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All Journal Buletin Agrohorti Jurnal Fitopatologi Indonesia Bioplante Microbiology Indonesia Jurnal Keteknikan Pertanian AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian

Widodo Widodo

Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Kamper Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 2432568

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology

Published : 29 Documents Claim Missing Document

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EKSPLORASI CENDAWAN ENDOFIT DARI TANAMAN CABAI YANG BERPOTENSI SEBAGAI AGENS BIOKONTROL PENYAKIT ANTRAKNOSA (Colletotrichum acutatum L.) (Exploration of Endophytic Fungi from Pepper as Biological Control Agents of Anthracnose (Colletotrichum acutatum L.) Weni Wilia; . Widodo; Suryo Wiyono
Bioplantae Vol. 2 No. 1 (2013)
Publisher : Bioplantae

Show Abstract | Download Original | Original Source | Check in Google Scholar

Anthracnose caused by Colletotrichum acutatum is one of devastated disease of pepper in Indonesia and has great impact on yield loss. Most of farmers use fungicides as controller for this disease. Application of biocontrol agents could be solution to control anthracnose. Beneficial microorganism, such as endophytic fungi are able to be promising biocontrol agents of some pathogens, including Colletotrichum acutatum. The aim of this research was to isolate endophytic fungi from fruit and branch as biocontrol agents for pepper anthracnose. The research resulted 11 isolates of endophytic fungi that were further tested against anthracnose. Isolates of endophytic fungi were identified as steril hifa hitam 1, steril hifa hitam 2, steril hifa 1, steril hifa 2, Nigrospora sp., Coniotyrium sp., Cylindrocarpon sp., Paecilomyces sp. The 3 of other isolates (isolat buah 5, isolat buah 1dan isolat batang 24) were not identified yet. Keyword : Pepper, antrachnose, C. acutatum, endophytic fungi

Fusarium Species Associated with Corm Rot of Taro in Bogor . WIDODO; . SUPRAMANA
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

In Bogor-West Java, corm rot disease of taro has devastated in many cropping areas and caused yield losses up to 70%. During preliminary studies, Fusarium species were constantly recovered when diseased materials, rotten corms and discolored petioles were incubated. The objectives of this study was to identify the Fusarium species associated with the corm rot disease in Bogor and determine their pathogenicity and hosts range. Samples were collected from 40 diseased corm samples taken in 9 sub-districts of Bogor. Two species, viz, Fusarium solani and F. oxysporum were identified based on the morphological characteristics. Among 40 Fusarium isolates recovered in this study, 70% were Fusarium solani and 30% were F. oxysporum. F. solani could infect to all tested edible Araceae , while F. oxysporum was only pathogenic to Colocasia esculenta. Both species of Fusarium did not cause any symptoms when inoculated on selected ornamental and legume crops. These results gave the indication that F. solani was probably pathogenic only to edible Araceae, but further inoculation assay on living taro plants are necessary to carry out in order to clarify this result.

Selection of Yeasts Antagonists as Biocontrol Agent of Mango Fruit Rot caused by Botryodiplodia theobromae DWI SUGIPRIHATINI; SURYO WIYONO; . WIDODO
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Fruit rot caused by Botryodiplodia theobromae is one of the most important post harvest disease of mango in Indonesia. Study on biological control on the disease is required to develop environmentally-sound control technology. The research objectives were to study the potency of yeasts in controlling post harvest mango disease i.e. fruit rot caused by B. theobromae and mechanism involve in the biocontrol. Total yeast isolates used for screening were twenty one, four from collection of Plant Clinic of Institut Pertanian Bogor, and twenty one isolated from healthy mango skin. All of yeast isolates were characterized and identified using BIOLOG. Bioassay on antagonistic activity of yeasts against fruit rot, in-vitro dual culture test and chitinolytic activity were carried out. Cryptococcus albidus var. aerius WSW1, Pichia guilliermondii K1, and Debaryomyces hansenii K12 were the three most effective antagonistic yeasts against B. theobromae with effectiveness of 70.83%, 45.83%, 37.50% respectively. In vitro bio-assay showed that C. albidus var. aerius WSW1, C. albidus WSW2, C. albidus K6, C. terreus YSW1, Candida edax OSW1, Candida edax K13, and Cryptococcus luteolus K2 had high antibiosis activity. Biocontrol activity of tested yeasts against fruit rot of mango did not correlate to its antibiosis and chitinolytic activity

