Claim Missing Document
Check
Articles

Found 40 Documents
Search

MENGELOLA LEDAKAN HAMA DAN PENYAKIT PADI SAWAH PADA AGROEKOSISTEM YANG FRAGIL DENGAN PENGENDALIAN HAMA TERPADU BIOINTENSIF Suryo Wiyono; . Widodo; Hermanu Triwidodo
RISALAH KEBIJAKAN PERTANIAN DAN LINGKUNGAN Rumusan Kajian Strategis Bidang Pertanian dan Lingkungan Vol 1 No 2 (2014): Agustus
Publisher : Pusat Studi Pembangunan Pertanian dan Pedesaan (PSP3)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tingkat penggunaan pestisida pada padi sawah telah membuat agroekosistem padi sawah menjadi fragil, yang ditandai dengan makin seringnya ledakan hama dan penyakit pada 10 tahun terakhir di Pulau Jawa. Pengendalian Hama Terpadu Biointensif (PHT BI) merupakan suatu integrasi teknik terbaik (best practices) dalam pengelolaan hama dan penyakit padi didasari pada optimalisasi faktor pengendali hayati dan alami, serta pengelolaan kesehatan tanaman. Ujicobateknologi yang dikembangkan di enam lokasi pada enam kabupaten di Jawa menunjukkan bahwa PHT BI mampu mengendalikan hama dan penyakit serta meningkatkan produksi padi.
Eksplorasi dan Pengaruh Cendawan Endofit yang Berasal dari Akar Tanaman Cabai Terhadap Pertumbuhan Benih Cabai Merah (The Exploration and Effect of Endophytic Fungus Isolated from Chilli’s Root to Growth of Chilli Seedling) Anna Feronika Cindra Irawati; Kikin Hamzah Mutaqin; Maggy Tenawidjaja Suhartono; Yudi Sastro; nFN Sulastri; nFN Widodo
Jurnal Hortikultura Vol 27, No 1 (2017): Juni 2017
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v27n1.2017.p105-112

