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Tingkat dan Faktor Risiko Kekebalan Protektif terhadap Rabies pada Anjing di Kota Makassar (RATE AND RISK FACTORS OF PROTECTIVE IMMUNITY AGAINST RABIES ON DOGS IN THE CITY OF MAKASSAR) Sri Utami; Bambang Sumiarto
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The rate and risk factors on protective immunity against rabies were conducted in Makassar city. Theaims of this study were to determine the level of immunity of dog and find out the association betweenmanagement factors and the level of maintenance of the dog immunity against rabies. Two hundred andforty-six serum samples were collected for measurement of dog immunity level with a method of enzymelinked immunosorbent assay (ELISA). Maintenance management data were gathered throughquestionnaires and direct observation. Analysis of data obtained by the level of immunity was donedescriptively. The significance association between the risk factors and the strength of immunity level wascalculated by using Chi square (X2) and odds ratios. The results showed only 12.2% of dogs had a protectiveantibody against rabies at > 0.5 IU/ml. Factors affecting the level of protective immunity against rabieswere the first vaccination at 1-6 months age (OR = 18.6), post-vaccination after 1-6 months (OR = 6.0), themaintenance location of dogs in urban areas (OR = 4.5), dog caring management in house (OR = 3.8), andowner income above Rp. 2.000.000/month (OR = 3.0). It is concluded that the low level of protective immunityagainst rabies and the dog bites reported each year indicates that Makassar, as endemic areal of rabies,is a threat to the outbreak of the disease in animals and humans to the surrounding areas.
Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Widodo Suwito; Widagdo Sri Nugroho; Bambang Sumiarto; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).
ISOLASI DAN IDENTIFIKASI ESCHERICHIA COLI O157:H7 PADA DAGING SAPI DI KABUPATEN BADUNG PROVINSI BALI. ISOLATION AND IDENTIFICATION ESCHERICHIA COLI O157:H7 ON BEEF AT BADUNG REGENCY PROVINCE OF BALI I Wayan Suardana; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Keamanan bahan pangan merupakan masalah yang amat penting bagi konsumen dan industri pangan. Cemaran bakteri Escherichia coli dan Coliform dianggap sebagai indikator sanitasi dalam proses pengolahan bahan pangan. Pelacakan bakteri patogen dalam pangan juga telah dilakukan secara rutin, termasuk yang bersifat zoonosis seperti Escherichia coli O157:H7. Bakteri ini menghasilkan toksin yang dikenal dengan Shiga toxin. Toksin ini dapat menimbulkan diare berdarah, colitis haemorrhagi dan hemolytic uremic syndrome (HUS) pada manusia. Dalam penelitian ini dipelajari hubungan antara tingkat cemaran dan insidensi Coliform, E.coli, E.coli O157 dan E.coli O157:H7 pada daging sapi. Bakteri pertama ditumbuhkan pada media EMBA, selanjutnya dipupuk pada media SMAC dan diakhiri dengan uji aglutinasi lateks untuk memastikan keberadaan bakteri E.coli O157 dan uji antiserum H7 untuk memastikan isolat yang diisolasi merupakan isolat E.coli O157:H7. Hasil isolasi dan identifikasi terhadap 89 sampel daging sapi diperoleh hasil rata-rata tingkat cemran coliform dan E.coli sebesar 93,01+ 2,64x103 cfu/g dan
Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; I Gusti Ngurah Kade Mahardika; Helen Scott-Orr
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The direct fluorescent antibody test (dFAT) was recommended by both World Health Organization(WHO) and Office International des Epizooties (OIE) as a standard diagnostic technique for rabies. Sincethe outbreak of rabies in Bali, it was ascertain the importance to develop a reverse transcriptase-polymerasechain reaction (RT-PCR) technique with specific primers as an alternative diagnostic method. The aim ofthis study was to develop a RT-PCR technique for rabies diagnosis in animals and find out the molecularmarker of Bali’s rabies virus (BRV) isolates based on the sequence of nucleoprotein (N) gene. Brainsamples were obtained during 2009 from 14 suspected rabid dogs and one cattle, where rabies viruseswere isolated. The dFAT was used to detect the presence of rabies viral antigen. Ribonucleic acid (RNA) ofrabies viruses was extracted with TRIzol reagent. Fragment of N gene was amplified using one-step RTPCRmethod with