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Journal : BERITA BIOLOGI

KERAGAMAN GENETIK, HERTTABILITAS DAN KOEFEIEN VARIASI GENETIK BEBERAPA KARAKTER GALUR MUTAN KEDELAI (Glycine max (L.) MOT.) Ishak Ishak; Soertini Gandanegara
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1262

Abstract

Analysis of genetic variance, heritability and coefficient of genetic variance were carried out for seven characters of soybean mutant lines in M3 generation, which were conducted at CAIR-Field Station, Pasar Jumat, Jakarta.Results showed that the highest of heritability value about 85.50% was obtained for seed weight, and followed by number of pods,biomass of stem and shelpedpods, root/stem ratio, seed weight/ Zpods ratio, and the lowest heritability value was biomass of root around 26,83% Analysis of coefficient of genetic variance from seven characters of soybean mutants indicated value ranging from 10.00-39,50%.From the result of experiment can be concluded that gamma irradiation produced high genetic variability in M3 generation and contributed positively for crops improvement through breeding programme
INDUKSI KALUS DAN REGENERASI TANAMAN DARI KULTUR ANTERA PADI Fl HYBRID (ORYZA SATIVA L.) Ita Dwimahyani; Ishak Ishak; Sobrizal Sobrizal
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1094

Abstract

A haploid breeding program was initiated to develop doubled haploid blast tolerance rice breeding via anther culture.Blast tolerant variety (Laka) was crossed with Kencana Bali (sensitive to blast).Anthers from F1 were cultured on two kind of media (combination of N6 macro salt and MS micro salt) containing 1 mg/l NAA + 0,1 mg/l kinetin called medium-1 and another one containing 1 mg/l 2,4-D + 0,1 mg/l kinetin called medium-2.Anthers were treated with cold shock (4 °C)for 5 and 10 days before cultured.Results of experiment showed that F1 plants derived anthers were able to form call Number ofplantlets were produced during anthers culture consisted of 482 green plants and 50 albinos. Most of green plants did not produced root.Root growth of plantlets were induced with 1.5-4.5 mg/l IBA in MS medium.
IDENTIFIKASI KERAGAMAN GENETIK ANTARA PELITA I/I DAN ROJOLELE MENGGUNAKAN MARKAH RAPD Ishak Ishak
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1095

Abstract

The study of genomic diversity in molecular level on two sub-species i.e indica andjavanica rice types were represented by cv.Pelita 1/1 and Rojolele respectively.Javanica called padi bulu due to long aunt on the grain. Polymerase Chain Reaction (PCR)with 17 primers have been used for detecting genomic variation between these two sub-species.Results of experiment indicated that 12 out of 17 primers showed polymorphic DNA.This study would assist plant breeder to decide,whether to improve rice quality through crossing between javanica and indica is necessary.