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All Journal Indonesian Journal of Pharmaceutical Science and Technology Universa Medicina The Avicenna Medical Journal Journal of Science and Technology Research for Pharmacy Indonesian Journal of Cancer International Journal of Cell and Biomedical Science Indonesian Journal of Biomedicine and Clinical Sciences Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Medical and Pharmaceutical Science
Ibrahim, Sugeng
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Time-Dependent Secretion of IFN-γ, TNF-α, Perforin, and Granzyme-B in CTL-Conditioned Medium from Colorectal Cancer Patient Ibrahim, Sugeng; Putra, Agung; Hidayah, Nurul; Khan, Ahmed Faheem; Nugraha, Dendi Krisna
The Avicenna Medical Journal Vol. 6 No. 1 (2025): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v6i1.46889

Abstract

Background: Cytotoxic T lymphocytes (CTLs) are key effectors of adaptive immunity, exerting their functions through the release of pro-inflammatory cytokines and cytotoxic molecules. Conditioned medium (CM) derived from CTLs has emerged as a potential cell-free immunotherapeutic strategy; however, the temporal dynamics of its secreted mediators remain poorly defined. Methods: Human CTLs were isolated, activated, and cultured in vitro. CM was harvested at defined intervals (days 5, 10, and 15). Concentrations of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), perforin, and granzyme-B were quantified by enzyme-linked immunosorbent assay (ELISA). Temporal secretion profiles were evaluated using ANOVA with post-hoc analysis. Results: IFN-γ and TNF-α exhibited peak secretion at day 5, followed by a decline at later time points. In contrast, perforin and granzyme-B increased progressively, reaching maximal levels at day 15. All four mediators demonstrated significant time-dependent variation (p < 0.05). Conclusion: CTL-derived CM displays distinct time-dependent secretion patterns, with cytokines predominating during early activation and cytotoxic molecules dominating later phases. These findings underscore the importance of optimizing CM collection timing to maximize its immunomodulatory and therapeutic potential, and provide a rationale for further translational development of CTL-CM in immunotherapy.
Exosome Hypoxic-MSCs, Glutathione, and Vitamin C: Effect on IL-10 Levels and CD-163 Expression Utami, Wulan Dyah; Muhar, Adi Muradi; Sumarawati, Titiek; Putra, Agung; Setiawan, Eko; Ibrahim, Sugeng; Taskworo, Dodik; Haitamy, Mohammad Nurrizki
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 3 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i3.60941

Abstract

Hyperpigmentation of the skin is a result of ultraviolet B (UVB) exposure, which causes oxidative stress due to increased reactive oxygen species (ROS), leading to various skin problems, including melanin accumulation. Exosomes can affect melanocyte activity. Exosomes, as small vesicles released by cells, can affect melanocyte activity and play an important role in various hyperpigmentation processes. The study aims to determine the effect of exosome mesenchymal stem cell hypoxia (EH-MSC) and glutathione with vitamin C on IL-10 levels and CD163 expression. IL-10 gene expression was measured using qRT-PCR, while CD163 expression was analyzed via immunohistochemical staining. A total of 30 male C57BL/6 mice were used and randomly assigned to five different treatment groups. The highest expression of IL-10 was observed in the EH-MSCs-treated group (K4), although the difference was not statistically significant compared to the control (p = 0.135). In contrast, the group receiving a combination of EH-MSCs with glutathione and vitamin C (K5) exhibited the highest percentage of CD163 expression, with a statistically significant difference (p = 0.00). These findings demonstrate that the administration of EH-MSC and glutathione with vitamin C significantly increased the expression of CD163, but insignificantly increased IL-10 in C57BL/6 mice with a UVB-induced hyperpigmentation model.
Modulation of inflammatory pathways in ischemic stroke rats by hypoxia-primed umbilical cord mesenchymal stem cells (UC-MSCs): Implications for IFN-γ and IL-10 signaling Fahreza, Rakha; Setiawan, Eko; Trisnadi, Setyo; Putra, Agung; Hidayah, Nurul; Fikriya Novita Sari; Ibrahim, Sugeng
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 4 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i4.24529