specifically-designed primer pairs and sequenced using ABI automatic sequencer. Multiplealignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA 4.0program. This study found that twelve out of fifteen animal brain samples confirmed as rabies by dFAT.Similarly, a single band of 1215 bp PCR product for rabies virus was also detected in twelve out of twelve(100%) dFAT rabies positive samples. It is therefore evident that alternative diagnostic of rabies inanimals can be established using RT-PCR technique. The results showed that the RT-PCR has a very highagreement with dFAT. Polymorphic sites of N gene of twelve BRV isolates were identified at the position186, 501, 801, 840, 1068 and 1153. Bali’s rabies virus isolates have conserved amino acid (isoleucine)alterations at position 308 (open reading frame). Isoleucine distinguished between all Bali’s isolates andthe all of isolates from other area of Indonesia and other part of the world. This finding significantlydifferent as compared to other rabies virus isolates from other part of Indonesia or the world documentedon the GenBank. Accordingly it is proposed that it can be used as molecular marker and believed to be thefirst study of molecular marker of rabies virus in Indonesia.
Faktor-Faktor Risiko Rabies pada Anjing di Bali (RISK FACTORS ANALYSIS FOR RABIES INDOGS IN BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; Helen Scott-Orr
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The efforts to eradicate rabies in Bali have been done for more than three years. However, therabiescases is still spreading. Thus, rabies virus continues to infect humans. A case-control study wasconducted to identify the risk factors associated with rabid dog in Bali. Cases were defined as dogsconfirmed having rabies by direct fluorescent antibody test (dFAT). Determination of sample amount ineach district was taken proportionally and samples were taken by using simple random sampling. A totalof 51 rabid dog cases between 2010 and 2011 and 102 uninfected rabies dogs as control were used in thisstudy. Possible associated factors were obtained by doing questionnaire. The data were subsequentlyanalyzed using chi-square (X2) and odds-ratio (OR) for possible association, which were ultimately analyzedby means of logistic regression to build up of model. This study revealed that factors associated with rabiddog were the status of rabies vaccination (X2= 55.538; P= 0.000; OR= 19.133; 95% CI= 8.015<OR<45.678),contact with other dog (X2= 43.659; P= 0.000; OR= 12.551; 95% CI= 5.541<OR<28.430),condition of dog(X2= 9.994; P= 0.002; OR= 3.019; 95% CI= 1.504<OR<6.058),number of raised dog (X2= 9.284; P= 0.002;OR= 2.962; 95% CI= 1.455<OR<6.027), and veterinary care (X2= 5.258; P= 0.022; OR= 2.444; 95% CI=1.125<OR<5.310). It was found an appropriate logit model to estimate probability of rabid dog events inBali province as follows : Logit Pr (rabies=1| x) = - 4.413 + 3.919 (status of rabies vaccination) + 3.457(contact with other dog). This study is expected to be used as a reference in order to improve rabies controleffectiveness in Bali.
Deteksi Produksi Toksin Stx-1 dan Stx-2 dari Escherichia coli O157:H7 Isolat Lokal Hasil Isolasi Feses dan Daging Sapi I Wayan Suardana; I Gusti Made Krisna Erawan; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Shiga toxin produced by Escherichia coli O157:H7 can cause outbreaks and sporadic cases of serioushuman diseases. The diseases are indicated by hemorrhagic colitis and hemolytic uremic syndrome. Meatand meat products have been identified as vehicles of food borne disease caused by E.coli O157:H7. Themain aim of this research was to identify the correlation between the level of E.coli O157:H7 contaminationand the presence of Shiga toxin (Stx1 and Stx2) by applying method of Vero toxin Escherichia coli-ReversePassive Agglutination Test (VTEC-RPLA). The results showed that 3 of 7 isolates and 1 of 4 isolatesisolated from feces of cattle and beef, respectively produced Stx 1 (VT1). In the detection of Stx 2 (VT2), 4of 7 isolates and 1 of 4 isolates, isolated from the same samples were found to produce this toxin.According to all isolates, in this research showed, 1 isolate was found to produce VT2, 4 isolates to produceboth VT1 and VT2, while 6 isolates showed negative results either to VT1 or VT2.
