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All Journal KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Media Ilmu Keolahragaan Indonesia International Journal of Education Jurnal Keolahragaan Jurnal SPORTIF : Jurnal Penelitian Pembelajaran Wellness And Healthy Magazine SMART SPORT Narra J Journal of Multidisciplinary Applied Natural Science Indonesian Journal of Physical Education and Sport Science (IJPESS) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Prosiding University Research Colloquium Physical Therapy Journal of Indonesia Journal of Social Work and Science Education Jurnal Info Kesehatan
Muchsin Doewes
Faculty of Medicine, Sebelas Maret University
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Effects of aerobic exercise and high-intensity interval training on muscle damage in an overtraining rat model Syetiawinanda, Amriansyah; Doewes, Muchsin; Purwanto, Bambang; Soetrisno, Soetrisno; Kristiyanto, Agus; Pamungkasari, Eti Poncorini
Physical Therapy Journal of Indonesia Vol. 6 No. 1 (2025): January-June 2025
Publisher : Universitas Udayana dan Diaspora Taipei Medical University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/ptji.v6i1.297

Abstract

Background: Overtraining is commonly associated with elevated oxidative stress, inflammatory responses, and structural damage to muscle fibers, all of which contribute to a decline in physical performance. This study aimed to assess the impact of aerobic exercise on biomarkers including malondialdehyde (MDA), interleukin-6 (IL-6), caspase-3, nitric oxide (NO), superoxide dismutase (SOD), as well as the integrity of the sarcolemma. Methods: A six-week experimental study was conducted using 24 rats, which were randomly divided into three groups (n = 8 per group): a negative control group (no treatment), an aerobic exercise group, and a high-intensity interval training (HIIT) group. The levels of biochemical markers, including malondialdehyde (MDA), interleukin-6 (IL-6), nitric oxide (NO), and superoxide dismutase (SOD), were measured using the enzyme-linked immunosorbent assay (ELISA) technique. Additionally, the expression of caspase-3 was determined through immunohistochemical (IHC) analysis. Muscle tissue damage was evaluated by means of histopathological examination. The data were analyzed using a one-way analysis of variance (ANOVA), followed by the Mann-Whitney post hoc test to assess differences between groups. Results: The findings indicated that aerobic exercise did not result in a statistically significant reduction in malondialdehyde (MDA) (p = 0.833), interleukin-6 (IL-6) (p = 0.800), nitric oxide (NO) (p = 0.791), or superoxide dismutase (SOD) (p = 0.520) levels. Nevertheless, the aerobic exercise group exhibited a significantly lower expression of caspase-3 compared to the other groups (p = 0.023). Furthermore, aerobic exercise was associated with a significant reduction in muscle tissue damage (p = 0.001). Conclusion: The findings of this study indicate that both aerobic exercise and high-intensity interval training (HIIT) have the potential to be utilized as therapeutic approaches for mitigating exercise-induced muscle damage, primarily through their positive influence on sarcolemma stability. However, additional clinical investigations are necessary to confirm these results and establish their relevance for clinical implementation.
Battery of tests for return-to-sport assessment after knee injury in athletes Lesmana, Syahmirza Indra; Sugiyanto; Hidayatullah, M. Furqon; Doewes, Muchsin; Jusup, Sinu Andhi
Physical Therapy Journal of Indonesia Vol. 6 No. 2 (2025): July-December 2025
Publisher : Universitas Udayana dan Diaspora Taipei Medical University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/ptji.v6i2.285

