Garuda - Garba Rujukan Digital

Endang Winiati Bachtiar

Department Of Oral Biology, Faculty Of Dentistry, Indonesia University

Author-ID : 239494

Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

Published : 23 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

Matrix Metalloproteinase-9 (MMP-9) Levels in Dentinal Fluid as a Diagnostic Biomarker for Irreversible Pulpitis Megantoro, Aryo; Wairooy, Valeria Widita; Meidyawati, Ratna; Bachtiar, Endang Winiati
Journal of Dentistry Indonesia
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Objective: Pulpitis is an inflammatory condition of the dental pulp. The inflammatory status can be accurately assessed by histological examination. Biomarkers, such as matrix metalloproteinases (MMPs), are effective indicators of inflammation severity. Among these, matrix metalloproteinase-9 (MMP-9) has emerged as a key mediator of inflammatory processes associated with pulpitis. However, its clinical application as a biomarker remains limited owing to challenges in its extraction. This study aimed to evaluate the effectiveness of MMP-9 extracted from the dentinal fluid as a biomarker for pulpitis. Methods: Dentinal fluid was collected using the blunt tip of a sterile paper point, following clean excavation of the cavities. The paper point was placed in a sterile tube containing phosphate-buffered saline (PBS) and stored at −20 ºC. MMP-9 levels were analyzed using enzyme-linked immunosorbent assay (ELISA). Results: MMP-9 was detected in three of four patients diagnosed with irreversible pulpitis. Kruskal-Wallis test revealed a statistically significant difference in MMP-9 levels in the irreversible pulpitis group (p < 0.05). Conclusion: MMP-9 in dentinal fluid is a potential biomarker for irreversible pulpitis. However, further research is required to validate their diagnostic utility in clinical practice.

The Potency of miRNA 221-3p as a Molecular Biomarker Of Temporomandibular Disoder: Narrative Review Rintoko, Bimo; Tanti, Ira; Bachtiar, Endang Winiati; Bachtiar, Boy Muchlis; Prihastari, Lisa
Odonto : Dental Journal Vol 12, No 3 (2025): December 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.3.241-250

United States. The trigeminal nerve might be the source of orof facial pain. Pain can be difficult to diagnose and treat due to the variability of pain complaints. miRNA 221-3p could be a potential biomarker for pain. Genetic polymorphisms and genetic mutations can lead to changes in the activity of inflammatory mediators, sex hormones, matrix-degrading enzymes, and the immune system, which affect bone remodeling homeostasis in the temporomandibular joint.Method: Scientific databases PubMed, Scopus, and Google Scholar were searched for articles published between 2013 and 2023 in order to conduct the study. 168 carefully selected publications providing information on possible biomarkers, particularly miRNA 221-3p, were included in the review of the literatureResult: Genetic polymorphisms and genetic mutations can lead to changes in the activity of inflammatory mediators, sex hormones, matrix-degrading enzymes, and the immune system, which affect bone remodeling homeostasis in the temporomandibular joint. In addition, activity changes were also observed in neurotransmitters and pain receptors, which resulted in relatively high pain sensitivity. Thus, this condition also increases susceptibility to TMD. Genetic analysis and biomarkers of temporomandibular disorders may improve sensitivity and specificity measures in diagnosing and treating patients with TMDConclusion: As a multifactorial disease, genetic factors are risk factors for TMD. Genetic polymorphisms and genetic mutations can lead to changes in the activity of inflammatory mediators, sex hormones, matrix-degrading enzymes, and the immune system, which affect bone remodeling homeostasis in the TMD

The Potency of miRNA 221-3p as a Molecular Biomarker Of Temporomandibular Disoder: Narrative Review Rintoko, Bimo; Tanti, Ira; Bachtiar, Endang Winiati; Bachtiar, Boy Muchlis; Prihastari, Lisa
Odonto : Dental Journal Vol 12, No 3 (2025): December 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.3.241-250

United States. The trigeminal nerve might be the source of orof facial pain. Pain can be difficult to diagnose and treat due to the variability of pain complaints. miRNA 221-3p could be a potential biomarker for pain. Genetic polymorphisms and genetic mutations can lead to changes in the activity of inflammatory mediators, sex hormones, matrix-degrading enzymes, and the immune system, which affect bone remodeling homeostasis in the temporomandibular joint.Method: Scientific databases PubMed, Scopus, and Google Scholar were searched for articles published between 2013 and 2023 in order to conduct the study. 168 carefully selected publications providing information on possible biomarkers, particularly miRNA 221-3p, were included in the review of the literatureResult: Genetic polymorphisms and genetic mutations can lead to changes in the activity of inflammatory mediators, sex hormones, matrix-degrading enzymes, and the immune system, which affect bone remodeling homeostasis in the temporomandibular joint. In addition, activity changes were also observed in neurotransmitters and pain receptors, which resulted in relatively high pain sensitivity. Thus, this condition also increases susceptibility to TMD. Genetic analysis and biomarkers of temporomandibular disorders may improve sensitivity and specificity measures in diagnosing and treating patients with TMDConclusion: As a multifactorial disease, genetic factors are risk factors for TMD. Genetic polymorphisms and genetic mutations can lead to changes in the activity of inflammatory mediators, sex hormones, matrix-degrading enzymes, and the immune system, which affect bone remodeling homeostasis in the TMD

Title

Found 23 Documents
Search

Abstract

Abstract

Abstract

Title Search

Found 23 Documents Search

Abstract

Abstract

Abstract

Title

Found 23 Documents
Search