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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Agrointek Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan JURNAL PENGABDIAN KEPADA MASYARAKAT Acta Aquatica: Aquatic Sciences Journal Jurnal Pengabdian Pada Masyarakat MATAPPA: Jurnal Pengabdian Kepada Masyarakat Jurnal Aplikasi Teknologi Pangan Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ilmu Kefarmasian Indonesia Sarwahita : Jurnal Pengabdian Kepada Masyarakat Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Rural and Urban Community Empowerment JURNAL AGROINDUSTRI HALAL Teknologi dan Industri Pertanian Indonesia Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Jurnal KALANDRA Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Andarini Diharmi
Departemen Teknilogi Hasil Perikanan Fakultas Perikanan Dan Kelautan Universitas Riau
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Agrointek

 1 
Pengaruh suhu berbeda terhadap aktifitas enzim kolagenase dari usus ikan cunang (Congresox talabon) Edison Edison; Andarini Diharmi; Mirna Ilza; Rahman Karnila; Febrina Tumangger
AGROINTEK Vol 18, No 1 (2024)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v18i1.17548

Abstract

Collagenase enzyme is a type of protease enzyme. The activity of an enzyme is affected by substrate, temperature, inhibitor, and pH.  One of the sources of enzymes is the internal organs of fish, namely the gut. This study aims to determine the activity of the collagenase enzyme at different temperatures. The experimental design used was randomized and full non-factorial. The treatments used were different incubation temperatures (50, 55, and 60oC) with three replications. Parameter analysis comprised fish proportion, enzyme activity, and dissolved protein. The results showed that the parts of the yellow pike conger fish were meat (64.25%), bones (19.98%), internal organs (including intestines) (4.50%), and skin (6.30%). Collagenase enzyme activity at temperatures 50, 55, and 60oC was 1.154, 1.702, and 0.667 U/mL, respectively. The dissolved proteins content was 12.89 mg/mL. The optimum temperature of the collagenase enzyme from the fish gut was 55oC with an enzyme activity of 1,702 U/mL. The dissolved protein content of the gut yellow pike conger extract was 12.89 mg/mL  Purification of the collagenase enzyme from of the gut yellow pike conger was needed to obtain a higher enzyme activity value.
Identifikasi komponen bioaktif dan aktivitas antibakeri rumput laut cokelat (Sargassum plagyophyllum) terhadap bakteri Staphylococcus aureus dan Escherichia coli Noor Ira Sari; Indah Azisari; Andarini Diharmi
AGROINTEK Vol 18, No 1 (2024)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v18i1.13352

Abstract

Sargassum plagyophyllum is a type of brown seaweed containing bioactive components including alkaloids, flavonoids, steroids/terpenoids, saponins and phenolics that have potential as antibacterial. This study aims to determine the bioactive components of the S. plagyophyllum extract fraction qualitatively and the antibacterial activity of the S. plagyophyllum fraction by agar diffusion method with wells. The method used in this research is an experiment by extracting and fractionating S. plagyophyllum. Parameter analysis consisted of analysis of bioactive components (alkaloids, flavonoids, steroids/terpenoids, saponins, phenolics) qualitatively and determining antibacterial activity in the S. plagyophyllum fraction against Staphylococcus aureus and Escherichia coli. The results showed that the bioactive components in the methanol extract of S. plagyophyllum contained flavonoids, steroids/terpenoids, saponins and phenolic compounds. The bioactive components in the ethyl acetate fraction contained alkaloids, flavonoids, steroids/terpenoids, saponins and phenolics. The n-hexane and butanol fractions contained steroids/terpenoids, and saponins. The extract fraction of S. plagyophyllum could inhibit the growth of antibacterial activity against S. aureus and E. coli bacteria. The ethyl acetate fraction was the best fraction inhibiting bacterial activity with the highest zone of inhibition compared to other fractions (n-Hexane and buthanol), namely 12.95 ± 0.64 mm for S. aureus bacteria and 10 ± 0.14 mm for E. coli bacteria.
Aktivitas antioksidan, total fenolik, flavonoid dan saponin anggur laut (Caulerpa lentillifera) diekstrak dengan pelarut yang berbeda polaritas Andarini Diharmi; Edison Edison; Mirna Ilza; Dahlia Dahlia; Reza Saputra
AGROINTEK Vol 18, No 3 (2024)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v18i3.12240

