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All Journal Bioscience Jurnal Pendidikan Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Martabe : Jurnal Pengabdian Kepada Masyarakat Eksakta : Berkala Ilmiah Bidang MIPA Pelita Eksakta Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi Edumaspul: Jurnal Pendidikan
Violita Violita
Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Social Sciences Transportation Other

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Analisis Kualitas Soal Ujian Tengah Semester Biologi Kelas XII SMA Negeri 6 Padang Tahun Pelajaran 2021/2022 Sintia Putri; Zulyusri Zulyusri; Violita Violita
Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Vol 4, No 1 (2022): Metaedukasi
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/metaedukasi.v4i1.4090

Abstract

Penelitian ini bertujuan untuk menggambarkan kualitas soal Ujian Tengah Semester Biologi Kelas XII SMA Negeri 6 Padang Tahun Pelajaran 2021/2022. Penelitian ini menggunakan pendekatan deskriptif kuantitatif. Teknik pengumpulan data yang digunakan pada penelitian ini adalah teknik dokumentasi berupa lembar soal Ujian Tengah Semester mata pelajaran Biologi dengan bentuk soal pilihan ganda berjumlah 25 butir, kunci jawaban soal ulangan harian dan respon siswa sebanyak 33 orang siswa. Data yang terkumpul kemudian dianalisis secara kuantitatif untuk memperoleh informasi terkait validitas, reliabilitas, tingkat kesukaran, daya pembeda, dan pengecoh. Teknik analisis data menggunakan SPSS versi 16 untuk melihat validitas dan reliabilitas dan Anates versi 4 untuk melihat tingkat kesukaran, daya pembeda, dan pengecoh. Hasil dalam peneltian menunjukkan bahwa, reliabilitas soal yaitu 0,188 termasuk ke dalam kriteria sangat rendah. Untuk tingkat kesukaran butir soal terdapat tingkat kesukaran “sangat mudah” berjumlah 17 soal, “mudah” berjumlah 3 soal, “sedang” berjumlah 2 soal, “sukar” berjumlah 1 soal, dan “sangat sukar” berjumlah 1 soal. Pada daya pembeda butir soal, terdapat 14 butir soal tergolong ke dalam kategori “jelek”, 7 butir soal tergolong “cukup”, dan 4 butir soal tergolong “baik”. Sedangkan hasil analisis kualitas pengecoh butir soal, yaitu memiliki tingkat pengecoh yang sangat baik. Selain itu, sangat sedikit pengecoh soal dengan kategori persentase (51-75 atau 126-150) dan 76-125. Oleh karena itu, dapat disimpulkan soal Ujian Tengah Semester Biologi ini belum cukup memenuhi kriteria alat evaluasi pembelajaran dan dapat digunakan untuk mengukur ketercapaian pembelajaran di SMA Negeri 6 Padang.
Specific primer design and optimization of annealing temperature for amplification gene peroxidase (POD) in Oryza sativa L. Nella Fauziah; Afifatul Achyar; Zulyusri Zulyusri; Yusni Atifah; Linda Advinda; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122972

Abstract

Peroksidase (POD) merupakan enzim antioksidan yang memiliki beragam fungsi dalam siklus hidup tanaman, salah satunya adalah sebagai pertahanan dalam melawan ROS dengan mengkatalisis konversi H2O2 menjadi udara dan O2 . Kemampuan aktifitas enzim POD dalam mengatur kandungan H2O2 memungkinkan enzim tersebut dapat mempertahankan tanaman dari cekaman. Metode yang dapat digunakan untuk amplifikasi gen POD salah satunya yaitu kuantitatif reverse transcription- PCR (qRT-PCR). Metode ini memerlukan beberapa komponen penting salah satunya yaitu primer ( forward dan reverse ). Primer yang digunakan dalam amplifikasi gen harus spesifik terhadap gen target sehingga dapat mengenali dan menempel pada gen target yang diinginkan. Penelitian ini bertujuan untuk mendesain primer yang sesuai untuk amplifikasi gen POD menggunakan teknik qRT-PCR. Primer dirancang menggunakan perangkat PrimerQuest. Primer yang telah dirancang kemudian dianalisis untuk spesifikasinya dengan geneious prime . Kemudian spesifikasi primer di cek menggugakan primer BLAST. Hasil desain primer dengan kriteria terbaik untuk amplifikasi gen POD yaitu Forward POD 5'-AAATGCGTCGATCTACTGTACCT-3' dan Reverse POD 5'-GTGTTGAAAATGGCAATAAACCGG-3'
Pengaruh Suhu Terhadap Waktu Fermentasi Nira Aren (Arenga pinnata Merr.) Olan Tri Sinaga; Resti Fevria; Violita Violita; Moralita Chatri
Symbiotic: Journal of Biological Education and Science Vol. 2 No. 1 (2021): Symbiotic: Journal of Biological Education and Science Vol. 2 No. 1 April 2021
Publisher : Institut Agama Islam Negeri Kerinci