Abstract

Background: Knee injury is a common sports injury, and exercise therapy can be guided by functional and biological tests to assess healing and readiness to return to sport. This study aimed to develop a test battery to identify the most predictive functional test in determining the success of an exercise therapy program for sports injury. Methods: A test construction methodology was used, incorporating 8 field test instruments and TGF-β1 as a biological marker. Statistical methods, including multiple correlation and Wherry Doolittle test selection, were applied to develop a functional test model supporting post-injury sports therapy. Results: This study found no significant correlation between TGF-β1 and the single leg stance (p=0.160), single leg calf raise (p=0.902), vertical jump (p=0.344), shuttle run (p=0.794), and hop tests (p=0.555). However, significant correlations were found with the sit and reach test (p<0.001; r=0.471) and hexagonal agility test (p=0.019; r=-0.318). Multivariate linear regression showed that the single leg stance, sit and reach, and hexagonal agility tests were predictors of TGF-β1, with the single leg stance and sit and reach tests explaining 23% of its variance. Conclusion: Test battery arrangement for functional tests that were considered predictive include single leg stance and sit and reach tests, with low correlation values, which two tests in the form of single leg balance and sit and reach tests have a relationship with tissue healing. Additionally, several other tests were designed to determine the ability of athletes to RTS.
The effects of overtraining protocol on markers of oxidative stress and inflammation Syetiawinanda, Amriansyah; Doewes, Muchsin; Purwanto, Bambang; Soetrisno, Soetrisno; Kristiyanto, Agus; Pamungkasari, Eti Poncorini
Physical Therapy Journal of Indonesia Vol. 6 No. 2 (2025): July-December 2025
Publisher : Universitas Udayana dan Diaspora Taipei Medical University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/ptji.v6i2.304

Abstract

Background: Overtraining can elevate reactive oxygen species (ROS), suppress endogenous antioxidant activity, and trigger systemic inflammation. Monitoring oxidative stress and inflammation is essential to prevent muscle damage. This study aimed to examine the effects of an overtraining protocol on oxidative stress and inflammatory markers MDA, IL-6, NO, and SOD in male Wistar rats. Methods: The experimental was conducted over two weeks using male Wistar rats at the Bioscience Institute Laboratory, Universitas Brawijaya, Malang, Indonesia as a preliminary study. Three rats were examined to assess the effects of overtraining on MDA, IL-6, NO, and SOD levels. Observations were made one day after overtraining protocol for the intervention group value (KP) and six weeks after overtraining for the control group value (K1). Results: Three male rats underwent an overtraining protocol, and blood samples were collected one day post-overtraining (KP) to assess MDA, IL-6, NO, and SOD levels. The mean values at KP were MDA: 370.3 ± 10.3, IL-6: 5.9 ± 0.7, NO: 61.0 ± 8.4, and SOD: 0.05 ± 0.03. At six weeks post-overtraining (K1), MDA (192.8 ± 10.3), IL-6 (3.3 ± 1.9), and NO (41.7 ± 21.6) levels were lower, while SOD (0.3 ± 0.2) was higher compared to KP. Conclusion: Overtraining can elevate ROS and inflammatory responses, as indicated by increased MDA, IL-6 levels, and NO, along with reduced SOD expression.
Student Learning Motivation in Physical Education Learning Based on Physical Activity: A High School Analysis Study Duta Putra Utama, Dimas; Doewes, Muchsin; Fajar Ekawati, Febriani; Németh, Zsolt
Indonesian Journal of Physical Education and Sport Science Vol. 3 No. 2 (2023): September: Indonesian Journal of Physical Education and Sport Science
Publisher : Indonesian Journal of Physical Education and Sport Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52188/ijpess.v3i2.445

