooxanthellae isolated fromseveral corals. This result has open a possibility of using corals as source of zooxanthellaeinstead of using giant clams.Key words : zooxanthellae, giant clams, survivorship, growth

Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria Sedjati, Sri; Ambariyanto, Ambariyanto; Trianto, Agus; Supriyantini, Endang; Ridlo, Ali; Bahry, Muhammad Syaifudien; Wismayanti, Gita; Radjasa, ocky Karna; McCauley, Erin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.438

This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.Â Meanwhile, theÂ ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria

Heavy Metal Contamination on Vannamei Shrimp Aquaculture in North Coast of Central Java Apresia, Fadil; Ambariyanto, Ambariyanto; Yulianto, Bambang; Payus, Carolyn Melissa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 3 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.3.241-250

The North Coast of Central Java receives consignments of waste containing heavy metal contamination from several sources, resulting in a decrease in water quality on the coast. Heavy metal contamination in water sources, such as Pb, Cd, and Cr that will be used in shrimp farming. The impact of Pb, Cd, and Cr can have several adverse effects, such as health risk, ecological imbalance, reduce shrimp growth and productivity, environment pollution. The research was conducted to analyze heavy metal contamination levels in ponds and vannamei shrimp yields on the northern coast of Central Java. The method involved collecting shrimp, water, and sediment samples at nine different locations (Dampyak; Suradadi; Kedongkelor; Danasari; Nyamplungsari; Pesantren; Depok; Wonokerto; Degayu). Heavy metal data were analyzed with SPSS. The mean lead concentrations were 0.52+0.19 mg.kg-1 in sediment, 0.66+0.11 mg.kg-1 in water, and 0.86+0.18 mg.kg-1 in shrimp. Cadmium levels were measured in soil (0.028+0.03 mg.kg-1), water (0.027+0.021 mg.kg-1), and shrimp (0.011+0.004 mg.kg-1). The mean chromium concentrations were 0.51+0.25 mg.kg-1 in sediment, 0.93+0.40 mg.kg-1 in water, and 0.95+0.11 mg.kg-1 in shrimp. Based on the calculation of the average BAF x > 1.0, vannamei shrimp on the northern coast of Central Java have the capability of becoming bioaccumulation and bioindicators. The consumption of vannamei shrimp for 70 years has a lifetime risk of developing cancer due to the LCR value of x > 10-6 on the northern coast of Central Java.

DNA Barcoding of Anchovy in Tuban Regency as Database of Indonesian Marine Genetic Diversity Joesidawati, Marita Ika; Nursalim, Nining; Kholilah, Nenik; Kurniasih, Eka Maya; Cahyani, Ni Kadek Dita; Ambariyanto, Ambariyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 4 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.4.383-391

Anchovy is the main catch and the primary consumption of coastal communities in Indonesia, and its production shows an increase of more than 10% in 2021. Tuban district, in East Java, Indonesia is part of the WPP 712 (Wilayah Pengelolaan Perikanan or Fisheries Management Area) and highly produces anchovies’ fisheries. Anchovy has a small size, making it difficult to identify morphologically. This study aimed to genetically identify anchovy samples obtained from North Java (Tuban) waters. Molecular identification was conducted by utilizing Cytochrome Oxidase Subunit I (COI) gene using jg-HCO and jg-LCO primers. This study observed 12 individual samples with 623 base pair sequence length. Five species were obtained, namely four species of anchovies (Encrasicholina heteroloba, Encrasicholina punctifer, Stolephorus waitei, and Stolephorus insularis) and one species of sardines (Dussumieria elopsoides) with 99.84-100% similarity to NCBI sequences data. Anchovies typically have a streamlined body with a slightly compressed shape. Anchovies have cycloid scales, which are smooth-edged and relatively small, ranging from a few centimeters to around 20 centimeters in length. Some of the genus from the Anchovy group are Encrasicholina and Stolephorus. The phylogenetic tree reconstruction leads into four clades with a genetic distance between clades of 17,9-24,5 %. This research provides methods and data on the genetic diversity of anchovies taxa caught in Tuban, East Java. The findings are expected to support promoting new standards for healthier and more sustainable anchovy stocks in the country. Overall, this study contributes to providing valuable insights for fisheries management and conservation efforts in Indonesia.