The Prevalence and Risk Factors of White Spot Syndrome Virus in Tiger Shrimp at Traditional Ponds (PREVALENSI DAN FAKTOR RISIKO WHITE SPOT SYNDROME VIRUS PADA TAMBAK UDANG WINDU TRADISIONAL) Arief Taslihan; Bambang Sumiarto; Kamiso H. Nitimulyo
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A research has been conducted to determine the status of the White Spot Syndrome Virus (WSSV)epidemic on traditional shrimp ponds in Demak regency. A cross-sectional research method was selected,by calculating samples using statistical calculation based on the assumption of 70% prevalence rate anderror (p<10%). Additionally, this research also determined the risk factors in the onset of WSSV shrimppond. Surveyed areas included Sayung and Bonang, subdistrict in Demak district, because the area areactively engaged with shrimp farming. A total of 90 traditional tiger shrimp ponds were sampled in thisstudy. The results showed that the prevalence of WSSV infected farms was 65.6% (Confident Interval,CI=55.7%-75.4%). Factors that affected high prevalence of WSSV were screening of seed (Coefficient=1.517,P<0.049), pond size (coefficient=0.0002, P<0.0089) and drying ponds (coefficient=3.756, P<0.001). Factorsthat affected the production of shrimp in ponds were analysed using multivariate regression analysis.Based on the analysis, some factors had an important role in production, i.e. : stadia of shrimp seed(coefficient=19.091, P<0.19), water depth in pond (coefficient=1.343, P<0.0024) and WSSV-infected shrimp(coefficient=-44.369, P<0.001). It was concluded that WSSV prevalence in studied area was 65.6%, and itconsidered as hyperendemic. Factors affected the WSSV prevalence were selection of seed, pond’s size anddrying ponds. Risk factors affected shrimp production in the pond were stocking seed stadia, whereasWSSV-infection of shrimp was negatively affected factor.
Prevalensi dan Faktor Resiko Kawin Berulang pada Sapi Perah pada Tingkat Peternak (PREVALENCE AND RISK FACTORS OF REPEAT BREEDING IN DAIRY COWS AT THE FARMER LEVEL) Surya Agus Prihatno; Asmarani Kusumawati; Ni Wayan Kurniani Karja; Bambang Sumiarto
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle.The cause of this syndrome may be a herd problem or a variety of individual cow problems. This studyaimed to determine the prevalence and factors which contributed in the repeat breeding syndrome in dairycattle farms in Daerah Istimewa Yogyakarta (DIY). A total of 922 dairy cows which owned by 401 farmerswere used in this study. The cows had a normal estrus cycle, have had at least once calved, age 2.5-8 yearsold, in good health condition, raised traditionally by a farmer or communal system. Multistage and clustersampling method were used in this study. The total numbers of sample at each stage was determined by proportional, whereas the total numbers of farmer was determined using “sampel rambang” Data werecollected by interviewing farmers and direct observation at the farm. Data collected were farmer’s education,length of having farm, farm condition, distance from inseminator, farmer’s ability to detect estrus andestrus cycle, the farm and cow’s hygiene. Data were analyzed by descriptive statistics then followed by ChiSquare and Odds Ratio. The results showed that the prevalence of repeat breeder at the dairy farms was29.4%. Risk factors at the herd level were the most common i.e.: estrus detection once per-day (OR = 17.8);estrus detection twice per-day (OR = 7.9); unsightly sewer (OR = 10.0); soil floor of enclosure (OR = 2.6); andthe use of wells or rivers as source of water (OR = 2.0 and OR = 1.8, respectively.
