Article Per Year (5 Year)
p-Index From 2020 - 2025
2.56
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Makara Journal of Science
Abdjad Asih Nawangsih
Department Of Plant Protections, Faculty Of Agriculture, Institut Pertanian Bogor, Jalan Agatis Kampus Dramaga Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

Published : 32 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 14 Documents
Search
Journal : Jurnal Fitopatologi Indonesia

 1   2 
Character Diversity of Black Rot Bacterial Strains (Xanthomonas campestris pv. campestris) on Cabbage against Mixture of Active Ingredients Azoxystrobin and Diphenoconazole Af'idzatuttama; Nawangsih, Abdjad Asih; Giyanto
Jurnal Fitopatologi Indonesia Vol 19 No 2 (2023): Maret 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.2.45-56

Abstract

Character Diversity of Black Rot Bacterial Strains (Xanthomonas campestris pv. campestris) on Cabbage against Mixture of Active Ingredients Azoxystrobin and Diphenoconazole Xanthomonas campestris pv. campestris is a bacterium that causes black rot on cabbage. Farmers still rely on the synthetic pesticides azoxystrobin and difenoconazole to control diseases in cabbage which are feared to have an impact on the diversity of X. campestris pv. campestris. The objective of the research was to obtain genetic and phenotypic diversity data on X. campestris pv. campestris, as well as obtaining data on the resistance response of these bacterial isolates to a mixture of the active ingredients azoxystrobin and difenoconazole. The study consisted of five stages (1) sampling on land with black rot symptoms; (2) isolation of bacteria using tissue implant technique; (3) selection of isolates by testing properties of Gram, hypersensitivity, starch hydrolysis, and pathogenicity; (4) identification using specific and universal 16S rRNA primers; and (5) analysis of genotypic diversity by in silico RFLP method and phenotypic diversity by measuring EPS weight. The results of molecular characterization and identification obtained five isolates of X. campestris pv. campestris (CLT01, CDA08, SDA02, SDA22, and SDA26). The results of the analysis of genotypic diversity showed that the five isolates had genetic diversity based on the cutting of the 16S rRNA gene DNA fragment, while phenotypically indicated different Inhibition concentration (IC) values. SDA22 isolate had the highest IC50 value and different genetic diversity compared to other X. campestris pv. campestris. The use of synthetic pesticides azoxystrobin and difenoconazole continuously for a long time is feared to have an impact on pathogenic microbes such as X. campestris pv. campestris. So that other control alternatives are needed so that there is no resistance to these pathogenic microbes.
Streptomyces spp. as Biocontrol Agents of Fusarium Basal Rot on Shallots: Streptomyces spp. as Biocontrol Agents of Fusarium Basal Rot on Shallots Eka Wijayanti; Asih Nawangsih, Abdjad; Toding Tondok, Efi
Jurnal Fitopatologi Indonesia Vol. 20 No. 2 (2024): Maret 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.2.57-65

Abstract

Streptomyces spp. telah banyak diteliti sebagai agens pengendali hayati penyakit tanaman karena kemampuannya dalam menghasilkan berbagai jenis antibiotik, terutama senyawa-senyawa anticendawan. Aplikasinya untuk pengendalian Fusarium oxysporum f. sp. cepae penyebab penyakit busuk fusarium pada bawang merah di Indonesia belum dieksplorasi. Penelitian ini bertujuan mengevaluasi efektivitas Streptomyces spp. dalam mengendalikan F. oxysporum f. sp. cepae serta senyawa apa saja yang dihasilkan. Pengujian yang dilakukan meliputi uji efektivitas Streptomyces spp. terhadap pertumbuhan bawang merah dan insidensi penyakit busuk fusarium, serta analisis senyawa yang dihasilkan oleh Streptomyces spp. Hasil pengujian menunjukkan bahwa Streptomyces spp. tidak dapat meningkatkan parameter pertumbuhan. Sementara itu, pemberian Streptomyces spp. mampu menekan insidensi penyakit busuk fusarium dan memperpanjang masa inkubasi patogen. Analisis ekstrak kasar S. lydicus ABF 59 menunjukkan terdapat lima senyawa dominan yang bersifat anticendawan yaitu, d-limonene, tridecane, o-cymene, 2,4-di-tert-butylphenol (2,4-DTBP), dan hexadecanoic acid, methyl ester.
Identifikasi Patotipe Xanthomonas oryzae pv. oryzae dari Tanaman Padi di Sulawesi Selatan Asysyuura, Asysyuura; Nawangsih, Abdjad Asih; Mutaqin, Kikin Hamzah; Sudir, Sudir
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.562 KB) | DOI: 10.14692/jfi.13.3.73

