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Journal : SIMBIOSIS

UJI AKTIFITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BUAH MANGGIS (Gracinia mangostana L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Putri Permata; Retno Kawuri; AA Ketut Darmadi
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (987.139 KB) | DOI: 10.24843/JSIMBIOSIS.2018.v06.i01.p02

Abstract

Mangosteen rind has a benefical compounds liked xanthone which consisting of mangostin, mangosterol, mangostinon A and B, trapezifolixanthone, tovophyllin B, alpha and beta mangostin, garcinon B, mangostanol, gartanin, and flavonoid epikatekin that known contain an antibacterial and antidiare agent. Escherichia coliis an opportunistic bacterium that is commonly found in the human colon as a normal flora. Its unique because it can cause primary infection in the intestine such as diarrhea in children, as well as its ability to cause infection in other body tissues outside the intestine.This research was done from December 2016 to March 2017. The methods that used in this research were well diffusion test to know the inhibitory power by measuring the clear zone formed. measure the clear zone of mangosteen rind against Escherichia coli. Then to testing extract compounds, there was used phytochemicals screening test. Data there has been found will be analyzed through analysis of variance (ANOVA). In the inhibitory test of ethanol extract from mangosteen rind (G. mangostana L.), it was so effective to inhibit the growth of Escherichia coli through in-vitro test with a resistivity of 1,58 cm and categorized as a strong inhibitory, which MIC from mangosteen rind against Escherichia coli was 3,9% and the compounds that contain in ethanol extract from mangosteen rind (G. mangostana L.) were alkaloid, phenolik, flavonoid, saponin and terpenoid. Key word : antibacterial activity, Garcinia mangostana L., Escherichia coli.
INVENTARISASI GULMA PADA TANAMAN JAGUNG (Zea mays L.) DI LAHAN SAWAH KELURAHAN PADANG GALAK, DENPASAR TIMUR, KODYA DENPASAR, PROVINSI BALI SURYANINGSIH -; MARTIN JONI; A.A KETUT DARMADI
SIMBIOSIS Vol I, No 1, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.033 KB)

Abstract

This study aims to determine the weed species that exist on Zea mays L. plant in paddyfields Padang Galak village, East Denpasar, Bali Province. This study is useful to provideinformation about the species of weeds found in maize (Zea mays L.) in the fields of biology andagriculture, so weed control on Zea mays L. Plant can be implemented.Research was conducted from January 7 until March 11, 2011. In this study used themethod of cruising (exsploration sampling). To be more efficient and systematic results, alsoused a systematic method of lines.The results found 36 weed species belonging to 20 families. The distribution of theweed species, was found in Zea mays L. plant age of 4 weeks, which composed of 8 families 16Species. Weeds found in Zea mays L. plants aged of 6 weeks (the formation of fruit) consistedof 10 familes and 23 species. While weeds found in Zea mays L. plants 8 weeks of age(ripening fruit) were 14 families and 28 species. Weeds found in Zea mays L. plants aged 10weeks (before harvest) comprised of 20 families and 36 species.
EFEKTIVITAS EKSTRAK BUNGA KENANGA (Cananga odorata) UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Colletotrichum acutatum PENYEBAB PENYAKIT ANTRAKNOSA PADA TANAMAN CABAI BESAR (Capsicum annum) Silviana Febrionita Meiskia Agung; Sang Ketut Sudirga; Anak Agung Ketut Darmadi
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.673 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i01.p10

Abstract

Jenis Colletotrichum acutatum merupakan jamur patogen penyebab penyakit antraknosa pada tanaman budidaya, salah satunya tanaman cabai. Pengendalian penyakit antraknosa umumnya menggunakan pestisida sintetis. Penggunaan pestisida sintetis secara terus menerus dapat menimbulkan efek samping yang merugikan, sehingga diperlukan senyawa lain untuk mengendalikan jamur patogen tersebut salah satunya melalui penggunaan fungisida nabati. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak bunga kenanga dalam menghambat pertumbuhan Colletotrichum acutatum dan untuk mengetahui golongan senyawa aktif yang terkandung dalam ekstrak bunga kenanga. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan yaitu P0: kontrol negatif, P1: kontrol positif, P2: konsentrasi ekstrak 1% (b/v), P3: konsentrasi ekstrak 2% (b/v), P4: konsentrasi ekstrak 3% (b/v), P5: konsentrasi ekstrak 4% (b/v), P6: konsentrasi ekstrak 5% (b/v) dengan masing-masing perlakuan diulang sebanyak 4 kali. Parameter yang diamati yaitu terbentuknya diameter zona hambat, diameter koloni, kerapatan spora, dan uji fitokimia ekstrak. Hasil penelitian uji sumur difusi menunjukan zona hambat terbesar terdapat pada konsentrasi 5% (P6) yaitu sebesar 10,05 mm dan zona hambat terkecil pada konsentrasi 1% (P2) sebesar 7,53 mm. Hasil uji koloni menunjukan diameter koloni terkecil pada hari ke-10 terdapat pada konsentrasi 3% (P4) sebesar 7,09 cm dan diameter koloni terbesar pada konsentrasi 1% (P2) sebesar 8,46 cm. Hasil uji kerapatan spora menunjukan jumlah kerapatan spora terbesar terdapat pada konsentrasi 1% (P3) yaitu 3,24×104 spora/mL dan jumlah spora terkecil terdapat pada konsentrasi 3% (P4) sebesar 2,36×104 spora/mL. Golongan senyawa aktif yang ditemukan dalam ekstrak bunga kenanga meliputi alkaloid, steroid, flavonoid, fenolik, saponin dan tanin.
GROWTH OF SEVERAL SPECIES OF MANGROVE SEEDLINGS AS SEEDS READY FOR PLANTING IN, KARHUTLA CENTRE OF JAVA, BALI, AND NUSA TENGGARA I Ketut Endra Primantara; A.A.Ketut Darmadi; I Ketut Ginantra
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (769.359 KB) | DOI: 10.24843/JSIMBIOSIS.2019.v07.i01.p02