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32939/symbiotic.v2i1.12

Abstract

Nira aren merupakan salah satu minuman tradisional yang memiliki khasiat bagi kesehatan. Nira merupakan cairan manis yang diperoleh dengan cara menyadap bunga jantan dari pohon aren. Pohon aren merupakan pohon yang hampir semua bagian fisik maupun produksinya dapat dimanfaatkan dan memiliki nilai ekonomis salah satunya produksi air nira. Nira merupakan cairan manis yang terdapat di dalam bunga tanaman aren yang dimanfaatkan dalam pembuatan gula aren dan tuak, kolang-kaling, ijuk, dan tepung. Nira segar juga digunakan untuk obat sariawan, TBC, disentri, wasir dan memperlancar buang air besar. Penelitian ini bertujuan untuk melihat pengaruh suhu terhadap waktu fermentasi nira aren (Arenga pinnata Merr.). Penelitian ini merupakan penelitian eksperimen menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 6 ulangan dilakukan pada Bulan Desember 2019-Februari 2020 di Laboratorium Penelitian Terpadu Biologi FMIPA UNP. Parameter dari penelitian ini adalah pengukuran pH dan kadar alcohol sebelum fermentasi sampai fermentasi selesai. Data yang didapatkan dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji lanjut DMRT dengan taraf nyata 0,05. Hasil yang didapatkan suhu ruang dengan rata-rata pH 6,88125 dan pada suhu kulkas dengan rata-rata pH 6,924445 serta pada suhu lemari pendingin dengan rata-rata pH 6,893939. Sedangkan kadar alkohol tidak berpengaruh pada setiap perlakuan.
The Validity and Practicality of SSCS-Based Student Worksheet on Ecology Material and Environmental Change Syamsurizal; Irdawati; Violita; Refni Syahleli Afni; Muhamad Sholichin
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4444

