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SKRINING RHIZOBAKTERIA PENGHASIL INDOLE ANCETID ACID PENGINDUKSI PERTUMBUHAN DARI RHIZOSFIR CABAI (Capsicum annuum L.) Jumsu Trisno
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Rhizobacteria was a colonialist bacterial at rooting area that had capacity to produce IAA and enhance plant growth. Rhizobacteria indigenus chili had ability to produce IAA and induces various of plant growth. IAA production was not always linked with the ability to enhance plant growth. Obtained there were 11 isolates with various of morphological characters and had ability as RMSD (Plant Growt Promoting Rhizobacteria) with an effective increase in growth from 57.88 to 82.80%.Key words: Rhizobacteria, IAA, Growth, Chili Rhizosfer

DISEMINASI TEKNOLOGI PUPUK KANDANG SAPI PLUS RIZOBAKTERI PADA KELOMPOK TANI KAKAO DI KABUPATEN LIMAPULUH KOTA Haliatur Rahma; Jumsu Trisno; Martinius Martinius; Reflin Reflin; Sri Wahyuni; Nusyirwan Nusyirwan
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.158 KB)

Kegiatan diseminasi teknologi pupuk kandang sapi plus rizobakteri pada tanaman kakao dilakukan di Kecamatan Guguak dan Akabiluru Kabupaten Lima Puluh Kota yang dimulai dari bulan Maret sampai dengan November 2018. Kegiatan ini bertujuan untuk mengenalkan teknologi formulasi pupuk kandang plus rizobakteri pada kelompok tani kakao. Inovasi, buah lobek, maju sejahtera dan aroma menggunakan metode penyuluhan. Pentingny kegiatan ini dilakukan disebabkan oleh tingginya kejadian dan keparahan penyakit VSD di Kecamatan Guguak dan Akabiluru Kabuparten Limapuluh Kota yang mencapai 86,60%. Untuk itu, diperlukan teknologi pengendalian yang mudah dibuat, murah dan bahan baku yang mudah diperoleh disekitar petani. Penggunaan air kelapa merupakan salah satu perbanyakan bakteri Serratia maresescens dan Pseudomonas fluorescens yang nanti akan diformulasi dengan pupuk kandang. Kegiatan ini meliputi: pemantauan tingkat serangan OPT dilahan kelompok tani kakao, kemudian dilanjutkan dengan penyuluhan, pelatihan, dan evaluasi hasil kegiatan. Dari hasil kegiatan bahwa kegiatan ini mampu meningkatkan pengetahuan petani terhadap penggunaan biofertilizer yang berasal dari bakteri yang diformulasikan dengan air kelapa dan pupuk kandang, sehingga penggunaan formulasi ini mampu mengurangi biaya penggunaan pupuk kimia yang memiliki harga yang mahal.

Infeksi Ganda Pepper Yellow Leaf Curl Virus dan Chilli Veinal Mottle Virus dalam Menimbulkan Penyakit Daun Kuning Keriting Cabai Jumsu Trisno; Jamsari Jamsari; Sri Hendrastuti Hidayat
Jurnal Proteksi Tanaman Vol 5 No 2 (2021): December 2021
Publisher : Plant Protection Departement, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.

DISEMINASI PGPR SEBAGAI BIOAKTIVATOR DEKOMPOSISI BAHAN ORGANIK DI KELOMPOK USAHA TANI NAGARI SUNGAI ABANG KECAMATAN LUBUK ALUNG KABUPATEN PADANG PARIAMAN Haliatur Rahma; Indra Dwipa; Agustian Agustian; Yaherwandi Yaherwandi; Reflinaldon Reflinaldon; Jumsu Trisno; Armansyah Armansyah; Gusmini Gusmini; Afrima Sari; Ryan Dwi Setyawan; Rika Hariance; Dede Suhendra
Jurnal Hilirisasi IPTEKS Vol 6 No 2 (2023)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v6i2.625

