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All Journal AL KAUNIYAH Quagga BIO-SITE |BIOLOGI Sains Terapan BIOMA DIDAKTIKA BIOLOGI: Jurnal Penelitian Pendidikan Biologi Jurnal Biodjati Wacana Akademika : Majalah Ilmiah Kependidikan BIOEDUSCIENCE Jurnal SOLMA Assimilation: Indonesian Journal of Biology Education JPBIO (Jurnal Pendidikan Biologi) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Biomedika Journal of Community Service and Empowerment Biodik: Jurnal Ilmiah Pendidikan Biologi Journal of Biotropical Research and Nature Technology
Susilo Susilo
Universitas Muhammadiyah Prof. Dr. Hamka
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Pengaruh Strategi Predict Observe Explain (POE) Terhadap Kemampuan Berpikir Kritis Siswa: The Impact of Predict Observe Explain (POE) strategy against student's critical thinking ability Gufron Amirullah; Rizkia Suciati; Susilo Susilo; Dewi Handayani
BIODIK Vol. 5 No. 2 (2019): September 2019
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/bio.v5i2.7165

Abstract

Learning strategy is a very decisive factor in student learning outcomes. This study wanted to know the influence of Predict Observe Explain (POE) strategy to the critical thinking ability of grade X students in environmental pollution material at SMA Negeri 3 Bekasi. The study used Pretest-Posttest Control Group Design with a sampling technique of Cluster Random Sampling (N: 90). Research instruments in the form of 10 test descriptions that have been integrated with the ability of critical thinking are used for data collection. The research Data is analyzed using the normality test, homogeneity test, and T test. Our research results show the average value of the experiment class (x = 75.03) greater than the control class (x = 65.57). The hypothesis Test (T-Test) with a signification level of 1% obtained a Thit value of 5.25 & this 2.39. Our research suggests that POE learning strategies can affect students ' critical thinking skills. This POE strategy can be used as a referral for biological teachers to improve the students ' critical thinking skills. Keywords: POE strategy, essential thinking ability, environmental pollution. Abstrak. Strategi pembelajaran merupakan faktor yang sangat menentukan hasil belajar siswa. Studi ini ingin mengetahui pengaruh strategi Predict Observe Explain (POE) terhadap kemampuan berpikir kritis siswa kelas X pada materi pencemaran lingkungan di SMA Negeri 3 Bekasi. Penelitian ini menggunakan Pretest-Posttest Control Group Design dengan teknik sampling Cluster Random Sampling (n: 90). Instrumen penelitian berupa 10 soal tes uraian yang telah terintegrasi dengan kemampuan berpikir kritis digunakan untuk pengumpulan data. Data penelitian dianalisis dengan menggunakan uji normalitas, uji homogenitas, dan uji t. Hasil penelitian kami menunjukkan nilai rata-rata kelas eksperimen (x = 75,03) lebih besar dari kelas kontrol (x = 65,57). Uji hipotesis (uji t) dengan taraf signifikasi 1 % diperoleh nilai thit sebesar 5,25 & ttabel 2,39. Penelitian kami menunjukkan bahwa strategi pembelajaran POE dapat mempengaruhi kemampuan berpikir kritis siswa. Strategi POE ini dapat dijadikan rujukan guru biologi untuk meningkatkan kemampuan berpikir kritia pada siswa. Kata Kunci : Strategi POE, Pencemaran Lingkungan, Kemampuan Berpikir Kritis.
Analisis Keterampilan Proses Sains Calon Guru Biologi Terhadap HOTS: Analysis of Pre-service Teachers Biology Science Process Skills towards HOTS Lutphi Safahi; Arum Pusporini; Susilo Susilo; Budhi Akbar
BIODIK Vol. 6 No. 1 (2020): March 2020
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/bio.v6i1.8565

