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All Journal Pharmaciana: Jurnal Kefarmasian Naturalis : Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan PENA SAINS Jurnal Kimia Riset Journal of Health (JoH) JPM (Jurnal Pemberdayaan Masyarakat) JCES (Journal of Character Education Society) EMBRIO : Jurnal Kebidanan Jurnal Surya Masyarakat JAST : Jurnal Aplikasi Sains dan Teknologi LOYALITAS: Jurnal Pengabdian Kepada Masyarakat Health Information : Jurnal Penelitian Medicra (Journal of Medical Laboratory Science/Technology) Academia Open Madaniya Jurnal Surya Masyarakat Jurnal Pengabdian Masyarakat dalam Kesehatan Medical Scope Journal (MSJ) Jurnal Abdimas Kartika Wijayakusuma Proceedings of The ICECRS Procedia of Social Sciences and Humanities Indonesian Journal of Community Diversity and Engagement IJMI Indonesian Journal Health Science and Medicine
Jamilatur Rohmah
Universitas Muhammadiyah Sidoarjo
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Modified Mocaf Flour (Modified Cassava Flour) as Alternative Culture Media for Growth Bacteria Escherichia coli and Bacillus cereus: Modifikasi Tepung Mocaf (Modified Cassava Flour) Sebagai Media Kultur Alternatif Untuk Pertumbuhan Bakteri Escherichiacoli dan Bacillus cereus Rini, Chylen Setiyo; Saidi, Ida Agustini; Rohmah, Jamilatur
Procedia of Social Sciences and Humanities Vol. 3 (2022): Proceedings of the 1st SENARA 2022
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pssh.v3i.265

Abstract

Media is a means of growth of microorganism that contain nutrients needed by microorganisms. The high price of media and teh use of natural resources used as growth media has not been maximized, spurring researchers to find alternative media from local materials such as mocaf flour (modified cassava flour) which is a derivative product of cassava with processing techniques using the principle of fermentation by lactic acid bacteria. The purpose of thus study was to determine the number of colonies of Escherichia coli and Bacillus cereus bacteria that grew on laternative media of mocaf flour. This research is an experimental laboratory with variations in the mass of mocaf flour 1 grams, 2 grams, 4 grams, 6 grmas, 8 grams, positive controls and negative controls, the data obtained were analyzed using ANOVA test. The results showed that the average number of positive control colonies from Escherichia coli was 22.5 x 105 CFU/mL, while the positive control colonies from Bacillus subtilis were 20.25 x 105 CFU/mL. The highest average vakue of variations in the concentration of alternative media using mocaf flour (modified cassava flour) as much as 8 grams on Escherichia coli is 81 x 105 CFU/mL while Bacillus subtilis is 197.75 x 105 CFU/mL using mocaf flour (modified cassava flour) as much as 8 grams. The average value of the lowest colony number of Escherichia coli bacteria in 1 grams mocaf flour mass in 7.25 x 105 CFU/mL, for Bacillus subtilis the lowest average colony value in 2 grams mocaf flour is 27 x 105 CFU/mL. Each of treatment has a significant value of 0.05 meaning that there is no significant difference between NA medium and mocaf flour alternative media
Antibacterial Activity of Rhizome Extracts of Zingiber zerumbet (L.) Roscoeex Sm. Against Streptococcus pneumoniae: Aktivitas Antibakteri Ekstrak Rimpang Lempuyang Gajah (Zingiber zerumbet (L.) Roscoeex Sm.) Terhadap Bakteri Streptococcus pneumoniae Rohmah, Jamilatur; Rini, Chylen Setiyo
Procedia of Social Sciences and Humanities Vol. 3 (2022): Proceedings of the 1st SENARA 2022
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pssh.v3i.361

