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Fungi Isolated from litter’s forest of Aka Barayun, Lembah Harau and It’s Phosphate Solubilizing Activity Nurul Rahmi; Dezi Handayani; Dwi Hilda Putri; Violita Violita
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

One of the essential nutrients for plant growth is phosphate, but just 0.1-0.5% is available for plants to use. This problem can be solved by using chemical fertilizers, but for long run could damages environment. An alternative way for chemical fertilizers is using phosphate solubilizing fungi. Phosphate-solubilizing fungi can be isolated from soil or litter. This study aims to isolated fungi from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra and determine it’s phosphate solubilizing potency. Medium for isolation was Potato Dextrose Agar (PDA) and medium for phosphate solubilizing activity test was solid Pikovskaya medium with source of bound phosphate Ca3(PO4)2. Thirteen fungi were isolated successfully from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra, which are coded SR1-SR13. Based on macroscopic and microscopic morphology, it is known that two isolates belong to the genus Penicillium, two isolates belong to Trichoderma, one isolate of Verticillium, one isolate of Fusarium, two isolates of yeast and two isolates are not yet known. Results of the phosphate solubilizing test showed that only seven isolates were able to solubilize phosphate, they are isolates SR2, SR5, SR6, SR7, SR11, SR 12 and SR 13. Phosphate solubilization index is low and ranges from 0.1 to 0.4.
Potency of Yeast from Orange Peel’s Ecoenzyme as Antimicrobe Elsa Sri Handayani; Dezi Handayani; Irdawati Irdawati; Violota Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Orange peels can still be utilized even though they are categorized as waste. Orange peels still have quite a lot of nutrients and contain various active compounds. Orange peels can be used as a source of organic material for the manufacture of ecoenzyme. The ecoenzyme produced from orange peel has a distinctive and fragrant aroma so that it is preferred compared to ecoenzyme made from other organic materials. During the ecoenzyme fermentation process, there are various types of bacteria and fungi that grow, but their roles are not yet known. The results of previous research obtained eight yeast isolates from the fermentation process of orange peel ecoenzyme, but only six isolates can be tested because two isolates are contaminated. The purpose of this study was to see the potential of yeast from citrus peel ecoenzyme as an antimicrobial. The antimicrobial activity test method used was the agar solid diffusion test. The presence of antimicrobial activity is characterized by a zone of inhibition around the colony of yeast isolates. The results showed that all isolates of yeast from orange peels ecoenzyme were able to inhibit the growth of E. coli and only five isolates were able to inhibit the growth of S. aureus, but none were able to inhibit C. albicans. The diameter of the inhibition zone against E. coli ranged from 8.3 mm to 17.5 mm while S. aureus ranged from 7.8 mm to 12.9 mm. Therefore, yeast derived from orange peel ecoenzyme has potential as an antimicrobial, especially against E. coli. and S. aureus.
Isolasi Cendawan Endofit Pelarut Fosfat dari Akar Tumbuhan Pakis Simpei (Cibotium barometz(L) J.Sm) Celsi Ananda; Dezi Handayani; Azwir Anhar; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Cendawan pelarut fosfat merupakan cendawan yang mampu mengubah fosfat yang tidak larut menjadi fosfat bebas, Sehingga dapat diserap oleh tanaman dengan mudah. Cendawan pelarut fosfat diisolasi dari tanah atau rizosfer, atau cendawan endofit. Cendawan endofit dapat ditemukan pada akar, batang atau daun tumbuhan. Akar tanaman merupakan habitat yang baik bagi cendawan pelarut fosfat, antara lain akar tumbuhan pakis simpei (Cibotium barometz (L) J.Sm). Tujuan dari penelitian ini adalah untuk mengisolasi cendawan endofit dari akar tumbuhan pakis simpei, dan untuk mengetahui kemampuan melarutkan fosfatnya. Penelitian dilaksanakan di Laboratorium Biologi Umum FMIPA UNP pada bulan Januari hingga Maret 2023. Sampel akar tumbuhan pakis simpei berasal dari Lembah Harau, Kecamatan Harau, Kabupaten Lima Puluh Kota, Sumatera Barat. Potato Dextrose Agar (PDA) digunakan sebagai media isolasi dan Pikovskaya sebagai uji pelarut fosfat. Sterilisasi permukaan menggunakan NaOCl 0,5%. Hasil penelitian menunjukkan bahwa 6 isolat murni cendawan berhasil diisolasi. Terdapat dua isolat yang memiliki aktivitas pelarut fosfat yaitu AK5 dan AK6. Indeks kelarutan terbesar diperoleh pada hari ketiga untuk isolat AK5 dan AK6. Indeks kelarutan adalah 0,2 dan tergolong kategori lemah.
Specific Primer Design and Optimization of Monodehydroascorbate reductase (MDHAR) Gene Amplification in Rice (Oryza sativa L.) Jumatul Hafsah; Afifatul Achyar; Zulyusri; Yusni Atifah; Linda Advinda; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Monodehydroascorbate reductase (MDHAR) is an enzyme responsible for growth and response to biotic and abiotic stress. MDHAR in rice shows a higher sensitivity to stress compared to other plants. This study aims to obtain specific primers for the MDHAR gene in rice to be used in PCR amplification so that it can amplify the MDHAR gene. Primers are designed using the Pickprimer and Geneious Primer tools. Optimization of annealing temperature was carried out using the gradient PCR method and then an in vitro primary specification test was carried out using the Touchdown PCR method. The results of the primary design obtained one candidate primer that met the ideal primer requirements, namely a pair of primers (5'-AAAAACACTGCATGGGTCGTC-3' and 5'-CGCCTACCGTTTCCCAAGTT-3') with an amplicon length of 160 bp. The visualization results of PCR products using 1.5% agarose showed that 6 samples were able to amplify the MDHAR gene at 160 bp in size. However, in each lane there is a non-specific DNA band (Primer dimer). In vitro primer specification testing with Touchdown succeeded in increasing product formation specifications and was able to reduce non-specific DNA bands (Primer dimers).
Analisis E-Learning Dalam Pembelajaran Evolusi Mahasiswa Pendidikan Biologi Selama Pandemi Covid-19 Tomi Apra Santosa; Eria Marina Sepriyani; Lufri Lufri; abdul razak; Moralita Chatri; Violita Violita
Edumaspul: Jurnal Pendidikan Vol 5 No 1 (2021): Edumaspul: Jurnal Pendidikan
Publisher : Universitas Muhammadiyah Enrekang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33487/edumaspul.v5i1.1027

