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All Journal International Journal of Public Health Science (IJPHS) Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Forestry Research Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Penelitian Hasil Hutan Indonesian Journal of Tropical and Infectious Disease Jurnal Penelitian Ekosistem Dipterokarpa Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Jurnal Ilmiah Rekayasa Pertanian dan Biosistem Health Notions Buletin Farmatera Jurnal Respirasi (JR) Biomolecular and Health Science Journal Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Edukasi: Journal of Educational Research AKSONA Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal) Folia Medica Indonesiana
Abdurachman Abdurachman
Department Of Anatomy And Histology, Faculty Of Medicine, Universitas Airlangga, Surabaya
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Journal : The Indonesian Biomedical Journal

 1 
Dedifferentiation of MCF-7 Breast Cancer Continuous Cell Line, Development of Breast Cancer Stem Cells (BCSCs) Enriched Culture and Biomarker Analysis Ami Ashariati Prayogo; Andi Yasmin Wijaya; Winona May Hendrata; Steven Sheng Looi; Reny I’tishom; Lukman Hakim; Fedik Abdul Rantam; I Ketut Sudiana; Abdurachman Abdurachman
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.977

Abstract

BACKGROUND: Cancer stem cells (CSCs) eradication might serve as a robust approach for cancer eradication. MCF-7 as breast cancer continuous cell line is known to contain breast CSCs (BCSCs) for its capability to maintain its original tumor population. CSCs enriched culture is a fundamental tool for CSCs targeted therapy development. Effective and unsophisticated CSCs dedifferentiation protocol for producing CSCs enriched culture is needed.METHODS: MCF-7 cells were cultured initially in Dulbecco's Modified Eagle Medium (DMEM) low glucose medium then changed to DMEM:F12. Serum starvation was performed during each medium refreshment gradually with fetal bovine serum (FBS) concentration of 10%, 5%, 2.5% until reaching 1% FBS concentration. Stable MCF-7 culture was then adapted to serum free culture system, containing DMEM:F12, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and B27 supplement as dedifferentiation protocol for 18 days. Cluster of differentiation (CD)44 and CD24 double staining immunocytochemistry was performed to evaluate cell stemness.RESULTS: The population of cells expressing BCSCs markers (CD44+/CD24low) in non-adherent single cells subpopulation was significantly increased after the dedifferentiation procedure (70.39%) compared to control groups (0.71%) (p<0.05). In contrast, the expression of BCSCs marker in adherent single cells subpopulation and for both adherent and non-adherent mammosphere the BCSCs markers showed a stable expression.CONCLUSION: BCSCs enrichment of breast cancer cell cultures from MCF-7 breast cancer cell line can be performed. Breast cancer cell plasticity is observed during the dedifferentiation protocol. Development of dedifferentiation inducing protocols can serve as an important foundation for breast cancer therapy development through BCSCs elimination.KEYWORDS: breast neoplasms, cell line, dedifferentiation, immunohistochemistry, neoplastic stem cells
Co-Authors Agus Ismanto Agus Rubiyanto Ahmad Sukandar Ami Ashariati Amiril Saridan, Amiril Andi Yasmin Wijaya Andianto Andianto, Andianto Andika Darmawan Wahono Arinda Putri Auna Vanadia Asmaul Lutfi Marufah Astri Dewayani Atika Atika Atika Atika Atsira, Olga Putri Bambang E. Suwito Bambang Edi Suwito Bambang Purwanto Bambang Purwanto Barly Barly Bonfilio Neltio Ariobimo Chorisma Permata Putri Nurazizah Dian Eka Indriani, Dian Eka Dion K. Dharmawan Dominicus Martono, Dominicus Dzanuar Rahmawan Efrida Basri Erikavitri Yuliati Erliza Noor Farida Herry Susanty Fedik Abdul Rantam Hanafiah Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hilmy Yafi Zuhair I'tishom, Reny Ida Lanniari, Ida Irwadi, Irfiansyah Jamal Balfas Jasni Jasni Khairina Khairina Krisdianto Sugiyanto Krisnawan A. Pradana Krisnawan Andy Pradana Lukman Hakim Luqman Hakim Andira Margarita Maramis, Margarita Marjono Dwi Wibowo Martin, Tomas Marcelino A. Martono Tri Utomo Massofian Noor, Massofian Musa Musa Nurwati Hadjib Oktariza, Rury Tiara Olga Putri Atsira Prastiya Indra Gunawan Pratiwi, Wahyu Nur Rahmawan, Dzanuar S.Pd. M Kes I Ketut Sudiana . Saefudin Saefudin Sita Setyowatie SOETJIPTO . Steven Sheng Looi Subagyo Subagyo Sunarti, Titi Chandra Tajuddin Bantacut Taya Elsa Savista Tjahjono, Agus Eko Ujianto Ujianto Ulfi Qomariyah Hanum Viskasari P. Kalanjati Wahyu N. Pratiwi Winona May Hendrata Yudi Her Oktaviono, Yudi Her Yuliati, Erikavitri