Abstract

This research aims to produce student worksheet based on SSCS (Search, Solve, Create and Share). This type of research is development research using the Plomp model. The development stage of this model starts from the initial investigation stage, the development or prototyping stage, and the assessment stage. The instruments used in this development research were teacher questionnaire sheets, student response questionnaire sheets, self-evaluation sheets, and SSCS-based LKPD validation sheets (Search, Solve, Create and Share) by experts, as well as SSCS-based LKPD practicality assessment sheets (Search, Solve, Create and Share) which is filled in by teachers and students. The results showed that the SSCS-based LKPD (Search, Solve, Create and Share) was very valid with a score of 86.31%. The practicality assessment of student assessment in field trials (group evaluation) with a score of 92.00% with very practical criteria, and the practicality assessment by the teacher obtained a score of 96.00% with very practical criteria. It can be concluded that the LKPD based on SSCS (Search, Solve, Create and Share) that has been developed is included in the category of very valid and very practical.
Response of Robusta Coffee (Coffea canephora.L) Germination Stages That Get Treatment with Long Soaking and Gibberellin Concentration (GA3) Nelfia Pitri; violita violita
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Robusta coffee (Coffea canephora L.) is one of the mainstay commodities of plantations in Indonesia. Robusta coffee takes a long time to germinate because Robusta coffee has a hard seed coat so that it is difficult for water and oxygen to penetrate the seed coat which causes the germination process to be hampered. For this reason, it is necessary to speed up germination by stripping the hard seed coat and soaking it with gibberellins. The aim of this study was to obtain the proper concentration of gibberellins and soaking time on the germination of robusta coffee seeds with the husks removed. This research was conducted at the Biology Laboratory, Padang State University in November – January 2022. This study used a completely randomized design (CRD) with two treatment factors. The first factor is the concentration of gibberellins with 3 levels, namely 0, 10, and 20 ppm, the second factor is soaking time with 3 levels, namely 12, 18 and 24 hours. The results showed that the concentration of gibberellins had a significant effect on the parameters of germination height, root length, dry weight, percentage of germination in the early stages of radicle germination and seedling. the duration of immersion and the interaction of the two did not significantly affect the germination rate, vigor index, percentage of germination of seedlings 2 and 3.
The Response Of The Growth Of Robusta Coffee (Coffea Canephora. L) That Received Treatment Of Time Of Immersion And Concentration Of Sulphic Acid (H2SO4) Sri Rahmadani Fitri; Azwir Anhar; Linda Advinda; Violita Violita
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Robusta coffee seeds (Coffea canephora L.) require dormancy breaking treatment to accelerate germination. One of the treatments for breaking dormancy that can be done is chemical scarification by adjusting the concentration and duration of sulfuric acid (H2SO4) immersion. This study aims to determine the best concentration (H2SO4) and soaking time for breaking dormancy of robusta coffee seeds. This research was conducted in the Biology laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University, in November-January 2022 using a two-factor completely randomized design. The first factor is the concentration of H2SO4 solution (0%, 10%, 15% and 20%). The second factor is the immersion time (25 minutes, and 50 minutes). Each treatment combination consisted of 4 replications. Parameters observed were germination percentage, germination rate, vigor index, hypocotyl length (cm), root length (cm), and dry weight (grams). The results showed that the H2SO4 concentration treatment had a very significant effect on the percentage of germination parameters, and dry weight while the other parameters had no significant effect. The treatment duration of immersion had a significant effect on the parameters of germination percentage, germination rate, and dry weight while the other parameters had no significant effect. The combination treatment between H2SO4 concentration and soaking time had a very significant effect on the germination rate parameter, significantly affected the germination percentage parameter, and had no significant effect on other parameters.
The Effectiveness Of Noni Leaf Extract (Morinda citrifolia L.) As Antifungal Against The Growth Of Sclerotium rolfsii In Vitro Marsha Utami; Linda Advinda; Violita Violita; Moralita Chatri
