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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Agrovigor: Jurnal Agroekoteknologi Journal of Tropical Biodiversity and Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Advances in Tropical Biodiversity and Environmental Sciences Agrica: Journal of Sustainable Dryland Agriculture Nandur
KETUT AYU YULIADHI
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Veterinary

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Kombinasi Penggunaan Urin Kelinci dan Ekstrak Daun Pepaya (Carica papaya L.) terhadap Kelimpahan Hama pada Tanaman Pakcoy (Brassica rapa L.) Devitriyani, Ida Ayu; Adnyana, I Made Mega; Yuliadhi, Ketut Ayu
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p12

Abstract

Combination of Rabbit Urine and Papaya Leaf Extract (Carica Papaya L.) Usage on Pest Abundance on Pakcoy Plants (Brassica rapa L.). Pak coy (Brassica rapa L.) is a vegetable that are rich in nutrients. According to the Central Statistics Agency (2021) the production of Brasicaceae in Bali from 2018 to 2020 has been fluctuated. One of the factor’s hindering productions is the existence of pest attacks. This study aims to determine the effect of a combination of rabbit urine and papaya leaf extract on the types of pests, pest attack intensity, diversity and abundance of pests that attack pak coy plants in Penebel, Tabanan, Bali. The research held in July 2022 – November 2022 with a factorial Randomized Block Design (RBD) of 2 factors, the first factor was rabbit urine and papaya leaf extract and the second factor was concentration. Rabbit urine consisted of 4 concentrations (K0: 0 ml/l water, K1: 20 ml/l water, K2: 40 ml/l water, K3: 60 ml/l water) and papaya leaf extract consisted of 3 concentrations (P0: 0 ml/l water, P1: 400 ml/l water, P2: 600 ml/l water). The results showed that there were four types of pest species attacked pakcoy plants namely Atractomorpha crenulata (75 individuals), Oedaleus infernalis (63 individuals), Plutella xylostella (57 individuals), and the least was Vaginula bleekeri (21 individuals). The effective combinations of rabbit urine and papaya leaf extract to reduced the intensity attacks of pest was rabbit urine 60 ml/l water with papaya leaf extract 600 ml/l water (K3P2) of 7.4%, the highest damage showed by the control treatment (K0P0) of 70%. The index diversity species of pest found in all treatments was moderate. The lowest abundance of pest species was found in the K3P2 treatment (4 individuals), the highest in the K0P0 treatment (55 individuals). There was not found interaction effect between rabbit urine and papaya leaf extract.
First Report of Cladosporium dominicanum Zalar, de Hoog & Gunde-Cim. Infecting Whitefly on Ornamental Plants in Bali, Indonesia Sudiarta, I Putu; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Gargita, I Wayan Diksa; Yuliadhi, Ketut Ayu; Klett, Katrina
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86865

Abstract

As natural enemies, entomopathogenic fungi are essential for controlling certain plant pests, such as whitefly. Many types of entomopathogenic fungi can infect whiteflies, including those from the genera Aschersonia, Metarhizium, Beauveria, and Cladosporium. Currently, there is great interest in using entomopathogenic fungi as an environmentally friendly pest control in organic and sustainable agricultural systems. This study aimed to identify entomopathogenic fungus associated with whiteflies. Identification of entomopathogenic fungus was performed morphologically and molecularly using DNA barcoding with ITS-1 and ITS-4 as primers, in addition to sequencing and phylogenetic tree analysis. Whiteflies infected with entomopathogenic fungus were discovered on ornamental plants (Premna serratifolia, Ficus religiosa, and Ficus rumphii) in Denpasar, Bali, Indonesia from January to March 2023. We found that samples from the field had mycelium that was unbranched or sparingly branched with solitary conidiophores arising terminally from ascending hyphae or laterally from plagiotropous hyphae. Samples from PDA media had ramoconidia with darkened septa, straight and conidia in long branched chains, branching in all directions, conidia were obovoid, ovoid to limoniform. The fungal colonies had the characteristics of being grey-olivaceous, olivaceous-black, and the margins were grey-livaceous to white, gray olivaceous in colony center due to abundant sporulation. In addition, based on the BLAST nucleotide sequence from NCBI GenBank, the insect pathogen phylogenetic tree found in Denpasar, Bali, is in the same clade as Cladosporium dominicanum strain SCAU014, accession number KY827344.1. Based on morphological and molecular analysis the fungus that infects whiteflies on ornamental plants is C. dominicanum.
Occurrence of Cassava Lace Bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) in Bali, Indonesia Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Devi, Komang Saraswati; Ariyanta, I Putu Bawa; Wirya, Gusti Ngurah Alit Susanta; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Gargita, I Wayan Diksa; Wiguna, Putu Perdana Kusuma; Yuliadhi, Ketut Ayu; Devi, Putu Shinta
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87438

