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Keragaman dan Daya Hambat Spora Tular Udara yang Mengkontaminasi Media Baglog Jamur Tiram (Pleurotus ostreatus (Jacq. ex Rr) Kummer) I MADE SUDARMA; NI MADE PUSPAWATI; NI NENGAH DARMIATI; KETUT AYU YULIADHI; NI WAYAN SUNITI; I GUSTI NGURAH BAGUS; I NYOMAN WIJAYA; DWI WIDANINGSIH
Agrotrop : Journal on Agriculture Science Vol 5 No 2 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Diveristy and Inhibition of Air-Borne Spores That Contaminate Substrat of OysterMushroom (Pleurotus ostreatus (Jacq. Ex Fr) Kummer). The aim of research to know thediversity, prevalence and inhibition of air-borne spores that could potentially contaminatesubtsrat baglog of oyster mushrooms. In this study using the method: (1) the arrest of sporescarried out during the hours of 7 am to 13 pm by placing three petri dish that already containsmedia PDA 3 pieces, and repeated 3 times, (2) inhibition ability of air-borne spores againstoyster mushroom. The place and time of the study conducted at Jl. Siulan Gang Zella No. 7Denpasar, and implemented in October 2014 to February 2015. The results showed that 13genera, including Aspergillus spp, as many as 10 with a prevalence of 13.51%, Aspergillusniger 7 (9.45%), Brachysporium sp., 1 (1.35%) , Cunninghamella sp. 1 (1.35 %), Fusariumspp. as many as 19 (25.6 %), Giotrichum sp. 1 (1.35%), Mucor spp. , a total of 18 (24.32%),Neurospora spp., as many as 8 (10.81%), Penicillium spp. 2 (2.70%), Phytophthora spp. 4(5.40%), Stachybotrys sp. 1 (1.35%), Trichoderma sp. 1 (1.35%) and Umbelopsis sp. 1 (1.35%). The highest prevalence achieved by Fusarium spp . amounting to 25.67 %, followed byMucor spp. 24.32%. Diversity index (H') was obtained from each replicate of 0.6438, 0.7048,0.5611 with a 0.6366 average. Dominance index (C) obtained by 0.6331. 0.8646, 0.6146, andaverage 0.7041. Diversity including < 1, marked by low diversity and dominance index closeto 1, meaning there is dominance. The dominance held by Fusarium spp . and Mucor spp.Inhibitory effects of air -borne fungi on the growth Oyster mushroom in vitro; The highestachieved by Fusarium spp, with inhibition of 94.00 ± 1.2%, followed by Aspergillus spp.amounting to 92.15 ± 1.5%, and the lowest was achieved by Penicillium spp . amounting to70.37 ± 2.5%.
Parameter Biologi dan Demografi Parasitoid Diadegma semiclausum Hellen (Hymenoptera : Ichneumonidae) pada Plutella xylostella L. (Lepidoptera : Plutellidae) NI PUTU NIA KUMARAWATI; I WAYAN SUPARTHA; KETUT AYU YULIADHI
Agrotrop : Journal on Agriculture Science Vol 8 No 2 (2018)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.45 KB) | DOI: 10.24843/AJoAS.2018.v08.i02.p06

Abstract

Biological and Demographic Parameters of Parasitoid Diadegma semiclausum Hellen (Hymenoptera: Ichneumonidae) on Plutella xylostella L. (Lepidoptera: Plutellidae). Diadegma semiclausum Hellen (Hymenoptera: Ichneumonidae) is one of the parasitoid larvae which attacks Plutella xylostella (Lepidoptera: Plutellidae) effectively. This research was aimed to know the biology of parasitoid D. semiclausum in broccoli. The research was carried out in a laboratory by observing the development of parasitoid, fecundity, longevity the males and females as well as parameters demographic of D. semiclausum. The results showed that immature development time of D. semiclausum parasitoid from egg up to adult the imago was 10.65 ± 0.63 days, with the number of fecundity was 22.9 grains. Longevity of male imago of D. semiclausum was 12.10 ± 0.74 days and imago of females 9.80 ± 1.87 days. Parameters demographic of D. semiclausum showed that net reproduction rate (R0) was 22.11 per generation, intrinsic growth rate (r) was 0.18 per day, limited increased rate (?) was 1.16 individuals per day, generation time (T) was 17.50 day.
Hubungan Antara Keragaman Gulma dengan Penyakit Bulai pada Jagung (Zea mays L.) Stadium Pertumbuhan Vegetatif I MADE SUDARMA; I KETUT SUADA; KETUT AYU YULIADHI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Weeds play an important role in the spread of downy mildew disease, competition, and lower cropyields of corn. Research using the survey method in 3 locations of Sanur village, Denpasar City. Eachlocation is taken 5 samples, and each sample area was 1m2, which was arranged diagonally. Forweeds identification was using reference i.e. Weeds of rice in Indonesia, and identification of downymildew disease using reference i.e. Maize Disease: A Guide for Field Indentification and microscopically,while the weeds diversity was determined based on Diversity Index of Shannon-Wiener. Relationshipbetween number of weed species and weed populations, and disease incidence were determined byregression and correlation analysis. Weed diversity on ecosystem of maize in vegetative stage is verylow with the diversity index of 0.779, this means weed ecosystem unstable, and dominated by a particularweed species was Paspalum commersonii Lamk. (Fam. Poaceae/Graminaceae) of 23.3%, with thedominance index of 0.709. Relationship between the number of weed species and disease incidence ofthe downy mildew disease was highly significant (P<0,01), while the relationship between weed populationand disease incidence of the downy mildew disease is not significant. Plant height and leaf number ofmaize was influenced by the type of weed, but weed populations only affect plant height of maize invegetative stage (V3-V11).
Komposisi Spesies Penggerek Batang Padi dan Parasitoid Telurnya pada Ketinggian Tempat yang Berbeda I NYOMAN WIJAYA; WAYAN ADIARTAYASA; KETUT AYU YULIADHI
Agrotrop : Journal on Agriculture Science Vol 11 No 1 (2021)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2021.v11.i01.p01