Abstract

Cendawan endofit diketahui memiliki kemampuan dalam menghasilkan metabolit sekunder yang sering berdampak terhadap pertumbuhan inangnya, seperti meningkatkan ketahanan tanaman terhadap kondisi cekaman biotik dan abiotik, maupun meningkatkan pertumbuhannya. Penelitian ini bertujuan untuk mengisolasi dan mengetahui pengaruh aplikasi cendawan endofit yang diperoleh dari akar tanaman cabai terhadap pertumbuhan bibit tanaman cabai besar (Capsicum annuum L.). Kegiatan penelitian dilakukan di Laboratorium Mikrobiologi dan Rumah Kaca BPTP Jakarta, sejak September 2012 hingga Agustus 2013. Penelitian terdiri dari eksplorasi, uji patogenisitas, dan uji efikasi terhadap cendawan endofit. Eksplorasi cendawan endofit dilakukan terhadap sampel akar tanaman cabai sehat dari daerah Garut (Jawa Barat), yang sering digunakan untuk budidaya tanaman cabai dan Cipayung (Jakarta Timur), yang merupakan daerah baru untuk budidaya tanaman cabai. Seleksi awal terhadap isolat cendawan dilakukan dengan uji patogenisitas secara in vitro. Uji efikasi cendawan secara in vivo pada bibit cabai berfungsi untuk mengetahui efek aplikasi cendawan terpilih (46 isolat non patogenik dan 16 isolat potensial patogenik) terhadap pertumbuhan bibit. Hasil eksplorasi menunjukkan bahwa isolat cendawan yang diperoleh dari akar tanaman cabai daerah Garut, secara makroskopis memiliki keragaman lebih tinggi dibanding dengan keragaman isolat dari daerah Cipayung. Uji patogenisitas menunjukkan bahwa isolat cendawan yang diuji cenderung didominasi oleh cendawan yang bersifat patogenik dan potensial patogenik. Uji efikasi cendawan endofit terpilih dalam mempengaruhi pertumbuhan vegetatif bibit, menunjukkan bahwa 74,19% isolat yang diuji memiliki kemampuan memicu pertumbuhan bibit pada kondisi media tanam steril. sebanyak 34 isolat dari isolat-isolat tersebut diketahui merupakan isolat yang sejak awal bersifat nonpatogenik dan 12 isolat yang awalnya bersifat potensial patogenik.KeywordsCendawan nonpatogenik; Metabolit sekunder; FitohormonAbstractEndophytic fungi are known be able to produce secondary metabolites that often impact on the growth of its host, such as increasing the resistance of plant to biotic and abiotic stress conditions, as well as enhance its growth. This study aimed to isolate and determine the effect of endophytic fungi applications that isolated from the roots of chilli plants to the growth of chilli seeds (Capsicum annuum L.). The research activities carried out in the Laboratory of Microbiology and Greenhouse BPTP Jakarta, from September 2012 to August 2013. The study was consisted the exploration, pathogenicity test, and the efficacy test of the endophytic fungi. The exploration of endophytic fungus carried out on samples of chilli healthy plant roots from the area of Garut (West Java), which is often used for the cultivation of chilli plants, and Cipayung (East Jakarta), which is a new area for the cultivation of chilli plants. The initial selection of the isolates of the fungi carried by the pathogenicity test in vitro. The efficacy test of the fungus, in vivo, in seedlings of chilli serves to determine the effect application of the fungus that selected on the growth of seedlings. Exploration results indicate that fungi isolates obtained from roots of pepper plants Garut, macroscopically has a higher diversity compared with isolates from regional diversity Cipayung. Pathogenicity test demonstrated that the fungi tested isolates tend to be dominated by a fungus that is pathogenic and potentially pathogenic. Test the efficacy of endophytic fungus chosen in influencing the vegetative growth of seedlings (46 isolates nonpathogenic and 16 isolates potentially pathogenic), showed that 74.19% of isolates tested has the ability to trigger the growth of seedlings at planting medium sterile conditions. As many as 34 isolates from isolates are well known consistence as nonpathogenic isolates and 12 isolates were to be potentially pathogenic isolates on pathogenicity test.
PERSPEKTIF PENGEMBANGAN KOMPOS BIOAKTIVATOR UNTUK PENGENDALIAN Phytophthora capsici PADA TANAMAN LADA (Piper nigrum) Jekvy Hendra; Widodo ;; Bonny P.W.S.; Bintoro H.M.; Manohara D.
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 17, No 1 (2014): Maret 2014
Publisher : Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v17n1.2014.p%p