Abstract

Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BLB), one of important constraint in rice production. The pathogen is known to have many pathotypes which caused difficulties in disease control. This research was conducted to determine the distribution of X. oryzae pv. oryzae’s pathotypes in seven districts in South Sulawesi. Grouping of pathotypes was performed according to Kozaka method, i.e. based on the response of differential rice varieties. Infection of X. oryzae pv. oryzae was confirmed by PCR using specific primers XOR-R2/XOR-F.  Out of 36 isolates, 29 isolates were identified as X. oryzae pv. oryzae which belong to pathotype III (6 isolates), pathotype IV (21 isolates), and pathotype XII (2 isolates).  This result indicated that X. oryzae pv. oryzae pathotype IV was distributed widely in South Sulawesi.
Bakteri Endofit Penghasil AHL-Laktonase Asal Tanaman Pisang untuk Pengendalian Penyakit Darah: Endophytic Bacteria Producing AHL-Lactonase from Banana Plant to Control Blood Disease Abidin, Anugrawati; Giyanto; Nawangsih, Abdjad Asih
Jurnal Fitopatologi Indonesia Vol. 20 No. 6 (2024): November 2024 - IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.6.276-285

Abstract

Penyakit darah yang disebabkan oleh Ralstonia solanacearum Phylotype IV merupakan penyakit penting pada pisang yang menyebabkan kehilangan hasil hingga 100%. Bakteri R. solanacearum mengekspresikan gen virulensi melalui mekanisme quorum sensing (QS) dengan menggunakan asil homoserin lakton (AHL) sebagai autoinducer. Enzim AHL laktonase diproduksi oleh bakteri dan memiliki potensi sebagai agens biokontrol bakteri patogen melalui anti-QS. Tujuan dari penelitian ini ialah memperoleh isolat bakteri endofit penghasil AHL laktonase untuk pengendalian R. solanacearum pada tanaman pisang. Tahapan penelitian mencakup isolasi dan seleksi bakteri endofit asal tanaman pisang sebagai penghasil AHL laktonase dengan bioassay maupun molekuler, uji keefektifan isolat bakteri dalam menekan faktor virulensi R. solanacearum, uji keefektifan pengendalian R. solanacearum in planta, dan identifikasi molekuler isolat bakteri potensial. Isolasi bakteri endofit berhasil dilakukan dari sampel pisang asal Bogor di tiga kecamatan, yaitu Cisarua, Dramaga, dan Jonggol yang terdiri atas lima jenis pisang masing-masing menghasilkan 152, 204, dan 130 isolat bakteri endofit. Uji lebih lanjut menggunakan Chromobacterium violaceum sebagai bioindikator menunjukkan lima isolat dengan aktivitas anti-QS. Deteksi gen aiiA dengan PCR menggunakan primer spesifik menunjukkan bahwa kelima isolat memiliki amplikon berukuran 753 pb dan telah dikonfirmasi melalui sekuensing sebagai gen aiiA. Kelima isolat bakteri tersebut CBP1, DBT17, DBT7, DBK2, dan CBN13 mampu menekan pembentukan ekstraseluler polisakarida (EPS) yang dihasilkan R. solanacearum dengan persentase penekanan masing-masing sebesar 75.67%, 90.81%, 87.67%, 92.72%, 81.08%, dan mampu menghambat gejala layu sebagai salah satu gejala penyakit darah pada tanaman pisang. Hasil perunutan gen aiiA menunjukkan bahwa kelima isolat homolog dengan Bacillus cereus, B. thuringiensis, B. substilis, B. amyloliquefaciens.
Co-Authors . Giyanto . Giyanto Abdul Munif ABDUL MUNIF ABDUL MUNIF Abidin, Anugrawati Af'idzatuttama Af’idzatuttama Aisyah Imas Alina Akhdiya Anak Agung Keswari Krisnandika Anja Meryandini Aris Tri Wahyudi Asysyuura, Asysyuura Boedi Tjahjono Budi Marwoto DIMAS ANDRIANTO Edelwinna, Tiara EDI HUSEN Efi Toding Tondok Eka Wijayanti Eka Wijayanti Ely Lailatul Maghfiroh Eny Widajati Erina Sulistiani Firdaus, Muhamad Fajar Fitri Fatma Wardani Giyanto Gustan Pari Gustan Pari Haerani Haerani Hanudin Hanudin Husda Marwan Ida Parida IKA DAMAYANTI Iman Rusmana Imas Aisyah isnainy dinul Mursyalatiyus Juang Gema Kartika Kikin H Mutaqin Krisnandika, A. A. Keswari Marianah, Lisa Meity S. Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meliah, Siti Muhammad Evan Nurrahmawan Purwanto, Ukhradiya Magharaniq Safira RAHAYU FITRIANI WANGSA PUTRIE Sigiro, Ria Heni Siti Zulaiha Sudir Sudir, Sudir Sulistiyani Suryo Wiyono Tita Widjayanti Tita Widjayanti . Toding Tondok, Efi TRI ASMIRA DAMAYANTI Umi Fatmawati Wati, Cheppy Wiraswati, Sri Martina Yana Anisa . YULIN LESTARI Zhenita Vinda Tri Handini