Abstract

Research on the antibacterial activity of the ethanolic extract of lempuyang (Zingiber zerumbet (L.) Roscoe ex Sm.) against Streptococcus pneumoniae has been carried out using the disc diffusion method. Streptococcus pneumoniae is a bacterium that can cause respiratory tract infections (ARI), in its handling, people use antibiotics. Antibiotics themselves if used continuously lead to resistance. Currently, there are many studies regarding traditional medicines as antibacterials. The purpose of this study was to determine the secondary metabolite content of lempuyang elephant rhizome and to determine the antibacterial activity of lempuyang extract on Streptococcus pneumoniae bacteria. The method used in this research is the diffusion method with paper discs to determine the inhibitory power. The lempuyang plants used were obtained from the village of Tegal Bulu Banyuwangi and isolates of S. pneumoniae were obtained from the Central Health Laboratory (BBLK) Surabaya. The concentrations of the extracts used were 20, 40, 60, 80, and 100% with a positive control of chloramphenicol. The results showed that the ethanol extract of lempuyang (Zingiber zerumbet (L.) Roscoe ex Sm.) could inhibit the growth of S. pneumoniae bacteria, which was known from the inhibition zone formed. The inhibition test showed that the higher the concentration of lempuyang extract, the larger the diameter of the inhibitor formed. In lempuyang, the highest inhibition diameter was found at a concentration of 100% at 43.9 ± 3.6 mm. The results of phytochemical testing showed that the ethanol extract of lempuyang (Zingiber zerumbet(L.) Roscoe ex Sm.) contained alkaloids, flavonoids, saponins, steroids, triterpenoids, phenolics, and tannins.
Penggunaan Filtrasi Sebagai Teknologi dalam Pengolahan Limbah Tahu di Desa Sepande Sidoarjo Furqonati, Lili; Fadilah, Fitrian Nur; Fitria Ayu Prayekti, Rahmatika; Kartika Putri, Aprilia; Rohmah, Jamilatur
Naturalis: Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan Vol. 13 No. 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/naturalis.13.1.32358

Abstract

There are more tofu industries in Indonesia, which means there is more waste from these sectors that need specific handling. If tofu waste with a high organic matter concentration is disposed of untreated, it might have detrimental effects on the water supply. The purpose of this study was to investigate efficient techniques for using filtration media to treat tofu waste. Combining bioball and bioring media is the primary media layout in the filtration column. The tofu industry in Sepande Village, Candi District, Sidoarjo Regency, provided the tofu waste. Each column's filtration medium has a total height of 20 cm and a 1.5-liter wastewater capacity. The results showed an improvement in the quality of tofu wastewater after treatment. Filtration media using bioballs and bioring proved effective by looking at the results of waste treatment after filtration in the form of increased pH, decreased aggressive CO2, reduced turbidity, and decreased BOD. Keywords: Tofu waste, filtrasi, bioball, bioring
Pengaruh Suhu dan Lama Penyimpanan Urine Pasien Infeksi Saluran Kemih terhadap Jumlah Bakteri Aziza, Vidayatul; Rini, Chylen S.; Aliviameita, Andika; Rohmah, Jamilatur
Medical Scope Journal Vol. 7 No. 1 (2025): Medical Scope Journal
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/msj.v7i1.55142

Abstract

Abstract:  Urinary tract infection (UTI) is caused by the growth of microorganisms in the urinary tract. The gold standard in diagnosing UTI is bacterial count. This study aimed to determine the effects of temperature and storage time for urine of patients with UTI on the number and the species of bacteria, which is important in supporting the diagnosis of UTI. This was a laboratory and experimental study using accidental sampling techniques in accordance with the inclusion criteria. The results showed that at room temperature (20-25oC) the number of bacteria increased faster and was higher than the number of bacteria stored at cold temperature (2-8oC). The number of bacterial colonies was the highest at storage time of 5 hours (5.3x104 CFU/ml) meanwhile the lowest was in fresh urine (3.4 x 104 CFU/ml). The Friedman test showed that there was an effect of temperature and storage time for urine of patients with UTI on the number of bacteria with a sig p-value of 0.001 (p<0.05). In conclusion, the average number of bacteria at room temperature (20-25oC) increased faster than at cold temperature (2-8oC) during a storage time of five hours. Keywords: urinary tract infection; number of bacteria; storage time; urine    Abstrak: Infeksi saluran kemih (ISK) diakibatkan adanya pertumbuhan mikroorganisme di dalam saluran kemih. Baku emas dalam mendiagnosis ISK ialah menghitung jumlah bakteri dalam urin. Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan lama penyimpanan urin pada pasien ISK terhadap jumlah dan jenis bakteri yang penting dalam menunjang diagnosis ISK. Penelitian ini menggunakan metode eksperimental laboratorik dengan teknik pengambilan sampel accidental sampling sesuai dengan kriteria inklusi. Hasil penelitian mendapatkan bahwa pada suhu ruang (20-25oC) jumlah bakteri meningkat lebih cepat dan lebih banyak dibandingkan jumlah bakteri pada suhu dingin (2-8oC). Rerata jumlah koloni bakteri yang tertinggi yaitu sebesar 5,3x104 CFU/ml didapatkan pada waktu penyimpanan 5 jam di suhu ruang, dan yang terendah pada urin segar sebesar 3,4 x 104 CFU/ml. Hasil uji Friedman menunjukkan terdapat pengaruh suhu dan lama penyimpanan urin pada pasien ISK terhadap jumlah bakteri dengan nilai p=0,001 (p<0,05). Simpulan penelitian ini ialah rerata jumlah bakteri pada suhu ruang (20-25oC) meningkat lebih cepat dibandingkan pada suhu dingin (2-8oC) selama waktu penyimpanan 5 jam. Kata kunci: infeksi saluran kemih; jumlah bakteri; lama penyimpanan; urin
High Dose Sesbania Grandiflora Extract Alters Rat Kidney Electrolytes based on Insights from Indonesia Hidayat, Mokhamad Amirul; Rohmah, Jamilatur
Academia Open Vol 9 No 2 (2024): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/acopen.9.2024.7812

