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Journal : Medical Laboratory Technology Journal

Condition Optimization for The Analysis of Risperidone and 9-0H-Risperidone by High-Performance Liquid Chromatography Luki Yogaswara Yusuf; Aliya Nur Hasanah; Melisa Intan Barliana
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.614 KB) | DOI: 10.31964/mltj.v0i0.424

Abstract

Risperidone (RIS) is one of the most widely used atypical antipsychotics for treating schizophrenia in hospitals. RIS is metabolized by the liver and produces the primary active metabolite  9-OH-Risperidone (9-OHR). In the process of RIS metabolism, it is suspected that there are gene polymorphisms that cause variations in patient responses. Analysis of RIS and 9-OHR levels in the patient's blood can help to explain the various responses. High-Performance Liquid Chromatography (HPLC) is the most popular method to analyze RIS and 9-OHR, but many deficiencies were found in the chromatograms in the previous study. This research aims to obtain optimal conditions of the analysis prior to method validation. Condition optimization by optimizing the wavelength, composition of the mobile phase, pH, flow rate, and particle size of the stationary phase. The results showed that the wavelength was 279 nm, the mobile phase was 0.05 M KH2PO4 pH 3.7: acetonitrile (94:6, v/v) plus 0.3% triethylamine, and the flow rate was 1.2 ml/min in the stationary phase (LiChroCART® RP 18; 250x4 mm; 10 µm) being the optimal condition. This method is suggested to continue method validation for analyzing RIS and 9-OHR in the serum or plasma.
Co-Authors Ade Zuhrotun Ahmad Muhtadi AKBAR ROZAAQ MUGNI Akhmad Sobarudin Amelia Soyata Andini, Alfita Nur Anis Khoerunisa Anis Khoerunisa, Anis Anisa Desy Ariyanti Antonius Herry Cahyana Ardiansah, Bayu Badriah Afriani Buchari - Chandra, Felix Christina Damayanti Danni Ramdhani Driyanti Rahayu Ellin Febrina Emil Budianto Emil Budianto Fenadya Rahayu Agustriono Fikri Nazaruddin Firdha Senja Maelaningsih GHALIB SYUKRILLAH SYAHPUTRA Gia A. Faradiba Hendra Wijaya Henny Purwaningsih Henry Ng Henry Ibrahim Dalli Ida Ayumiati Ida Musfiroh Ida Musfiroh Ida Musfiroh Ida Musfiroh, Ida Ike Susanti Ike Susanti Jayadi, Inneke Karen Low Ka Ling Kelvin F. Pratama Laffyanto, Dionisius Luki Yogaswara Yusuf Luluk Luqyana Zahrah Taqiudina Mahdiyah Maryam Nur Afifah Maryana, Rianti Mawaddah, Nadiya Widda Meilia Suherman Meilia Suherman, Meilia Melisa Intan Barliana Merly, Siti Michelle Ferdinand Muchtaridi Muchtaridi Muhammad Fauzi Muhammad Rizki Nugraha Mulyadi, Ananda Putri Aulia Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Nadia Fauziah Rahmadiani Nasrul Wathoni Noer Erin Meilina Nur hayati Nurike Susendi Nyi Mekar Saptarini Priska Primandini Priskila O Dwiestri Priskila O Dwiestri, Priskila O Putri Nur Azizah, Putri Nur Rahmah, Raisya Rahmana Emran Kartasasmita Rembulan Kusmawati Rembulan Kusmawati, Rembulan Rieda Nurwulan Septyani Rimadani Pratiwi Rr. Sulistiyaningsih Shelvy Elizabeth Suherman Slamet Ibrahim Soni, Dang Sri Adi Sumiwi Sri Agung Fitri Kusuma Sri Agung Fitri Kusuma, Sri Agung Sriwidodo B Sriwidodo B, Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo, Sriwidodo Sudirman Sudirman Sukri, Tony Taofik Rusdiana Utama, Aris Eka Utari, Triesye VANIA PUTRI Widajanti Wibowo Wisnu A. A Wiwiek Indriyati Wiwiek Indriyati, Wiwiek Yoga Windhu Wardhana Yoppi Iskandar