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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Zuriat Microbiology Indonesia Jurnal Agroekoteknologi JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrosains: Jurnal Penelitian Agronomi Jurnal Pertanian Agros JURNAL AGROHITA Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Hilirisasi IPTEKS (JHI) Agroteknika Agro Bali: Agricultural Journal Akta Agrosia Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jagur: Jurnal Agroteknologi
Aprizal Zainal
Andalas University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Agrosains: Jurnal Penelitian Agronomi

 1 
Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro Amalina Wahyuni; Benni Satria; Aprizal Zainal
Agrosains : Jurnal Penelitian Agronomi Vol 22, No 1 (2020): Agrosains : Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.65 KB) | DOI: 10.20961/agsjpa.v22i1.36007

Abstract

Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact.  Yellow callus and compact structure could be utilized for organogenesis.
The Effect of Benzyl Amino Purine (BAP) on Indirect Organogenesis of the Titan Arum (Amorphophallus titanum (Becc.)) Putri Salsa Afifah; Yusniwati Yusniwati; Aprizal Zainal; Ryan Budi Setiawan
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 2 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i2.95804

Abstract

The titan arum (Amorphophallus titanum (Becc.)), an endangered and endemic flora from the island of Sumatra, faces a significant risk of extinction. Conservation efforts for the titan arum were undertaken, including in vitro conservation through tissue culture. The study aimed to determine the optimal concentration of the growth regulator Benzyl Amino Purine (BAP) for inducing shoot formation in titan arum and to observe cellular changes during the shoot stage through histological test. This research was conducted from November 2023 to March 2024 at the Tissue Culture Laboratory, Andalas University. The experiment was arranged in a Completely Randomized Design (CRD) with five treatment levels: 2, 3, 4, 5, and 6 ppm.  Data analysis was performed using an F-test at a 5% significance level. If the F-test showed significant differences, further testing was carried out using Duncan's Multiple Range Test (DMRT) at a 5% significance level. Meanwhile, data on explant rooting percentage and root count were presented as means and standard deviations. Results indicated that various concentrations of BAP could induce shoots formation of Amorphophallus titanum Becc., with the percentage of explants forming shoots reaching 80–95%.  The concentration of 5.0 ppm BAP was the most effective, yielding the highest average number of shoots at 7.80. Histological test revealed cell enlargement at the shoot tips. This research is pivotal for the conservation of titan arum and promotes further studies in in vitro culture techniques.
Co-Authors . Giyanto Afrima Sari Akhir, Nasrez Amalina Wahyuni Anwar, Aswaldi Aprissilia Taifani Galuh Utomo Arnelio, Rangga Atzim Baitur Rahman Auzar Syarif Bahagiawati Amirhusin Benni Satria Daulay, Azri Gilang Ramadhan ekawati, fitri Etti Swasti Giyanto . Gustian Gustian Haliatur Rahma, Haliatur Hasbullah, Farhan Hasmiandy Hamid Hervani, Dini Indra Dwipa Irfan Suliansyah Mawati, Idel Meity S Sinaga, Meity S Memen Suharman, Memen Mufti Gustrianda Musliar Kasim Nasution, Rizki Nia Sukri Netti Herawati Nissa, Ayu Khairun Nugraha Ramadhan P.K. Dewi Hayati P.K. Dewi Hayati Pagil Prawinata Panca Jarot Santoso Putri Salsa Afifah Putri, Yenni Anisah Ramadhan, Nugraha Rini Suryani Rini Suryani, Rini Roza Yunita Ryan Budi Setiawan Sari Rahmadhini Satriyas Ilyas SATRIYAS ILYAS Silvia Permata S Sudarsono Sukartini Sukartini Sukartini Suryati . Sutoyo Sutoyo Sutoyo Sutoyo Teguh Budi Prasetyo Trisia Wulantika UJANG KHAIRUL Warnita Winda Purnama Sari Yusniwati Yusniwati