Abstract

Perspective on the Development of Bio-Activator Compost to Control the Attacks of Phytophthora capsici on Pepper (Piper nigrum). Bio-activator microbes can be found enormously in the compost, for instance bacteria (Bacillus spp., Pseudomonas spp), actinomycetes (Streptomyces spp.) and fungi (Trichoderma spp, Aspergillus spp, Penicillium spp). This research aimed to assess the development of bio-activator in controlling P. capsici and increasing production and quality of the pepper. Activity was done in the field trials in the upland and lowland as the center of pepper plants in Lampung using a factorial randomized design group with five replications. The first factor was microbial bio-activator formula and the second factor was three doses of compost namely 1, 2 and 3 kg/pepper plants. Each plant was given zeolite, which was 0.5 kg/tt and fulfat acid 1% as amount of 200 cc/tt. The study showed that microbial activators significantly suppressed P. capsici attacks and increased the production of pepper. In the uplands, the intensity of P. capsici decreased to 83.31%, while in the lowlands, the disease intensity that was caused by P. capsici decreased by 99-100%. The results of chemical and biological analysis of soil showed that the application of bio-activator compost increased the diversity of the types and the amounts of microbes in the rhizosphere as well as increased the nutrient availability. Combination treatment of bio-activator compost from coffee leather of 3 kg/tt were able to suppress the intensity of the disease BPB to more than 50% and increase the production of more than 30 %. Key words: piper nigrum, bioactivator, coffee skin, Phytophthora capsicii. Di dalam kompos banyak ditemukan mikroba bioaktifator, yaitu bakteri (Bacillus spp., Pseudomonas spp), aktinomiset (Streptomyces spp.) dan cendawan (Trichoderma spp, Aspergillus spp, Penicillium spp.). Bioaktivator dapat dimanfaatkan untuk mengendalikan serangan P.capsicii dan meningkatkan produksi serta mutu hasil lada. Kegiatan ini bertujuan mengkaji pengembangan bioaktivator untuk pengendalian Phytopthora capsici dan peningkatan produksi serta  mutu hasil lada. Pengkajian dilakukan dalam format uji lapangan didataran tinggi dan rendah sentra lokasi tanaman lada di Lampung. Pengkajian menggunakan Rancangan Acak Kelompok pola faktorial dengan lima ulangan. Faktor pertama ialah  bentuk formula  mikroba bioaktivator dan faktor kedua ialah tiga dosis kompos yaitu 1,2 dan 3 kg/tanaman lada. Setiap tanaman juga diberikan  zeolit 0.5 kg/tt dan asam fulfat 1% 200 cc/tt. Hasil pengkajian menunjukkan bahwa mikroba aktivator nyata menekan serangan P.capsicidan meningkatkan produksi lada. Di dataran tinggi intensitas serangan P. capsicimenurun hingga 83.31%, sedang  di dataran rendah intensitas serangan P capsicimenurun hingga 99-100%. Hasil analisis kimia tanah dan mikroba tanah menunjukkan bahwa aplikasi kompos bioaktivator meningkatkan keragaman jenis dan jumlah mikroba di dalam rhizosfer serta meningkatkan ketersediaan hara. Kombinasi perlakuan bioaktivator dan kompos kulit kopi 3 kg/tt mampu menekan intensitas penyakit BPB hingga > 50% dan meningkatkan produksi > 30%.   Kata kunci: Piper nigrum,  bioaktivator, kulit kopi, Phytopthora capsicii.
EKSPLORASI CENDAWAN ENDOFIT DARI TANAMAN CABAI YANG BERPOTENSI SEBAGAI AGENS BIOKONTROL PENYAKIT ANTRAKNOSA (Colletotrichum acutatum L.) (Exploration of Endophytic Fungi from Pepper as Biological Control Agents of Anthracnose (Colletotrichum acutatum L.) Weni Wilia; . Widodo; Suryo Wiyono
Bioplantae Vol. 2 No. 1 (2013)
Publisher : Bioplantae

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Anthracnose caused by Colletotrichum acutatum is one of devastated disease of pepper in Indonesia and has great impact on yield loss. Most of farmers use fungicides as controller for this disease. Application of biocontrol agents could be solution to control anthracnose. Beneficial microorganism, such as endophytic fungi are able to be promising biocontrol agents of some pathogens, including Colletotrichum acutatum. The aim of this research was to isolate endophytic fungi from fruit and branch as biocontrol agents for pepper anthracnose. The research resulted 11 isolates of endophytic fungi that were further tested against anthracnose. Isolates of endophytic fungi were identified as steril hifa hitam 1, steril hifa hitam 2, steril hifa 1, steril hifa 2, Nigrospora sp., Coniotyrium sp., Cylindrocarpon sp., Paecilomyces sp. The 3 of other isolates (isolat buah 5, isolat buah 1dan isolat batang 24) were not identified yet.   Keyword : Pepper, antrachnose, C. acutatum, endophytic fungi
COMBINING ABILITY AND HETEROSIS OF CHILLI (Capsicum annuum) RESISTANCE TO ANTHRACNOSE Yulia Irawati; Sriani Sujiprihati; Widodo -
Widyariset Vol 15, No 3 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.476 KB) | DOI: 10.14203/widyariset.15.3.2012.683–690