Penggunaan Vis-NIR untuk Deteksi Serangan Huanglongbing pada Daun Jeruk Raden Arief Firmansyah; Kudang Boro Seminar; Widodo .
Jurnal Keteknikan Pertanian Vol. 5 No. 1 (2017): JURNAL KETEKNIKAN PERTANIAN
Publisher : PERTETA

AbstractHuanglongbing is citrus disease which is a major threat for citrus orchard. Neither disease has a cure nor an efficient means of control. Early detection is important to prevent development and spread of the disease. The most effective detection used DNA test by PCR. However, identification used DNA test required sample preparation, time-consuming and expensive. The objective of this study was to build detection of healthy and HLB-infected leaves software. The leaf samples collected from citrus orchard in Situgede village, Bogor. Sampleleaves divided into three group, Huanglongbing-infected leaves, healthy leaves and asymptomatic leaves. All samples was tested by PCR for verification visual symptoms of huanglongbing. Vis-NIR spectrometer with a spectra range of 339 to 1022nm was used to acquisition HLB-infected and healthy leaves spectral data. MSC, SNV, baseline correction, first and second derivative were used for pretreatment method. Artificial neural network was used to build classification model. X-loading plot from principal component analysis was used to obtain sensitive wavelength. Classification for healthy and HLB-infected classs used sensitive wavelength baseline correction-based had the best performance and high accuracy (100%). The classification model was embedded in software PC-desktop based which was used visual basic programming language. Asymptomatic leaves spectral from HLB-positive tree were used to testing classification model. Model classified data into HLB-infected group, which was consistent with PCR test. The result from this study indicated that developed software could be used to HLB detection in early stage of disease.AbstrakHuanglongbing adalah penyakit jeruk yang merupakan ancaman utama bagi budidaya jeruk. Tidak ada pengendalian yang tepat untuk Huanglongbing. Deteksi dini penting untuk mencegah penyebaran dan pengembangan penyakit ini. Deteksi dini yang paling efektif menggunakan tes DNA dengan PCR. Namun, identifikasi menggunakan tes DNA memerlukan persiapan sampel, memakan waktu dan mahal. Tujuan daripenelitian ini adalah membangun perangkat lunak deteksi daun sehat dan terinfeksi HLB. Sampel daun dikumpulkan dari kebun jeruk di Desa Situ Gede, Bogor. Sampel daun dibagi menjadi tiga kelompok, daun yang terinfeksi HLB, daun sehat dan daun belum bergejala. Semua sampel telah diuji dengan PCR untuk verifikasi gejala visual Huanglongbing. Spektrometer Vis-NIR dengan rentang spektrum dari 339-1022nm digunakanuntuk mengumpulkan data spektrum daun terinfeksi HLB dan sehat. MSC, SVN, baseline correction, turunan pertama dan kedua dari spektra digunakan sebagai metode praperlakuan. Jaringan syaraf tiruan digunakan untuk membangun model klasifikasi Plot X-loading dari analisis komponen utama digunakan untuk mendapatkan panjang gelombang sensitif. Klasifikasi terhadap kategori daun sehat dan sakit menggunakan panjang gelombang sensitif berbasis baseline correction memiliki nilai akurasi 100 % dan kinerja terbaik. Model klasifikasi yang ditanam pada perangkat lunak berbasis komputer desktop menggunakan bahasa pemrograman visualbasic. Data spektrum daun belum bergejala dari pohon positif terinfeksi HLB digunakan untuk menguji model klasifikasi. Model mengklasifikasikan data tersebut ke kelompok terinfeksi HLB, yang konsinten dengan hasil pengujian PCR yang juga mengelompokkan pada daun terinfeksi HLB. Hasil penelitian ini menunjukkan bahwa perangkat lunak dapat digunakan untuk deteksi HLB pada tahap awal perkembangan penyakit.