Mangrove Conservation and Biodiversity Protection Strategies in Universitas Diponegoro to Achieve Net Zero Emission Ambariyanto, Ambariyanto; Utama, Yos Johan; Sugianto, Denny Nugroho; Ariyanti, Dessy; Handayani, Elinna Putri
Journal of Sustainability Perspectives Vol 3, No 2: December 2023
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jsp.2023.20482

Indonesia is set the net zero emission target to be achieved by 2060. As part of that, Universitas Diponegoro (UNDIP) is committed to support the achievement of the goal by implementing several strategies. First is to reduce the amount of emission generated by the university daily activities, and second is boosting the activities of mangrove conservation and biodiversity protection. As UNDIP located in the northern part of Central Java, part of 108.000 kilometers coastline region of Indonesia, UNDIP prioritizes its academic activity and research focus on coastal region environmental and ecological development which enthuse the implementation of mangrove conservation and biodiversity protection. The strategy includes the structured mangrove and biodiversity conservation action directed by the University leaders, encouraging research and community service activities focusing on coastal region development and developing environmental-related curriculum for the students. In addition, UNDIP also supports research centers and students’ communities who focus on mangrove conservation, biodiversity protection, and coastal region development. By that, there are more than 50 types of flora in UNDIP land forest with carbon stock calculation up to 6,480.20 metric tons CO2 eq. Acc

Feeding rats with used cooking oil elevates malondialdehyde, TNF-α, and creatinine compared to tempe fried with used oil Murwani, Retno; Susilaningsih, Neni; Ariyanto, Diaza O.; Ambariyanto, Ambariyanto
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.853

In vivo studies on the hazards of deep-fried foods were commonly done by feeding used- or heated-cooking oil to rats. The aim of this study was to determine the effect of feeding tempe deep-fried in palm, olive, and coconut oils and the used frying oil on the blood biochemical profile of laboratory rats. An in vivo randomized control group study with pre-test and post-test was conducted. This study included healthy male Sprague-Dawley rats aged 2–3 months and weighing 100−200 grams. After acclimatization, the rats were randomly assigned to seven groups, which were: (1) regular diet (control diet); (2) diet of tempe deep-fried in 5× used palm oil (Tempe-in-used-Po); (3) diet of tempe deep-fried in 5× used coconut oil (Tempe-in-used-Co); (4) diet of tempe deep-fried in 5× used olive oil (Tempe-in-used-Oo); (5) diet of 5× used palm oil (Used-Po); (6) diet of 5× used coconut oil (Used-Co); and (7) diet of 5× used olive oil (Used-Oo). Each rat received 15 grams of a treatment diet daily and blood samples were collected after four weeks for a complete blood count and serum biochemistry analysis. The results showed that the final body weight and the weight gain of Tempe-in-used-Po, Tempe-in-used-Co, Tempe-in-used-Oo group, and Used-Po groups increased significantly compared to the control, Used-Co, and Used-Oo groups. However, there was a significant increase in serum tumor necrosis factor-alpha (TNF-α) in the Used-Co and Used-Oo groups (p<0.05), suggesting the used oil's detrimental effect. The Used-Co and Used-Oo were the only two groups whose creatinine increased significantly (p<0.05). Subsequently, only the Used-Oo group had a significantly increased malondialdehyde (MDA) level compared to all groups (p<0.05). These results prove that the effect of feeding fried food differs from used oils. Feeding used oil did not reflect the consumption of fried foods as part of the whole diet and generally resulted in more harmful effects. This is the first study to report an in vivo rat feeding study of deep-fried tempe and the used oil as part of the diet.

Mangrove Conservation and Biodiversity Protection Strategies in Universitas Diponegoro to Achieve Net Zero Emission Ambariyanto, Ambariyanto; Utama, Yos Johan; Sugianto, Denny Nugroho; Ariyanti, Dessy; Handayani, Elinna Putri
Journal of Sustainability Perspectives Vol 3, No 2: December 2023
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jsp.2023.20482

