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POTENSI ISOLAT MIKROBIA ASAL ANDISOL DIENG, JAWA TENGAH SEBAGAI INOKULUM PUPUK HAYATI PENGOKSIDASI SULFUR Sudadi Sudadi; Ika Ernawati; Sumarno Sumarno; Widyatmani Sih Dewi; Hery Widijanto
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 10, No 1 (2013)
Publisher : Faculty of Agriculture, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v10i1.130

Abstract

Title : Study on The Potency of Soil Microbes Isolated from Andisols of Dieng, Central Java,  as S-Oxidizing Biofertilizer Inoculant. The problem of nutrient deficiences tend to increase, including sulfur deficiency. The use of high purity NPK fertilizers stimulate sulfur deficiency in agricultural land. This problem can be corrected by the use of elemental sulfur accompanied with S-oxidizing biofertilizer. The study aimed to evaluate the potential of sulfur oxidizing microbial isolated from Andisols of Dieng Central Java as S-oxidizing inoculant. Andisols Dieng naturally contains high sulfur that allegedly inhabited many sulfur-oxidizing microbials. The research consisted of two experiments, each arranged in completely randomized design (CRD). Each experiment consist of two factors namely the kind of microbial isolated from Andisols Dieng and incubation time. The first experiment used Czapek-dox+elemental sulfur medium, while the second experiment used vermicompost as medium. The observed variables include soluble-S, bacterial density, biomass of fungi and medium pH. One isolate of bacteria (I1)and three isolates of fungi (F1, F2 and F3) taken from Andisol of Dieng showing their ability to oxidize sulfur in Czapek-dok agar medium. They were used as isolates in the experiment. The result of the first experiment showed that the oxidized sulfur increased with the length of incubation time as indicated by the increase of soluble-S, bacterial cell density or fungi biomass in the medium followed by the decrease of pH. The second experiment showed that the population of bacteria or fungi isolates and soluble-S of vermicompost were increased collaterally with incubation time. The fungi isolate of F2 have the highest potency as inoculant of S-oxidizing biofertilizer as shown by it ability to survive, grows and oxidize S both in Czapek-dox+elemental sulfur liquid medium as well as in vermicompost medium.
Improvement Soil Biology Characteristics of Paddy Field by System of Rice Intensification Widyatmani Sih Dewi
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 12, No 2 (2015)
Publisher : Faculty of Agriculture, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v12i2.475

Abstract

The aim of the research was to test the System of Rice Intensification (SRI) method in improving the biological properties of paddy soil. The indicators of improvement were measured by the number of earthworm feces (cast), and the population of some microbial and nutrient content in the cast. The experiments were performed by comparing the three methods, namely: (1) SRI, (2) semi-conventional, and (3) conventional, using Randomized Completely Block Design. Each treatment was repeated nine times. The experiments were performed in the paddy fields belonging to farmers in Sukoharjo, Central Java. The result showed that the SRI (application of 1 tons ha-1 of vermicompost + 50% of inorganic fertilizer dosage) tends to increase the number of earthworms cast. It is an indicator of earthworm activity in soil. Earthworms cast contains more phosphate solubilizing  bacteria (12.98 x 1010cfu) and N content (1.23%) compared to its surrounding soil. There is a close functional relation between earthworms cast with total tiller number. SRI method is better than the other two methods to improve the biological characteristics of paddy soil that has the potential to maintain the sustainability of soil productivity.
The Study of Artemisinin Content in Callus Artemisia annua L. Cultures Elicited with Endophytic Fungi Aspergillus sp. Farida Yuliani; Widyatmani Sih Dewi; Ahmad Yunus; Usman Siswanto
Molekul Vol 13, No 2 (2018)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.067 KB) | DOI: 10.20884/1.jm.2018.13.2.459

Abstract

Artemisinin, is a sesquiterpene lactone which is originally extracted from the medicinal plant Artemisia annua L. Artemisinin is a very effective antimalarial agent, however it is produced in very low amounts in plants. Chemical synthesis of artemisinin is complicated and not economically feasible. Researchers often use elicitation methods to increase artemisinin content in a laboratory scale with biotic or abiotic elicitor. This study examined the effect of elicitation using endophytic fungi Aspergillus sp on artemisinin content of callus A. annua cultures. Observations were made on its growth, texture and color, chlorophyll content and dry weight. Fungi were isolated from the stem tissue of A. annua and callus were grown on Murashige and Skoog medium. After 3 weeks of incubation, callus treated with four different elicitors concentration (0, 2, 4, 6 mg/mL). There were significant alterations on callus culture in terms of growth, texture, color and chlorophyll content after elicitation. The results clearly demonstrated that the endophytic fungal elicitor Aspergillus sp could increase artemisinin content but did not affect the callus dry weight of A. annua. The treatment of 4 mg/mL  elicitor produced the highest artemisinin content, that increased 7.6 times compared to the control, from 0.0036% to 0.0275%.
Effect Balance of Bokashi and Inorganic Fertilizer on Growth, Simplicia Yield, and Content Of Sinensetin of Kumis Kucing (Orthosiphon aristatus (Blume) Miq.) Yoviana Erdhika Adiarti; Bambang Pujiasmanto; Widyatmani Sih Dewi
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 16, No 1 (2019): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v16i1.25102

