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All Journal Jurnal Veteriner Jurnal Teknologi Laboratorium Journal of Health (JoH) Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Journal of Health Sciences Medical Technology and Public Health Journal Medical Laboratory Analysis and Sciences Journal JURNAL ANALIS LABORATORIUM MEDIK Jurnal Riset Biologi dan Aplikasinya Sains dan Matematika Medicra (Journal of Medical Laboratory Science/Technology) Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Jurnal Pengabdian Masyarakat dalam Kesehatan Humanism : Jurnal Pengabdian Masyarakat Jurnal Abdimas Kartika Wijayakusuma Indonesian Journal of Innovation Studies Manajemen Pelayanan Kesehatan Indonesian Journal Health Science and Medicine
Miftahul Mushlih
Universitas Muhammadiyah Sidoarjo
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Comparison the Quality of Template DNA isolated by Column Method with and without Centrifugation: Perbandingan Kualitas DNA Template yang diisolasi dengan Metode Kolom dengan dan tanpa Sentrifugasi Wardana, Aji Cakra; Mushlih, Miftahul
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.129 KB) | DOI: 10.21070/ijins.v15i.552

Abstract

DNA isolation is the first step in molecular biology work. The quality of the DNA template is very important because it can affect the DNA amplification reaction. The application of centrifugation to the sample is expected to produce a Buffy coat layer containing nuclear cells. This study aims to compare the quality of the DNA template isolated from the column with and without centrifugation. This research used descriptive experimental method. The samples used were 16 samples with the division of 8 samples given centrifugation treatment and 8 samples without centrifugation. DNA isolation column method is a DNA isolation method that uses a silica membrane, where the silica membrane works as a filter for DNA that is split from the cell. The DNA isolation method column was carried out according to the standard protocol on the Geneaid DNA mini Kit Blood/Tissue. The concentration and purity of DNA were measured using UV-Vis Spectrophotometry at 260 nm and 280 nm. Visualization of DNA isolation results was carried out using 1% agarose gel. The results of measuring the quality of DNA samples using the column method with centrifugation showed better results with clearer visualization of DNA bands than samples without centrifugation. The results of the T dependent statistical test on both samples of p value < 0.05 showed that there was a significant difference in DNA quality with centrifuged and non-centrifuged treatment.
Differences in DNA Purity Test Using UV-Vis Spectrophotometer and Nanodrop Spectrophotometer in Type 2 Diabetes Mellitus Patients: Perbedaan Uji Kemurnian DNA Menggunakan Spektrofotometer UV-Vis dan Spektrofotometer Nanodrop pada Pasien Diabetes Melitus Tipe 2 Dewanata, Pandu Aji; Mushlih, Miftahul
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.128 KB) | DOI: 10.21070/ijins.v15i.553

Abstract

DNA quality test is one of the basic techniques in Molecular Biology which aims to determine the presence or absence of protein and RNA contamination. DNA quantity test can be done using a spectrophotometer. Spectrophotometer is a tool used to measure the concentration of a compound based on its ability to absorb light. The purpose of this study was to determine differences in DNA purity as measured by UV-Vis Spectrophotometer and Nanodrop Spectrophotometer. The method used in this research is descriptive exploratory with purposive sampling technique. The number of samples used is 15 samples. The results of this study are that there are differences in the concentration and purity of DNA from quantitative tests with UV-Vis spectrophotometers and Nanodrops obtained based on statistical tests using the T Dependent Test. The result of this research is that in measuring DNA purity there is a difference in DNA purity because the results of Sig (2-Tailed) use 0.001 or below 0.05 (Ha is accepted). And based on statistical tests on DNA concentration measurements, it is known that there are differences in DNA concentrations.
IDENTIFIKASI MUTASI GEN TRANSCRIPTION FACTOR 7 LIKE 2 (TCF7L2) SNP RS7901695 PADA PENDERITA DIABETES MELITUS TIPE-2 MENGGUNAKAN PCR Azhar, Risya Auliya' Putri; Mushlih, Miftahul
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i2.6378

Abstract

Latar belakang: Diabetes Melitus Tipe-2 (DMT2) merupakan penyakit metabolik kronis yang dipengaruhi oleh faktor genetik, salah satunya adalah mutasi pada gen TCF7L2, khususnya SNP rs7901695. Tujuan: untuk mendeteksi mutasi SNP rs7901695 pada penderita DMT2. Metode: Polymerase Chain Reaction (PCR) secara langsung yang sederhana dan efisien. Sebanyak 30 sampel darah pasien DMT2 dianalisis menggunakan primer spesifik untuk alel T (177 bp) dan alel C (367 bp). Sebagian besar pasien berada pada kelompok usia 50-59 tahun, yang mengindikasikan pentingnya deteksi dini pada usia produktif.  Hasil: elektroforesis menunjukkan 28 sampel (93,3%) memiliki genotipe heterozigot TC dan 2 sampel (6,7%) genotipe homozigot TT, tanpa adanya genotipe CC. Kesimpulan: PCR langsung dapat digunakan sebagai metode awal yang efektif dan ekonomis dalam identifikasi mutasi genetik DMT2, serta mendukung skrining genetik berbasis populasi untuk pencegahan yang lebih personal.
Co-Authors Ahmad Shobrun Jamil Aini, Aqiliyanti Nur Alifia Nurfitriana Amin, Hindah Sabrina Andika Aliviameita Andika Aliviameyta Anggie Wedha Puspita Apriliana, Dinda ARDIANSYAH, SYAHRUL Arief Wisaksono Aulia, Faradila Nur Azhar, Risya Auliya' Putri Bayu Segara Budi Setiadi Daryono Chylen Setiyo Rini Dewanata, Pandu Aji Dian Delta Dina Trikumalasari Djauharoh Djauharoh Evi Rinata Firda, Aminatul Fitri Kumala Sari Fitrian Desi Prameswari Galuh Ratmana Hanum Hadie, Djauharoh A Hindah Sabrina Amin Huesein, Iknan, Siti Asriani Imamah, Siti Aliyatul Jamilatur Rohmah Jayanti, Liah Dwi Kartikasari, Erina Wahyu Kurnia Wahyu Ningsih Livia Nur Azzah Liyaajul, Pratasyah Meryn Linanda Agustin Nia Lukita Arian NUR EFENDI NURUL AZIZAH Nurul Azizah Paramitha Amelia Kusumawardani Puspitasari Puspitasari Puspitasari Puspitasari Puspitasari Qilmia Fanani Rohman Dijaya Sari, Azizah Krismonita Sari, Fitri Kumala Segara, Bayu Siska Aprilia Siti Asriani Iknan Siti Cholifah Tis’iyyah , Siti Nur Maghfiroh Wardana, Aji Cakra Yanik Purwanti Zakaria, Rizal