Indonesia is set the net zero emission target to be achieved by 2060. As part of that, Universitas Diponegoro (UNDIP) is committed to support the achievement of the goal by implementing several strategies. First is to reduce the amount of emission generated by the university daily activities, and second is boosting the activities of mangrove conservation and biodiversity protection. As UNDIP located in the northern part of Central Java, part of 108.000 kilometers coastline region of Indonesia, UNDIP prioritizes its academic activity and research focus on coastal region environmental and ecological development which enthuse the implementation of mangrove conservation and biodiversity protection. The strategy includes the structured mangrove and biodiversity conservation action directed by the University leaders, encouraging research and community service activities focusing on coastal region development and developing environmental-related curriculum for the students. In addition, UNDIP also supports research centers and students’ communities who focus on mangrove conservation, biodiversity protection, and coastal region development. By that, there are more than 50 types of flora in UNDIP land forest with carbon stock calculation up to 6,480.20 metric tons CO2 eq. Acc

Pengelolaan Desa Wisata Gronjong Wariti Dengan Konsep Community-Based Tourism Arsana, Arsana; Ambariyanto, Ambariyanto
Buletin Ekonomika Pembangunan Vol 2, No 2 (2021): SEPTEMBER
Publisher : Jurusan Ilmu Ekonomi Fakultas Ekonomi Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/bep.v2i2.13868

Penelitian ini bertujuan untuk mengetahui model penerapan dalam pengelolaan desa wisata Gronjong Wariti dengan konsep Community Based Tourism. Metode penelitian yang digunakan dalam penelitian ini adalah deskriptif kualitatif. Jenis data yang digunakan yaitu data primer dan data sekunder. Data primer dalam penelitian ini diperoleh dari dari wawancara langsung terhadap Pengelola Wisata Gronjong Wariti, Kepala Desa Mejono, masyarakat, dan pengunjung, serta dokumentasi. Data sekunder dalam penelitian ini diperoleh dari situs resmi Dinas Pemberdayaan masyarakat dan Desa, serta lembaga Badan Usaha Milik Desa Hapsari. Teknik analisis data meliputi pengumpulan data, reduksi data, penyajian data, dan penarikan kesimpulan. Uji keabsahan data menggunakan triangulasi sumber.Hasil penelitian ini menunjukkan bahwa pengelolaan Desa Wisata Gronjong Wariti menggunakan pengelolaan pariwisata berbasis masyarakat dengan sistem manajemen terbuka. Kendala yang dihadapi dalam pengelolaan Wisata Gronjong Wariti masih rendahnya Sumber Daya Manusia, partisipasi masyarakat secara luas kurang maksimal yang dilatarbelakangi adanya rasa kurang percaya dan kecurigaan masyarakat dalam pengelolaan Wisata Gronjong Wariti. Pemberdayaan masyarakat yang dilakukan berdampak pada partisipasi masyarakat dalam pengelolaan wisata. Wisata Gronjong Wariti juga berkontribusi terhadap Desa Mejono berupa Pendapatan Asli Desa dan peningkatan pendapatan masyarakat lokal.Kata Kunci: Pariwisata, Partisipasi, Pemberdayaan, dan Community-Based Tourism

Circular Economy Approach for Sustainable Tree Litters Waste Management, study case in Universitas Diponegoro Ariyanti, Dessy; Ambariyanto, Ambariyanto; Utama, Yos Johan; Sugianto, Denny Nugroho; Hapsari, Farida Diyah
Journal of Sustainability Perspectives Vol 4, No 2: December 2024
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jsp.2024.24809

Universitas Diponegoro, with an open forest area and planted vegetation of 30 – 40%, produced vast amounts of organic waste, especially tree litter. Piles of tree litter are everywhere if it is not properly managed. Universitas Diponegoro has taken a comprehensive approach to handling leaf and tree litter by converting it into economically valuable and beneficial products to meet the objectives of SDGs 12, which are focused on Responsible Consumption and Production. Through the Technical Implementation Unit for Occupational Safety, Health, and Environment (UPT K3L) Universitas Diponegoro has pyrolyzed tree branches to create liquid smoke and anaerobically converted leaf waste into compost. Later, the liquid smoke produced by pyrolysis and compost can achieve the consumer demand standard. In addition, from the policy approach, it is mandatory for every building to handle tree litter surrounds into compost using composting pits and composting bags. The circular economy idea has been incorporated into waste management at Universitas Diponegoro.

Pengaruh Pertumbuhan Ekonomi, Rata-Rata Lama Sekolah Dan Upah Riil Terhadap Penyerapan Buruh Masrury, Muhammad Mahdi; Affin, Rifa'i; Ambariyanto, Ambariyanto
Neo-Bis Vol 12, No 2 (2023): DESEMBER
Publisher : Trunojoyo University of Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/nbs.v12i2.28831

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