Abstract

The low problem-solving ability in understanding biology lessons makes biology lessons difficult for some prospective teachers to learn. This study aims to determine the contribution of science process skills to higher-order thinking skills. This study uses quantitative methods to analyse the HOTS skills of prospective biology teachers. The quantitative descriptive method used in this study. Data collected from 59 respondents through the Saturated Sampling technique. The instrument used was 54 multiple choice questions with indicators of science process skills and higher-order thinking skills. The data obtained is processed quantitatively with Microsoft Excel. Prospective Biology teachers have the contribution of science process skills with a high level of ability of 13% and a contribution is known. This research proves that the contribution of science process skills to prospective biology teachers to higher-level thinking skills has low categorisation. Keywords: Science Process Skills; Pre-service Teachers Biology; High-level Thinking Ability; biology lessons. Abstrak. Rendahnya kemampuan pemecahan masalah dalam memahami pelajaran biologi menyebabkan pelajaran biologi menjadi sulit dipelajari oleh sebagian calon guru. Studi ini bertujuan untuk mengetahui kontribusi keterampilan proses sains terhadap kemampuan berpikir tingkat tinggi. Penelitian ini menggunakan metode kuantitatif untuk menganalisa keterampilan HOTS calon guru biologi. Metode penelitian yang digunakan adalah diskriptif kuantitatif. Data dikumpulkan dengan sampel sebanyak 59 mahasiswa melalui teknik Sampling Jenuh. Instrumen yang digunakan berupa 54 soal pilihan ganda dengan indikator keterampilan proses sains dan kemampuan berpikir tingkat tinggi. Data yang diperoleh diolah secara kuantitatif dengan Microsoft Excel. Calon guru Biologi memiliki hasil kontribusi keterampilan proses sains dengan kemampuan berpikir tingkat tinggi sebesar 13% dan diketahui adanya kontribusi. Penelitian ini membuktikan bahwa kontribusi keterampilan proses sains calon guru biologi terhadap kemampuan berpikir tingkat tinggi memiliki kategori rendah. Kata kunci: Keterampilan Proses Sains; Calon guru biologi; HOTS; Pelajaran biologi.
Evaluation of ISSR Primer Applicability for Interspecific Differentiation of Marchantia from West Java Susilo Susilo; Maryanti Setyaningsih; Suci Lestari; Jahanzaib Ali
Jurnal Biodjati Vol 11 No 1 (2026): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v11i1.54766

Abstract

Liverworts of the genus Marchantia play important ecological roles in humid tropical ecosystems; however, molecular marker evaluation for tropical taxa remains limited. This study aimed to assess the amplification performance and cross-species applicability of selected Inter Simple Sequence Repeat (ISSR) primers for interspecific molecular differentiation of Marchantia species from West Java, Indonesia. Four species (M. emarginata, M. geminata, M. paleacea, and M. polymorpha) were analyzed using five ISSR primers. Genomic DNA was extracted using a modified CTAB protocol and amplified through single-primer ISSR-PCR assays. Amplification profiles were scored as the presence or absence of dominant bands. Three primers (F218, F231, and F209) generated clear and reproducible multi-locus banding patterns, producing a total of 21 scorable fragments, whereas BS537 and BS3 failed to amplify detectable products. Polymorphism Information Content (PIC), interpreted as an index of band discrimination capacity across species, ranged from 0.70 to 0.91, with F209 and F218 showing the highest informativeness. The results demonstrate that the selected ISSR primers enable reliable interspecific discrimination among the taxa examined and provide a practical framework for preliminary molecular screening of tropical bryophytes. These findings support the use of validated ISSR primers in future taxonomic and comparative genetic studies of non-model liverwort species.
Linking Untargeted Metabolomics to Functional Bioactivity in Nutmeg (Myristica fragrans Houtt.): Current Advances, Bioactivity Integration, and Analytical Challenges Susilo Susilo; Ratna Yuniarti; Titin Siswantining; Yasman Yasman
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.449

