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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Primatologi Indonesia Jurnal Sain Veteriner Jurnal Veteriner Microbiology Indonesia Seminar Nasional Teknik Kimia Kejuangan Majalah Obat Tradisional Jurnal Pendidikan Usia Dini ZOO INDONESIA The Indonesian Biomedical Journal VISI : Jurnal Ilmiah Pendidik dan Tenaga Kependidikan Pendidikan Non Formal CARADDE: Jurnal Pengabdian Kepada Masyarakat Jurnal Respirologi Indonesia Indonesian Values and Character Education Journal Zoo Indonesia Jurnal Kedokteran Hewan Jurnal Pendidikan dan Pengabdian Masyarakat The International Journal of Tropical Veterinary and Biomedical Research International Journal of Educational and Psychological Sciences (IJEPS) International Journal of Integrated Science and Technology Jurnal Pendidikan Anak Berita Biologi

Pamungkas, Joko

Fakultas Kedokteran Hewan, Institut Pertanian Bogor

Author-ID : 2420535

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Kajian Equine Infectious Anemia pada Kuda Impor di Bandar Udara Internasional Soekarno-Hatta Marlefzena Marlefzena; Sri Murtini; Joko Pamungkas
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Equine Infectious Anemia (EIA) disease is caused by EIA Virus (EIAV) from genus Lentivirus, subfamily Orthoretrovirinae, family retroviridae. This virus causes a persistent infection and potentially fatal in Equidae. There is no scientific literature yet for this disease in Indonesia. In order to determine this disease in our country, it is necessary continuing laboratory detection in imported Equidae and conducted further surveillance in Equidae throughout Indonesia. This research is aimed (1) to detect of any antibodi titre of EIAV in imported horse, (2) To get information about the relationship between laboratory result with the secondary datas of origin country. The EIAV antibody detected by indirect enzyme-linked immunosorbent assay (indirect ELISA). The secondary datas were collected from Health Certificate of origin country, World Animal Health Information System (WAHIS) Interface OIE and literatures. Total of 133 blood samples were collected from imported horse, ex-imported horse and lokal breed horse. The result showed that all of imported horse, ex-imported horse and lokal breed horse were negative antibody against EIAV. The initial information on Equine Infectious Anemia indicated that health requirement in imported horse has fulfilled, so that imported horse was free from EIAV.

SENYAWA BIOAKTIF TORBANGUN (Plectranthus amboinicus (Lour.) SPRENG) FRAKSI KLOROFORM INDUKSI APOPTOSIS PADA KANKER PAYUDARA (SEL MCF-7) IN VITRO Wahid Yulianto; Nuri Andarwulan; Puspo Edi Giriwono; Joko Pamungkas
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Torbangun (Plectranthus amboinicus (Lour.) Spreng) is a medicinal plant that has been traditionally used in tropical countries to cure various illnesses. The objective of this study was to identify the active compounds in the chloroform fraction which have effect on the apoptosis-related genes expression of breast cancer MCF-7 cells. Apoptosis was observed morphologically using Hoechst nuclear staining. Expression of the genes was analyzed using Real-Time PCR. Chemical compounds of the plant fractions were determined using LC-MS. Result of cell morphology observation clearly indicated apoptosis after the treatment of the plant fraction. Increased expression of anti-apoptotic gene Bcl-2 could not prevent the cells from apoptosis. Expressions of p53 and p21 genes were increased significantly. The expressions of caspase 9, caspase 7 and caspase 1 were increased at concentration-dependent manner. Most of the compounds in the chloroform fraction are identified as diterpenoids which may contribute to the apoptosis inducing activity of the fraction.

Expression of Simian Retrovirus Type D Serotype 2 Envelope in Insect Cell Using Baculovirus Expression Vector System DIAH ISKANDRIATI; MOHAMAD SADIKIN; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Simian retrovirus type-D (SRV) is a causative agent of simian acquired immunodeficiency syndrome in Asian macaques, and can serve as a viral model in understanding of retrovirus infection because of some similarities to human AIDS pathogenesis. Study of infection and pathogenesis of SRV in macaques could be a strategy of vaccine and antiviral development for preventive and therapeutic purposes. We expressed the SRV-2 envelope gene using baculovirus expression vector system and transfected it to Spodoptera frugiferda insect cell line for SRV-2 recombinant protein production. Analysis using PCR and sequencing technique of recombinant in the passage-3 viral stock indicated the occurrence of recombination between SRV-2 envelope and baculovirus genome. Purification using immobilized metal ion affinity chromatography Ni2+-NTA to recombinant protein could minimize the presence non-specific proteins. The SDS-PAGE analysis showed a specific protein for SRV-2 gp70 envelope. Western blot analysis of this purified protein indicated a specific reaction with anti-SRV-2 antibody positive of Macaca fascicularis serum shown as SRV-2 gp70 envelope band.

