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All Journal Medical Laboratory Technology Journal AcTion: Aceh Nutrition Journal Jaringan Laboratorium Medis An-Nadaa: Jurnal Kesehatan Masyarakat Tropical Health and Medical Research Journal Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Karya Generasi Sehat Jurnal Praba: Jurnal Rumpun Kesehatan Umum
Aima Insana
Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin, Indonesia
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Salıva pH, Carıes and OHI-S Score in Stuntıng Pre-School Chıldren in The Swamp Area Utami, Naning Kisworo; Andrestian, Meilla Dwi; Insana, Aima
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.688

Abstract

Stunting remains a major global health issue and is associated with impaired physical and cognitive development, as well as increased susceptibility to oral diseases such as dental caries. Environmental factors, including acidic peatland water, may further aggravate oral health conditions among children living in swamp areas. This study aimed to analyze the relationship between salivary pH, dental caries severity (def-t index), and oral hygiene status (OHI-S) in preschool children with stunting living in peatland regions of South Kalimantan, Indonesia. An analytical observational study with a cross-sectional design was conducted involving 32 preschool children aged 2–5 years residing in the working area of the Martapura Timur Public Health Center. Data collection included clinical examination of caries using the WHO criteria, salivary pH measurement with a Krisbow digital pH meter, and assessment of oral hygiene using the Simplified Oral Hygiene Index (OHI-S). Statistical analysis was performed using the Spearman correlation test. The results showed that salivary pH ranged from 5.5 to 7.0, def-t scores ranged from 2 to 8 (mean = 5.34 ± 1.78), and OHI-S scores were 0.00 in 22 children (68.7%) and >0.00 in 10 children (31.3%). A significant association was found between salivary pH and dental caries severity (p = 0.005; α = 0.05), indicating that lower salivary pH is associated with higher caries prevalence. In conclusion, stunted preschool children in peatland areas exhibited acidic salivary conditions and a high caries burden despite relatively good oral hygiene status. These findings highlight the importance of integrating nutritional rehabilitation and oral health education programs to reduce the risk of dental caries in children living in environmentally vulnerable swamp regions.
Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Abstract

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.
PERBEDAAN KUALITAS PEWARNAAN SEDIAAN SITOLOGI MENGGUNAKAN BUNGA TELANG (Clitoria ternatea L) SEBAGAI ALTERNATIF PENGGANTI METHYLENE BLUE PADA METODE DIFF QUICK Rizky Arsyanti; Neni Oktiyani; Aima Insana; Ratih Dewi Dwiyanti
Jurnal Karya Generasi Sehat Vol. 3 No. 2 (2025): Edisi Desember 2025
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v3i2.232

Abstract

Pewarnaan sediaan sitologi dilakukan untuk memperjelas morfologi sel agar dapat diamati secara mikroskopis, khususnya pada bagian inti sel dan sitoplasma. Pewarnaan Diff Quick merupakan metode yang umum digunakan karena cepat dan memiliki kontras warna yang baik, tetapi salah satu komponennya, methylene blue, bersifat toksik dan tidak ramah lingkungan. Penelitian ini bertujuan untuk mengetahui perbedaan kualitas pewarnaan sediaan sitologi menggunakan larutan bunga telang (Clitoria ternatea L) sebagai alternatif alami pengganti methylene blue dengan pewarnaan Diff Quick. Jenis penelitian True Experiment dengan rancangan Post Test Only with Control Group Design, terdiri dari 4 perlakuan dengan 6 kali pengulangan menggunakan 1 sampel cairan pleura yang diwarnai dengan larutan bunga telang pada konsentrasi 20%, 25%, dan 30%, serta dibandingkan dengan pewarnaan Diff Quick standar sebagai kontrol. Hasil pemeriksaan mikroskopik larutan bunga telang konsentrasi 20% menunjukkan kualitas tidak baik, 25% menunjukkan kualitas cukup baik dan 30% menunjukkan kualitas pewarnaan yang paling mendekati pewarnaan standar dengan kategori “sangat baik.” Uji Friedman menunjukkan perbedaan yang signifikan antar kelompok perlakuan (p=0,001), dan uji Wilcoxon menunjukkan tidak terdapat perbedaan signifikan antara larutan bunga telang 30% dengan kontrol (p=0,063). Kesimpulannya, larutan bunga telang konsentrasi 30% memiliki potensi sebagai pewarna alami alternatif methylene blue dalam Metode Diff Quick. Disarankan untuk dilakukan penelitian lebih lanjut dengan menaikkan konsentrasi larutan pewarna bunga telang atau menaikkan pH larutan pewarna bunga telang (Clitoria ternatea L).
Development and evaluation of functional “Harukam” jelly gum for oral health in stunted children Andrestian, Meilla Dwi; Utami, Naning Kisworo; Insana, Aima; Noraini, Noraini
AcTion: Aceh Nutrition Journal Vol 10, No 4 (2025): December
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v10i4.2991

