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Population of Phosphate Solubilizing Bacteria and Arbuscular Mycorrhizae in the Rhizosphere of Maize and Peanut in Different Cropping Patterns on Dry Land Ayu Dian Fataya; Widyatmani Sih Dewi; Vita Ratri Cahyani
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4001

Abstract

Dry land has several limiting factors, namely low organic C, low soil fertility, and limited water caused by very low rainfall. Strategies to increase the productivity of dry land can be done by combining the use of organic matter, cover crops, and intercropping crop systems. The existence of functional microbiota including phosphate solubilizing bacteria (PSB) and arbuscular mycorrhiza (AM) could be used as an indicator to evaluate the status of soil health in agricultural dry land areas. The purpose of this study was to analyze and compare the population density and diversity of PSB and AM in the rhizosphere of maize and peanut in monoculture and intercropping dry lands. A descriptive exploration method was conducted by sampling the rhizosphere soil of maize and peanut in the monoculture and intercropping dry lands to analyze the population density and diversity of PSB and AM spores. Each plot was analyzed in 3 replications. The results showed that the rhizosphere soil of maize in the monoculture dry land had the highest population density and diversity of PSB followed by maize in the intercropping dry land. The rhizosphere soil of maize in the intercropping dry land showed the highest AM spore density and diversity followed by maize in the monoculture dry land. So, this finding indicated that maize plants provide more conducive services in dry land.
The Influence of Length of Rehabilitation Process for Ex-Nickel Mining Land on Soil pH, Soil Organic Matter, Population and Distribution of Soil Microbes Timur Priono; Retno Rosariastuti; Sudadi Sudadi; Mujiyo Mujiyo; Widyatmani Sih Dewi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 1 (2025): February 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i1.99-106

Abstract

This study aimed to analyze the influence of rehabilitation length process of the ex nickel mining land on soil pH, SOM, population and soil microbial distribution in East Halmahera, North Maluku, Indonesia. Purposive sampling method was taken under completely randomized design (CRD) with variation of rehabilitation length process: 0, 2, 4, 6, and 8 years. The parameter included soil pH, SOM, population and soil functional bacteria. Data was analyzed using ANOVA and continued by Duncan Multiple Range Test (DMRT) at 5% error level. The result showed the length of rehabilitation process can reduce soil pH, increasing bacteria and fungi population. Four years of rehabilitation process in 2020-Nancy with special treatment of intensive watering resulted the best soil quality characterized by soil pH 6.48, SOM 4.03%, bacteria population of 85 × 10¹ CFU/g, fungi population of 7 × 10¹ CFU/g, non-symbiotic nitrogen-fixing bacteria of 7.0 × 10¹ CFU/g, phosphate-solubilizing bacteria of 0.5 × 10¹ CFU/g, potassium-solubilizing bacteria of 1.0 × 10¹ CFU/g, and proteolytic bacteria of 3.0 × 10¹ CFU/g. The rehabilitation length of 4 years with intensive watering is recommended to provide nutrient and soil microbial on ex-nickel mining rehabilitation. Keywords: Bacteria, Fungi, Nickel, pH, SOM.
Innovation in paddy pest control: Education and implementation of refugia for sustainable agriculture Mujiyo, Mujiyo; Suntoro, Suntoro; Syamsiyah, Jauhari; Dewi, Widyatmani Sih; Rahayu, Rahayu; Widijanto, Hery; Herawati, Aktavia; Herdiansyah, Ganjar; Hasanah, Khalyfah; Anggita, Akas; Irmawati, Viviana; Romadhon, Muhammad Rizky; Hardian, Tiara; Istiqomah, Nanda Mei
Community Empowerment Vol 10 No 5 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.12612

Abstract

Given paddy's status as a staple commodity and primary livelihood in Slogoretno Village, frequently experiencing yield reductions due to pest attacks, this community service activity aimed to educate and implement Integrated Pest Management (IPM) through the planting of refugia plants. The program involved the socialization of local-based refugia concepts, practical planting of perimeter refuge patterns on paddy demonstration plots, and the initiation of agrotourism. The results showed a significant increase in farmers' and the community's knowledge regarding ecological engineering techniques using flowering plants for paddy pest control. Furthermore, these demonstration plots have the potential to become agrotourism objects, which is expected to enhance local welfare and sustainably boost harvest productivity. The implementation of refugia affirms its effectiveness as an IPM innovation supporting sustainable agriculture.
Assessing the Quality of Organic Fertilizer Products Made from Cow Dung in Wonogiri Regency, Indonesia Romadhon, Muhammad Rizky; Mujiyo, Mujiyo; Suntoro, Suntoro; Dewi, Widyatmani Sih; Syamsiyah, Jauhari; Rahayu, Rahayu; Widijanto, Hery; Herdiansyah, Ganjar; Herawati, Aktavia; Anggita, Akas; Hasanah, Khalyfah; Hardian, Tiara; Istiqomah, Nanda Mei; Irmawati, Viviana
Agroindustrial Journal Vol 10, No 2 (2023)
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/aij.v10i2.90130

