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All Journal Bioscience Jurnal Pendidikan Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Martabe : Jurnal Pengabdian Kepada Masyarakat Eksakta : Berkala Ilmiah Bidang MIPA Pelita Eksakta Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi Edumaspul: Jurnal Pendidikan
Violita Violita
Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Social Sciences Transportation Other

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Fungi Isolated from litter’s forest of Aka Barayun, Lembah Harau and It’s Phosphate Solubilizing Activity Nurul Rahmi; Dezi Handayani; Dwi Hilda Putri; Violita Violita
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

One of the essential nutrients for plant growth is phosphate, but just 0.1-0.5% is available for plants to use. This problem can be solved by using chemical fertilizers, but for long run could damages environment. An alternative way for chemical fertilizers is using phosphate solubilizing fungi. Phosphate-solubilizing fungi can be isolated from soil or litter. This study aims to isolated fungi from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra and determine it’s phosphate solubilizing potency. Medium for isolation was Potato Dextrose Agar (PDA) and medium for phosphate solubilizing activity test was solid Pikovskaya medium with source of bound phosphate Ca3(PO4)2. Thirteen fungi were isolated successfully from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra, which are coded SR1-SR13. Based on macroscopic and microscopic morphology, it is known that two isolates belong to the genus Penicillium, two isolates belong to Trichoderma, one isolate of Verticillium, one isolate of Fusarium, two isolates of yeast and two isolates are not yet known. Results of the phosphate solubilizing test showed that only seven isolates were able to solubilize phosphate, they are isolates SR2, SR5, SR6, SR7, SR11, SR 12 and SR 13. Phosphate solubilization index is low and ranges from 0.1 to 0.4.
Potency of Yeast from Orange Peel’s Ecoenzyme as Antimicrobe Elsa Sri Handayani; Dezi Handayani; Irdawati Irdawati; Violota Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Orange peels can still be utilized even though they are categorized as waste. Orange peels still have quite a lot of nutrients and contain various active compounds. Orange peels can be used as a source of organic material for the manufacture of ecoenzyme. The ecoenzyme produced from orange peel has a distinctive and fragrant aroma so that it is preferred compared to ecoenzyme made from other organic materials. During the ecoenzyme fermentation process, there are various types of bacteria and fungi that grow, but their roles are not yet known. The results of previous research obtained eight yeast isolates from the fermentation process of orange peel ecoenzyme, but only six isolates can be tested because two isolates are contaminated. The purpose of this study was to see the potential of yeast from citrus peel ecoenzyme as an antimicrobial. The antimicrobial activity test method used was the agar solid diffusion test. The presence of antimicrobial activity is characterized by a zone of inhibition around the colony of yeast isolates. The results showed that all isolates of yeast from orange peels ecoenzyme were able to inhibit the growth of E. coli and only five isolates were able to inhibit the growth of S. aureus, but none were able to inhibit C. albicans. The diameter of the inhibition zone against E. coli ranged from 8.3 mm to 17.5 mm while S. aureus ranged from 7.8 mm to 12.9 mm. Therefore, yeast derived from orange peel ecoenzyme has potential as an antimicrobial, especially against E. coli. and S. aureus.
Isolasi Cendawan Endofit Pelarut Fosfat dari Akar Tumbuhan Pakis Simpei (Cibotium barometz(L) J.Sm) Celsi Ananda; Dezi Handayani; Azwir Anhar; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Cendawan pelarut fosfat merupakan cendawan yang mampu mengubah fosfat yang tidak larut menjadi fosfat bebas, Sehingga dapat diserap oleh tanaman dengan mudah. Cendawan pelarut fosfat diisolasi dari tanah atau rizosfer, atau cendawan endofit. Cendawan endofit dapat ditemukan pada akar, batang atau daun tumbuhan. Akar tanaman merupakan habitat yang baik bagi cendawan pelarut fosfat, antara lain akar tumbuhan pakis simpei (Cibotium barometz (L) J.Sm). Tujuan dari penelitian ini adalah untuk mengisolasi cendawan endofit dari akar tumbuhan pakis simpei, dan untuk mengetahui kemampuan melarutkan fosfatnya. Penelitian dilaksanakan di Laboratorium Biologi Umum FMIPA UNP pada bulan Januari hingga Maret 2023. Sampel akar tumbuhan pakis simpei berasal dari Lembah Harau, Kecamatan Harau, Kabupaten Lima Puluh Kota, Sumatera Barat. Potato Dextrose Agar (PDA) digunakan sebagai media isolasi dan Pikovskaya sebagai uji pelarut fosfat. Sterilisasi permukaan menggunakan NaOCl 0,5%. Hasil penelitian menunjukkan bahwa 6 isolat murni cendawan berhasil diisolasi. Terdapat dua isolat yang memiliki aktivitas pelarut fosfat yaitu AK5 dan AK6. Indeks kelarutan terbesar diperoleh pada hari ketiga untuk isolat AK5 dan AK6. Indeks kelarutan adalah 0,2 dan tergolong kategori lemah.
Specific Primer Design and Optimization of Monodehydroascorbate reductase (MDHAR) Gene Amplification in Rice (Oryza sativa L.) Jumatul Hafsah; Afifatul Achyar; Zulyusri; Yusni Atifah; Linda Advinda; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Monodehydroascorbate reductase (MDHAR) is an enzyme responsible for growth and response to biotic and abiotic stress. MDHAR in rice shows a higher sensitivity to stress compared to other plants. This study aims to obtain specific primers for the MDHAR gene in rice to be used in PCR amplification so that it can amplify the MDHAR gene. Primers are designed using the Pickprimer and Geneious Primer tools. Optimization of annealing temperature was carried out using the gradient PCR method and then an in vitro primary specification test was carried out using the Touchdown PCR method. The results of the primary design obtained one candidate primer that met the ideal primer requirements, namely a pair of primers (5'-AAAAACACTGCATGGGTCGTC-3' and 5'-CGCCTACCGTTTCCCAAGTT-3') with an amplicon length of 160 bp. The visualization results of PCR products using 1.5% agarose showed that 6 samples were able to amplify the MDHAR gene at 160 bp in size. However, in each lane there is a non-specific DNA band (Primer dimer). In vitro primer specification testing with Touchdown succeeded in increasing product formation specifications and was able to reduce non-specific DNA bands (Primer dimers).
Analisis E-Learning Dalam Pembelajaran Evolusi Mahasiswa Pendidikan Biologi Selama Pandemi Covid-19 Tomi Apra Santosa; Eria Marina Sepriyani; Lufri Lufri; abdul razak; Moralita Chatri; Violita Violita
Edumaspul: Jurnal Pendidikan Vol 5 No 1 (2021): Edumaspul: Jurnal Pendidikan
Publisher : Universitas Muhammadiyah Enrekang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33487/edumaspul.v5i1.1027