Abstract

Anthracnose (Colletotrichum acutatum) is considered as the major disease in chilli. Resistant variety isimportant for controlling infection of disease, since fungicide application and other cultural controlled methodswere not effective. The aim of this research was to estimate combining ability and heterosis of chilli resistanceto anthracnose. Set population of half diallel crosses which involved 6 parents and 15 crosses with C. acutatumisolate BKT 04 were used in this experiment. Mature green fruit were inoculated using a microinjection method.Disease resistant percentage and lesion diameter were used for estimating of combining ability. Disease incidenceand lesion diameter were used for estimating heterosis. General combining ability of IPB C15 was high for diseaseresistance percentage and lesion diameter. Specific combining ability of IPB C15 x IPB C9 was high for diseaseresistance percentage and specific combining ability of IPB C15 x IPB C10 and IPB C4 x IPB C2 were high forlesion diameter. Negative heterosis was expected in disease resistance traits; IPB C15 x IPB C9 had negativeheterosis for disease incidence and IPB C10 x IPB C9 had negative heterosis for lesion diameter.
Fusarium Species Associated with Corm Rot of Taro in Bogor . WIDODO; . SUPRAMANA
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.153 KB) | DOI: 10.5454/mi.5.3.6

Abstract

In Bogor-West Java, corm rot disease of taro has devastated in many cropping areas and caused yield losses up to 70%. During preliminary studies, Fusarium species were constantly recovered when diseased materials, rotten corms and discolored petioles were incubated. The objectives of this study was to identify the Fusarium species associated with the corm rot disease in Bogor and determine their pathogenicity and hosts range. Samples were collected from 40 diseased corm samples taken in 9 sub-districts of Bogor. Two species, viz, Fusarium solani and F. oxysporum were identified based on the morphological characteristics. Among 40 Fusarium isolates recovered in this study, 70% were Fusarium solani and 30% were F. oxysporum.  F. solani could infect to all tested edible Araceae , while F. oxysporum was only pathogenic to Colocasia esculenta. Both species of Fusarium did not cause any symptoms when inoculated on selected ornamental and legume crops. These results gave the indication that F. solani was probably pathogenic only to edible Araceae, but further inoculation assay on living taro plants are necessary to carry out in order to clarify this result.
Selection of Yeasts Antagonists as Biocontrol Agent of Mango Fruit Rot caused by Botryodiplodia theobromae DWI SUGIPRIHATINI; SURYO WIYONO; . WIDODO
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.9 KB) | DOI: 10.5454/mi.5.4.2

Abstract

Fruit rot caused by Botryodiplodia theobromae is one of the most important post harvest disease of mango in Indonesia. Study on biological control on the disease is required to develop environmentally-sound control technology.  The research objectives were to study the potency of yeasts in controlling post harvest mango disease i.e. fruit rot caused by B. theobromae and mechanism involve in the biocontrol. Total yeast isolates used for screening were twenty one, four from collection of Plant Clinic of Institut Pertanian Bogor, and twenty one isolated from healthy mango skin. All of yeast isolates were characterized and identified using BIOLOG. Bioassay on antagonistic activity of yeasts against fruit rot, in-vitro dual culture test and chitinolytic activity were carried out.  Cryptococcus albidus var. aerius WSW1, Pichia guilliermondii K1, and Debaryomyces hansenii K12 were the three most effective antagonistic yeasts against B. theobromae with effectiveness of 70.83%, 45.83%, 37.50% respectively. In vitro bio-assay showed that C. albidus var. aerius WSW1, C. albidus WSW2, C. albidus K6, C. terreus YSW1, Candida edax OSW1, Candida edax K13, and Cryptococcus luteolus K2 had high antibiosis activity.  Biocontrol activity of tested yeasts against fruit rot of mango did not correlate to its antibiosis and chitinolytic activity
Penggunaan Vis-NIR untuk Deteksi Serangan Huanglongbing pada Daun Jeruk Raden Arief Firmansyah; Kudang Boro Seminar; Widodo .
Jurnal Keteknikan Pertanian Vol. 5 No. 1 (2017): JURNAL KETEKNIKAN PERTANIAN
Publisher : PERTETA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1684.301 KB) | DOI: 10.19028/jtep.05.1.%p