Abstract

Kebonagung Sub-district, Sidoharjo District, Wonogiri Regency is the Wonoagung Wonogiri Organic Farming Association (PPOWW) location. Mitra is an organization engaged in organic rice and cattle cultivation. The conversion of bovine dung into organic fertilizer, particularly liquid fertilizer containing biological agents, is one of the waste elimination strategies employed to prevent environmental contamination. The nutrient content of organic fertilizer material sources varies. Organic fertilizer of high quality conforms to the Standard for Organic Fertilizers established by the Ministry of Agriculture of the Republic of Indonesia. Only some producers are aware of the standard quality requirements for organic fertilizer. It is necessary to analyze the nutrient content of organic fertilizer products to determine whether the quality of organic fertilizer is excellent. A preliminary survey of partner conditions, preparation of materials (organic fertilizer), and analysis of fertilizer content are among the activities performed. The C/N ratio of organic fertilizer is 8.41, and its pH is 7.6. The analysis results indicate that the Wonoagung Wonogiri Organic Agriculture Association's organic fertilizer products meet the liquid organic fertilizer quality standards. This condition demonstrates that the raw materials used in organic fertilizer production are of high quality and that the appropriate procedures have been followed. Adding biological agents to the production of organic fertilizer can enhance the quality of the finished product.
Impact of Moringa Leaf Liquid Fertilizer on P Uptake and Grain Yield of Organic Rice in Inceptisols Dewi, Widyatmani Sih; Loveana, Okta; Rahmawati, Rani; Sudadi, Sudadi; Purwanto, Purwanto; Rachmawatie, Srie Juli; Cahyono, Ongko
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4067

Abstract

In an effort to solve the P availability issue in Inceptisols, fermentation is one method used to enhance the quality of liqiuid organic fertilizer (LOF) made from Moringa (Moringa oleifera). The type and concentration determine the effectiveness of LOF on nutrient uptake and plant yields. This study aims to evaluate the impact of LOF types, concentrations, and their interactions on P uptake and rice grain yield in Inceptisols. The research was conducted in a greenhouse using a completely random design (CRD) with two factors. The first factor is the type of LOF, consisting of two levels, i.e., fresh and fermented Moringa extract. The second factor is the LOF concentration, with four levels (i.e., 0, 20, 40, 60, and 80 ml/l) and three replications. Data analysis using ANOVA, DMRT, and correlation. The study revealed that the interaction between LOF types and concentrations affects P uptake. The highest P uptake shown by fermented Moringa with a concentration of 60 ml/l was 20.02 mg/plant and 40 ml/l was 18.73 mg/plants., or 1.5 times higher than the control. Grain yield was not affected by type, LOF concentration, or interaction. Fermented Moringa has good potential as LOF, while the effect on grain yield needs further research.
Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Anak Agung Sagung Putri Risa Andriani; Widyatmani Sih Dewi; Novizar Nazir; Ni Luh Putu Putri Setianingsih; Eko Rini Indrayatie; Kalimutu, Krisnawan
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The characteristics of indigenous bacterial isolates in cocoa farms differ based on the study's location and methodology. More research is required to comprehend these bacterial isolates' diversity and features completely. This study intends to assess the cellulose-degrading capacity of bacterial isolates from cocoa plantations in Meko Village, Central Sulawesi. The shape of the bacterial colony, the shape of the colony's edge, elevation, colony size, color, and texture are considered during macroscopic morphological identification and microscopic morphological identification after gram stain. Afterward, gram stains the bacteria to determine the type of cell wall. Hydrolysis capacity was then identified macroscopically by observing colony shape, colony periphery, colony color, colony appearance and height, and hydrolysis capacity on media in a petri dish (HC). Upon microscopic examination of cell shape and bacterial wall type (Gram-positive and gram-negative). Based on the research findings and identification of bacteria capable of degrading cellulose in cocoa soil, it can be inferred that among the 28 examined isolates, six isolates have the greatest HC index values in KL62 isolates. In contrast, GL66, KL23, and KL62x isolates have medium HC index values.
The Influence of Length of Rehabilitation Process for Ex-Nickel Mining Land on Soil pH, Soil Organic Matter, Population and Distribution of Soil Microbes Timur Priono; Retno Rosariastuti; Sudadi Sudadi; Mujiyo Mujiyo; Widyatmani Sih Dewi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 1 (2025): February 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i1.99-106