Abstract

Penelitian ini bertujuan untuk mengetahui analisis e-learning dalam pembelajaran evolusi mahasiswa pendidikan biologi selama pandemi covid-19. Penelitian ini adalah penelitian studi pustaka (library research). Sumber data berasal dari jurnal nasional dan internasional bereputasi, buku dan sumber relevan lainnya. Teknik pengumpulan data dengan menelusuri jurnal dan buku yang berkaitan dengan kemampuan metakognitif mahasiswa. Hasil penelitian ini disimpulkan bahwa pembelajaran e-learning selama Covid-19 berjalan dengan efektif dalam pembelajaran evolusi dan memberikan kemudahan kepada mahasiswa dalam memahami materi pelajaran.
Co-Authors Abdul Razak Achyar, Afifatul Ahmad Subari Al Adawiyah Anggara Dwi Putra Zagoto Atifah, Yusni Ayu Adelia Azwir Anhar Celsi Ananda Des Des M Dezi Handayani Dinda Sahara Dwi Hilda Putri Elsa Sri Handayani Eria Marina Sepriyani Fevria, Resti Fika Faradhila Helsa Rahmatika Heron Tarigan Irdawati Irdawati Irma Leilani Eka Putri Jalilah Azizah Jumatul Hafsah Leilani Eka Putri Linda Advinda Lufri Lufri Marsha Utami Milka Saputri Moralita Chatri Muhamad Sholichin Nanta Mulia Nelfia Pitri Nella Fauziah Nofi Yendri Sudiar Nurhelmi Nurhelmi Nurul Rahmi Olan Tri Sinaga Putri Widianti Ratih Rahayu Refni Syahleli Afni Resti Pevria Restu Fitriani Rijal Satria Sintia Putri Sri Rahmadani Fitri Syamsurizal S Syifa Fajrisani Tomi Apra Santosa Santosa Yolanda Karinia Yosefin Nisa Aulia Yulkifli Yulkifli Zulyusri, Zulyusri