Nagari Sungai Abang is one of the areas in Lubuk Alung District, one of the rice production centers in Padang Pariaman Regency. One farming group that intensively cultivates rice is the Farming Business group. The problem with the farmer groups is their ignorance of the benefits of straw as a source of organic matter in the soil. The composting process is long, so farmers always burn rice straw after harvest in preparation for planting the following season. If the burning of straw is carried out continuously, it is feared that nutrients will be deficient in the soil. This activity aims to empower Farming Business groups in Nagari Sungai Abang, Lubuk Alung District, to utilize Plant Growth Promoting Rhizobacteria (PGPR) as a bio activator in making straw compost as organic fertilizer and an alternative to addressing the problem of straw waste in Nagari Sungai Abang, Lubuk Alung. This activity has been carried out in the Farming Group of Farmers in Sungai Abang Dalam Nagari Sungai Abang, Lubuk Alung District, Padang Pariaman Regency. The method used is outreach, counseling, and training to demonstrate making PGPR suspension and compost from straw. From the activities that have been carried out, ready-to-use PGPR is obtained as a bio activator reproduced in coconut water media and used as a bio activator to speed up the composting process. The conclusion of this activity is increasing farmers' understanding of the benefits of straw as a raw material for compost and the use of PGPR as a bioactivator in making straw compost.

The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra Ridho, Muhammad Arif; Martinius; Fadli; Liswarni, Yenny; Najmi, Lailatul; Trisno, Jumsu
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.183-187

Tanaman mentimun varietas lokal Padang merupakan tanaman sayuran unggulan bagi petani perkotaan karena umur panen yang pendek. Hasil survei menemukan adanya gejala bercak kuning dan klorosis dengan tulang daun hijau. Gejala ini mirip dengan infeksi Papaya ring spot virus (PRSV) yang menginfeksi mentimun di Jawa dan Zucchini yellow mosaic virus (ZYMV) yang menginfeksi mentimun pada umumnya. Deteksi virus dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan primer spesifik PRSV dan primer universal Potyvirus, perunutan dan analisis DNA. RT-PCR menggunakan primer spesifik PRSV DNA target tidak menghasilkan pita DNA, namun pita DNA dengan ukuran ±320 pb berhasil teramplifikasi dengan primer universal Potyvirus. Analisis sikuen nukleotida menunjukkan bahwa similaritas virus tertinggi sebesar 93.8% dan 93.5%, berturut-turut dengan ZYMV isolat Singapura dan India. Infeksi ZYMV merupakan kejadian pertama pada tanaman mentimun di Padang, Sumatera Barat.

POTENSI RIZOBAKTERI INDIGENUS SEBAGAI AGENS BIOKONTROL JAMUR FUSARIUM OXYSPORUM F. SP. CUBENSE PENYEBAB PENYAKIT LAYU TANAMAN PISANG Anggraini, Selviana; Trisno, Jumsu; Tizelia, Tizelia
Jurnal Agroteknologi dan Pertanian (JURAGAN) Vol 1 No 1 (2020): Jurnal Agroteknologi dan Pertanian (JURAGAN) Oktober 2020
Publisher : LPPM Universitas Bina Insan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32767/juragan.v1i1.25

The use of rhizobacteria is an alternative control in suppressing plant disease attacks. The purpose of this study was to obtain indigenous rhizobacteria which have the potential as antagonist agents of Fusarium oxysporum f. sp. cubense. Sampling was carried out randomly selected on endemic lands. Isolation of rhizobacteria using serial dilution method and its antagonist test against Fusarium oxysporum f. sp. cubense uses the dual culture method. The isolation results from 3 banana cultivars (kepok, raja sere, and mas) obtained 24 rhizobacterial isolates with different morphological and physiological characters. The antagonist test results showed that 8 rhizobacterial isolates were able to suppress the growth of Fusarium oxysporum f. sp. cubense and is not plant pathogenic.