IDENTIFIKASI KETAHANAN PLASMA NUTFAH KARET IRRDB 1981 TERPILIH TERHADAP PENYAKIT GUGUR DAUN CORYNESPORA BERDASARKAN AKTIVITAS TOKSIN CASSICOLIN Fetrina Oktavia; Sudarsono Sudarsono; Kuswanhadi Kuswanhadi; Dini Dinarty; Widodo Widodo
Jurnal Penelitian Karet JPK : Volume 34, Nomor 1, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i1.225

Salah satu penyakit pada tanaman karet adalah penyakit gugur daun Corynespora (PGDC) yang disebabkan oleh jamur Corynespora cassiicola. Patogen tersebut dapat menyerang semua tahap pertumbuhan tanaman karet yang menyebabkan terjadinya penurunan hasil lateks yang cukup signifikan dan bahkan dapat menyebabkan kematian tanaman. Penggunaan klon-klon tahan sebagai bahan tanam merupakan cara yang paling efektif dan ekonomis untuk mencegah terjadinya serangan PGDC. Karena itu identifikasi klon-klon resisten merupakan strategi utama dalam manajemen PGDC. Penelitian ini bertujuan untuk mengidentifikasi tingkat ketahanan 50 genotipe terpilih plasma nutfah IRRDB 1981 (PN’81) terhadap PGDC. Enam klon karet yang berasal dari populasi Wickham digunakan sebagai pembanding (BPM 24, BPM 1, GT 1, RRIC 600, PB 260 dan RRIM 600). Empat isolat C. cassiicola (CC-01, CC-20, CC-22, dan CC-23) diinokulasikan masing-masing pada daun muda tahap pertumbuhan B2C, dan intensitas kelayuan daun dihitung berdasarkan estimasi kehilangan air akibat aktifitas patogenisitas toksin. Hasil penelitian menunjukkan bahwa 12 genotipe tergolong sangat tahan, 13 genotipe tergolong tahan, 23 genotipe tergolong rentan dan 8 genotipe tergolong sangat rentan. Genotipe PN 451, PN 494 dan PN 604 menunjukkan tingkat ketahanan yang lebih baik terhadap PGDC dibandingkan dengan populasi Wickham sehingga ketiga genotipe tersebut berpotensi digunakan sebagai sumber gen ketahanan dalam program pemuliaan tanaman karet. Diterima : 13 Maret 2016 / Direvisi : 29 Juli 2016 / Disetujui : 2 Agustus 2016 How to Cite : Oktavia, F., Sudarsono, S., Kuswanhadi, K., Dinarty, D., & Widodo, W. (2016). Identifikasi ketahanan plasma nutfah karet IRRDB 1981 terpilih terhadap penyakit gugur daun Corynespora berdasarkan aktivitas toksin Cassicolin. Jurnal Penelitian Karet, 34(1), 35-48. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/225

Potensi Cendawan Asal Tanah Perakaran Bambu Sebagai Endofit dan Agen Biokontrol Penyakit Akar Gada Pada Tanaman Brokoli . Asniah; . Widodo; Suryo Wiyono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The potential of fungi from bamboo rhizosphic soils as endophytic and biocontrol agents of clubroot disease (Plasmodiophora brassicae) on Brocoli. Clubroot, caused by Plasmodiophora brassicae is a serious soilborne disease of plants worldwide, capable of severe infection on broccoli, cabbage and cauliflower. Clubroot is the most destructive diseases on cruciferae in Indonesia. The existing control measures, including biological control do not provide satisfactory result. The objective of the study was to explore fungi from bamboo rhizospheric soils as endophytic that can suppress clubroot disease in broccoli. There were two steps in this study: (1) exploration and identification of fungi from bamboo rhizospheric soils. (2) Effectiveness test of the fungi in suppressing clubroot disease. There were four species of fungi as endophytes in brocoli in this study, e.g Aspergillus sp., Mortierella sp., Paecilomyces sp., and Chaetomium globosum. They had colonized broccoli root endophytically and suppressing clubroot disease. Paecilomyces sp. of endophytic fungi can suppress clubroot diseases incidence for 18.75%.

Control of Banana Wilt Disease Caused by Fusarium oxysporum Schlecht f.sp. cubense (E. F. Smith) Using Crab Shell Powder and Chitosan Widodo Widodo; Heri Harti; Suryo Wiyono
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2780

This research was conducted to evaluate the effectiveness of crab shell powder and chitosan to control Fusarium wilt disease on bananas and to analyze the involved control mechanisms. The effectiveness of crab shell powder and chitosan to F. oxysporum f.sp. cubense was examined in a laboratory (in vitro) and greenhouse (in planta). In vitro evaluation showed that chitosan has an antifungal effect while crab shell powder did not. Application of crab shell powder and chitosan suppressed the disease in green house test. The concentrations of crab shell powder and chitosan that most effective to control Fusarium wilt incidences were 0.25% and 0.10% with an efficacy rate of 66.7% and 83.3%, respectively. The highest disease severity reduction was showed by crab shell powder 0.25% and chitosan 0.50% with an efficacy rate of 56.8% and 59.4%, respectively. Suppression of the disease might be due to the fungicidal effect of chitosan and the increase of the total population of bacteria and chitinolytic bacteria in the rhizosphere when banana seedling roots were treated with crab shell powder or chitosan. Experiment results using the split roots technique exhibited the role of crab shell powder and chitosan potentially to induce the resistance of banana to Fusarium wilt.

Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR Fetrina Oktavia; Kuswanhadi Kuswanhadi; Dini Dinarty; Widodo Widodo; Sudarsono Sudarsono
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.881

The accession of the IRDB 1981 (PN’81) population is a newly introduced and an important rubber tree germplasm while the Wickham clone is a commercial variety one. The objectives of this study were to assess the genetic diversity and the population structure of PN’81 populations and the Wickham clones using 15 EST-SSR loci. Results of the analysis showed that the evaluated SSR primers yielded polymorphic markers. The gSSR 268 primer pairs yielded the most informative markers while HBE 280 primers generated the lowest ones. Results of the genetic diversity analysis supported that the PN’81 population belonged to a single large natural population of rubber trees while the Wickham clones belonged to a different group than that of PN’81. The population structure analysis of the rubber accessions was also in agreement with the results of the genetic diversity analysis. The experiment also indicated that PN’81 populations would be useful for future rubber breeding in Indonesia, especially as the sources of parent clones for rubber tree hybridization programs and rubber tree genetic resource conservation.

The Relationship Between Soil Fertility and Basal Stem Rot Disease in Oil Palm Plantations Evan Purnama Ramdan; Arief Hartono; Giyanto Giyanto; Sri Hendrastuti Hidayat; Widodo Widodo
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.384

In oil palm, Ganoderma boninense causes stem rot disease, which is often difficult to control, and soil fertility status is related to the ecology of G. boninense as a soil-borne pathogen. Therefore, this study aims to evaluate the relationship between soil fertility and stem rot disease as well as appropriate management methods to control the disease. This was carried out at the Nusantara Plantation Company's 7, Unit Kiwah Rejosari-Pematang from June 2021 to January 2022. The determination of observation blocks was carried out selectively using three blocks of land attacked by Ganoderma boninense with the same criteria for the year of planting and the same soil type. Each block consists of five plots. Each plot consisted of five sub-plots, consisting of 3 oil palms for disease severity assessment and soil sampling. The soil for each subplot was composed of 15 samples, which were analyzed for physical and chemical properties of the soil. Determination of fertility status based on the soil research manual published by the Indonesian Bogor Soil Research Center with parameters from the analysis results. The limiting factor for fertility is the cation exchange capacity of the soil, which ranges from 10.07 meq/100 g to 17.68 meq/100 g, and the C-organic content, which ranges from 0.40 to 1.15%. According to chi-square analysis, this fertility-limiting factor is related to disease severity. Therefore, management needs to be done by adding organic matter to the soil, practicing organic or inorganic fertilization, and following the principles of cultivating healthy oil palm plants.

Co-Authors ALI NURMANSYAH Ali Wafa Ana Feronika Cindra Irawati Andika Septiana Suryaningsih Andriani, Desta Anthoni Sulthan Harahap Asniah Asniah Bonjok Istiaji Busyairi Latiful Ashar Dedek Kusvianti devi ayu komalaningrat Dini Dinarty Dini Dinarty DJOKO PRIJONO DWI SUGIPRIHATINI Efi Toding Tondok Eryna Elfasari Rangkuti Evan P. Ramdan Fetrina Oktavia Fetrina Oktavia Firmansyah, Raden Arief Gayuh Rahayu Giyanto Giyanto Hari Priwiratama Heri Harti Hermanu Triwidodo Kikin H Mutaqin Kudang Boro Seminar Kuswanhadi Kuswanhadi Kuswanhadi Kuswanhadi Lia Marliyanti Lilih Naelun Najah Maggy Tenawidjaja Suhartono Maria Ulfa Putri Yoeshinda Mohamad Rahmad Suhartanto Muhamad Syukur Ni Putu Winda Mahasari Rahmi Sutrisni Ramdan, Evan Purnama SRI HENDRASTUTI HIDAYAT Sudarsono Sudarsono Sudarsono Sudarsono Sulastri Sulastri Supramana Suryo Wiyono Syaidatul Rosidah Titiek Siti Yuliani Umi Sallamatul Isniah Weni Wilia Yudi Sastro

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