Abstract

Penelitian ini bertujuan untuk mengetahui analisis e-learning dalam pembelajaran evolusi mahasiswa pendidikan biologi selama pandemi covid-19. Penelitian ini adalah penelitian studi pustaka (library research). Sumber data berasal dari jurnal nasional dan internasional bereputasi, buku dan sumber relevan lainnya. Teknik pengumpulan data dengan menelusuri jurnal dan buku yang berkaitan dengan kemampuan metakognitif mahasiswa. Hasil penelitian ini disimpulkan bahwa pembelajaran e-learning selama Covid-19 berjalan dengan efektif dalam pembelajaran evolusi dan memberikan kemudahan kepada mahasiswa dalam memahami materi pelajaran.
EDUKASI JAJANAN SEHAT KEPADA SISWA SD DALAM MENGHADAPI ERA NEW NORMAL Yusni Atifah; Afifatul Achyar; Rijal Satria; Violita Violita; Helsa Rahmatika; Jalilah Azizah
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 1 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v6i1.213-217

Abstract

Anak usia sekolah memerlukan asupan energi dan zat gizi untuk mendukung pertumbuhan dan juga prestasi belajar anak di sekolah. Salah satu sumber asupan energi dan zat gizi bagi anak sekolah bersumber dari pangan jajanan. Anak usia sekolah sebagai generasi penerus bangsa merupakan investasi bagi suatu bangsa. Kualitas anak-anak pada masa sekarang akan menentukan kualitas suatu negara di masa depan. Edukasi keamanan pangan jajanan perlu dilakukan sejak dini secara sistematis dan juga berkelanjutan sebagai salah satu upaya untuk peningkatan kualitas sumber daya manusia. Pemberian nutrisi yang berkualitas, sehat dan sesuai dengan kuantitas yang seharusnya penting untuk menunjang pertumbuhan perkembangan anak usia sekolah agar optimal. Oleh karena itu, perlu adanya edukasi kepada siswa SD untuk meningkatkan pengetahuan terkait keamanan pangan jajanan dan mengetahui arti simbol-simbol pada bungkus jajanan. Kegiatan ini dilaksanakan pada bulan Juli 2022, dan diikuti oleh 206 peserta dari kelas 3 dan 6 SDN 02 Payakumbuh. Hasil evaluasi kegiatan menunjukkan adanya peningkatan pengetahuan siswa terhadap keamananan jajanan dan memiliki keterampilan dalam mengidentifikasi simbol-simbol pada bungkus jajanan. Kesimpulan untuk kegiatan ini sudah efektif dilaksanakan dan dapat mengedukasi siswa SD dalam  mengetahui keamanan pangan jajanan.
The Validity and Practicality of SSCS-Based Student Worksheet on Ecology Material and Environmental Change Syamsurizal; Irdawati; Violita; Refni Syahleli Afni; Muhamad Sholichin
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4444