Abstract

Composition of Rice Stem Borer Species and Their Egg Parasitoids at Different Altitudes. Rice stem borer is one of the main pest of rice plants that always percolating and attack plants in the field. The pests can result in damage to plants and lost of the grain from season to season. The aim of this research is to know composition and dominance rice stem borer species and role of their eggs parasitoid at an altitude of different places. Research is done by survey based on the height of different altitude in rice plants two weeks after cropping up until eleven weeks after planting. Research conducted from April until August 2019 in rice field that belonging to farmers at Subak Cemagi Let, Cemagi Village and Subak Teba, Mengwi village, Badung regency, Bali province. Subak Cemagi Let are at an altitude of 15 meters above sea level whereas Subak Teba are at an altitude of 300 meters above sea level. Identification of Rice stem borer larvae and their eggs parasitoid done in Pest Laboratory, Agriculture Faculty of Udayana University. Found three species rice stem borer in the height 15 mdpl namely Scirpophaga incertulas; Sesamia inferens dan Chilo suppressalis, whereas in the height 300 mdpl found four species namely Scirpophaga incertulas, Sesamia inferens, Chilo suppressalis, dan Chilo polychrysus. S. incertulas was the most dominant at the two different altitude. Three species of eggs parasitoid that have role to suppress the development of rice stem borer at the different altitude namely Tetrastichus schoenobii, Telenomus rowani dan Trichogramma japonicum. T. schoenobii was the most dominant to suppress rice stem borer populations.
Dinamika Populasi Diaphorina citri Kuwayama (Homoptera: Psyllidae) dan Deteksi CVPD dengan Teknik PCR I NYOMAN WIJAYA; WAYAN ADIARTAYASA; MADE SRITAMIN; KETUT AYU YULIADHI
Jurnal Entomologi Indonesia Vol 7 No 2 (2010): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.745 KB) | DOI: 10.5994/jei.7.2.78

Abstract

The research was conducted in the field of Taro village Gianyar regency and Katung village Bangli regency and at the Laboratory of Biotechnology Faculty of Agricultural Udayana University. The purpose of the research were to investigate population dynamic of Diaphorina citri Kuwayama and other insects that were assosiated with citrus cv. siam; and molecular detection of CVPD patogen in its vector, other phytophagous insects on citrus cv.siam and kemuning [Murraya paniculata (L.) Jack.] The result of this study showed that in six month period there was one peak of D. citri population which was associated with the flushing period of citrus at Taro as well Katung villages. The peak of D. citri population of Taro village was higher than that of Katung village. Two species of parasitoids and four spesies of predators were found in the field and may contribute in the suppression of D. citri population. The parasitoids identified were Tamarixia radiata Wat. and Diaphorencyrtus alligharensi Shaffe, whereas the predators were Curinus coeruleus Mulsant, Phiddipus sp., Menochilus sexmaculatus Fabricius and Oxyopes javanus Thorell. T. radiata has been proven to be an effective nymph parasitoid that could suppress the population development of D. citri in citrus orchads. The research revealed that kemuning could serve as a source of infection because it is also a host of CVPD disease as well as of D. citri. Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. were not proven to contain CVPD disease.
MtCOI DNA sequences from Sycanus aurantiacus (Hemiptera: Heteroptera: Reduviidae) provide evidence of a possible new harpactorine species from Bali, Indonesia I Putu Sudiarta; Dewa Gede Wiryangga Selangga; Gusti Ngurah Alit Susanta Wirya; Ketut Ayu Yuliadhi; I Wayan Susila; Ketut Sumiartha
Jurnal Entomologi Indonesia Vol 18 No 1 (2021): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.18.1.74