Macaca nemestrina and Dengue Virus Infectivity: a Potential Model for Evaluating Dengue Vaccine Candidates SUSANA WIDJAJA; IMELDA WINOTO; JONATHAN STURGIS; CHAIRIN N MAROEF; ERLIN LISTIYANINGSIH; RATNA TAN; JOKO PAMUNGKAS; PATRICK J BLAIR; DONDIN SAJUTHI; KEVIN RANDALL PORTER
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Macaca nemestrina has been shown to respond to infectious disease agents, such as HIV, and is more sensitive compared to other species of macaques such as rhesus (M. mulatta) and cynomolgus monkeys (M. fascicularis). To evaluate M. nemestrina for the ability to support dengue (DEN) viremia and serve potentially as an improved model for testing DEN vaccines, a series of experiments were conducted using primary viral isolates from individuals with DEN virus infections. This study shows that M. nemestrina develops consistent, measurable viremia with all four DEN serotypes and produces immune responses sufficient to protect against homologous virus. Anti-dengue antibodies generated after infection are predominately IgG1. This species of monkey therefore appears to be a suitable model for testing DEN virus vaccine candidates.

Isolation and Characterization of Simian Retrovirus Type D from Macaca fascicularis and M. nemestrina in Indonesia DIAH ISKANDRIATI; UUS SAEPULOH; SILMI MARIYA; RICHARD F GRANT; DEDY DURYADI SOLIHIN; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (AIDS) in Asian macaques. In the past, SRV isolates from macaques had only been identified at the US primate centers, outside the country of origin and after the animals had been introduced into a new environment. In this study, we report the first isolation, cultivation and molecular characterization of the type D simian retrovirus naturally infecting wild caught macaques in their natural habitats in the country of origin, in this case, Indonesia. When peripheral blood mononuclear cells (PBMC) from Macaca fascicularis (Mf) and M. nemestrina (Mn) were co-cultured with Raji human B-cell line, syncytia were observed microscopically and confirmed by immunofluoresence assay using antibody to SRV-2. Immunoblot analysis of purified Mf-ET1006 from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV. Sequence analysis of Mf isolates in the viral envelope region revealed high homology to SRV-2 (94-96%). On the other hand, the homologies in the envelope region of Mn isolates were less than 80% to SRV-1, SRV-2, SRV-3 and Mf isolates. This study suggests that the isolate from Mn may be different from any other published SRV isolates.

Dissemination in Pigtailed Macaques after Primary Infection of Dengue-3 Virus JOKO PAMUNGKAS; DIAH ISKANDRIATI; UUS SAEPULOH; MOSES AFFANDI; ESTHER ARIFIN; YASMINA PARAMASTRI; FITRIYA NUR ANISA DEWI; DONDIN SAJUTHI
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Nonhuman primates (NHPs) play as indispensable animal model in biomedical research for studying a variety of human health issues, diseases and disorders, therapies, and preventive strategies. Since the immunological and physiological responses of NHPs, at some extent, to experimental viral infections are similar to humans, it is possible that studies of dengue infection in NHPs may aid understanding of dengue infection in humans. In this study,we used pigtailed macaques (Macaca nemestrina) as the experimental animal to study dengue-3 (DEN-3) virus infection.We evaluated DEN-3 viral distribution and replication sites after a primary infection in all collected tissues. Sequential localization in tissue of DEN-3 virus was studied in pigtailed macaques euthanized three days post viral inoculation (10 pfu mL ). Pigtailed macaque that was inoculated subcutaneously or intravenously; showed the highest viremia (62.94 pfu mL and 58.62 pfu mL ) detected by one step reverse transcription real time PCR. The virus inoculated in pigtailed macaques by subcutaneous injection was rapidly disseminated from the inoculation site to the lymph nodes, adrenal glands, kidneys, heart, thyroid, liver, prostate gland, and seminal vesicles. Meanwhile, dissemination of dengue virus in pigtailed macaques inoculated intravenously was detected in lymph nodes, thymus, salivary glands, liver, and prostate gland. This study suggested that the above mentioned-tissue specimens are involved or affected by DEN-3 virus replication and the route of infection seemed to have influenced the virus dissemination.