Abstract

This study investigates the acute toxicity of white turi flower extract (Sesbania grandiflora) on kidney electrolyte levels in rats (Rattus norvegicus). Conducted at Muhammadiyah University of Sidoarjo, male Wistar rats were divided into control and treatment groups, receiving 10,000, 15,000, and 20,000 mg/kgBW doses. Symptoms of toxicity included lethargy and hair loss, but no deaths occurred. Despite normal macroscopic kidney observations, sodium and potassium levels significantly increased with higher doses (p=0.000). These findings suggest that high doses of white turi flower extract can affect kidney electrolytes, indicating potential toxicity. Further research is needed to assess long-term safety for medicinal use. Highlights: 1. High doses increase sodium and potassium in rat kidneys.2. Symptoms: lethargy, hair loss, no fatalities.3. Requires further research for long-term safety. Keywords: Sesbania grandiflora, acute toxicity, kidney electrolytes, sodium, potassium
Specification Acupuncture Points Li4 and Sp6 in Postpartum Sectio Caesarea Pain Nurul Azizah; Rafhani Rosyidah; Jamilatur Rohmah
EMBRIO: Jurnal Kebidanan Vol 15 No 2 (2023): EMBRIO: Jurnal Kebidanan (NOVEMBER)
Publisher : Program Studi S1 Kebidanan - Fakultas Ilmu Kesehatan Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/embrio.v15i2.7281

Abstract

Postoperative pain can affect the postpartum SC's mobility, and the physical healing process after surgery can also trigger physiological stress and anxiety due to wound pain. The presence of side effects from analgesic use prompts the exploration of non-pharmacological treatments as an alternative for pain management in post-SC clients, as they are considered to have low side effects. Acupuncture, as one of the non-pharmacological complementary therapies, has been developed to address pain. This research aims to identify the Specific Points of Acupuncture, Li4 and Sp6, in Postpartum Cesarean Section (SC) Pain. The research design employed a quasi-experimental clinical approach, specifically a non-equivalent pre-posttest with a control group. This involved identifying pain reduction before and 24 hours after administering acupuncture intervention at specific points, LI4 and SP6, for a duration of 30 minutes. The study included a total of 34 participants in both the treatment and control groups. The research was conducted at Airlangga University Hospital in Surabaya. The subjects of the study were patients who met the inclusion criteria. The Pain Numeric Rating Scale was used as the research instrument. Statistical analysis involved employing one-way ANOVA to observe the mean change in values between the pre-test and post-test within each group. The administration of acupuncture therapy targeting points LI4 and SP6 in this study proved effective in reducing postpartum SC pain compared to the control group. In future research, further investigation could be conducted to identify changes in chemical reactions within the body following acupuncture treatment.
ANALYSIS OF DIFFERENCES IN TRIGLYCERIDE LEVELS BETWEEN TOBACCO SMOKERS AND ELECTRIC SMOKERS IN STUDENTS (ORMAWA) OF MUHAMMADIYAH UNIVERSITY OF SIDOARJO Mukarromah, Rizka Aisya; Hanum, Galuh Ratmana; Rohmah, Jamilatur; Rini , Chylen Setiyo
International Journal Multidisciplinary (IJMI) Vol. 2 No. 4 (2025): International Journal Multidisciplinary (IJMI)
Publisher : ANTIS PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/ijmi.v2i4.328