Abstract

Study Purpose. Articulate the key intersection points and differences between motivational indicators that can influence the occurrence of motivation to learn in physical education learning at school. This study was designed to examine the effect of physical activity participation in organized schools on indicators of learning motivation in physical education learning. Materials and methods. Assessment of physical activity based on a questionnaire using the International Physical Activity Questionnaire (IPAQ) and motivation to learn physical education based on research on six indicators that have passed the Expert Judgment stage. Participants consisted of 202 grade 10 students who attended physical education classes at SMAN 1 Sukoharjo, Central Java. The greater the effect of physical activity on participating students to increase the overall learning motivation is higher. Perceptions of learning motivation indicators related to physical activity items. Results. In both of these influence assessments, indicators of learning motivation can be said to be influenced by several physical activity items. Changes in physical activity can predict changes in learning motivation, except for Learning Tools and Teaching Methods. In particular, physical activity items can affect indicators of students' environmental conditions. Conclusion. There is a dynamic influence between the participation of learning motivation indicators in physical education learning on student activities at school.
Studi Evaluasi Latihan Kondisi Fisik Pada Atlet Bulutangkis Pemusatan Pendidikan Dan Latihan Olah Raga Pelajar (PPLOP) Jawa Tengah Tahun 2017/2018 Aprilia, Khalida Nawa; Kristiyanto, Agus; Doewes, Muchsin
Prosiding University Research Colloquium Proceeding of The 7th University Research Colloquium 2018: Bidang Pendidikan, Humaniora dan Agama
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar belakang penelitian adalah untuk mengetahui sistematikaprogram latihan kondisi fisik atlet PPLOP Bulutangkis Jawa Tengahdengan menerapkan prinsip-prinsip latihan. Adapun jenis prinsipprinsiplatihan tersebut yaitu prinsip individual, prinsip spesifikasi,prinsip overload, prinsip progresif, prinsip reversibility, dan prinsiprecovery.Metode penelitian yang digunakan dalam penelitian ini adalahpenelitian evaluasi program, yang bertujuan untuk melihat apakahprogram dirancang, dilaksanakan, dan bermanfaat bagi pihak-pihakyang terlibat dalam program. Pada pelaksanaannya evaluasi programbermaksud mencari informasi sebanyak mungkin untuk mendapatkangambaran rancangan dan pelaksanaan program. Pendekatan yangdigunakan dalam penelitian ini adalah pendekatan kualitatif dandidukung dengan penekatan kuantitatif. Subyek dalam penelitian iniadalah atlet dan pelatih PPLOP Bulutangkis Jawa Tengah yang terdiridari 2 pelatih dan 4 atlet putra dan 4 atlet putri.Hasil penelitian dari Studi Evaluasi Latihan Kondisi Fisik pada AtletBulutangkis Pemusatan Pendidikan dan Latihan Olah Raga Pelajar(PPLOP) Jawa Tengah Tahun 2017/2018 ini adalah : Pertama, hasilwawancara pada atlet dan pelatih menunjukkan bahwa untuk prinsiprecovery pada saat latihan dan selesai latihan kurang diperhatikan.Sehingga yang terjadi adalah tingkat kelelahan pada atlet belumsepenuhnya pulih dan mengakibatkan hasil latihan kurang maksimal.Kedua, hasil observasi pada atlet dan pelatih menunjukkan bahwasecara garis besar program latihan yang diterapkn pada PPLOPBulutangkis Jawa Tengah masuk pada kreteria baik yaitu 81,83% inimerupakan perolehan hasil dari angket observasi pelatih dan atlet.Akan tetapi pada tabel observasi atlet dan pelatih menunjukan bahwapada prinsip recovery dan overload menunjukan nilai yang masihdalam kreteria rendah yaitu untuk overload 73.75% dan untukrecovery 71.66 %, sehingga untuk prinsip overload dan recovery padaprogram latihan di PPLOP Bulutangkis harus mendapatkanperbaikan, agar kondisi fisik atlet dapat mencapai pada tingkat yangmaksimal yang akan berdampak pada prestasi atlet tersebut. Darihasil data yang diperoleh melalui wawancara dan observasi padapelatih dan atlet PPLOP Bulutangkis Jawa Tengah Tahun 2017/2018,maka peneliti melakukan perbaikan pelaksanaan program latihankondisi fisik dengan berdasarkan pada prinsip-prinsip latihan. Ketiga,hasil peningkatan kondisi fisik atlet PPLOP Bulutangkis Jawa TengahTahun 2017/2018 setelah mendapatkan perbaikan pada programlatihan fisik melalui prinsip overload dan prinsip recovery adalah
HIIT (High-Intensity Interval Training) is an Alternative Solution to Reduce Obesity Savira, Intan; Doewes, Muchsin; Rohma, Noer
Prosiding University Research Colloquium Proceeding of The 11th University Research Colloquium 2020: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Penelitian ini bertujuan untuk mempromosikan program latihan HIIT sebagai suatu solusi alternative mengatasi kelebihan lemak pada penderita obesitas. Metode penelitian yang digunakan yaitu metode kepustakaan yang berfokus pada masalah obesitas dan latihan yang digunakan untuk mengatasi permasalahan yang terkait obesitas. Penelitian literatur adalah penelitian yang dilakukan dengan membaca dan mengumpulkan referensi yang relevan dari buku referensi, jurnal ilmiah, dan manuskrip. Hasil menunjukkan bahwa latihan HIIT memberikan efek yang lebih efektif untuk mengendalikan lemak bagi penderita kelebihan berat badan.
The Effect of Dayak Onion Extract (Eleutherine palmifolia (L.) Merr) on Swimming Time and Oxidative Stress Levels in Mice with the Forced Swimming Test Model Ramadhani, Aulia; Doewes, Muchsin; Listyawati, Shanti
JURNAL INFO KESEHATAN Vol 21 No 3 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss3.1144

