Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

0.444

P-Index

This Author published in this journals

All Journal Buletin Agrohorti Jurnal Fitopatologi Indonesia Bioplante Microbiology Indonesia Jurnal Keteknikan Pertanian AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian

Widodo Widodo

Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Kamper Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 2432568

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology

Published : 29 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

Deteksi dan Identifikasi Cendawan Terbawa Benih Brassicaceae Anthoni Sulthan Harahap; Titiek Siti Yuliani; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed quality is very critical in agricultural production, especially to gain high yield and reduce disease problems in the field. New diseases or pathogens is potentially entering a country through seed movement by import activity. This study aimed to detect and identify seed-borne fungi from Brassicaceae seeds imported from the United States and Malaysia. Seeds were incubated on 5 sheets of wet blotting paper at a temperature of 27–30 °C for 14 days following surface sterilization. Each fungus that grows on the seed was isolated on potato dextrose agar and malt extract agar for further morphological identification. The three fungi most commonly found either on the seed with or without surface-sterilization were Aspergillus flavus, Curvularia lunata and A. niger. All of the fungi were a potential pathogen in the family Brassicaceae seeds and seedlings. Important pathogen in Brassicaceae crops, i.e. Phoma lingam was also found in small amounts and only on white pak choy seeds.

Pengendalian Colletotrichum spp. Terbawa Benih Cabai dengan Paparan Gelombang Mikro Lilih Naelun Najah; Mohamad Rahmad Suhartanto; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed treatment using microwave has been reported as an effective and efficient method to control seed borne pathogens of chili pepper. The purpose of this research was to evaluate the effectiveness of microwave irradiation treatment to suppress seedborne Colletotrichum spp. while maintaining physiological quality of chili’s seed. First experiment was aimed to select the best condition for seed germination, and was done in completely randomized design with three levels of water content (4.31%, 6.33%, and 8.25%). The second experiment was aimed to determine the best condition for disease suppression, and was done in completely randomized design with different levels of microwave irradiation duration (0, 10, 20, 30, 40, and 50 seconds) using the best seed water content level from the first experiment. Application of systemic fungicide with benomyl as active ingredient (0.5 g L-1) was done as check treatment. Four major species of Colletotrichum was found from chili’s seed, i.e. C. acutatum, C. capsici, C. gloeosporioides, and Colletotrichum sp. with C. acutatum as the predominant species. The best water content level for microwave treatment was 4.31%. The most effective treatment was microwave irradiation duration for 40 seconds with efficacy rate of 64.5% for controlling seedborne C. acutatum on chili pepper.

Cendawan Endofit yang Potensial Meningkatkan Ketahanan Cabai Merah terhadap Penyakit Layu Bakteri Ana Feronika Cindra Irawati; Yudi Sastro; Sulastri Sulastri; Maggy Tenawidjaja Suhartono; Kikin Hamzah Mutaqin; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Ralstonia solanacearum has been known to cause bacterial wilt disease on chili pepper. Despite many reports on the potential use of endophytic fungi to induce plant resistance, its utilization to suppress bacterial wilt disease of chili has not been widely reported. The aims of this research was to screen potential endophytic fungi that may increase chili resistance against bacterial wilt disease. Selection of endophytic fungi was done using in vivo and antibiosis test. Strains of fungi were considered the most potent in suppressing the development of bacterial wilt in chilli were identified. Ten out of 62 isolates of endophytic fungi gave the highest suppression on chilli’s bacterial wilt disease. Most strains of endophytic fungi were able to suppress the development of bacterial wilt disease, but not always positively correlated to the vegetative and generative growth of chilli. Based on the level of disease intensity and the growth of plants were obtained three strains of endophytic fungi that considered potentially suppress the incidence of bacterial wilt disease. The three isolates was identified as Fusarium solani f.sp. phaseoli (AC-2.13 and AC-4.4) and Trichoderma asperellum (AC-3.18) using morphology and molecular characters. Although all three selected isolates were able to suppress bacterial wilt disease in this study, but application of F. solani f.sp. phaseoli should be considered in practical use since it is generally known as the causal agent of root rot disease of beans

Sensitivitas Colletotrichum spp. pada Cabai terhadap Benomil, Klorotalonil, Mankozeb, dan Propineb Desta Andriani; Suryo Wiyono; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Anthracnose caused by several species of Colletotrichum is one of limiting factors for chilli production. Up to now the control of antrachnose disease still rely on regular and intensive application of synthetic fungicides. Application of similar active ingredients of synthetic fungicides may create resistance of the pathogens. This study was conducted to evaluate the sensitivity of three species of Colletotrichum against four commercial fungicides with different active ingredients, i.e. benomyl, chlorotalonil, mancozeb, and propineb. The sensitivity level and the potential occurrence of resistance of Colletotrichum were observed based on relative inhibition rate using poisoning food method. The results showed that all isolates were highly resistant to chlorotalonil, even up to ten times of the highest recommended concentrations. The tested fungal isolates were resistant or highly resistant to the recommended concentrations of mancozeb and propineb, but they were still sensitive when the concentrations were increased up to five times. Isolates of Colletotrichum spp. showed sensitive to highly sensitive response to benomyl.