Abstract

Ischemic stroke is a major global cause of disability and mortality. Inflammation plays a central role in its pathogenesis, characterized by elevated pro-inflammatory cytokines such as IFN-γ and reduced anti-inflammatory cytokines like IL-10. Mesenchymal stem cells (MSCs), especially those derived from the umbilical cord (UC-MSCs), exhibit enhanced immunomodulatory potential when preconditioned under hypoxia. This study aims to evaluate the effect of hypoxic-preconditioned UC-MSCs administration on IFN-γ and IL-10 levels in an ischemic stroke rat model. This in vivo experimental study employed a randomized posttest-only control group design with four groups of male Wistar rats (n=6 each), ranging from healthy controls, untreated group to stroke-induced groups treated with hypoxic UC-MSCs at different doses (1.5x106 and 3x106). IFN-γ and IL-10 levels in brain tissue of each group were measured via ELISA. Significant reduction in IFN-γ and elevation in IL-10 were observed in UC-MSC-treated groups, particularly at the 3×10⁶ cell dose compared to the untreated ischemic group (p<0.05). Hypoxic UC-MSCs reduce post-stroke inflammation by lowering IFN-γ and enhancing IL-10, indicating a promising immunomodulatory potential.
Hypoxic MSCs Reduce UV-B Collagen Loss by Modulating CD68 and TNF-α Expression Ati, Sri Umi; Putra, Agung; Sumarawati, Titiek; Setiawan, Eko; Ibrahim, Sugeng; Taskworo, Dodik
Indonesian Journal of Pharmaceutical Science and Technology Vol 13, No 1 (2026)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v13i1.63650

Abstract

Ultraviolet-B (UV-B) radiation-induced skin photoaging is marked by collagen degradation and chronic inflammation, with limited effective treatments currently available. Hypoxic-preconditioned mesenchymal stem cells (H-MSCs) offer a novel therapeutic approach due to their immunomodulatory capabilities. This study evaluated the effects of H-MSCs at two doses (2.5×105 and 5×105 cells) on UVB-induced collagen loss, focusing on CD68 expression and TNF-α levels in a rat model. Male Wistar rats were divided into five groups: healthy controls, UV-B-exposed negative controls (saline), positive controls (hyaluronic acid), and two H-MSC-treated groups. After UV-B exposure (160 mJ/cm², five times per week for two weeks), validated H-MSCs were administered subcutaneously. Collagen content was assessed histologically, while CD68 gene expression and TNF-α levels were measured by qRT-PCR and ELISA, respectively. UVB exposure led to significant reductions in collagen and increased levels of inflammatory markers. H-MSC treatment showed dose-dependent anti-inflammatory effects, with the higher dose (5×105 cells) optimally reducing CD68 expression and TNF-α levels, nearly matching healthy controls. These results suggest that H-MSCs, particularly at higher doses, may be a promising therapy for UVB-induced skin damage and collagen loss.
Co-Authors Agung Putra Amalina, Nur Dina Arda, Adzani Gaisani Ati, Sri Umi Cahyani, Dini Catharina Suharti Chodidjah Chodidjah Chodijah , Chodijah Dewi, Alisia Martha Eko Setiawan Eko Setiawan Fahreza, Rakha Fikriya Novita Sari Fristiani, Yeni Ginanto, Dede Hadi Sarosa Haitamy, Mohammad Nurrizki Ignatius Riwanto, Ignatius Khan, Ahmed Faheem Minidian Fasitasari Mohammad Ariq Nazar Muhar, Adi Muradi Nugraha, Dendi Krisna Nurul Hidayah NURUL HIDAYAH Pramukarso, Dodik Tugasworo Pramukarso Purwaningsih, Hesti Rifai, Fauziah Novita Putri Sadikin, Nadya Audina N Selamat Budijitno Setyo Trisnadi Sri Priyantini Sri Priyantini Mulyani Sulistyo, Sona Taskworo, Dodik Titiek Sumarawati Trisnasi, Setyo Utami, Wulan Dyah