Abstract

Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava. 
Phytochemical Content Identification And Antioxidant Activity Test Of Ethanol Extract Parijata Fruit (Medinilla Speciosa Blume) In Bedugul Area, Bali, Indonesia Mintari, Gusti Ayu Willem; Suada, I Ketut; Phabiola, Trisna Agung; Wijaya, Nyoman; Yuliadhi, Ketut Ayu; Bakar, Nor Kartini Abu
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p04

Abstract

Antioxidants are compound that function as free radical scavengers, these compounds can be found in parijata fruit (Medinilla speciosa Blume). The secondary metabolites of this fruit can be identified by the GC-MS method. The aims of study to identification of phytochemical content and test the antioxidant activity of the ethanol extract of Parijata fruit (Medinilla speciosa Blume) in the Bedugul area of Bali. This method is used because it is more sensitive and the results of the analysis are easy to understand. Using the GC-MS method, 11 organic compounds with a quality value of ? 90 were found in the ethanol extract of parijata fruit which have antioxidant, antimicrobial, and others. The 3 compounds with the highest Area Under Curve were 5-Hydroxymethylfurfural (AUC 31.05%); 1,2,3- Benzenetriol (AUC 22.32%); and 4H-pyran-4-one-2,3-dihydro-3,5-dihydroxy-6-methyl- (AUC 6.97%). These three compounds have antioxidant properties. Parijata fruit antioxidant activity test was tested with the DPPH method. The antioxidant activity of the ethanol extract of parijata fruit has an IC50 value of 11.26 mg/L, in the category of very strong antioxidant activity. So that with this category, parijata fruit can be given further treatment as food for health. In addition, compounds with antimicrobial properties can be used as biopesticide products in agriculture.
The Role of Egg Parasitoids to Control Rice Stem Borers (Scirpophaga incertulas) Walker (Lepidoptera: Pyralidae) in Subak Renon, Denpasar-Bali, Indonesia Yuliadhi, Ketut Ayu; Wijaya, I Nyoman; Costa Cruz, Latizio Beni Da; Yudha, I Kadek Wisma; Utama, I Wayan Eka Karya; Cha, Joo Young
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p02

Abstract

Rice stem borers are the main pest and endemic for the rice plants. The intensity of the attacks paneled fluctuating until it Reaches 90% and causes loss and it is requisite to get to pay serious attention. One of Efforts to Overcome it is the implementation of integrated pest management (IPM) through biological control by natural competitors. Research objectives is to know the role of an egg parasitoids in controlling rice yellow stem borer. Research conducted at Subak Renon, Renon Village, South Denpasar District, Denpasar Regency since April to July 2022. Research is done with the survey by truncate leaf rice containing eggs of rice stem borers at random. The sampling done every week since the plants were two weeks old in Ciherang rice plants. Identification of eggs parasitoids observed under microscope used the key determination insects (Kalshoven, 1981) and the percentage of eggs parasitoids is calculated by a formula Nishida and Torri (1970). The result showed that found three species of parasitoid eggs roomates played the role to suppress the development of yellow rice stem borer namely Trichogramma japonicum Ashm., Telenomus rowani Gahan and Tetrastichus schoenobii Ferr with parasitation capacity of 66%. The highest capacity parasitation played by T. japonicum, followed T. rowani and T. schoenobii Ferr.
Inventory of pests attacking Adenium spp. in Denpasar city Gargita, I Wayan Diksa; Sudiarta, I Putu; Yuliadhi, Ketut Ayu; Sumartayasa, I Wayan Andi
Agrovigor Vol 17, No 2 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i2.24176

Abstract

The cultivation of Adenium spp. encountered several challenges involving physical, environmental, and plant pest interference factors. Inventory of pests aimed to find out the kind of pests, the symptoms of the attacks, and the percentage of the pests that attack Adenium spp. Samples were taken using a purposive method by taking pictures and observing plants attacked by pests in 4 sub-districts in Denpasar, namely West Denpasar, East Denpasar, South Denpasar, and North Denpasar. The research results show that several pests attack Adenium spp. in Denpasar City, including mealybugs, spider mite, red cotton bug, and aphids. The study's findings indicate that Adenium spp. in Denpasar City are susceptible to several pests, such as mealybugs, spider mite, red cotton bug, and aphids. These organisms induce the dehydration, withering, and stunted growth of plants due to fluid loss. Mealybugs exhibit the highest incidence of infestation, with a prevalence rate of 85%. Spider mites follow with an infestation rate of 50%, while red cotton bug and aphids display infestation rates below 50%. The presence of several pest insects in Adenium spp. is indicative, with certain pests posing a significant threat to the plants, perhaps resulting in severe damage or even plant mortality. This research can provide additional information of pests that attack ornamental plants so that the strategy of pest control on ornamental plants, especially Adenium, can be developed.
Bio-attractant innovation with nano-gel technology to detect and control Silba adipata McAlpine on white cayenne peppers in Bali, Indonesia Merta, I Ngurah Mega; Supartha, I Wayan; Susila, I Wayan; Parwata, I Made Oka A; Yuliadhi, Ketut Ayu; Purnomo, Purnomo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12517-31