Abstract

Excessive physical exercise can increase the occurrence of oxidative stress, which is characterized by cell damage caused by free radicals. Antioxidants found in Dayak Onion such as flavonoids, saponins, tannins, and vitamin C can be used as alternative ingredients to neutralize free radicals. The purpose of this study was to determine the effect of dose and duration of administration of Dayak onion extract on malondialdehyde (MDA) levels in a rat model of the forced gum test, extraction of Dayak onions using 96% ethanol. The type if research is Randomized Pre and Post Test Control Group Design. Male Sprague Dawley rats aged 2 months (weighing 150-200g) were divided into 5 groups: K- (control group, rats not given Dayak Onion extract but underwent forced swimming test), K+ (rats given xanthine and forced swimming test), P1 (rats given Dayak Onion extract at a dose of 50 mg/200 rats body weight/day), P2 (rats given Dayak Onion extract at a dose of 100 mg/200 rats body weight/day), P3 (rats given Dayak Onion extract at a dose of 200 mg/200 rats body weight/day). Dayak Onion extract was administered for 21 days with for 21 days with forced swimming test treatment, and changes in MDA levels were observed. The results showed that the administration of Dayak onion extract at a dose of 50 mg/200 rats/day, 100 mg/200 rats/day, and 200 mg/200 rats/day had an effect on preventing the increase in MDA, but at a dose of 200 mg/200 rats mice/day is the optimal dose. The conclusion is administration of Dayak Onion (Eleutherine palmifolia (L.) Merr) significantly decreased MDA levels in male Sprague Dawley rats with forced swimming test model.
Implementasi pendidikan jasmani dalam international primary curriculum Nugroho, Kristanto Adi; Kristiyanto, Agus; Doewes, Muchsin
Jurnal Keolahragaan Vol. 6 No. 2: September 2018
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.6 KB) | DOI: 10.21831/jk.v0i0.21336

Abstract

Tujuan penelitian ini adalah mengetahui keterkaitan dan implementasi pendidikan jasmani dalam International Primary Curriculum (IPC) sebagai salah satu kurikulum internasional. IPC adalah kurikulum yang komprehensif, tematik, dinamis dan berwawasan global. Pelaksanaan kurikulum terstruktur dan terencana memungkinkan siswa untuk beradaptasi dengan tantangan global di masa mendatang. Metode penelitian adalah metode penelitian kualitatif deskriptif. Hasil penelitian, pelaksanaan pendidikan jasmani di IPC telah ditentukan oleh sekolah melalui data dengan pemutakhiran terbaru. Tema yang dipilih kemudian disesuaikan dengan tujuan sekolah, budaya lokal, kebutuhan siswa. Kesimpulannya adalah IPC sangat dinamis sehingga dapat diselaraskan untuk mencapai tujuan sekolah yang ingin dicapai dengan menyesuaikan budaya budaya lokal, kondisi siswa dan sekolah. AbstractThe purpose of this research is determine relationship and implementation of physical education in International Primary Curriculum (IPC) as an international curriculum. IPC is comprehensive, thematic, dynamic and global-minded curriculum. The implementation of a structured and planned curriculum allows students to adapt to global challenges in the future. The research method is descriptive qualitative research. As a result, implementation of physical education in IPC has been determined by school through the latest update data. The choosen theme is then adjusted to school goals, local culture, student needs. The conclusion is that IPC is very dynamic so that it can be harmonized to achieve the school goals by adjusting the culture of the local culture, conditions of students and schools.
The Biochemical and Kidney Histopathological Parameters in Hyperoxaluria Rats Treated with Breadfruit Leaf Extract Susilo, Jatmiko; Purnama, Siswanta Jaka; Wahyuni, Ardianti; Purwanto, Bambang; Doewes, Muchsin; Indarto, Dono
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1437