Abstract

Objective: The aim of this study was to analyze the differences in triglyceride levels between tobacco smokers and e-smokers among students (ORMAWA) at Muhammadiyah University of Sidoarjo. Method: The design used in this research was quantitative with experimental tests using a cross-sectional approach. The population of this study included all male Ormawa students at Muhammadiyah University of Sidoarjo with the habit of smoking tobacco and e-smoking, totaling 532 students, with a sample size of 16 tobacco smokers and 16 e-smokers. The sampling method used is Quota non-probability sampling. This research was conducted at the hospital. Bhayangkara Pusdik Sabhara Porong in August 2024. The samples in the study were read using a DIALAB brand autolyzer with the colorimetric enzymatic method (GPO-PAP). Data from examination of triglyceride levels were analyzed statistically using the t-Independent test. Results: The results showed that there was no significant difference, namely 0.055 (p<0.05) between tobacco smokers and electronic smokers among students (Ormawa) at Muhammadiyah University of Sidoarjo. Novelty: This study provides comparative data on triglyceride levels between tobacco and electronic cigarette users within a specific university student organization population, which has not been widely studied in the Indonesian context.
Peningkatan Keterampilan Siswa Pada Pemeriksaan Mikrobiologi Air Minum Di SMK Muhammadiyah 1 Pandaan Rini, Chylen Setiyo; Mushlih, Miftahul; Efendi, Nur; Rohmah, Jamilatur
Jurnal Abdimas Kartika Wijayakusuma Vol 6 No 2 (2025): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v6i2.876

Abstract

Air sangat penting bagi kelangsungan hidup manusia, karena manusia sebagai makhluk hidup sangat membutuhkannya. Air minum adalah air yang sudah mengalami atau tidak mengalami proses pengolahan, namun tetap memenuhi standar kesehatan dan aman untuk langsung dikonsumsi. Salah satu syarat kriteria yang untuk memenuhi standar air minum yang berkualitas yakni kriteria biologis yang terbebas dari kontaminasi bakteri, terutama yang bersifat patogen. Pemeriksaan mikrobiologi belum diajarkan pada sekolah SMK Muhammadiyah 1 Pandaan. Melihat kondisi tersebut, tim pengabdi masyarakat TLM UMSIDA bekerja sama dengan SMK Muhammadiyah 1 Pandaan memberikan pelatihan pemeriksaan mikrobiologi dari sampel air minum. Kegiatan pengabdian kepada masyarakat dilakukan pada Juli-Agustus 2024 yang diikuti 53 siswa/i.. Hasil analisis dari kegiatan ini yaitu meningkatnya pemahaman dari siswa/i mengenai pemeriksaan mikrobiologi dari sampel air minum.
Optimasi Kecepatan Sentrifugasi Sampel Darah untuk Pemeriksaan Diabetes Melitus Tipe 2 (T2D) Berbasis Molekuler Miftahul Mushlih; Fitrian Desi Prameswari; Jamilatur Rohmah; Andika Aliviameita; Mushlih, Miftahul
Journal of Health Vol. 12 No. 2 (2025): Journal of Health (JoH) - July
Publisher : LPPM STIKES Guna Bangsa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v12n2.5