Abstract

Myristica fragrans Houtt. has been widely explored due to its high chemical composition and many functional features, nevertheless, the analytical framework applied to analyze this species has progressed unevenly. This study critically discusses the shift from conventional GC–MS-based phytochemical profiling to more integrated metabolomics techniques incorporating LC-HRMS, chemometrics, and bioactivity analysis in nutmeg research. A literature study was undertaken in five scientific databases using PRISMA guidance. Studies were categorized as MAIN and SUPPORT to distinguish between metabolomics-driven studies and studies providing complementary evidence of phytochemical and bioactivity data. The synthesis illustrates that most of the published work still depends on GC–MS analysis of volatile fractions and essential oils, but fully untargeted metabolomics investigations are rare. Recent developments have increased metabolite coverage, sample discrimination and helped identification of potential markers related to bioactivity interpretation, species authentication and quality assessment. Most studies still tend to report relationships between metabolites and bioactivity mostly as compositional or statistical associations, biomarker verification remains patchy across the literature and metabolite annotation is still often limited to MSI Level 2 or 3, although examples of higher-confidence identification are emerging. In sum, contemporary M. fragrans research can best be characterized as a shift from extended phytochemical profiling to more biologically interpretable metabolomics. Future advances will depend on increased standardization, more confident metabolite annotation, stricter chemometric validation, and wider integration of chemical signatures with functional biological evidence to enable more robust and translatable applications of nutmeg metabolomics.
Pengaruh Ekstrak Etanol Daun Sambiloto Terhadap Jumlah dan Motilitas Spermatozoa Mencit Jantan Susilo Susilo; Budhi Akbar; Ika Pratinaningsih
Jurnal Biodjati Vol 3 No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3505

Abstract

Tanaman sambiloto (Andrographis paniculata Ness.) di Indonesia dikenal sebagai salah satu tanaman yang berkhasiat sebagai obat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun sambiloto terhadap jumlah dan motilitas spermaozoa mencit (Mus musculus L.) jantan galur DDY. Sebanyak 24 ekor mencit jantan dengan berat antara 100-125 g yang dibagi menjadi empat kelompok perlakuan, diberikan ekstrak etanol secara oral selama 15 hari. Kelompok mencit pada perlakuan satu (P1) diberikan ekstrak etanol daun sambiloto 84 mg/kg bb. Kelompok mencit pada perlakuan dua (P2) dan tiga (P3) masing-masing diberikan ekstrak etanol daun sambiloto 140 mg/kg bb dan 196 mg/kg bb. Sedangkan kelompok kontrol (P0) tidak diberi perlakuan. Hasil penelitian menunjukkan bahwa pada kelompok perlakuan P3 (196 mg/kg bb) menunjukkan jumlah spermatozoa terbanyak yaitu 7,33 juta/ml, mengalami penurunan sebesar 78,86% terhadap kontrol.  Kandungan senyawa andrografolid dan flavonoid pada ekstrak sambiloto diduga kuat menjadi penyebab penurunan jumlah dan motilitas spermatozoa mencit. Hasil penelitian ini memberikan informasi bahwa pemberian ekstrak etanol daun sambiloto berpengaruh terhadap jumlah dan motilitas spermatozoa mencit jantan.
Co-Authors Agung Purwanto Agus Pambudi Darma Ahmad Ali Amirullah, Gufron Arum Pusporini Budhi Akbar Budhi Akbar Budhi Akbar Budhi Akbar, Budhi Daniel Fernandez Desya Aryani Sofyan Devi Anugrah Devi Anugrah Devi Anugrah Dewi Handayani Diana Vivanti Sigit Diana Vivanti Sigit Dwi Meilina Andrianni Edi Kurniawan Giry Marhento Gustomi Gustomi Gustomi, Gustomi Hayati Amali Henita Rahmayanti Hilman Faruq Ika Pratinaningsih Ilena Amalia Luthfi Ilmi Zajuli Ichsan Irwandani Jahanzaib Ali Liza Verizza Triansyah Lutfah Nurusman Lutphi Safahi Maryanti Setyaningsih Maryanti Setyaningsih Md. Mehadi Rahman Meitiyani Meitiyani Merina Merina Muhammad Efendi Muharomah, Dewi Robiatun Murwitaningsih, Susanti Mushoddik, M. Nurusman, Lutfah Ratna Yuniarti Rizhal Hendi Ristanto Rizkia Suciati Ruqiah Ganda Putri Panjaitan Safahi, Luthpi Suci Lestari Sulastri Sulastri Susanti Murwitaningsih Syintia, Syintia Titin Siswantining Wibisono, Danu Yarza, Husnin Nahry Yasman Yasman Yassir Dzulfiqor Yeni Widiyawati