Cloning and Expression of Serotype-2 Simian Betaretrovirus Reverse Transcriptase Gene Isolated from Indonesian Cynomolgus Monkey in Escherichia coli UUS SAEPULOH; DIAH ISKANDRIATI; FUNGKEY HOETAMA; SELA SEPTIMA MARIYA; DEDY DURYADI SOLIHIN; JOKO PAMUNGKAS; DONDIN SAJUTHI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

In this study, we isolated the simian betaretrovirus serotype-2 (SRV-2) reverse transcriptase (RT) gene from infected Indonesian cynomolgus monkey (Macaca fascicularis). The gene was then cloned in Escherichia coli expression system. The SRV-2 RT gene is located between nucleotides 3284-4925 in the polyprotein (Pol) region encodes 547 amino acids. Analysis of expression using SDS-PAGE and western blot techniques showed a specific band of 64.9 kDa, indicating SRV-2 RT recombinant enzyme. Purification of SRV-2 RT recombinant enzyme produced 312 μg mL-1 protein with 7.1 U μL-1 enzyme activities. Application of this recombinant enzyme in reverse transcription-PCR (RT-PCR) of β-globin and β-actin genes produced DNA fragments of 206 and 350 bp, indicating amplification of β-globin and β-actin genes, respectively. Therefore, the expressed SRV-2 RT enzyme was proven to be functional, although the activity was low.

POLA AKTIVITAS DAN STRATIFIKASI VERTIKAL OLEH MONYET EKOR PANJANG (Macaca fascicularis RAFFLES, 1821) DI FASILITAS PENANGKARAN SEMI ALAMI PULAU TINJIL, PROPINSI BANTEN Purbatrapsila Azhari; Entang Iskandar; Joko Pamungkas
ZOO INDONESIA Vol 21, No 1 (2012): Juli 2012
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v21i1.2349

Monyet ekor panjang merupakan jenis primata yang memiliki kemampuan adaptasi yang tinggi untuk bertahan hidup pada berbagai tipe habitat yang berbeda. Kemampuan ini berkaitan dengan bagaimana jenis ini memanfaatkan sumberdaya yang terbatas di habitatnya selama waktu aktif untuk memenuhi kebutuhan hidupnya di alam. Tujuan penelitian ini adalah untuk menentukan pola aktivitas dan penggunaan strata vertikal oleh kelompok monyet ekor panjang yang sudah terhabituasi dengan baik di fasilitas penangkaran semi alami Pulau Tinjil, Propinsi Banten, Indonesia. Kelompok monyet ekor panjang diamati menggunakan metode Scan Sampling dengan interval lima menit antara bulan Maret dan September 2011. Berdasarkan hasil penelitian ini, kelompok monyet ekor panjang menggunakan waktu aktifnya paling banyak untuk berpindah (36,01%) dan beraktivitas paling banyak pada ketinggian 1-5 meter diatas tanah (31,98%).

Association of CCL7 Promoter Polymorphism with Responsiveness to Allergen in Cynomolgus Macaque Model of Asthma Sela Septima Mariya; Fitriya Nur Annisa Dewi; Eric Hayes; Villiandra Villiandra; Yasmina Arditi Pramastri; Diah Iskandriati; Uus Saepuloh; Joko Pamungkas; I Nengah Budiarsa; Dondin Sajuthi
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.1093

BACKGROUND: C-C motif Ligand 7 (CCL7) has been reported to be associated with asthma severity in humans. Cynomolgus macaques (Macaca fascicularis; Mf) are often used as animal model of asthma but little is known about Mf genetic profile such as polymorphism. Our aim was to identify CCL7 polymorphism in Mf as a potential surrogate marker for identification of allergen responsiveness in the Mf model of asthma.METHODS: Real-time PCR was performed on archive of bronchoalveolar fluid samples previously collected from Mf that were exposed to allergen. Expression of CCL7 mRNA was evaluated, and sequencing technique was used to identify polymorphism in this gene.RESULTS: The results showed that CCL7 expression did not differ between Mf, despite a trend of lower expression in Mf that exhibited high response to allergen. By direct DNA sequencing of CCL7, 10 sequence variants were identified; three in promoter region (-460 G/A, -459 A/G, -456 -/A ), two in exon 1 (9 A/G, 65 G/C), four in intron 1 (135 T/C, 254 T/C, 420 T/C, 453 A/G), and one in intron 2 (1205 T/A).CONCLUSION: There was an association between Mf sensitivity to allergen with CCL7 promoter polymorphism at (-456 -/A). These results suggest that CCL7 may be a potential genetic marker to identify Mf sensitivity to allergen, which could be a useful tool to efficiently select for Mf model of asthma.KEYWORDS: asthma, CCL7, allergy, Ascaris suum, nonhuman primate