Abstract

Mangrove forests have important role in coastal ecosystems, for example to overcome marine abrasion, bird nesting sites, forming ecological balance, capturing and localizing sediments, preventing soil acidity and inhibiting sea water intrusion. The success of mangrove reforestation is largely determined by the success in preparing mangrove seedlings. Nursery beds made of bamboo to accommodate 4 species of mangrove seedlings, that are Bruguiera gymnorrhiza, Rhizopora apiculata, Rhizopora mucronata and Rhizopora stylosa with 6 replications and each of 3 individuals of each type. The variables measured were plant height, number of leaves and stem diameter. Statistical analysis with the Costat & Co. program The results showed that the average growth of stem height from the 4 mangrove seedlings measured from 6 -13 MST was 1.5 cm, 1.4 cm, 0.8 cm and 0.7 cm respectively. The average number of leaves from the 4 mangrove seedlings were 0.6, 1.2, 0.6, and 0.6 strands respectively and the average stem diameter of the 4 mangrove seedlings was 0.1 cm. The mangrove seedlings of Rhizopora mucronata and Bruguiera gymnorrhiza species that produced higher growth rates of stem height than others, and Bruguiera gymnorrhiza which produced the highest average number of leaves compared to the other three types. Keywords ; seedlings, mangroves, growth
UJI VIABILITAS SERBUK SARI SECARA In-Vitro KELAPA (Cocos nucifera L.“Rangda”) DENGAN WAKTU DAN SUHU PENYIMPANAN YANG BERBEDA Sista Nirmala; Eniek Kriswiyanti; AA. Ketut Darmadi
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.552 KB)

Abstract

This aims of the research was to determine the structure and pollen viability of RangdaCoconut (Cocos nucifera L. “Rangda”) stored at different time and temperatures. Pollen sampleswere collected from Gianyar, Klungkung and Negara. The experiment was conducted at theLaboratorium of Forensic and the Laboratorium of Plant Development and Structure, UdayanaUniversity, from October 2012 to January 2013. Pollen structure was analyzed with acetolysemethod, while pollen viability was analyzed in-vitro. The results showed that the structure of thepollen of “Rangda” coconut was circular, media, monosulcate, Subferoidal-Prolat, Sferoidal.Pollen viability of “Rangda" coconut was low (less than 3%). Pollen stored in the freezer has thebest viability, which continually increased until the 2nd weeks, then declined after that, whiepollen viability stored at room temperature tend to decline up to the fourth weeks.Keywords: structure, viability, pollen, time, storage
KAJIAN ETNOBOTANI OBAT TRADISIONAL MASYARAKAT SUKU BATAK DI DESA LAWE PERBUNGA, KECAMATAN BABUL MAKMUR, ACEH TENGGARA Alprida Yanti; Eniek Kriswiyanti; A. A. Ketut Darmadi
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.419 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p02

Abstract

Traditional medicine is a mixture of natural ingredients that have been used for generations for treatment based on experience which is inherited from their ancestors. Traditional medicine systems are generally closely related to religious aspects and also use natural elements such as plants, animals, and minerals. One of the people who still use traditional medicine is the Batak tribe in Lawe Perbunga, Babul Makmur Sub-district, Southeast Aceh. This study aims to examine the types of plants, plant parts used, processing methods and the use of traditional medicines is the Batak tribe in Lawe Perbunga, Babul Makmur Sub-district, Southeast Aceh. The method used in this research is purposive sampling and snowball to determine 40 respondents. Data collection techniques used interviews, observation, documentation and literature study. Data analysis used qualitative and quantitative descriptive analysis techniques. The types of plants used as ingredients for traditional medicinal consist of 21 species and 18 family for the treatment of 20 types of diseases. The plant parts used as medicinal ingredients, respectively, were leaves, fruit, tubers, rhizomes, sap, stems and roots respectively 48,39%, 12,90%, 12,90%, 9,68%, 6,45%, 6,45%, and 3,23%. Processing of traditional medicinal ingredients in 5 ways, namely without being processed, boiled, sliced, brewed and roasted, respectively, by 43,75%, 20,31%, 20,31%, 9,38% and 6,25%.The use of traditional medicine are carried out in 4 ways, namely drinking, eating, smearing, and affixing respectively 45,59%, 29,41%, 17,65% and 7,35%. Keywords: Lawe Perbunga Village, use, processing, medicinal plants