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Literature Review: The Role of Ascorbic Acid as a Priming Compound in the Physiological Response of Plants to Drought Stress Muftia Nadhra; Anggraini Anggraini; Violita Violita
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11463

Abstract

Drought stress is a major abiotic factor that limits plant growth and productivity by disrupting photosynthesis, water uptake, and cellular metabolism. One strategy to enhance plant tolerance to drought is seed priming using ascorbic acid (AsA), which functions as an important antioxidant in plant defense systems. This study aims to systematically review the role of AsA as a priming compound in improving plant physiological responses under drought stress. A systematic literature review was conducted following the PRISMA framework. Relevant articles were collected from Google Scholar, ScienceDirect, and MDPI databases using keywords related to drought stress, seed priming, and ascorbic acid. Selected studies were analyzed descriptively to identify patterns of plant responses. The results indicate that AsA priming enhances antioxidant enzyme activities, reduces reactive oxygen species accumulation, maintains membrane stability, and improves germination, photosynthesis, and early seedling growth across several crop species. In conclusion, AsA seed priming is an effective approach to improve plant tolerance to drought stress. Future studies are recommended to explore optimal concentrations and molecular mechanisms of AsA application in different crop species.
Pengaruh Priming Auksin Terhadap Perkecambahan Benih Padi Ir64 Pada Cekaman Salinitas Anggraini Anggraini; Muftia Nadhra; Yoana Marizky Siregar; Violita Violita
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11474

Abstract

Salinity is a major abiotic constraint that limits rice establishment, particularly during germination when seedlings are physiologically vulnerable. This study investigated the effect of auxin priming on germination performance and early seedling growth of IR64 rice exposed to 100 mM NaCl, and identified the most effective auxin concentration. The experiment was conducted using a Completely Randomized Design with five treatments: non-primed seeds and auxin priming at concentrations of 0, 25, 50, and 100 ppm, each with three replications. Statistical analysis was performed using analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test at the 5% significance level. Parameters observed included germination percentage and rate, root and plumule length, total seedling length, vigor index, fresh and dry weight, and lipid peroxidation. Auxin priming significantly enhanced germination rate, seedling growth, vigor index, and biomass accumulation, while reducing lipid peroxidation under saline conditions. Among the treatments, P3 exhibited the most consistent improvement under salinity stress, whereas P1 showed the highest overall growth under non-stress conditions. These findings indicate that appropriate auxin priming can improve the early physiological performance of rice seedlings under salt stress.
Analysis of Genetic Variations in bHLH Protein (Rc) Gene Sequences in Rice (Oryza) NCBI PopSet 2496581476 Using In-Silico RFLP: Analisis Variasi Genetik Sekuen Gen Rc-bHLH Protein pada Padi (Oryza) NCBI PopSet 24596581476 Menggunakan RFLP secara In-Silico Nurul Hasanah; Violita Violita; Afifatul Achyar
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.50

Abstract

Rice is a plant from the Graminae Family that is spread almost throughout the region. In rice, there is an Rc gene that codes for the formation of the basic loop-helix-loop (bHLH) protein, the Rc gene (Rc-bHLH) functions as a transcription of color pigments in brown rice. This research was conducted computationally from several related sites. The study used restriction enzyme BstUI with a side recognition of 5'-CG'CG-3'. The purpose of this study was to analyze the genetic variation of the Rc gene sequence encoding the bHLH protein in NCBI PopSet rice 2496581476 using RFLP in silico. The results showed that there were genetic variations of the Rc-bHLH gene, 2 allele variations were present in 21 rice sequences using the restriction enzyme BstUI.
Chemical Control of Coffee Leaf Diseases in an Integrated Pest Management System: A Literature Review Maidatul Isnaini; Violita Violita
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12928

Abstract

Coffee leaf diseases, particularly coffee leaf rust caused by Hemileia vastatrix, remain one of the major constraints to coffee production in Indonesia and other coffee-producing countries, causing yield losses of up to 50% under severe infection. Synthetic fungicides are still the primary control strategy because of their rapid and reliable disease suppression. This review aimed to evaluate the effectiveness of synthetic fungicides in managing coffee leaf diseases, compare the performance of different fungicide groups, and identify recent trends in their application. A literature review was conducted using peer-reviewed articles published between 2015 and 2025 retrieved from Scopus, Web of Science, ScienceDirect, and Google Scholar. The search employed the keywords “coffee leaf rust,” “Hemileia vastatrix,” “synthetic fungicide,” “triazole,” “strobilurin,” and “coffee disease management.” Only English- and Indonesian-language articles reporting fungicide efficacy under field or greenhouse conditions were included. The reviewed studies consistently showed that triazole- and strobilurin-based fungicides were the most effective, generally reducing coffee leaf rust severity by 60–90% when applied at the early stages of infection. However, repeated application of single-mode-of-action fungicides increases the risk of pathogen resistance and environmental contamination. This review highlights the comparative effectiveness of major synthetic fungicide groups and emphasizes that their use should be integrated with resistant cultivars, cultural practices, and fungicide rotation within an Integrated Pest Management (IPM) framework to support sustainable coffee production. Further studies are recommended to optimize fungicide rotation strategies and evaluate environmentally safer alternatives.