Abstract

Caulerpa lentillfera is a species of seaweed that belongs to the class Chlorophyceae (green seaweed). Caulerpa lentillifera contains secondary metabolites consisting of alkaloids, flavonoids, steroids, terpenoids, saponins and phenols that can be used as antioxidants, antimicrobials, anticancer and others.  This study was aimed to determine the total phenolic flavonoid compounds, saponins, and antioxidant activity of Caulerpa lentillifera extract. The research method used is an experiment by conducting a series of experiments, namely the extraction of Caulerpa lentillifera using the maceration method in stages with solvents of different polarity. The solvents used consisted of non-polar (n-hexane), semi-polar (ethyl acetate) and polar (methanol).  The analysis parameters consisted of yield analysis, total phenol, flavonoid, saponin, and antioxidant activity.  The yield of Caulerpa lentillifera extract extracted with n-hexane, ethyl acetate, and methanol was 0.36; 0.39; and 0.42%.  The content of saponins produced from hexane, ethyl acetate and methanol extracts were 0.14%; 0.17%; and 0.20%, respectively, in a 100 g sample.  The total phenol produced in the ethyl acetate and methanol extracts were 141,50 mgGAE/100 g and 154,65 mg GAE/100 g, respectively.  Flavonoid content was 116,82 mg QE/100 g only found in methanol extract.  The antioxidant activity of n-hexane, ethyl acetate, and methanol extracts with IC50 values were 234.28, 203.96, and 124,77 ppm, respectively.  Extracts of Caulerpa lentillifera with different polarity solvents were produced different bioactive components. Saponins were produced in all three types of solvents, phenol  were found in ethyl acetate and methanol extracts and flavonoids only in methanol extract. The highest antioxidant activity of Caulerpa lentillifera was produced with methanol extract with IC 50 value of 124,77 ppm.
Co-Authors Agus Syahroni Agusman, Indra Akhyar Ali Alghifary, Zaki Allecya Tri Elisabeth Sihombing Anggi Angelita Hermay Anggi Angelita Hermaya Azmi, Heriza Br. Sianturi1, Frisdauli junitaMargaret Chicka Willy Yanti Dahlia Dahlia Deando Hentun Gultom Dedi Fardiaz Deviasari Deviasari Dewi Fortuna Ayu Dewita Dewita Dewita, Dewitad Dian Iriani Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Ela Davera Sari Emma Susanti Enda Mora Endang Sri Heruwati Endang Sri Heruwati Fachrul Roji Fatma Eka Putri Febriani Melisa Sinurat Febriani Melisa Sinurat Febrina Tumangger Febrina Tumangger Feby Dwi Anisha Putri Helmi Nurhanif Henni Syawal Herawati Br Ginting Heriyanti Heriyanti, Heriyanti Hermaya, Anggi Angelita Hotmeriah Padang Illahi, Sikarina Ilman Arif Indah Azisari Indah Azisari Indra Agusman Indra Agusman Ira Sari Irasari, N. Irene Charistia Jhosua Fransiskus Karnila, Rahman Karnila Kenzo Aditya Muhammad Yandhria Putra Kristina Natalia Kusai Kusai Kusai, Kusai Lamun Bathara Lorde Sembiring Lorde Sembiring M Lamudin Noor M. Faiz Akhimuddin M. Ufan M. Ufan M.Faisal Saputra S M.Halim M.Halim Manurung, Rahel Eriani Maria Marifa Indar Wara Gandini Mery Sukmiwati Mirna Ilza Mirna Ilza Mirna Ilza Muhammad Arief Muhammad Zakiyul Fikri N Ira Sari N Ira Sari N Ira Sari N Ira Sari N Ira Sari N. Ira Sari N. Nurjanah ndang Sri Heruwati Nofri Sandria Nola Rahma Aulia Noor Ira Sari Nur Ira Sari Nuraini Nuraini Nurhanif, Helmi Nuri Andarwulan Nurul Muji Ariani Oktavianti, Mila Panji Yaversio Praja Prayogo Priady Simatupang Putra, Sanjaya Mandala Putri Rahmadani Putri, Fatma Eka Putri, Indah Prasetya PUTRIANA SARI SIRAIT Rana Salma Reza Saputra Reza Saputra Rezky Fransiskus Simanjuntak Rindi Metalisa, Rindi Rossi, Evy RR. Ella Evrita Hestiandari Rusnawati Rusnawati Salma, Putri Santhy Wisuda Sidauruk Santika, Jesi Sari, Noor Ira Selamat ariyadin Sidauruk, Santhy W. Silaban, Deni Saputra Silfi Zuhriani Siregar, Cici YP Siregar, Ika Darmila Siregar, Rizky Febriansyah Siti Aminah Siti Khaizatul A’mal Suardi Loekman Sukendi Sukendi Sulistiana Pramita Nosa Sumarto Sumarto Sumarto Suparmi Suparmi Suparmi Syahada, Melania Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Tessha Fitriani Tiara Kurnia Sari Tiara Septiani Lumban Gaol Tince Sofyani Tince Sofyani Titieu Keumala Sukandar Tomi Ramadona Tri Ayu Ningsih Trisla Warningsih Ulil Amri Wahyudi Wahyudi Wikky Aditiya Putra Yenita Roza Yuni Afrida Yuni Suseno