Abstract

Growth potential, simplicia yields, and secondary metabolites of sinensetin varieties kumis kucing can be increased through fertilization management. The aim of this research was to examine the effect balance of bokashi and inorganic fertilizer on growth, simplicia yields and secondary metabolites of sinensetin kumis kucing varieties. The design of research was a split-plot field experiment, with the main plot were varieties (Orsina 1 and Orsina 2) and subplots in bokashi fertilization of goat manure (control, bokashi 15 tons ha-1, bokashi 15 tons ha-1 + 100% inorganic fertilizer dose recommendation, bokashi 15 tons ha-1 + 50% recommended dosage of inorganic fertilizer and 100% recommended inorganic fertilizer). Inorganic fertilizer recommendations consist of Urea 100 kg ha-1, SP36 200 kg ha-1, and KCl 100 kg ha-1. The results showed that the use of bokashi 15 tons ha-1 + 50% inorganic fertilizer increased growth. Besides it also produced the highest simplicia of 48.57 g plant-1 and the highest secondary metabolite of 0.045% plant-1 in Orsina 1. Both kumis kucing varieties did not show growth differences and yields. Bokashi fertilizer can reduce the use of inorganic fertilizers in the growth and simplicia yields and potentially increase sinensetin of kumis kucing.
Pengaruh Tingkat Adopsi Budidaya Padi Organik terhadap Keberlanjutan Budidaya Padi Organik di Kabupaten Boyolali Putri Permatasari; Sapja Anantanyu; Widyatmani Sih Dewi
Caraka Tani: Journal of Sustainable Agriculture Vol 33, No 2 (2018): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.936 KB) | DOI: 10.20961/carakatani.v33i2.22296

Abstract

Boyolali Regency of Central Java, Indonesia is an area that has developed organic rice cultivation. The development of the adoption rate organic rice cultivation has been conducted since 2009 and still sustainability until today. In fact, not all regions can accept and apply organic rice cultivation. This research was conducted from December 2016 to February 2017 in Boyolali Regency. This research aimed to analyze : (1) the adoption of organic rice cultivation in Boyolali Regency (2) the sustainability of organic rice cultivation in Boyolali Regency (3) the factors affecting the sustainability of organic rice cultivation in Boyolali Regency. This study was quantitative research with explanatory type. The analysis technique employed in this study was a Linear Regression; the sample consisted of 86 farmers living in the Sub Districts having widest organic rice farmland: Sambi, Mojosongo, and Andong. The result of research done by linear regression analysis using SPSS 23,0 program showed that the adoption rate of organic rice cultivation affected directly the sustainability of organic rice significantly with t-test probability (Sign) value of 0,017 less than specified α of 0,05. It indicated that seed preparation, land preparation, seeding, planting, fertilizing and maintenance affected the sustainability of organic rice cultivation in terms of economic and social.
PEMANFAATAN LIMBAH PETERNAKAN DALAM KONSEP PERTANIAN TERPADU GUNA MEWUJUDKAN PERTANIAN YANG BERKELANJUTAN Suryono Suryono; Widyatmani Sih Dewi; Sumarno Sumarno
Caraka Tani: Journal of Sustainable Agriculture Vol 29, No 2 (2014): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.429 KB) | DOI: 10.20961/carakatani.v29i2.13378