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Pathogens can cause disease in plants such as stem rot, wilting and germination are the fungus Sclerotium rolfsii. S. rolfsii fungus control using synthetic fungicides. However, synthetic fungicides can cause negative impacts such as environmental pollution. Another alternative can be used is a plant-based fungicide. Plants can be used as vegetable fungicides, namely noni leaves (Morinda citrifolia L.) contain several antimicrobial compounds such as flavonoids, alkaloids and steroids that can inhibit fungal growth. The purpose of this study was to test the effectiveness and antifungal activity of noni leaf extract in inhibiting the growth of S. rolfsii. This type of research is an experimental research with 5 treatments and 3 replications which will be carried out in November-December 2021 at the Research Laboratory of the Biology Department, FMIPA UNP. The treatments given were: 0% concentration (control), 10%, 20%, 30% and 40%. The antifungal activity was analyzed descriptively. The results showed that noni leaf extract was effective in inhibiting the growth of the fungus S. rolfsii and all treatments were significantly different from the control. The antifungal activity of noni leaf extract against S. rolfsii at concentrations of 10%, 20% and 30% was weak, while the concentration of 40% was moderate.
The Effect of the Dose of Ecoenzyme on the Increase in Leaf Area of Kangkung Darat (Ipomoea reptans Poir) Yosefin Nisa Aulia; Leilani Eka Putri; Azwir Anhar; Violita Violita
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Ecoenzyme is a processed product from organic waste, water and sugar. Ecoenzyme can be a multifunctional liquid, especially in supporting plant growth. The nutrient content contained in ecoenzyme can encourage vegetative plants including the formation of chlorophyll in leaves. To support growth, various doses of ecoeznyme were given to land kale (Ipomoea reptans Poir). This study aims to determine the effect of the dose of ecoenzyme on the increase in leaf area of ​​land kangkung. This research was carried out from January 2022-February 2022 in the Plant Physiology Laboratory and Biology wire house of FMIPA UNP. This study used a completely randomized design (CRD) with 7 treatments and 4 replications. Consists of: P1 (control), P2 (0.5% ecoenzyme), P3 (1% ecoenzyme), P4 (1.5% ecoenzyme), P5 (2% ecoenzyme), P6 (2.5 ecoenzyme), P7 ( 0.3 POC). The data obtained were analyzed by variance (ANOVA) with DMRT further test. The results showed that the dose of ecoenzyme did not have a significant effect on the increase in leaf area of ​​land kangkung (Ipomoea reptans Poir).
The Influence of Soaking Time and KNO3 Concentration On The vigor indeks of Rice Variety of Cisokan Kuniang (Oryza sativa L.) Expired Al Adawiyah; Violita Violita; Azwir Anhar; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The germination process of expired rice seeds is quite difficult because the seeds have decreased in quality due to expiration. Rice seeds (Oryza sativa L.) expired require increased viability to increase their germination. One way to increase seed viability is to use an invigoration material in the form of KNO3 by adjusting the soaking time and the concentration of KNO3 used. This study aimed to determine the best soaking time and KNO3 concentration to increase the viability of expired rice seeds. This research was conducted at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University in May-June 2022. This study used a completely randomized design (CRD) with two treatment factors. The first factor is the length of immersion with 3 levels, namely 12 hours, 24 hours, and 48 hours. The second factor is the concentration of KNO3 with 5 levels, namely 0%, 1.5%, 2%, 2.5%, and 3%. Each treatment combination consisted of 4 replications. The parameter observed is the vigor index. The results showed that the treatment of KNO3 concentration had a significant effect on the parameters of the vigor index of expired Cisokan kuniang rice varieties. The immersion time had no significant effect on the vigor index parameter. The interaction between immersion time and KNO3 concentration significantly affected the vigor index parameter.
Seed Biopriming Using Plant Growth Promoting Rhizobacteria (PGPR) Milka Saputri; Linda Advinda; Azwir Anhar; Violita; Moralita Chatri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Rhizobacteria are bacteria found on the surface of plant roots and are able to increase plant growth known as Plant Growth Promoting Rhizobacteria (PGPR). Rhizobakteri can be used for plant germination by biopriming. Biopriming is hydration of seeds in a controlled manner using biological compounds. Several species of plant growth promoting rhizobacteria that can be used for biopriming are Pseudomonas fluorescens, P. putida and Bacillus subtilis. Biopriming is an alternative method for controlling seeds from soil-borne pathogens and can help farmers use microbes to apply the crops they want. From several research results, biopriming has been proven to increase plant productivity.
Co-Authors Abdul Razak Achyar, Afifatul Ahmad Subari Al Adawiyah Anggara Dwi Putra Zagoto Atifah, Yusni Ayu Adelia Azwir Anhar Celsi Ananda Des Des M Dezi Handayani Dinda Sahara Dwi Hilda Putri Elsa Sri Handayani Eria Marina Sepriyani Fevria, Resti Fika Faradhila Helsa Rahmatika Heron Tarigan Irdawati Irdawati Irma Leilani Eka Putri Jalilah Azizah Jumatul Hafsah Leilani Eka Putri Linda Advinda Lufri Lufri Marsha Utami Milka Saputri Moralita Chatri Muhamad Sholichin Nanta Mulia Nelfia Pitri Nella Fauziah Nofi Yendri Sudiar Nurhelmi Nurhelmi Nurul Rahmi Olan Tri Sinaga Putri Widianti Ratih Rahayu Refni Syahleli Afni Resti Pevria Restu Fitriani Rijal Satria Sintia Putri Sri Rahmadani Fitri Syamsurizal S Syifa Fajrisani Tomi Apra Santosa Santosa Yolanda Karinia Yosefin Nisa Aulia Yulkifli Yulkifli Zulyusri, Zulyusri