Abstract

Snakehead fish (Channa striata) bones exhibited antibacterial activity against cariogenic bacteria, while Banjar Siam orange (Citrus reticulata) provided complementary antibacterial effects. The combination of these ingredients has the potential to be formulated into a functional saliva-stimulating jelly gum to support oral health in stunted children. This study aimed to obtain an optimal formulation of “Harukam” jelly gum as a saliva stimulant for caries prevention. The research was conducted at the Health Polytechnic of the Ministry of Health Banjarmasin and the Industrial Service Standardization Center Banjarbaru, Indonesia, from June to October 2025. A completely randomized design was applied using four concentrations of snakehead fish bone flour: P0 (0%), P1 (1%), P2 (2%), and P3 (3%). Sensory evaluation was performed using the Hedonic Scale, while the chemical composition was determined through proximate analysis. Optimization was determined using the DeGarmo method, and statistical analysis was performed using the Friedman test. The addition of snakehead fish bone flour significantly affected sensory acceptance (p < 0.001) and increased most proximate values, except for carbohydrates. The P1 formulation received the highest preference score. In conclusion, P1 “Harukam” jelly gum showed potential as a functional food to stimulate saliva secretion and support caries prevention in stunted children.
The Value of the TB IgG/IgM Rapid Test and Erythrocyte Sedimentation Rate in Relation to Xpert MTB/RIF Tests in the Diagnosis of Pulmonary Tuberculosis Sari, Endah Permata; Lutpiatina, Leka; Insana, Aima
Tropical Health and Medical Research Vol. 8 No. 1 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tuberculosis (TB) remains a global health problem, requiring healthcare workers to have accurate and effective diagnostic methods. The Xpert MTB/RIF-based Molecular Rapid Test is recommended as the standard for TB diagnosis, but in clinical practice, healthcare workers still frequently use the TB IgG/IgM Rapid Test and the Erythrocyte Sedimentation Rate (ESR) as complementary tests. This study aimed to determine the relationship between Xpert MTB/RIF test results and the TB IgG/IgM Rapid Test and ESR in pulmonary tuberculosis patients at Pertamina Hospital, Balikpapan. This study used a retrospective, observational analytical study design utilizing secondary data from laboratory tests from January–December 2024. Researchers analyzed data from 250 patients who met the inclusion criteria. They analyzed the relationship between variables using the Spearman correlation test. The results showed that 16.4% of patients had MTB detected on the Xpert MTB/RIF test. Statistical analysis showed a significant positive correlation between Xpert MTB/RIF results and the TB IgG/IgM Rapid Test, with a moderate correlation strength (r = 0.562; p < 0.001). Furthermore, the analysis also showed a significant correlation between Xpert MTB/RIF results and ESR (r = 0.183; p = 0.004), but with a very weak correlation strength. The majority of patients with MTB detected by Xpert MTB/RIF had positive TB IgG/IgM Rapid Test results and abnormal ESR values. This study shows that the TB IgG/IgM Rapid Test and ESR are associated with Xpert MTB/RIF results. However, both tests have limited specificity and can therefore only be used as supporting tests and cannot replace molecular testing in the diagnosis of pulmonary tuberculosis.
The Role of Biological and Environmental Factors in Infant Pneumonia: Findings from the 2023 Indonesian Health Survey Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima
Tropical Health and Medical Research Vol. 8 No. 1 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v8i1.150

Abstract

Pneumonia continues to be a leading cause of morbidity and mortality in infants, particularly in developing countries, making risk factor analysis crucial to support evidence-based prevention efforts. This study analyzed factors associated with pneumonia in 28,610 infants aged one year and under using data from the 2023 Indonesian Health Survey to provide a national overview of the distribution of cases and their determinants. This study used a cross-sectional design and analyzed demographic variables, health characteristics, and environmental factors using descriptive analysis, chi-square tests, and odds ratios. The study results showed a pneumonia prevalence of 0.9%, with an increased risk in low birth weight infants (OR 2.425; 95% CI: 1.688–3.483) and infants with a history of diarrhea (OR 2.051; 95% CI: 1.211–3.475), while breastfeeding reduced the risk of pneumonia (OR 0.590; 95% CI: 0.447–0.778). The data also showed greater susceptibility among male infants and those living in urban areas. These findings confirm that pneumonia in infants is influenced by a combination of biological, environmental, and behavioral factors, so public health interventions need to strengthen monitoring of at-risk infants, increase the coverage and quality of breastfeeding, prevent diarrhea, and control exposure to air pollution, especially in urban areas. These findings provide an empirical basis for developing more targeted and effective infant health policies.
EDUKASI DAN PELATIHAN PENGOLAHAN MINUMAN IMUNOMODULATOR PADA PENDERITA TB PARU DI WILAYAH PUSKESMAS CEMPAKA BANJARBARU Dewi Dwiyanti, Ratih; Nurlailah, Nurlailah; Insana, Aima
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 5 No 1 (2026): Jurnal Rakat Sehat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v5i1.90