Ability of Beauveria bassiana to suppress Colletotrichum truncatum and increase the growth of chili plants (Capsicum annuum) Yanty, Dini Puspita; Trizelia, Trizelia; Darnetty, Darnetty; Trisno, Jumsu
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 1 (2024): June 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.1.42-52.2024

Anthracnose is a disease caused by Colletotrichum sp., which can reduce the quality and yield of red chili by 45-60%. This study aimed to determine the ability of different Beauveria bassiana isolates to control anthracnose in chili. The research employed a Randomized Block Design (RBD) consisting of 6 treatments and 5 replications. The treatments included a control and 5 different B. bassiana isolates (PD114, TD312, WS, BbKo, KT2B21). The observed variables included incubation period, disease incidence and severity, plant height, number of leaves, flowering time, and chili fruit weight. The results showed that the application of different B. bassiana isolates have effectively inhibited the development of Colletotrichum truncatum, which caused anthracnose in chili. B. bassiana has demonstrated the ability to prolong the incubation period of C. truncatum (5.40 days) and reduce the percentage of disease incidence (16%). Furthermore, the application of B. bassiana increased the height of chili plants (40.40 cm) and the number of leaves (32 leaves), accelerated the flowering time (42.80 days), and boosted chili production (178.25 g). The WS and TD312 isolates revealed the most significant results. Although the severity of the disease was not impacted, the 2 isolates provided notable agronomic benefits to the chili plants.

CALLI INDUCTION OF SOME CHILI PEPPER (Capsicum annuum L.) GENOTYPES AS MATERIAL FOR GENETIC TRANSFORMATION Jumsu Trisno and Jamsari, Renfiyeni, Yusniwati,
International Journal of Agricultural Sciences Vol. 1 No. 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/ijasc.1.1.75-80.2015

Calli is one of the materials used for genetic transformation. Studies on calli induction in some pepper genotypes were conducted in the Plant Tissue Culture Laboratory of the Faculty of Agriculture, Andalas University, Padang. This study aimed to obtain calli which can be used as material for genetic transformation. We used three genotypes of chili (Kopay, Kampung and Trisula). Explants were derived from first leaf, cotyledon, epycotyl and hypocotyl obtained from 24 days old chili germination. The study found that all the explants were able to produced calli. From 17 combinations of medium, the two combinations that produced 100% calli were MS + BAP + IAA + L2 vitamin and MS + 2,4 D. Mediums that can produce organogenic calli were MS + BAP 4 mg / L, IAA 0.5 mg / L, L2 vitamin ( Pyridoxin hydroklorit 0.5 g / L Â and Thiamin hydroklorit 0.1 mg / L). The colour of calli produced were yellowish green and compact. The study also found that structures develop with a globular phase, followed by a heart shape, torpedo, cotyledonary and eventually form buds. While MS + 2.4 D 4 mg / L produced yellowish white calli and crumb, it could not grow organogenic calli.

KAJIAN TEKNO EKONOMI PAKET TEKNOLOGI BAKTERI PERAKARAN PEMACU PERTUMBUHAN TANAMAN (BP3T) PUPUK KANDANG DAN NANO PESTISIDA SERAI WANGI PADA USAHA TANI KAKAO DI KABUPATEN LIMA PULUH KOTA Wahyuni, Sri; Rahma, Haliatur; Trisno, Jumsu; Martinius, Martinius; Noveriza, Rita; Reflin; Yuliani, Sri; Nusyirwan, Nusyirwan; Nefri, Jhon
Jurnal AGRISEP JURNAL AGRISEP VOL 19 NO 01 2020
Publisher : Badan Penerbitan Fakultas Pertanian, Universitas Bengkulu

Plant Growth Promoting Rhizobacteria (PGPR) plus manure technology is designed to control the vascular streak dieback disease (VSD) which attacks cocoa plants, and improve the plant growth. This research aims to compare the cost of using conventional manure and PGPR plus manure technology. This research was designed in a descriptive quantitative approach using cencus data of 43 people who use PGPR plus manure in Kabupaten Limapuluh Kota. The result shows that the conventional fertilizer costs is more expensive than the PGPR plus manure formula. Therefore, it PGPR plus manure formula is recommended for the cacao farmers in Kabupaten Limapuluh Kota.

Dual Infection of Pepper Yellow Leaf Curl Virus and Chilli Veinal Mottle Virus in Causing the Yellow leaf Curl Disease on Chili Trisno, Jumsu; Jamsari, Jamsari; Hidayat, Sri Hendrastuti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 2 (2021): December 2021
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.

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