Abstract

Type 2 Diabetes Mellitus (T2D) is a metabolic disorder caused by dysfunction of insulin secretion by pancreatic beta cells and the inability of insulin tissue to respond appropriately to insulin. Molecular-based examination can make it easier to determine appropriate diagnostic biomarkers and the biology of this disease appears long before clinical symptoms develop. Blood isolation samples from the buffy coat contained higher levels of DNA than whole blood samples. The aim of this research is to determine whether there are differences in the results of the centrifugation speed of blood samples for molecular-based examination of Type 2 Diabetes Mellitus (T2D). Optimal centrifugation speed can produce high-quality DNA required for molecular analysis, thereby improving the accuracy of diagnosis and effectiveness of T2D treatment. DNA contaminated by cellular debris can result in small amounts of DNA obtained for further analysis. The research was carried out qualitatively using descriptive experimental research using purposive sampling techniques. The total samples used were 25 samples, taken from 5 patients and then divided into 5 treatment groups (no centrifugation, centrifugation speed 500 rpm, 1500 rpm, 3000 rpm and 4500 rpm for 5 minutes). Test testing is carried out qualitatively (electrophoresis). The results showed that there was no significant difference in DNA visualization in molecular examination of T2D disease between blood samples that were not centrifuged and those that were centrifuged at speeds of 500 rpm, 1500 rpm, 3000 rpm and 4500 rpm for 5 minutes. However, centrifugation speeds between 1500 rpm and 4500 rpm showed thicker and clearer DNA bands.
Toxicity of White Turi Leaf Extract on Liver Function in Mice: Toksisitas Ekstrak Daun Turi Putih pada Fungsi Hati Mencit Makrufah, Makrufah; Rohmah, Jamilatur
Indonesian Journal on Health Science and Medicine Vol. 2 No. 2 (2025): Oktober
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v2i2.246

Abstract

General background: Sesbania grandiflora (L.) Pers., widely known as white turi, is traditionally used as analgesic, laxative, and other medicinal purposes. Specific background: Despite its therapeutic benefits, the plant contains secondary metabolites, including triterpenoids and alkaloids, with potential hepatotoxic properties, particularly pyrrolizidine alkaloids. Knowledge gap: Previous studies have indicated general toxicity of turi extracts but limited evidence exists on its subacute hepatic toxicity measured through enzymatic markers in animal models. Aim: This study investigated the toxicity of white turi leaf extract on liver function by assessing SGOT and SGPT levels in mice (Mus musculus). Results: Laboratory experiments using a posttest-only control group design were conducted with multiple dosage levels. Statistical analysis with Kruskal-Wallis revealed no significant differences in SGOT (p = 0.896) and SGPT (p = 0.527) levels between treatment and control groups, while ANOVA showed no significant change in liver weight (p = 0.089). Novelty: This research provides the first evidence that ethanolic white turi leaf extract, within the tested doses and 14-day observation, does not induce measurable hepatic dysfunction in mice. Implications: The findings suggest relative safety of white turi extract at tested doses, supporting its potential traditional use while highlighting the importance of further chronic toxicity studies. Highlights : White turi leaf extract showed no hepatotoxic effect in mice. SGOT-SGPT levels and liver weight remained within normal range. Supports safe traditional use but requires chronic toxicity evaluation. Keywords : Sesbania Grandiflora, White Turi Leaf, SGOT, SGPT, Hepatotoxicity
Co-Authors Achmad Noerkhaerin Putra Aini, Latifatul Andika Aliviameita Anwar, Najih Arini Siti Wahyuningsih Aziza, Vidayatul Chylen Setiyo Rini Devi Arvindani Masyitha Eni Fariyatul Fahyuni Fadilah, Fitrian Nur FATMAWATI, RIRIN Firdaus, Firyalita Sarah Fitria Ayu Prayekti, Rahmatika Fitria Eka Wulandari Fitrian Desi Prameswari Fiya, Uus Ufita Furqonati, Lili Galuh Ratmana Hanum Handini, Hesti Dwi Hidayat, Mokhamad Amirul Ida Agustini Saidi Karlia Hedit Tiana Khusniyah, Nurul Liyaajul, Pratasyah Makrufah, Makrufah Margareta, Frida Amelia Marlina, Mariany Lambertine Martina, Shofie Fitria Miftahul Mushlih Miranda, Niken Anggita Mukarromah, Rizka Aisya Novitasari, Fia NUR EFENDI Nurul Azizah NURUL AZIZAH Paramitha Amelia Kusumawardani Purwanti, Yanik Purwanti Puspitasari Puspitasari Putra, Moh. Faris Yudiana Rafhani Rosyidah Rini , Chylen Setiyo Rini, Chylen S. Rofidah, Luthfiyah Salsabila Widya Kirana Siroj, Muhammad Said Agil Siti Cholifah SITI CHOLIFAH Sofi Rahayu, Bella Tri Chahyani Rahmawati Putri Tri Linggo Wati Vanda Rezania Wakhidah, Kemil Widyaningrum, Leni Yuroh Yuli Astutik Zulfida Ainindiyah Putri Purwanto Zurrotul Ilmiyah