Abstract

This study aimed to analyze the influence of rehabilitation length process of the ex nickel mining land on soil pH, SOM, population and soil microbial distribution in East Halmahera, North Maluku, Indonesia. Purposive sampling method was taken under completely randomized design (CRD) with variation of rehabilitation length process: 0, 2, 4, 6, and 8 years. The parameter included soil pH, SOM, population and soil functional bacteria. Data was analyzed using ANOVA and continued by Duncan Multiple Range Test (DMRT) at 5% error level. The result showed the length of rehabilitation process can reduce soil pH, increasing bacteria and fungi population. Four years of rehabilitation process in 2020-Nancy with special treatment of intensive watering resulted the best soil quality characterized by soil pH 6.48, SOM 4.03%, bacteria population of 85 × 10¹ CFU/g, fungi population of 7 × 10¹ CFU/g, non-symbiotic nitrogen-fixing bacteria of 7.0 × 10¹ CFU/g, phosphate-solubilizing bacteria of 0.5 × 10¹ CFU/g, potassium-solubilizing bacteria of 1.0 × 10¹ CFU/g, and proteolytic bacteria of 3.0 × 10¹ CFU/g. The rehabilitation length of 4 years with intensive watering is recommended to provide nutrient and soil microbial on ex-nickel mining rehabilitation. Keywords: Bacteria, Fungi, Nickel, pH, SOM.
Penerapan Sistem Persemaian Tray sebagai Upaya Optimalisasi Perbanyakan Benih Padi Kelas Dasar di KB TPH Tegalgondo Supriyadi; Widyatmani Sih Dewi; Faza Nur Afiah; Heca Rahma Safira Aulika Nurhadi; Intan Nur Fitriyani; Kesya Valencia Putri Susanto
Journal of Community Development Vol. 7 No. 1 (2026): August
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v7i1.1919

Abstract

Kebun Benih Tanaman Pangan dan Hortikultura (KB TPH) Tegalgondo memiliki peran strategis dalam penyediaan benih padi kelas dasar (foundation seed) yang berkualitas tinggi. Namun, persemaian konvensional di sawah masih menghadapi kendala efisiensi lahan serta kerentanan terhadap hama dan penyakit, sehingga berpotensi menurunkan kualitas bibit. Kegiatan ini bertujuan untuk menerapkan dan mengoptimalkan sistem persemaian berbasis tray sebagai metode alternatif yang lebih efisien. Kegiatan dilaksanakan pada Januari–Februari 2026 menggunakan pendekatan partisipatif melalui observasi, diskusi teknis, dan praktik langsung dalam seluruh tahapan persemaian. Media tanam berupa campuran tanah aluvial dan pupuk kompos dengan perbandingan 2:1 digunakan dalam tray. Hasil menunjukkan bahwa sistem tray menghasilkan bibit yang lebih seragam, memiliki sistem perakaran yang lebih kuat, serta mengurangi kerusakan saat pindah tanam. Penggunaan benih sekitar 60 gram per tray mendukung kepadatan optimal dan mempermudah pengelolaan persemaian. Selain itu, metode ini meningkatkan efisiensi tenaga kerja dan pengendalian kondisi tumbuh. Secara keseluruhan, sistem persemaian tray berpotensi meningkatkan kualitas bibit dan efisiensi operasional dalam perbanyakan benih padi
Effect of Microbial Fuel Cell, fertilizer, and plant spacing on nitrogen dynamics in paddy soil Efendi, Syahrul; Komariah, Komariah; Syamsiyah, Jauhari; Dewi, Widyatmani Sih; Hiramatsu, Ken; Azhar Fauzan, Adhia
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 21, No 1 (2024): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v21i1.86312

Abstract

Nitrogen is one of the primary nutrients required for growing rice. Still, the efficiency of urea fertilizer application is very low (20-40%) due to the nitrogen loss process, one of which is denitrification. This study aims to determine the effects of combining Microbial Fuel Cell (MFC), plant spacing, and fertilization on nitrogen dynamics in paddy fields. The combination of treatments are expected to reduce the nitrogen loss in paddy fields, and plants can absorb it efficiently. A total of six treatments included Microbial Fuel Cell (MFC) (2 levels: without MFC and with MFC), plant spacing (2 levels: conventional spacing 25 cm × 25 cm and jajar legowo spacing 25 cm × 12.5 cm × 50 cm), and fertilization (2 levels: without fertilizer and with 500 kg ha-1 of NPK fertilizer), with three replications for each combination. The observed parameters included total soil nitrogen, nitrate, nitrogen uptake, chlorophyll, nitrogen-fixing and denitrifying bacteria, and N2O gas emissions. The results showed that combining MFC, conventional spacing, and NPK fertilizer in the paddy fields resulted in a high total soil nitrogen (0.44%). The results showed different effects on total soil nitrogen in the MFC and fertilization treatments, leading to increased nitrate levels, nutrient uptake, and chlorophyll. Increasing total soil nitrogen significantly contributes to leaf development and significantly aids photosynthesis. The integration of MFC and fertilization observed in this study resulted in a real impact on nitrogen dynamics in paddy fields. This combined treatment effectively reduces total nitrogen loss due to denitrification in paddy fields, thereby increasing the efficiency of uptake by plants.
Viability of Agrobacterium sp. I26 and Agrobacterium sp. I30 in The Carrier Formula of Mocaf Solid Waste, Peat and Manure Rosariastuti, Retno; Nur Sabilla, Widya Putri; Sudadi, Sudadi; Dewi, Widyatmani Sih
ENVIRO: Journal of Tropical Environmental Research Vol 24, No 2 (2022): September
Publisher : Center for Environmental Research, LPPM, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/enviro.v24i2.70354