Potensi Cendawan Endofit sebagai Pengendali Hayati Penyakit Busuk Pangkal Batang (Phytophthora capsici) pada Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Stem rot disease caused by Phytophthora capsici is an important disease on chilli. Eight endophytic fungi that had been isolated and screened based on pathogenecity test were further tested for their potential as the biological control agent of the stem rot disease of chilli. The endophytic fungi suspension was applied twice during the trial. The first application was on 100 seed lot, by soaking them in 100 mL of suspension. The second application was on the 3 weeks-old chili seedlings by drenching them with 10 mL suspension per plant. The concentration of endophytic fungi in the suspension was 2.8 × 106 cfu mL-1. The disease intensity and AUDPC value were measured for 4 weeks after the pathogen inoculation. The growth inhibition test of P. capsici was performed in vitro and the colonization abilities of endophytic fungi were observed at 4 weeks-old chilli seedlings. Eight endophytic fungi inhibited the growth of the P. capsici, and two of those isolates namely Penicillium strain MAG1 and Penicillium strain PAB2 showed antibiosis mechanism. Endophytic fungi has the ability more to colonize at the root (26–60%) than in the stem (20–40%). Fusarium strain MAGR1 has the highest level of endophytic colonization i.e. 60% compared to others. Based on in vivo assay, six endophytic fungi isolates, i.e. Fusarium strain MAGR1, Penicillium strain MAG1, Penicillium strain PAB2, sterile hyphae HAJ1, sterile hyphae HAJ2, and sterile hyphae PBG7, showed the potency to control stem rot disease with inhibition level of 25.5–35.5%

Identifikasi Colletotrichum spp. Asal Tanaman Pepaya Eryna Elfasari Rangkuti; Suryo Wiyono; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Colletotrichum spp. is known as the causal agent of anthracnose and considered as an important limiting factors on papaya production. The objective of this study was to determine the species of Colletotrichum spp. from various plant parts of papaya (Carica papaya) showing anthracnose symptom. Twenty isolates of Colletotrichum spp. was isolated and were grouped into 3 morphological groups based on colony similarity, conidial morphology, and setae formation. A total of 12 isolates of group I and 3 isolates of group II were identified morphologically as C. gloeosporioides sensu lato based on cylindrical conidia with rounded on both ends and colony morphology. Meanwhile, 1 isolate of group II was molecularly identified as C. magnum. Four isolates of group III with a curved and pointed-end conidia, and produced setae on artificial medium were identified molecularly as C. truncatum. C. gloeosporioides was obtained on stem, leaf petiole, and fruit. C. magnum and C. truncatum were only obtained on leaf petiole and fruit, respectively. In addition to morphological and molecular characters, differences in colony growth responses to temperature can be used to distinguish the species of Colletotrichum. To our knowledge the existence of anthracnose symptom on the stems and leaf petioles of papaya in the fields and the discovery of C. magnum and C. truncatum isolated from papaya was the first report in Indonesia.

Identitas Spesies Botrytis pada Tanaman Hortikultura Di Jawa Barat, Indonesia devi ayu komalaningrat; Efi Toding Tondok; widodo widodo
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Identity of Botrytis Species on Horticultural Crops In West Java, IndonesiaBotrytis species are economically important pathogens with a very broad host range including more than 200 horticultural crops. The identity of these fungus found in Indonesia has not been investigated and need to be reconfirmed due to the species variations of Botrytis found worldwide. The aims of this research were to identify Botrytis species infecting crops in West Java based on its morphology and molecular characteristics, as well as its pathogenicity traits. Based on morphological characters, all 25 isolates found were identified as B. cinerea. ITS-based sequences of the 8 isolates showed 96-100% similarity to reported B. cinerea in GenBank. The phylogenetic analysis confirmed that all collected B. cinerea were grouped in the same cluster with Australia, Netherlands, and other Asian region isolates. Pathogenicity tests using strawberry fruits demonstrated that all isolates were pathogenic as indicated by grey mold symptom development; the isolates from orchid showed the highest virulence. This research is the first report confirming Botrytis cinerea identity based on morphology and molecular methods in Indonesia, and also confirmed B. cinerea as the only species of Botrytis found in West Java.

Kanker Batang: Penyakit Baru pada Kopi di Lampung Suryo Wiyono; Andika Septiana Suryaningsih; Ali Wafa; Efi Toding Tondok; Bonjok Istiaji; Hermanu Triwidodo; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Stem Canker: A New Disease of coffee in LampungStem cancer is a new disease that has attacked smallholder coffee plantations in Lampung since 2010. The cause of the disease was unknown. This study aims to describe the symptoms of the disease, the incidence of the disease in the affected plantation, and identify morphologically and molecularly the canker pathogens of the coffee stem canker diseases. All stages of Koch’s postulate were carried out in laboratories and greenhouses. The isolated pathogens were morphologically characterized by colony shape and color as well as the conidia shape and size. Molecular identification was carried out by using a general primer (ITS1 and ITS4) and followed by sequencing. The main symptoms of the disease are stem cancer and dieback, as well as more infecting older plants. Pathogen of the coffee stem canker disease that attacks coffee plants in Lampung has been identified as Fusarium solani which has 99% homology with F. solani KY245947.1.