CCL2 and CCR2 Expression in Broncoalveolar Lavage Fluid of Cynomolgus Macaque Model Of Asthma Sela S Mariya; Fitriya N Dewi; Villiandra Villiandra; Yasmina A Pramastri; Diah Iskandriati; Eric Hayes; Joko Pamungkas; R.P Agus Lelana; Ligaya I Tumbelaka; Dondin Sajuthi
Jurnal Respirologi Indonesia Vol 38, No 2 (2018)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v38i2.166

Background: Animal models are essential for the development and improvement of novel and effective methods for diagnostic and treatment of human diseases. Cynomolgus monkeys have been used as animal model in asthma studies wherein they exhibited different responses to allergen exposure in the airway; some were low responders while others were high responder. CCL2 is a potent chemotactic factor for monocytes and the gene expression was high in animal model of asthma. The aim of this study was to evaluate CCL2 and CCR2 expression between the low and high responders. Methods: Realtime PCR technique was used to evaluate CCL2 and CCR2 gene expression in bronchoalveolar lavage samples. The subject of this study was asthmatic cynomolgus monkeys, consisted of 4 low responders and 4 high responders. This study was held in Biotechnology Laboratory of Primate Research Center LPPM IPB on October 2016- January 2017. Results: CCL2 and CCR2 expression in low responders were higher than those in high responders at 24hours post airway challenge. Conclusion: CCL2 may potentially be developed as target for therapy or a genetic marker for asthma responsiveness in individuals. (J Respir Indo 2018; 38(2): 115-22)

Co-Authors - Sudarmanto Adi Dieni Maulana Rizka Agustiningsih, Rini Alexander Nater Amir Syamsudin Andi PM Yusmanto Ann Schmidt Aris Tri Wahyudi Azhari Purbatrapsila Bambang Bintarto Bambang Joeniman Budiman Bela CHAIRIN N MAROEF DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati DIAH ISKANDRIATI Diah Iskandriati DIAH ISKANDRIATI Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Eka Sapti Cahyaningrum Ellis Dwi Ayuningsih Ellis Dwi Ayuningsih Entang Iskandar Eric Hayes Eric Hayes ERLIN LISTIYANINGSIH ESTHER ARIFIN Faisal Faisal Farah S Normaysti Fatia Rosyida, Fatia Febrita Cipta Putranti FUNGKEY HOETAMA Gede Eko Darmono Gusti Zakaria Anshari I Nengah Budiarsa I NENGAH BUDIARSA I wayan Teguh Wibawan Ika Budi Maryatun IMELDA WINOTO Indyah Wahyuni Jansen Manansang JONATHAN STURGIS Julaika, Weni Karissa, Violeta Chandra KEVIN RANDALL PORTER LaRene Kuller Lila . Lilik Prayitno Margaret E Thouless Marlefzena Marlefzena Martha Christianti MARYATI SURYA Michael Krützen Mohamad Sadikin MOSES AFFANDI Muthmainah Nangoy, Meis Nelva Rolina Ni Luh Putu Ika Mayasari Novita Anggraeni Nur Hayati Nuri Andarwulan PATRICK J BLAIR Permana wati PERMANAWATI PERMANAWATI Puji Rianti Purbatrapsila Azhari Puspo Edi Giriwono R. Suryo Saputra R.P Agus Lelana Rachmitasari Noviana RATNA TAN RICHARD F GRANT Robin Watanabe Safriyanto Dako Sela S Mariya Sela S. Mariya SELA SEPTIMA MARIYA Sela Septima Mariya Septiantina Dyah Riendriasari Septika Cahya Rahmawati Silmi Mariya SILMI MARIYA Silvia Triwidyaningtyas Sofia Hartati sri murtini . Sriyanto Sriyanto Sudaryanti SUSANA WIDJAJA TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tiltje Andretha Ransaleh Uus Saepuloh UUS SAEPULOH Uus Saepuloh Uus Saepuloh Uus Saepuloh UUS SAEPULOH Villiandra Villiandra Villiandra Villiandra Villiandra Villiandra VINCENTIUS ARCA TESTAMENTI Wahid Yulianto Walberto Sinaga Wenty Dwi Febriani William R Morton Wirdateti . Y. Fitrianis Y. Paramastri Yasmina A Pramastri Yasmina A. Paramastri Yasmina Arditi Pramastri YASMINA PARAMASTRI Yunetta Putri Arios

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