Abstract

Pulmonary TB is still a disease that is often found in the Cempaka Banjarbaru Community Health Center. Based on data from the Cempaka Health Center for January - December 2022, there are 36 tuberculosis sufferers who are still actively seeking treatment. Treatment of pulmonary TB with anti-tuberculosis drugs (OAT) takes several months so it is prone to causing side effects in sufferers. To minimize the side effects of using OAT while increasing the patient's immune system, it is necessary to consume natural ingredients (moringa, meniran and ginger) which have minimal side effects and act as immunomodulators. The aim of the activity is to increase participants' knowledge about tuberculosis and how it is transmitted as well as how to use herbal plants as immunomodulators to minimize side effects due to the use of OAT. The method used in this activity is filling out a questionnaire sheet (pretest) followed by a presentation of the material. Before the posttest was carried out, the participants were given training on how to process herbal drinks from 3 herbal ingredients, namely Moringa leaves, meniran and ginger (Kemeja) which were made in powder form for 2 hours and continued with filling out a questionnaire sheet (posttest). The results of community service can be seen based on the questionnaire sheet which shows an increase in participants' knowledge about pulmonary TB disease by 10% and behavior in preventing pulmonary TB by 10% and participants were able to make immunomodulatory drinks in the form of powder such as Kemeja (moringa, meniran, ginger). Based on the results of evaluation and monitoring, sufferers stated that their appetite increased after drinking SHIRT tea powder. It is hoped that the increase in appetite can increase the sufferer's immune system so that they can suppress the side effects of the OAT drugs they consume.
EDUKASI DAN KESADARAN MASYARAKAT TENTANG PENYAKIT JAMUR KUKU PADA PENDULANG INTAN DI CEMPAKA KOTA MADYA BANJARBARU Dewi Dwiyanti, Ratih; Lutpiatina, Leka; Insana, Aima
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 5 No 1 (2026): Jurnal Rakat Sehat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v5i1.124

Abstract

Diamond miners are one type of job that deals daily with wet and dirty places. Diamond miners work while wearing boots, but because the mining areas are filled with water, their feet are always damp and submerged in dirty water, which supports the growth of fungus. The objective of this activity is to enhance community health knowledge about fungal nail diseases and to reduce the incidence of fungal infections by providing antifungal medication to diamond miners in the area of the Tiung River in Cempaka, Banjarbaru. The methods used in this activity included pre-tests, presentation of materials, observation, post-tests, and the administration of antifungal medication. This activity involved 29 participants consisting of 5 females and 24 males. The results of the community service showed increased knowledge among partners regarding fungal infections of the nails based on the questionnaire results. As a result of the observation, it was found that all participants showed symptoms of fungal infection in their toe nails. Monitoring and evaluation were conducted 3 weeks after the counseling and observation activities, during which antifungal medication was distributed. It was concluded that the counseling activities could enhance the knowledge of the diamond miners
Co-Authors Ahmad Muhlisin Ahmad Muhlisin akhmad muntaha Erfan Roebiakto, Erfan Fransiskus Xaverius Agung Pinto Laksono Habibah, Siti Noor Hafidh, Muhammad Ipniliati, Eva Khairir Rizani, Khairir leka lutpiatina, leka Meilla Dwi Andrestian, Meilla Dwi Mufid, Muhammad MUHAMMAD RIZAL Naning Kisworo Utami Neni Oktiyani Noraini Noraini, Noraini Nur Ain Mirandayanti Nurlailah Nurlailah Pinto Laksono, Fransiskus Xaverius Agung Putri Wulandari, Rima Putri, Noorlaily Rabiatul Adawiyah Ratih Dewi Dwiyanti Rifqoh Rifqoh Rifqoh Rifqoh Rifqoh, Rifqoh Rima Putri Wulandari Rizkiyah, Risa Rizky Arsyanti Rumiyati Sari, Endah Permata SATRIYAS ILYAS Siti Rachmah Ulfah, Putriana Utama, Ricko Dharmadi wahdah norsiah Wahdah Norsiah, Wahdah Wulandari, Eka Dwi