Abstract

Silba adipata is a new pest that attacks white chili pepper in Bali, Indonesia. Most farmers control this pest with synthetic insecticides although this method poses significant risks to human health and the environment. Therefore, innovations and control technologies that are environmentally friendly and sustainable are urgently needed. Preliminary results show that S. adipata frequently attacks local figs (Ficus variegata) in Bali. Dried figs (Ficus carica) have been reported to effectively attract S. adipata adults. This study aims to (1) determine the phytochemical compounds in F. variegata fruit extracts that have the potential to act as attractants for S. adipata during the process of searching for and locating host plants; (2) evaluate the effectiveness and efficiency of F. variegata fruit extract, nano fruit extract, and nanogel fruit extract as attractants for S. adipata on cayenne pepper plants. The methods used to achieve these objectives were (1) LC-MS/MS analysis for phytochemical profiling, and (2) a preference test to evaluate attractant efficacy. The results revealed that the phytochemical profile of F. variegata fruit extract includes 5-hydroxy-2-(4-hydroxyphenyl)-8,8-dimethylpirano[2,3-h]chromen-4-one, with the highest compound content (68.74%) in the fruit aged 9-16 weeks. The application of nanogel extract at a 50% concentration was highly effective and efficient as a biological attractant for S. adipata on white chilli plants in Bali. These findings strongly support the development of integrated pest management strategies in Indonesia.
Eksplorasi Jamur Entomopatogen, Dari Beberapa Rizosfer Tanah, Dengan Insect Bait Method Sudiarta, I Putu; Syahbana , Raditya Darmawan; Yuliadhi, Ketut Ayu; Suputra, I Putu Wirya; Gargita, I Wayan Diksa
AGRICA Vol. 17 No. 1 (2024): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v17i1.4146

Abstract

This study aims to identify the types of entomopathogenic fungi obtained from the exploration results of the insect bait method in rhizosphere soil samples from several locations in the Bedugul area, Bali. The exploration results showed differences among the three soil samples, where the Pakcoy cultivation soil sample showed infected insect baits with Metarhizium sp. fungi. In contrast, in the Danau Buyan and Bedugul Botanical Garden soil samples, symptoms of infestation were not infected by fungi. Koch's postulate test confirmed the presence of entomopathogenic fungi in the Pakcoy cultivation soil sample, which was later identified morphologically and microscopically as Metarhizium. This study contributes to understanding the ecology of entomopathogenic fungi in the context of biological pest control, especially in sustainable agriculture.
Co-Authors ADNYANA, I MADE MEGA AGUS PUTRA SEDANA Anak Agung Ayu Agung Sri Sunari ANAK AGUNG GDE AGUNG NARENDRA ARIYANTA, I PUTU BAWA Bakar, Nor Kartini Abu CATUR KOESUMA WARDHIANY Cha, Joo Young Costa Cruz, Latizio Beni Da CRISTINE LAURINE SIMANJUNTAK Devi, Komang Saraswati Devi, Putu Shinta Devitriyani, Ida Ayu Dewa Gede Wiryangga Selangga Dinarkaya, Shah Mahapati DWI WIDANINGSIH GARGITA, I WAYAN DIKSA GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya Husni dan Zulfanazli Pudjianto Alfian Rusdy I Dewa Nyoman Nyana I G. R. M. TEMAJA I GEDE AGUS ADI WIRADARMA I Gede Ketut Susrama I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I Ketut Suada I KETUT SUMIARTHA I MADE SUDARMA I MADE SUDARMA I NYOMAN WIJAYA I Putu Sudiarta I Wayan Andi Sumartayasa I Wayan Supartha I Wayan Susila IDA BAGUS GDE PRANATAYANA IDA BAGUS GEDE MAHENDRA Klett, Katrina KORI’ ANGGRAINI Listihani, Listihani MADE SRITAMIN Mariana, Santi MERTA, I NGURAH MEGA MIFTAHUS SIROJUDDIN Mintari, Gusti Ayu Willem NI KADEK BUDARTINI NI KADEK VENIARI NI KETUT JULI DWI JAYANTI Ni Made Puspawati NI NENGAH DARMIATI NI PUTU ESA YANTI SUPARTHA NI PUTU NIA KUMARAWATI NI PUTU NIA KUMARAWATI NI WAYAN SUNITI Ni Wayan Suryati Agustini Parwata, I Made Oka A PRATIWI, NI PUTU EKA Purnomo Purnomo Putu Eka Pasmidi Ariati Putu Perdana Kusuma Wiguna SANG AYU MADE INTAN PUTRI RAHAYU Santi Mariana Selangga, Dewa Gede Wiryangga SUGIARTA, DWI Sumartayasa, I Wayan Andi SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA WAYAN ADIARTAYASA YUDHA, I KADEK WISMA