Abstract

AbstractHuanglongbing is citrus disease which is a major threat for citrus orchard. Neither disease has a cure nor an efficient means of control. Early detection is important to prevent development and spread of the disease. The most effective detection used DNA test by PCR. However, identification used DNA test required sample preparation, time-consuming and expensive. The objective of this study was to build detection of healthy and HLB-infected leaves software. The leaf samples collected from citrus orchard in Situgede village, Bogor. Sampleleaves divided into three group, Huanglongbing-infected leaves, healthy leaves and asymptomatic leaves. All samples was tested by PCR for verification visual symptoms of huanglongbing. Vis-NIR spectrometer with a spectra range of 339 to 1022nm was used to acquisition HLB-infected and healthy leaves spectral data. MSC, SNV, baseline correction, first and second derivative were used for pretreatment method. Artificial neural network was used to build classification model. X-loading plot from principal component analysis was used to obtain sensitive wavelength. Classification for healthy and HLB-infected classs used sensitive wavelength baseline correction-based had the best performance and high accuracy (100%). The classification model was embedded in software PC-desktop based which was used visual basic programming language. Asymptomatic leaves spectral from HLB-positive tree were used to testing classification model. Model classified data into HLB-infected group, which was consistent with PCR test. The result from this study indicated that developed software could be used to HLB detection in early stage of disease.AbstrakHuanglongbing adalah penyakit jeruk yang merupakan ancaman utama bagi budidaya jeruk. Tidak ada pengendalian yang tepat untuk Huanglongbing. Deteksi dini penting untuk mencegah penyebaran dan pengembangan penyakit ini. Deteksi dini yang paling efektif menggunakan tes DNA dengan PCR. Namun, identifikasi menggunakan tes DNA memerlukan persiapan sampel, memakan waktu dan mahal. Tujuan daripenelitian ini adalah membangun perangkat lunak deteksi daun sehat dan terinfeksi HLB. Sampel daun dikumpulkan dari kebun jeruk di Desa Situ Gede, Bogor. Sampel daun dibagi menjadi tiga kelompok, daun yang terinfeksi HLB, daun sehat dan daun belum bergejala. Semua sampel telah diuji dengan PCR untuk verifikasi gejala visual Huanglongbing. Spektrometer Vis-NIR dengan rentang spektrum dari 339-1022nm digunakanuntuk mengumpulkan data spektrum daun terinfeksi HLB dan sehat. MSC, SVN, baseline correction, turunan pertama dan kedua dari spektra digunakan sebagai metode praperlakuan. Jaringan syaraf tiruan digunakan untuk membangun model klasifikasi Plot X-loading dari analisis komponen utama digunakan untuk mendapatkan panjang gelombang sensitif. Klasifikasi terhadap kategori daun sehat dan sakit menggunakan panjang gelombang sensitif berbasis baseline correction memiliki nilai akurasi 100 % dan kinerja terbaik. Model klasifikasi yang ditanam pada perangkat lunak berbasis komputer desktop menggunakan bahasa pemrograman visualbasic. Data spektrum daun belum bergejala dari pohon positif terinfeksi HLB digunakan untuk menguji model klasifikasi. Model mengklasifikasikan data tersebut ke kelompok terinfeksi HLB, yang konsinten dengan hasil pengujian PCR yang juga mengelompokkan pada daun terinfeksi HLB. Hasil penelitian ini menunjukkan bahwa perangkat lunak dapat digunakan untuk deteksi HLB pada tahap awal perkembangan penyakit.
IDENTIFIKASI KETAHANAN PLASMA NUTFAH KARET IRRDB 1981 TERPILIH TERHADAP PENYAKIT GUGUR DAUN CORYNESPORA BERDASARKAN AKTIVITAS TOKSIN CASSICOLIN Fetrina Oktavia; Sudarsono Sudarsono; Kuswanhadi Kuswanhadi; Dini Dinarty; Widodo Widodo
Jurnal Penelitian Karet JPK : Volume 34, Nomor 1, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i1.225