Abstract

Utilization of farm wastes in order to realize the concept of integrated farming is a sustainable agriculture community service program in cooperation with two partners, namely : ( 1 ) quail - catfish Breeders 'Agribird', and ( 2 ) dairy farms and agricultural businesses " Andini Mulyo ". This service activities conducted through outreach / awareness, mentoring and manufacture of demonstration plots. Preparation of demonstration plots to make an example of earthworm cultivation ( vermikultur ) and the making of silage. Testing vermicompost and organic fertilizer from cow dung to plant corn and kale using plots measuring 50 x 9 meters, divided into 3 blocks, each 3 x 50 meters. Outcome in the form of products include : Biomass earthworm number 5 Kg / month ; Vermicompost 50 Kg ; Silage, 300 Kg once manufacture ; and quality organic fertilizer from cow dung, 600 kg / process. Outcome in the form of a test product to the plant : the use of manure, vermicompost fertilizer in Litosol very real increase maize crop which includes fresh weight of corn stover, corn stover dry weight and dry weight of seed corn ; the use of manure and fertilizer plants in the ground grumosol not significantly different with kale stover fresh weight, while the use of vermicompost highly significant increase stover fresh weight of spinach.
PENGARUH DOSIS INOKULUM AZOLLA DAN PUPUK FOSFAT ALAM TERHADAP KETERSEDIAAN P DAN HASIL PADI DI ALFISOL Canggih Jati Nusantara; Sumarno Sumarno; Widyatmani Sih Dewi; Sudadi Sudadi
Caraka Tani: Journal of Sustainable Agriculture Vol 29, No 2 (2014): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.996 KB) | DOI: 10.20961/carakatani.v29i2.13396

Abstract

Rice production in Indonesia can not cover the national needs. The low productivity caused by the decline in soil fertility. One way to increase the phosphorus content in the soil is to provide natural phosphate fertilizer and Azolla inoculum. This study was conducted to determine the proper dose of Azolla inoculum and rock phosphate to increase the availability of phosphate and increase the yield of rice plants on land Alfisol. This study uses the two-factor factorial CRD comparative treatment of manure and fertilizer N, P, K. Analysis of the observed data using analysis of variance at 5% level and if there is a significant difference effect followed by DMRT 5% level to compare between treatments. The results showed that treatment of Azolla inoculum and phosphate can increase the availability of phosphate in the Alfisol soil and can increase rice yield. On the parameters available P showed the highest results in the treatment of Azolla 2.5 tons / ha without natural phosphate and Azolla 5 tons / ha of natural phosphate 350 kg / ha which is 10.81 ppm. In addition, administration of Azolla inoculum and phosphate levels give rise to CEC, organic matter, total N in the soil and soil pH.
POTENSI CACING TANAH EKSOTIK ENDOGEIK Pontoscolex corethrurus UNTUK PRODUKSI VERMIKOMPOS GRANUL (VERMIGRAN) BERBASIS BAHAN ORGANIK LOKAL Widyatmani Sih Dewi; Sumarno Sumarno; Sri Rossati
Caraka Tani: Journal of Sustainable Agriculture Vol 27, No 1 (2012): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (873.299 KB) | DOI: 10.20961/carakatani.v27i1.14364

Abstract

The potential of Exotic Earth worm Endogeik (Pontoscolex corethrurus) for Production Vermikompos granules (Vermigran) Local Organil-Based Materials. In 2011, total demand for organic fertilizer in Indonesia was 12.394 million tons and 2.601 million tons of new available. This requirement will continue to increase until 2015, so the opportunity to develop great organic fertilizer. One disadvantage of the use of organic fertilizers is very much in volume (bulky), it is necessary vermikompos manufacturing innovation in the form of granules to be more efficient in the applicationand transport. Research objectives are: (1) study the potential of earthworms in producing vermikompos Pontoscolex corethrurus of l ocal organic ingredients, and (2)produce quality vermikompos in the form of granules. The study was conducted in a greenhouse Fak. Agriculture and in the yard of a house in Ngesrep, Boyolali, in July-November 2011. The study design using a Completely Randomized Design, factorial,two factor. Factor 1 is composed of three types of worms cedar, and the second factoris the variation of the type of organic material, consisting of 7 cedar. The variablesmeasured were vermikompos nutrient quality and speed of the water solubility vermikompos granules. The results showed that Pontoscolex corethrurus high potentialto produce vermikompos that meet ISO quality compost. Cow pile, pile quail, water hyacinth can be used as a raw material vermikompos. Clay, starch, starch and claymixture (1:1) can be used as an adhesive vermikompos granules (vermigran) soluble in water at <48 hours. Production vermigran great potential for developmentas a high-quality organic fertilizer.
Pelapisan Urea dengan Arang Aktif yang Diperkaya Mikroba Indegenus dapat Menurunkan Konsentrasi Endrin Sri Wahyuni; Indratin Indratin; Widyatmani Sih Dewi; Atmanto Heru Wibowo
Bioedukasi: Jurnal Pendidikan Biologi Vol 9, No 1 (2016): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v9i1.3882