Abstract

Sycanus aurantiacus Ishikawa & Okajima, found in Bali, was first described in 2007 as a new harpactorine species based on morphological and biological characteristics; however, its genome has not yet been sequenced. In this study, we examine the mitochondrial cytochrome oxidase subunit I (MtCOI) nucleotide sequence of S. aurantiacus in order to determine whether it represents a new harpactorine species. A sample from Pancasari, Bali, Indonesia was collected at the same location S. aurantiacus was first discovered in 2007. The selected mtCOI gene (650 bp) was successfully amplified using mtCOI primer pairs LCO1490 and HCO2198, and the resulting MtCOI sequence of the S. aurantiacus sample was compared with those from other hapactorine species recorded in GenBank. This comparison revealed low genetic similarity between S. aurantiacus and most other harpactorine species worldwide, except for the Genus Sycanus (JQ888697) from USA whose mtCOI shares approximately 91% similarity with the Pancasari sample. Phylogenetic analysis indicated a close genetic relationship between Sycanus from Bali and the Genus Sycanus (JQ888697) from the USA. The mtCOI sequence of S. aurantiacus had not been recorded previously, and our comparison with existing Sycanus sequences provides support to the understanding that S. aurantiacus is indeed its own species.
Insidensi Serangan dan Perkembangan Populasi Hama Invasif, Spodoptera frugiperda (J. E Smith) (Lepidoptera: Noctuidae) pada Tanaman Jagung dan Sorgum di Bali CRISTINE LAURINE SIMANJUNTAK; I KETUT SUMIARTHA; KETUT AYU YULIADHI; I WAYAN SUPARTHA
Agrotrop : Journal on Agriculture Science Vol 12 No 1 (2022)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2022.v12.i01.p01

Abstract

Incidence of Attacks and Population Development of Invasive Pests, Spodoptera frugiperda (J.E Smith) (Lepidoptera: Noctuidae) on Corn and Sorghum Crops in Bali. Spodoptera frugiperda is known as an invasive pest which now become important pest on corn crops in Indonesia and Bali in particular, but in this study the Spodoptera frugiperda was also found to attack sorghum. This study used purposive survey method and on land that had been fixed. Plant sampling was carried out in all regencies of Bali Province by means of diagonal sampling. The size of unit sample was 2 x 2m and the number of plants observed was 20 plants. The results of this study showed that the incidence of S. frugiperda attack has been observed on plant beds in all districts/cities in Bali which is indicated by symptoms of attack and abundance of S. frugiperda population on corn and sorghum in the field. The highest abundance of S. frugiperda larvae population was in sweet corn in Klungkung Regency as much as 38.67 heads, and the lowest was in sorghum numbu in Tabanan Regency as many as 27.00 individuals per observation plot. Population development of egg groups was found in plants aged 1 (MST) while larvae of S. frugiperda showed the same pattern in sweet corn, sticky rice and pipil plants and the peak of larval population occurred in plants aged 4 WATS respectively. While the peak population of larvae in sorghum plants occurred at 5 WAP and imago populations in sweet corn were found in sweet corn plants at 2 WAP and glutinous corn at 4 WAP. The highest percentage of S. frugiperda attack was on sweet corn with an average attack of 43% per observation plot and the lowest on sorghum numbu with an average of 25% per observation plot.
Induced Mutagenesis in Yellow Flowering Marigold with Colchicine in Hydrogen Peroxide I Gede Ketut Susrama; Ketut Ayu Yuliadhi
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 2 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.478 KB) | DOI: 10.24843/ATBES.2020.v04.i02.p04