Abstract

Salah satu penyakit pada tanaman karet adalah penyakit gugur daun Corynespora (PGDC) yang disebabkan oleh jamur Corynespora cassiicola. Patogen tersebut dapat menyerang semua tahap pertumbuhan tanaman karet yang menyebabkan terjadinya penurunan hasil lateks yang cukup signifikan dan bahkan dapat menyebabkan kematian tanaman. Penggunaan klon-klon tahan sebagai bahan tanam merupakan cara yang paling efektif dan ekonomis untuk mencegah terjadinya serangan PGDC. Karena itu identifikasi klon-klon resisten merupakan strategi utama dalam manajemen PGDC. Penelitian ini bertujuan untuk mengidentifikasi tingkat ketahanan 50 genotipe terpilih plasma nutfah IRRDB 1981 (PN’81) terhadap PGDC. Enam klon karet yang berasal dari populasi Wickham digunakan sebagai pembanding (BPM 24, BPM 1, GT 1, RRIC 600, PB 260 dan RRIM 600). Empat isolat C. cassiicola (CC-01, CC-20, CC-22, dan CC-23) diinokulasikan masing-masing pada daun muda tahap pertumbuhan B2C, dan intensitas kelayuan daun dihitung berdasarkan estimasi kehilangan air akibat aktifitas patogenisitas toksin. Hasil penelitian menunjukkan bahwa 12 genotipe tergolong sangat tahan, 13 genotipe tergolong tahan, 23 genotipe tergolong rentan dan 8 genotipe tergolong sangat rentan. Genotipe PN 451, PN 494 dan PN 604 menunjukkan tingkat ketahanan yang lebih baik terhadap PGDC dibandingkan dengan populasi Wickham sehingga ketiga genotipe tersebut berpotensi digunakan sebagai sumber gen ketahanan dalam program pemuliaan tanaman karet. Diterima : 13 Maret 2016 / Direvisi : 29 Juli 2016 / Disetujui : 2 Agustus 2016 How to Cite : Oktavia, F., Sudarsono, S., Kuswanhadi, K., Dinarty, D., & Widodo, W. (2016). Identifikasi ketahanan plasma nutfah karet IRRDB 1981 terpilih terhadap penyakit gugur daun Corynespora berdasarkan aktivitas toksin Cassicolin. Jurnal Penelitian Karet, 34(1), 35-48. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/225
PENGARUH PH DAN WAKTU PENYIMPANAN TERHADAP PERTUMBUHAN Lasiodiplodia theobromae PENYEBAB KANKER BATANG TANAMAN KARET Tri Rapani Febbiyanti; Widodo Widodo; Suryo Wiyono; Sudirman Yahya
Jurnal Penelitian Karet JPK : Volume 37, Nomor 1, Tahun 2019
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v37i1.615

Abstract

Lasiodiplodia theobromae merupakan cendawan penyebab kanker batang pada tanaman karet (Hevea brasiliensis Muel Arg.), penyakit ini merupakan penyakit baru dan belum pernah dilaporkan sebelumnya di Indonesia.  Banyak cara yang dapat dilakukan untuk mengendalikan penyakit kanker batang. Strategi pengendalian yang terbaik adalah dengan pengelolaan penyakit yang membutuhkan pengetahuan mengenai ekobiologi patogen, inang, dan interaksinya dengan lingkungan biotik dan abiotik. Pengendalian patogen harus didasarkan pada pengetahuan tentang bioekologinya. Diharapkan dengan pengetahuan bioekologi bisa dijadikan dasar untuk bisa menghambat perkembangan atau menurunkan populasi inokulum di lapangan. Penelitian ini bertujuan untuk menentukan pengaruh pH secara in vitro terhadap pertumbuhan patogen kanker batang dan laju penurunan viabilitas tubuh buah akibat penyimpanan.  Kisaran pengaruh pH media meliputi 3, 4, 5, 6 dan 7. Penyimpanan tubuh buah dilakukan dalam wadah tanpa diberi tanah, diberi tanah steril dan tanah tidak steril. Penentuan viabilitas dilakukan pada 30, 60, 90 dan 120 hari masa penyimpanan. Semakin rendah pH menyebabkan pertumbuhan miselia terhambat.