Abstract

Endrin residues are still remain in the  land field these compounds are no longer used by farmers and have been banned by the government. This residue can stay in the soil longer and persistant. Microbial enrichment is expected to accelerate the degradation of pesticide residues. Microbes stretcher are Bacillus substillis, Heliothrix oregonensis, Catenococcus thiocycli, and Achoromobacter sp obtained from the preliminary research results  from soil isolation of idegenus in LIPI Cibinong Microbiology Laboratory.  Soil for the planting medium obtained from the village of Karawang, Regency Cilamaya Wetan, Cilamaya District. The experiment was conducted in the field by using lysimeter at the Experiment Jakenan station from July 2013 to December 2013. The objective of the was to obtain technology of activated carbon-coated urea and biochar which  enriched microbial indegenus. The experiment was used randomized block design (RCBD) with 3 replications.  Plant used are rice. Insecticide residue analysis was carried out in the laboratory in Bogor Balingtan using gas wchromatography (GC), with the SNI method 06-6991.1-2004. The purpose of this study knowing the capabilities of urea coating with activated carbon enrichment microbia in reducing the concentration of residues endrin. The  objective were urrea coated activated carbon from coconut shell were enriched with microbes on paddy field can lower pesticide residues of endrin until 33.65%.  This carbon as the preferred home. Enrichmentwith microbial indegenuscan improve theeffectivenessof ureacoating biochar andureacoatingactivated carbon coconut shell todecrease concentration of endrin.
Uji Kualitas Produk Pupuk Organik Beragensia Hayati Mujiyo Mujiyo; Aktavia Herawati; Ganjar Herdiansyah; Suntoro Suntoro; Jauhari Syamsiyah; Widyatmani Sih Dewi; Hery Widijanto; Rahayu Rahayu; Sutarno Sutarno
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 3, No 1 (2022): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v3i1.56302

Abstract

Processing cow dung into organic fertilizer with additional biological agents is an effort to prevent environmental pollution due to agricultural waste. Sources of organic fertilizer materials have different nutrient content. Good quality organic fertilizer means that it has match the Organic Fertilizer Standards based on the Decree of Agriculture Minister Republic Indonesia Number 261/KPTS/SR.310/M/4/2019. This activity aims to determine the quality of the bio-agent organic fertilizer product produced by Perkumpulan Pertanian Organik Wonoagung Wonogiri (PPOWW) by analyzing its nutrient content. The activities carried out include an initial survey of partner conditions, preparation of materials, and analysis of fertilizers. The results show that the PPOWW management understands the mechanism of standard and how to carry out quality control on the organic fertilizers produced. The results of the analysis of the nutrient content of PPOWW organic fertilizer products are N-total 1.32%, P2O5 1.50%, K2O 2.24%, C-organic 23.09%, C/N ratio 17.55 and pH 8.27 which have met the quality standards of organic fertilizers and biological fertilizers. The addition of biological agents in the process of making organic fertilizers is proven to improve the quality of fertilizers.
Co-Authors Achmad Adi Surya Sustama Ahmad Yunus Aisyah Defara Rahmadani AKTAVIA HERAWATI Anak Agung Sagung Putri Risa Andriani Anggita, Akas Arlindo Fernando Macie Arwa Farida Lukito Atmanto Heru Wibowo Atmanto, Heru Bambang Pujiasmanto Canggih Jati Nusantara Canggih Jati Nusantara, Canggih Jati Desti Dian Amalina Dwi Priyo Ariyanto Dwi Priyo Ariyanto Eko Rini Indrayatie Farida Yuliani Ferina Pungky Ganjar Herdiansyah Ganjar Herdiansyah Ganjar Herdiansyah, Ganjar Hardian, Tiara Hasanah, Khalyfah Herwin Setyaningsih Hery Widijanto Ika Ernawati Indratin Indratin Indratin Indratin Irmawati, Viviana Istiqomah, Nanda Mei Jauhari Syamsiyah Jauhari Syamsiyah Komariah K Komariah Komariah Komariah Komariah Krisnawan Kalimutu Kurniatun Hairiah Muhammad Rizky Romadhon Mujiyo Mujiyo Ni Luh Putu Putri Setianingsih Noorcahyati Noorcahyati Novizar Nazir Prabang Setyono Purnomo Edi Sasongko Purwanto Purwanto Purwanto Purwanto PUTRI HANDAYANI Putri Permatasari Rahayu Rahayu Rahayu Rahayu Rahayu Rahayu Ramdan Hidayat Romadhon, Muhammad Rizky Sapja Anantanyu Sri Hartati Sri Rossati Sri Rossati Sri Wahyuni Sri Wahyuni Sudadi Sudadi Sudadi Sudadi Sudjono Utomo Sulandjari Sulandjari Sumani Sumani Sumani Sumani Sumarno . Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Suntoro Suntoro Suntoro Suntoro Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Suryono Suryono Suryono Suryono Sutarno Sutarno Usman Siswanto Vita Ratri Cahyani Wahyu Adi Nugroho Wahyu Galang Pranata Yoviana Erdhika Adiarti