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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL BIOLOGI INDONESIA JFMR (Journal of Fisheries and Marine Research) Indonesian Aquaculture Journal Media Akuakultur Journal of Tropical Marine Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Ni Wayan Widya Astuti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Library & Information Science

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Pengaruh Pemberian Pakan Kombinasi Nannochloropsis oculata Dan Terasi Dengan Dosis Yang Berbeda Terhadap Laju Pertumbuhan Rotifera (Branchionus Plicatilis) Paramitha, Komang Putri Regina; Amelia, Jasmine Masyitha; Fain, Hamdanul; Astuti, Ni Wayan Widya
Journal of Tropical Marine Science Vol 7 No 2 (2024): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v7i2.4150

Abstract

Penelitian yang dilakukan bertujuan untuk mengetahui pengaruh kombinasi pakan Chlorella dan terasi yang berbeda terhadap laju pertumbuhan populasi rotifera (Branchiounus plicatilis). Penelitian dilakukan dari bulan Maret sampai dengan bulan Mei 2023 di Kawasan Konservasi Ilmiah Biota Laut Badan Riset dan Inovasi Nasional Gondol Bali. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan A sebagai kontrol hanya diberikan pakan Chlorella sebanyak 6,66 x 105 sel/ml perlakuan B yakni Chlorella 6,66 x 105 sel/ml dan terasi 1gr/L. perlakuan C yakni Chlorella 6,66 x 105 sel/ml dan terasi 2gr/L, dan perlakuan D yakni Chlorella 6,66 x 105 sel/ml dan terasi 3gr/L. dengan dosis pemberian pakan kombinasi dilakukan setiap 2 hari sekali. Parameter yang dianalisis hanya laju pertumbuhan populasi dan mencari dosis yang terbaik. Data di olah menggunakan One Way ANOVA dengan tingkat kepercayaan 95% dan jika hasil menunjukkan berbeda signifikan maka dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa pertumbuhan populasi Rotifera meningkat dengan pemberian kombinasi pakan Chlorella ditambah terasi dengan dosis 1gr/L. pada perlakuan B pertumbuhan populasi memiliki puncak kepadatan sebanyak 53 ind/ml dengan laju pertumbuhan 0,8741 ind/ml/hari. Jadi dosis terasi terbaik yang diberikan untuk kultur rotifera sebanyak 1gr/L.
CHARACTERZATION OF ENDOGENOUS BACTERIA AS POTENTIAL PROBIOTICS IN LARVAL REARING OF YELLOWFIN TUNA (Thunnus albacares) Astuti, Ni Wayan Widya; Setiadi, Ananto; Gunawan, Gunawan; Hutapea, Jhon Harianto; Muzaki, Ahmad; Haryanti, Haryanti
Indonesian Aquaculture Journal Vol 20, No 2 (2025): (December, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.2.2025.209-220

Abstract

The main objective of this study was to determine the profile of endogenous intestinal bacteria of yellowfin tuna, Thunnus albacares that can function as potential probiotics in the digestive system and used for the maintenance of yellowfin tuna larvae. Wild yellowfin tuna, T. albacares was collected from the waters of Gerokgak, Buleleng, North Bali, Indonesia. Intestinal bacteria were isolated, identified, characterized, and followed by enzymatic hydrolysis activity tests, antagonistic tests and pathogenicity tests to select candidate bacteria as probiotics. The results of the enzymatic hydrolysis activity test showed that there were 4 isolates of candidate probiotic bacteria that were active in enzymatic synthesis for amylase, lipase, casease, lecithinase, gelatinase. Identification through Sanger sequencing resulted in 4 potential bacterial isolates as probiotics, namely Bacillus subtillis strain T-A1, Bacillus amyloliquefaciens strain T-N2, Bacillus subtillis strain T-O4, and Paraclostridium bifermentans strain T-O6. These four bacteria were not antagonistic between varieties and were not pathogenic in Tuna fish. Yellowfin tuna fed probiotic supplements grew faster (5.5002 ± 0.2 mm) than the control (4.9002 ± 0.4 mm). The success of yellowfin tuna seed production is expected to be sustainable and its implementation in the private sector for aquaculture purposes. 
The use of technical chlorine for seawater sterilization in the rearing of barramundi larvae Lates calcarifer Ni Wayan Widya Astuti; Indah Mastuti; I Made Sedana; Sunarto Sunarto; Mustakim Mustakim; I Nyoman Suwitra; Mujiono Mujiono; Kurdi Kurdi; Luh Yuliani Dewi; Kadek Mas Tantra; Katimin Katimin; Ja'far Shadiq; Ahmad Muzaki
Depik 2025: Special Issue ICMF
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46920

Abstract

Disease prevention is very important in the rearing of barramundi larvae, one of which is by sterilizing the seawater using technical chlorine. This study aimed to determine the effect of seawater sterlization use technical chlorine for rearing barramundi larvae on growth, survival, and water quality. The treatment in this study was different seawater used for water exchange during larval rearing. Seawater was sterilized with 25 ppm technical chlorine for 24 hours. Before use, the water was neutralized using 12.5 ppm sodium thiosulfate. As a control in this study, direct seawater or untreated sea water was used for water exchange. The study was conducted with 3 replications. The parameters observed in this study were growth, survival, virus monitoring, growth variation, and water quality. Sterilization of seawater with 25 ppm technical chlorine used for rearing larvae of barramundi has no significant effect on growth, survival, growth variation and water quality of rearing larvae. The use of filtered seawater is recommended in the sterilization of seawater for rearing larvae of barramundi. Increasing the dose of chlorine or using chlorine which has a high active substance could increase effectiveness of water sterilization.Keywords:Technical chlorineSterilizationRearing larvaeBarramundiLates calcarifer
Co-Authors Afifah Nasukha Ahmad Muzaki Ahmad Muzaki Ansari, Mohamad Ariani, Ni Kadek Bejo Slamet Dewi, Luh Yuliani Fain, Hamdanul Gunawan Gunawan Haryanti Haryanti Haryanto, Slamet I Made Sedana I Nyoman Suwitra Indah Mastuti Ja'far Shadiq Jasmine Masyitha Amelia Jhon Harianto Hutapea Kadek Mas Tantra Katimin Katimin Katimin, Katimin Ketut Mahardika Ketut Suwirya Kurdi Kurdi Kurdi Kurdi Luh Yuliani Dewi Mastuti, Indah Miniartini, Made Muhammad Marzuqi Muhammad Marzuqi Mujiono Mujiono Mujiono Mujiono Mustakim Mustakim Mustakim Mustakim Mustaqim Mustaqim, Mustaqim Nyoman Adiasmara Giri Paramitha, Komang Putri Regina Rarastoeti Pratiwi Regina Melianawati Regina Melianawati Sadewa, Ketut M. Arya Sedana, I Made Setiadi, Ananto Shadiq, Ja'far Suko Ismi Suko Ismi Sunarto Sunarto Sunarto Sunarto Sunarto, Mujiono Suwitra, I Nyoman Tantra, Kadek Mas Yasmina Nirmala Asih zailani, Ahmad