Abstract

This research aims to produce student worksheet based on SSCS (Search, Solve, Create and Share). This type of research is development research using the Plomp model. The development stage of this model starts from the initial investigation stage, the development or prototyping stage, and the assessment stage. The instruments used in this development research were teacher questionnaire sheets, student response questionnaire sheets, self-evaluation sheets, and SSCS-based LKPD validation sheets (Search, Solve, Create and Share) by experts, as well as SSCS-based LKPD practicality assessment sheets (Search, Solve, Create and Share) which is filled in by teachers and students. The results showed that the SSCS-based LKPD (Search, Solve, Create and Share) was very valid with a score of 86.31%. The practicality assessment of student assessment in field trials (group evaluation) with a score of 92.00% with very practical criteria, and the practicality assessment by the teacher obtained a score of 96.00% with very practical criteria. It can be concluded that the LKPD based on SSCS (Search, Solve, Create and Share) that has been developed is included in the category of very valid and very practical.
The Development of LKPD Based on a Scientific Approach to Improve Students' Science Process Skills in Material on the Human Circulatory System and Digestive System Class XI SMA Mia Ramayani; Violita; Abdul Razak; Muhyiatul Fadilah
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.7705

Abstract

The development of Student Worksheets (LKPD) based on a scientific approach to material on the human circulation system and digestive system is based on the teaching materials used at SMA Negeri 16 Padang in the form of Student Worksheets (LKPD). The LKPD is not complete in terms of components or in terms of LKPD elements. The LKPD used only takes the form of sheets containing questions, and does not use an approach that contains activities or learning steps so that it cannot train students' science process skills. This research aims to produce LKPD based on a scientific approach to material on the human circulation system and digestive system that is valid, practical and effective to improve students' science process skills. This research is development research using the Plomp model which consists of three stages, namely preliminary research phase, development or prototyping phase and assessment phase.   The results of the LKPD validity test by the validator obtained a value of 84.67% with very valid criteria.   The results of the LKPD practicality test obtained a score of 90.74% by teachers and 88.30% by students with very practical criteria. The results of the T-test showed differences in values between the experimental and control classes with a significance of 0.000 each in the circulatory system and digestive system material. It can be concluded that LKPD based on a scientific approach is very valid, very practical and effective in improving science process skills.
Teknik Pembibitan Tanaman Kemiri (Aleurites moluccana L.) Di UPTD BSPTH Dinas Kehutanan Provinsi Sumatera Barat Annisa Fauziah; Violita Violita; Mika Lestaria
Jurnal Sains Dan Teknologi | E-ISSN : 3063-9980 Vol. 2 No. 4 (2026): April - Juni
Publisher : GLOBAL SCIENTS PUBLISHER

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Abstract

A professional internship was conducted at the Forest Plant Certification and Seedling Center (UPTD BSPTH) of West Sumatra Provincial Forestry Service from January 5 to February 18, 2026, focusing on the seedling techniques of candlenut (Aleurites moluccana L.). This article aims to describe the seedling stages applied at UPTD BSPTH, including seed selection, pre-germination treatment, sowing, germination observation, transplanting to polybags, and seedling maintenance. The method used was participative observation and direct field observation. Results showed that the nursery medium consisted of a mixture of mineral soil, fine sand, organic compost, and rice husks. Mechanical scarification treatment was effective in increasing germination percentage. Seeds germinating in the center of the seedbed tended to sprout earlier due to more even moisture and temperature distribution. The produced candlenut seedlings showed good quality, characterized by strong taproots, erect dark-green stems, and normally developed leaves. The seedling technique applied at UPTD BSPTH is in accordance with the principles of forest plant nursery management, producing seedlings suitable for land rehabilitation and reforestation programs in West Sumatra.