Abstract

Purpose: This study was to analyze the administration of breadfruti leaf extract to changes in biochemical parameters and renal histology in hyperoxaluria rats.Methodology: The experimental study used 6 groups, each of 5 mice that are EG, Normal, Vitamin E, an ethanol extract of breadfruit 100, 200 and 400 mg/kg BW. Blood data for the analysis of biochemical parameters and kidney were taken at the end of the experiment for histological analysis of kidney changes. Results: Research shows that the purified ethanol extract of breadfruit leaf can prevent increases in MDA levels, reduce creatinine and BUN levels, increase body weight, prevent hypertrophy and kidney damage Conclusion: Purified ethanol extract of AA leaves has an antioxidant, nephroprotective activity, protect kidney inflammation, and kidney damage in nephrolithiasis rats and the effective dose is 400 mg/kg BW. The higher the extract dose, the more effective to reduce the biochemical and histopathological parameters in hyperoxaluria rats.Applications/Originality/Value: as a basis for futher studies of molecular biology in the prevention of kidney disorder.
Efek latihan peregangan pada fleksibilitas bagi siswa Hidayatullah, Muhammad A'raaf; Doewes, Muchsin; Kunta Purnama, Sapta
Jurnal SPORTIF : Jurnal Penelitian Pembelajaran Vol 8 No 1 (2022): Jurnal SPORTIF: Jurnal Penelitian Pembelajaran
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1702.282 KB) | DOI: 10.29407/js_unpgri.v8i1.17742

Abstract

Flexibility is an important basic component in sports activities, which supports the achievement of the maximum performance of an athlete. Exercises are needed for stretching proper this study aimed to determine: (1) the effect of passive static stretching on shoulder flexibility, (2) the effect of Proprioceptive Neuromuscular Facilitation (PNF) stretching on shoulder flexibility (3) determine which exercise is better on shoulder flexibility between stretching and PNF. the method used is experimental with pretest and post-test design. The research sample was 16 students of the Sport Coaching Education study program, Faculty of Sport, Sebelas Maret University, Surakarta. research data was obtained using the sit and reach test. Data analysis using an independent sample test with the help of the SPSS 22.0 program. The results of the study (1) passive static stretching effect on togok flexibility, the test results show the value of sig. 0.00 < 0.05. (2) practice PNF affects the flexibility of stakes. The study results show that both passive tactical stretching exercises and PNF exercises have a significant effect on increasing flexibility, but empirical data states that PNF exercise is more effective in increasing flexibility.
Co-Authors . Fadityo . Kisrini . Samigun Abdurrachman, Abdurachman Agus Kristiyanto Akbar, Muhammad Thoriq Hadad Alifiyanti Muharramah ANAS KHOLIKUL AMIN, ANAS KHOLIKUL Annisa Marsha Evanti Aprilia, Khalida Nawa Aulia Ramadhani Bambang Purwanto Bhisma Murti Didik Gunawan Tamtomo Dono Indarto Duta Putra Utama, Dimas Ekawati, Febriani Fajar Eti Poncorini Pamungkasari Fajar Ekawati, Febriani Hari Hanggoro, Hari Hartono Hartono Hidayatullah, Muhammad A'raaf Ida Nurwati Jatmiko Susilo Khoirul Huda Kisrini . M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Muh. Furqon Hidayatullah Németh, Zsolt NINGRUM, TYAS SARI RATNA Nugroho, Kristanto Adi Nur Zahratul Jannah Pamungkasari, Eti P. Paramasari Dirgahayu pasa, chusairil Purnama, Siswanta Jaka Ratih Puspita Febrinasari Restian Gigih sejati, Restian Gigih Risya Cilmiaty, Risya Riza Setya Agrensa Rohma, Noer Rony Syaifullah, Rony Sapta Kunta Purnama Savira, Intan Shanti Listyawati Sinu Andhi Jusup, Sinu Andhi Slamet Riyadi Soetrisno Soetrisno Sopianti, Densi Selpia SUGIYANTO Sumandjar, Tatar Syahmirza Indra Lesmana Syetiawinanda, Amriansyah Utama, Dimas Duta Putra Vitri Widyaningsih Wahyuni, Ardianti