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POTENSI RIZOBAKTERI INDIGENUS SEBAGAI AGENS BIOKONTROL JAMUR FUSARIUM OXYSPORUM F. SP. CUBENSE PENYEBAB PENYAKIT LAYU TANAMAN PISANG Anggraini, Selviana; Trisno, Jumsu; Tizelia, Tizelia
Jurnal Agroteknologi dan Pertanian (JURAGAN) Vol 1 No 1 (2020): Jurnal Agroteknologi dan Pertanian (JURAGAN) Oktober 2020
Publisher : LPPM Universitas Bina Insan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32767/juragan.v1i1.25

Abstract

The use of rhizobacteria is an alternative control in suppressing plant disease attacks. The purpose of this study was to obtain indigenous rhizobacteria which have the potential as antagonist agents of Fusarium oxysporum f. sp. cubense. Sampling was carried out randomly selected on endemic lands. Isolation of rhizobacteria using serial dilution method and its antagonist test against Fusarium oxysporum f. sp. cubense uses the dual culture method. The isolation results from 3 banana cultivars (kepok, raja sere, and mas) obtained 24 rhizobacterial isolates with different morphological and physiological characters. The antagonist test results showed that 8 rhizobacterial isolates were able to suppress the growth of Fusarium oxysporum f. sp. cubense and is not plant pathogenic.
Potensi Plant Growth-Promoting Bacteria Menekan Cucumber mosaic virus pada Tanaman Mentimun: The Potential of Plant Growth-Promoting Bacteria to Suppress Infection of Cucumber mosaic virus on Cucumber Plants Ridho, Muhammad Arif; Rahma, Haliatur; Martinius; Trisno, Jumsu
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025 - IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.38-50

Abstract

The Potential of Plant Growth-Promoting Bacteria to Suppress Infection of Cucumber mosaic virus on Cucumber Plants Mosaic disease caused by Cucumber mosaic virus (CMV) is one of the main diseases of cucumber plants, and is economically important. Plant growth-promoting bacteria (PGPB) are plant root organisms currently being developed as biological agents. This research aims to obtain the best PGPB that can suppress the development of CMV in cucumber plants. This research used a randomized block design with nine treatments and five replications. The treatments consisted of several isolates of PGPB (Serratia marsescens AR1, Alcaligenes faecalis AJ14, Stenotrophomonas pavanii KJKB 5.4, Pseudomonas fluorescens LPK1–9, Bacillus cereus AJ 34, and S. maltophilia LMTSA 5.4), orthohydroxybenzoic acid (salicylic acid), positive control, and negative control. Application of PGPB was conducted through seed treatment of cucumber seeds for 15 minutes before planting. The mosaic virus was mechanically inoculated on cucumber cotyledons seven days after planting. The variables observed were the development of mosaic disease and the growth of cucumber plants. Virus detection using specific primer pair CMV-IF/CMV-IR showed that the virus that infected cucumber plants had 98.3% similarity to the CMV isolate originating from India with accession number KJ874248.1. Pseudomonas fluorescens LPK1-9 is the best bacteria for slowing the incubation period, reducing the percentage of infected leaves, and reducing the severity of mosaic disease on cucumber plants. None of tested PGPB isolates that has the potential to increase the growth of CMV-infected cucumber plants. Therefore, further studies are needed on the effectiveness of the PGPB, such as improving how PGPB is applied.