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Marigold flower has many benefits in many aspects of life in Bali Island (Indonesia) including often used in religious ceremonial activities. For controlling plant pests and diseases, marigold is planted on the sidelines or on the edge of a land as an attractant crop to invite natural enemies and marigold also can produce certain substance which can reduce the incidence of nematode. In inducing mutagenesis in this research, the seeds of marigold were soaked in solution of colchicine with hydrogen peroxide as its solvent. The results of this research indicated that the treatments caused the formation of chlorophyll mutant and an increase in the length of flower diameter. Both the control and treated plant samples were attacked by leaf miner, giant land snail, virus-like disease and botrytis flower blight disease.
Presentase dan Intensitas Serangan Hama Kutu Putih (Paracoccuss marginatus) yang Menyerang Tanaman Adenium Spp. di Kota Denpasar I Wayan Andi Sumartayasa; Ketut Ayu Yuliadhi; I Ketut Sumiartha
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Research on the Percentage and Intensity of Attacks by White flies (Paracoccuss marginatus) Attacking Adenium Spp. in Denpasar City. The purpose of this study was to determine the White flies (Paracoccuss marginatus) that attacks Adenium spp., the population of pests that attack, the percentage and intensity of attacks. Sampling was carried out purposively by photographing and observing plants attacked by pests in the Districts of West Denpasar, East Denpasar, South Denpasar and North Denpasar. The results showed that White flies (Paracoccuss marginatus) attacked Adenium spp. in the city of Denpasar. White flies (Paracoccuss marginatus) number 5468 pests, with an average attack percentage of 56.25% White flies, while the average pest attack intensity is 20% White flies.
Molecular characterization of Rice ragged stunt virus and Rice grassy stunt virus on Rice in Gianyar, Bali, Indonesia Listihani, Listihani; Selangga, Dewa Gede Wiryangga; Yuliadhi, Ketut Ayu; Yuniti, I Gusti Ayu Diah; Ariati, Putu Eka Pasmidi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12448-57

Abstract

Rice stunt disease is one of the causes of rice harvest failure. It is caused by the infection of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) infection. Information about disease severity and the molecular characteristics of stunt viruses in Indonesia is still limited. Thus, this research aimed to determine the disease severity and the genetic diversity of rice stunt viruses in Gianyar, Bali. The research method consisted of observation of incidence and disease severity in the field and virus detection by reverse transcription-polymerase chain reaction (RT-PCR) using primers specific for RRSV and RGSV. The observation of the disease incidence and severity were performed in seven districts in Gianyar Regency, Bali, namely Blahbatuh, Gianyar, Payangan, Sukawati, Tampaksiring, Tegallalang, and Ubud. Stunt disease was found in all observation sites. High stunt disease incidence (> 44%) was found in three districts: Ubud, Tampaksiring, and Payangan, while the low disease incidence rate of <10% was found in Blahbatuh and Gianyar Districts. The highest stunt disease severity occurred in Tampaksiring District (60.82%), while the lowest severity occurred in Gianyar District (18.84%). The IR-64 and Ciherang cultivars are vulnerable to rice stunt disease infection. The highest homology of RRSV and RRGV nucleotides was found with Vietnam isolates being >98% and >97%, respectively. The phylogenetic analysis showed that Indonesian isolates of RRSV and RGSV were clustered in the same group as Vietnam isolates.
Co-Authors ADNYANA, I MADE MEGA AGUS PUTRA SEDANA Anak Agung Ayu Agung Sri Sunari ANAK AGUNG GDE AGUNG NARENDRA ARIYANTA, I PUTU BAWA Bakar, Nor Kartini Abu CATUR KOESUMA WARDHIANY Cha, Joo Young Costa Cruz, Latizio Beni Da CRISTINE LAURINE SIMANJUNTAK Devi, Komang Saraswati Devi, Putu Shinta Devitriyani, Ida Ayu Dewa Gede Wiryangga Selangga Dinarkaya, Shah Mahapati DWI WIDANINGSIH GARGITA, I WAYAN DIKSA GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya Husni dan Zulfanazli Pudjianto Alfian Rusdy I Dewa Nyoman Nyana I G. R. M. TEMAJA I GEDE AGUS ADI WIRADARMA I Gede Ketut Susrama I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I Ketut Suada I KETUT SUMIARTHA I MADE SUDARMA I MADE SUDARMA I NYOMAN WIJAYA I Putu Sudiarta I Wayan Andi Sumartayasa I Wayan Supartha I Wayan Susila IDA BAGUS GDE PRANATAYANA IDA BAGUS GEDE MAHENDRA Klett, Katrina KORI’ ANGGRAINI Listihani, Listihani MADE SRITAMIN Mariana, Santi MERTA, I NGURAH MEGA MIFTAHUS SIROJUDDIN Mintari, Gusti Ayu Willem NI KADEK BUDARTINI NI KADEK VENIARI NI KETUT JULI DWI JAYANTI Ni Made Puspawati NI NENGAH DARMIATI NI PUTU ESA YANTI SUPARTHA NI PUTU NIA KUMARAWATI NI PUTU NIA KUMARAWATI NI WAYAN SUNITI Ni Wayan Suryati Agustini Parwata, I Made Oka A PRATIWI, NI PUTU EKA Purnomo Purnomo Putu Eka Pasmidi Ariati Putu Perdana Kusuma Wiguna SANG AYU MADE INTAN PUTRI RAHAYU Santi Mariana Selangga, Dewa Gede Wiryangga SUGIARTA, DWI Sumartayasa, I Wayan Andi SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA WAYAN ADIARTAYASA YUDHA, I KADEK WISMA