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All Journal HAYATI Journal of Biosciences Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Best Journal (Biology Education, Sains and Technology) Jurnal Zona Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati
Anthoni Agustien
Universitas Andalas
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Biologi Tropis

 1 
Antagonist Test of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the Growth of Magnaphorte oryzae on Several Varieties of Priming Rice Seeds Feskaharny Alamsjah; Zozy Aneloi Noli; Hesti Dwi Marcellinna; Anthoni Agustien; Suwirmen; Kurniadi Ilham
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6112

Abstract

Priming is a seed-soaking technique to increase seed viability and also suppress pathogens' growth. Magnaporthe oryzae is one of the pathogens in rice plants that causes leaf blast disease. The high rice consumption each year in Indonesia is not proportional to the amount of rice plant production, which is affected by the growth of pathogenic fungi. This study aims to determine the viability and percentage inhibition of Bacillus subtilis and Trichoderma harzianum against the growth of the pathogenic fungus Magnaporthe oryzae on local West Sumatra rice varieties Ceredek, Pandan Pulau, and Batang Sungkai. The research was conducted by testing the viability of microbes by counting the number of colonies and testing microbial antagonists with the Dual Culture method on seven days of observation. The results showed that Bacillus subtilis and Trichoderma harzianum could inhibit the growth of the pathogen M. oryzae, which causes leaf blast disease. The viability of Bacillus subtilis after biopriming for 48 hours was most significant on Ceredek variety, which was 15.9x106 cfu/g, and for Trichoderma harzianum on Pandan Pulau variety, which was 0.4x106 cfu/g. The most significant inhibition percentage of Bacillus subtilis and Trichoderma harzianum was obtained in Ceredek rice with a value of 30.13% (medium category) and 63.04% (high category).
Growth Curve and Antibacterial Activity Test of Endophytic Bacteria Isolates 1 (IBE1) from Labu Koteka (Lagenaria siceraria) Against Escherochoa coli, Pseudomonas aeruginosa and MRSA Putri, Silvy Rizka; Djamaan, Akmal; Agustien, Anthoni
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8506

Abstract

The rising incidence of antibiotic-resistant bacteria presents a considerable global health threat, requiring the immediate creation of new antimicrobial drugs. A possible strategy involves investigating antibiotics sourced from endophytic bacteria, which are microorganisms that inhabit plant tissues symbiotically. This research is to assess the inhibitory efficacy of endophytic bacteria derived from Lagenaria sicerari against, Escherichia coli, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus (MRSA). The identified endophytic bacteria were cultivated in a fermentation medium to synthesize antibiotics, and their growth curves were examined. Subsequently, antibacterial activity assays were performed against the three harmful bacteria, measuring the sizes of the clear inhibitory zones. The results indicated that Isolated Bacteria Endophytic (IBE) 1 attained optimal inoculum levels at 24 hours and sustained the stationary phase from 24 to 36 hours. The isolates exhibited optimum fermentation on a medium utilizing glucose as the carbon source, with a carbon-to-nitrogen ratio of 5. IBE 1 had the most effective antibacterial action against P. aeruginosa, with a moderate inhibition characterized by clear zone widths of 10.80 mm. The findings indicate that endophytic bacteria from Lagenaria siceraria may serve as promising sources of new antimicrobial drugs, especially against Pseudomonas aeruginosa.
Co-Authors - Jumawita . Mansyurdin Aghni Aznia Akmal Djamaan Alviche, Lola Anggun Sophia Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dewi Yudiana Shinta Dianty Putri Dina Wahyuna Djong Hon Tjong Dwitaviani, Rima Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Herwita Idris Hesti Dwi Marcellinna Ikel Fitri Yanis Ilham, Kurniadi Ilsa Septia Putri Irdawati Irdawati Irfan Mustafa Jetty Nurhajati Kurniadi Ilham Kustiasih Lestari Linar Z. Udin Lola Alviche Marlina Marlina Melisa Ainil Fajri Mira Rosnawita Muhammad Diki Juliandi Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Periadnadi Periadnadi Pingkan Aditiawati PINGKAN ADITIAWATI Putri, Silvy Rizka Rahmadani Eka Pitri Rahmayati, Riesca Salsabilah Rahmi Adila Rahmi, Inayati Riesca Salsabilah Rahmayati Riski Budi Yani RR. Ella Evrita Hestiandari Rusnam Rusnam Suwirmen, Suwirmen Syafrita, Assyifa Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Triyatdipa, Haqil Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Wiwik Handayani Yetria Rilda Yetti Marlida Yurike Nova Edlin Zakaria, Indra Junadi Zovia, Miftahul Zozy Aneloi Noli Zuhri Syam Zuhri Syam