Prevalensi dan Analisis Faktor Risiko Multidrug Resistance Bakteri Escherichia coli pada Ayam Komersial di Kabupaten Blitar: Prevalence and Risk Factors Analysis of Multidrug Resistance of Escherichia coli Bacteria in Commercial Chicken, Blitar District Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i1.74

Abstract

Multidrug resistance is a problem that is difficult to overcome in terms of treating infectious diseases. Multidrug resistance is the term used to describe when a bacteria is resistant to three or more different classes of antibiotics. Escherichia coli as a commensal bacterium which has multidrug resistance, this causes more issues because Escherichia coli can transfer its resistant properties to other bacteria within the poultry digestive tract. The observational study is used to determine the risk factors and to estimate the quantitative effects arising from various components that contribute to the emergence of a disease. The sampling in this study was carried out randomly through cloaca swabs from commercial chicken farms in Blitar and 345 samples were collected. Complementary data collection was carried out using questionnaires, interviews, and field observations. The results showed the incidence of multidrug resistance in commercial chickens in the Blitar District was 72.5%. There is a relationship between causative factors with the incidence of multidrug resistance in Escherichia coli bacteria that is significantly associated with positive risk factors. The strength of this relationship can be seen from the value of OR and RR, among others factors of chicken breed (OR = 3.07; RR = 1.34), breeder's education (OR = 2.3; RR = 1.29), type of livestock business (OR = 7.5; RR = 1.43), type of animal feed (OR = 1.91; RR = 1.2), veterinary support for livestock raising management (OR = 3.09; RR = 1.44). The reference variables are whether the antibiotics are administered by non-veterinarians (OR = 2.35) or by the TS (OR = 7.92), and whether there is an antibiotic administration program (OR = 3.16; RR = 1.47). The overseeing function of farm maintenance, management, and implementation of antimicrobial administration in commercial chicken farms needs to be improved, to increase breeders' awareness of the careful usage of antibiotics and controlling the incidence of antibiotic resistance.
Analisis Sebaran Kasus African Swine Fever pada Babi Di Provinsi Sumatera Utara Tahun 2019–2020 Roza Azizah Primatika; Etih Sudarnika; Bambang Sumiarto; Chaerul Basri
Acta VETERINARIA Indonesiana Vol. 10 No. 2 (2022): Juli 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.2.164-171

Abstract

Wabah African Swine Fever (ASF) pertama di Indonesia terjadi pada tahun 2019 dan menyebar hingga tahun 2020 ke hampir seluruh kabupaten di Sumatera Utara. African Swine Fever dilaporkan menginfeksi babi domestik di peternakan. Kasus ASF dilaporkan oleh petugas veteriner melalui sistem informasi kesehatan hewan nasional Indonesia (iSIKHNAS). Tujuan penelitian ini adalah melakukan analisis kartografi untuk mengetahui sebaran arah dan pola kasus ASF tahun 2019–2020 di Provinsi Sumatera Utara. Analisis yang digunakan untuk mengetahui sebaran kasus ASF di Sumatera Utara tahun 2019-2020 adalah analisis kartografi yaitu standard deviational ellipse, sedangkan analisis pola kasus ASF menggunakan Moran’s Index. Sebaran kasus ASF tahun 2019 mengikuti arah barat laut-tenggara, sedangkan pada tahun 2020 mengikutiarah timur-barat. Pola kejadian kasus ASF tahun 2019 dibagi menjadi 4 kuadran, terdiri dari 34 kasus di kuadran pertama, tinggi tinggi (HH) dan 203 kasus di kuadran ketiga, rendah rendah (LL). Terdapat satu kasus di area hotspot dan 36 kasus di area coldspot. Pola kejadian kasus ASF tahun 2020 terdiri dari kuadran I tinggi tinggi (HH) sebanyak 81 kasus dan kuadran III rendah rendah (LL) sebanyak 173 kasus. Terdapat 10 kasus di area hotspot dan 55 kasus di area coldspot. Upaya pengendalian ASF dapat dilakukan di wilayah hotspot dan upaya pencegahan ASF dapat dilakukan di wilayah coldspot.