Abstract

Agrobacterium sp. I26 and Agrobacterium sp. I30 are bioremediation agent that can be used as functional bacteria in biofertilizers. The storage of these bacteria requires carrier. Not all carriers can support bacterial viability, so it was necessary to examine carrier formulas as basic ingredients for biofertilizers which the quality standards based on the Minister of Agriculture Regulation No. 70/2011. This research aimed to: (1) study the viability of Agrobacterium sp. I26 and Agrobacterium sp. I30 in some carrier formulas; and (2) obtain the best carrier formula to support the viability of Agrobacterium sp. I26 and Agrobacterium sp. I30. Research design was factorial using completely randomized design (CRD) as the based design, consisting of 2 factors: 1) Carrier Fornula (C): C1; C2; C3; C4; C5, 2) Bacteria (I): I1; I2; I3, so there were 15 treatment combinations each was repeated 4 times, thus there were 60 experimental units. The results showed that Agrobacterium sp. I26 has better viability than Agrobacterium sp. I30 during 90 days incubation period. The best bacterial viability with total bacterial as the indicator was C4 carrier formula: 74 x 10­13 cfu.g-1 for Agrobacterium sp. I26 and C3 carrier formula: 155 x 1012 cfu.g-1.
Co-Authors Achmad Adi Surya Sustama Ahmad Yunus Aisyah Defara Rahmadani AKTAVIA HERAWATI Anak Agung Sagung Putri Risa Andriani Anak Agung Sagung Putri Risa Andriani Anggita, Akas Arlindo Fernando Macie Arwa Farida Lukito Atmanto Heru Wibowo Atmanto, Heru Ayu Dian Fataya Azhar Fauzan, Adhia Bambang Pujiasmanto Canggih Jati Nusantara Canggih Jati Nusantara, Canggih Jati Desti Dian Amalina Dwi Priyo Ariyanto Efendi, Syahrul Eko Rini Indrayatie Farida Yuliani Faza Nur Afiah Ganjar Herdiansyah, Ganjar Hardian, Tiara Hasanah, Khalyfah Heca Rahma Safira Aulika Nurhadi Herwin Setyaningsih Hery Widijanto Hiramatsu, Ken Ika Ernawati Indratin Indratin Indratin Indratin Intan Nur Fitriyani Irmawati, Viviana Istiqomah, Nanda Mei Jauhari Syamsiyah Kalimutu, Krisnawan Kesya Valencia Putri Susanto Komariah K Komariah Komariah Komariah Komariah Krisnawan Kalimutu Kurniatun Hairiah Loveana, Okta MMA Retno Rosariastuti Muhammad Rizky Romadhon Mujiyo Mujiyo Ni Luh Putu Putri Setianingsih Ni Luh Putu Putri Setianingsih Noorcahyati Noorcahyati Novizar Nazir Nur Sabilla, Widya Putri Nuraini Dwi Agustina Putri Ongko Cahyono Prabang Setyono Pungky Slamet Wisnu Kusuma Purnomo Edi Sasongko Purwanto Purwanto Purwanto Purwanto Purwanto Purwanto PUTRI HANDAYANI Putri Permatasari Rachmawatie, Srie Juli Rahayu Rahayu Rahayu Rahayu Rahayu Rahayu Ramdan Hidayat Rani Rahmawati Retno Rosariastuti Retno Rosariastuti Romadhon, Muhammad Rizky Sapja Anantanyu Slamet Minardi Slamet Minardi Sri Hartati Sri Rossati Sri Rossati Sri Wahyuni Sri Wahyuni Sudadi Sudadi Sudadi Sudadi Sudadi Sudadi Sudjono Utomo Sulandjari Sulandjari Sumani Sumani Sumani Sumani Sumarno . Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Suntoro Suntoro Suntoro Suntoro Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Suryanti Suryanti Suryono Suryono Suryono Suryono Sutami Sutami Sutarno Sutarno Timur Priono Usman Siswanto Vita Ratri Cahyani Vita Ratri Cahyani Vita Ratri Cahyani Wahyu Adi Nugroho Wahyu Galang Pranata Yoviana Erdhika Adiarti