Identifikasi Infraspesifik Fusarium oxysporum asal Subtrat Nonpisang dan Kemampuan Pindah Inangnya ke Tanaman Pisang Gayuh Rahayu; Widodo Widodo; Ni Putu Winda Mahasari
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Infra-specific Identification of Fusarium oxysporum from Nonbanana Substrates and Its Ability to Move Hosts to Banana PlantsFusarium oxysporum has various life style, i.e. saprobe, endophyte and pathogen. Plant pathogenic F. oxysporum are divided into many forma specialis (f. sp.) depending on the host, for instance F. oxysporum f. sp. cubense (Foc), a causal agent of Panama disease of banana. The study aimed to determine the infraspecific identity of F. oxysporum from non-banana host and evaluate its ability to jump banana plants. Infraspecific identity was determined through a molecular approach using 3 specific primers to recognize TR4 race (TR4 F/R, TR4 F/R1, and FocSc-1/ FocSc-2), while the host’s transfer ability was tested on 2 banana cultivars namely cv. Ambon and Tanduk. Thirteen strain studied i.e IPBCC 88,012, IPBCC 07,528, IPBCC 07,561, IPBCC 08,562, IPBCC 08,568, IPBCC 10.674, IPBCC 14.1236, IPBCC 14.1237, IPBCC 14.1238 and IPBCC 14.1239 were TR4 Foc; IPBCC 07,338 and IPBCC 14.1242 are race Foc 4. The pathogenicity test of Foc IPBCC 88,012, 07,328, 08,561, 10,674 and 14.1236 derived from cucumbers, soil, agarwood sapwood, insect nests and quinine tree endophytes showed that these strains were able to move to banana plant. This shows that Foc may not host-specific and the infaspecific term forma specialis is therefore debatable.

Simulasi Sebaran Penyakit Blas Pada Tanaman Padi Menggunakan Model Spatial Multi Criteria Evaluation: Studi Kasus Kabupaten Karawang dan Purwakarta Busyairi Latiful Ashar; Ali Nurmansyah; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 15 No 2 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Dispersal Simulation of Rice Blast Disease Using Spatial Multi Criteria Evalution Model: Case Study In District of Karawang and PurwakartaRice blast is caused by Pyricularia oryzae. The potential epidemic of this disease can be spatially simulated using the MCA (Multi Criteria Analysis) method based on geographical characteristics, cultivation practices, and eviromental condition. A software that can be used for MCA is SMCE (Spatial Multi Criteria Evaluation). This study was aimed to predict the spatial dispersal of blast disease using SMCE model, and identify the factors that supports the epidemic. The study was conducted in February - August 2018 in Karawang and Purwakarta District. The research methods include observing the severity of blast disease, cultivation practices and environmental conditions, and analyzing SMCE. The SMCE analysis uses rice crop maps from the Sistem Monitoring Pertanaman Padi (Simotandi), which consists of grouping factors, standardizing factors, and weighting factors. The SMCE results are a simulation map of blast disease dispersal which is then compiled with predictions of its severity. Accuracy of prediction results was evaluated by MAPE (Mean Absolute Percentage Error) based on observational data on actual disease severity. The prediction results for Karawang and Purwakarta showed means of accuration 78.16% and 73.95% respectively. In general, factors that have a strong influence on the development of blast disease include altitude, distance from source of the epidemic, history of disease in the fields, number of spores (inoculum) trapped, irrigation quality, application of herbicides, soil nutrient (N, P, K) contents and the level of soil acidity.

Co-Authors ALI NURMANSYAH Ali Wafa Ana Feronika Cindra Irawati Andika Septiana Suryaningsih Andriani, Desta Anthoni Sulthan Harahap Asniah Asniah Bonjok Istiaji Busyairi Latiful Ashar Dedek Kusvianti devi ayu komalaningrat Dini Dinarty Dini Dinarty DJOKO PRIJONO DWI SUGIPRIHATINI Efi Toding Tondok Eryna Elfasari Rangkuti Evan P. Ramdan Fetrina Oktavia Fetrina Oktavia Firmansyah, Raden Arief Gayuh Rahayu Giyanto Giyanto Hari Priwiratama Heri Harti Hermanu Triwidodo Kikin H Mutaqin Kudang Boro Seminar Kuswanhadi Kuswanhadi Kuswanhadi Kuswanhadi Lia Marliyanti Lilih Naelun Najah Maggy Tenawidjaja Suhartono Maria Ulfa Putri Yoeshinda Mohamad Rahmad Suhartanto Muhamad Syukur Ni Putu Winda Mahasari Rahmi Sutrisni Ramdan, Evan Purnama SRI HENDRASTUTI HIDAYAT Sudarsono Sudarsono Sudarsono Sudarsono Sulastri Sulastri Supramana Suryo Wiyono Syaidatul Rosidah Titiek Siti Yuliani Umi Sallamatul Isniah Weni Wilia Yudi Sastro

Title

Found 29 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 29 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 29 Documents
Search