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PEMBINAAN PEDAGANG MAKANAN KAKI LIMA UNTUK MENINGKATKAN HIGIENE DAN SANITASI PENGOLAHAN DAN PENYEDIAAN MAKANAN DI DESA PENATIH, DENPASAR TIMUR I Nengah Sujaya; Ni Made Utami Dwipayanti; Ni Ketut Sutiari; L.P Lila Wulandari; Ni Kadek Tresna Adhi
Buletin Udayana Mengabdi Vol 9 No 1 (2010): Volume 9 No.1 – April 2010
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Abstract Generally street vendor has little knowledge on hygiene and sanitation of food handling. Usually they trade in a night market or on street sides. There are two markets in Penatih Village that operate night market. Both markets located in a strategic location thus they have many visitors every day. In these two markets there are some vendors that need to be counseled and assisted in order to improve their knowledge on hygiene and sanitation of food handling. The counseling and assistance was conducted every week from 25 September to 10 October 2009, with 10 vendors as target participants. The venues were located in Penatih Market and Agung Market, Penatih Village, East Denpasar. The activities were conducted in the form of counseling and assisting during trading hours towards the participants in regards of hygiene and sanitation of food handling. In order to evaluate and measure the knowledge improvement of the participants, pre test and post test were carried out. The result shows that, most of participants have less knowledge on food additives in terms of usage, types and risk of usage. The participants have good knowledge on usage of clean water for utensil and food washing as well as knowledge on personal hygiene. It is recommended to conduct a continuous and regular counseling to street vendors with the involvement of local primary health care (puskesmas).
Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM SUMBAWA MARE MILK Nengah Sujaya; Yan Ramona; Ni Putu Widarini; Ni Putu Suariani; Ni Made Utama Dwipayanti; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to isolate and characterize lactic acid bacteria (LAB) from the Sumbawa mares milk The Isolation of LAB was conducted in Man Rogosa Sharpe (MRS) agar. The isolates were characterized by standard methods, such as Gram staining, cell morphology study and fermentation activities. The ability of the isolates to inhibit some pathogenic bacteria was studied by dual culture assay. Isolates showing the widest spectrum of inhibiting pathogenic bacteria were further identified using API 50 CHL. The results showed that Sumbawa mare milk was dominated by lactobacilli and weisella/leuconostoc. As many as 26 out 36 isolates belong to homofermentative lactobacilli and another 10 isolates belong to both heterofermentative lactobacilli and weissella or leuconostoc. Twenty four isolates inhibited the growth of Escherichia coli 25922, Shigela flexneri, Salmonella typhimurium, and Staphylococcus aureus 29213. Two promising isolates with the widest spectrum of inhibiting pathogenic bacteria, Lactobacillus sp. SKG34 and Lactobacillus sp. SKG49, were identified respectively as Lactobacillus rhamnosus SKG34 and Lactobacillus ramnosus SKG49. These two isolates were specific strains of the sumbawa mare milk and are very potential to be developed as probiotic for human.
PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK I Nengah Sujaya; I Made Utami Dwipayanti; Ni Luh Putu Suariani; Ni Putu Widarini; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this capability is not appropriate for probiotic. This character is worthly to be studied since it has never been reported in lactobacilli.
KUALITAS MIKROBIOLOGI NASI JINGGO BERDASARKAN ANGKA LEMPENG TOTAL, COLIFORM TOTAL DAN KANDUNGAN ESCHERICHIA COLI Ni Luh Payastiti Yunita; Ni Made Utami Dwipayanti
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Inadequate quality of food products that do not meet standard quality may lead to food poisoning cases and become a concern in public health sector. Nasi Jinggo is a small amount of cooked rice with little bit fried tempe, shredded spicy chicken, traditional made chili sauce that is packed with banana leaves, and all the cooking process usually done traditionally. Nasi jinggo is sold at night by street vendor with price ranged from Rp. 1.500,- to Rp. 2.000,- per package. The study was a quantitative descriptive study with cross-sectional design that was aimed to identify the microbiology quality of nasi jinggo that were sold in Denpasar Selatan area. From 48 population of nasi jinggo street vendor, 23 vendor was taken as samples. The microbiology quality being assessed was Total Plate Count (TPC), Total Coliform with MPN method and Escherichia coli content with Eosin Methylene Blue Agar as medium. Laboratory analysis showed that only 21,7% sample that met TPC standard with average value of 2,3 x 107 CFU/gram sample, only 8,7% that met Total Coliform standard with average value of 1,4 x 105 MPN/100 gram sample and 52,2% sample that met E. coli content standard with average value is 2,6 x 102 MPN/100 gram sample. It is recommended to the government to do quality check towards nasi jinggo in a regular basis as well as to provide education and assistance to nasi jinggo street vendors in order to improve the microbiology quality of food product and ensure food safety to the consumer.
SUSTAINABLE BENCHMARKS FOR TOURIST ACCOMMODATIONS IN BALI Ni Made Utami Dwipayanti
Bumi Lestari Journal of Environment Vol 6 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

As part of our efforts to move towards sustainable development, it is very importantto encourage the tourism industry to be more environmentally conscious because itconsumes large amounts of resources. One way of achieving greater sensitivity is to identifybenchmarks for energy and water use and for other aspects of hotel operations like theamount of solid waste generated. Benchmarks can be defined using data from existinghotels. The research project focused on tourist accommodation on Southern Part of BaliIsland. It conducted a survey by mailed questionnaire of about 50% of the hotels on theisland. The questionnaire obtained annual data on several sustainability criteria that areenergy and water consumption, biodiversity conservation, resource conservation, cleaningchemical use, solid waste management and wastewater management. The data were usedto derive baseline and best practice thresholds to improve the performance of touristaccommodation on Bali Island.The benchmarks, both baseline and best practice standards were developed mainlyusing the study results which established the average and the average of the top 25% ofperformers. The average of the top 10% of performers was also considered in selecting thebest practice standard for indicators that required stricter standards. Within thebenchmarking process, Green Globe 21 standards and some other studies were also beingused for comparison purpose.In this survey, the difficulties were found in obtaining complete set of data from eachrespondents as well as gaining sufficient responses. Few hotels provided complete set ofdata. Data on energy and water consumption is the easiest to obtain, while data for otherindicators only provided by less than 75% of respondents. Most of the survey resultsshowed reasonable value compared to Green Globe 21 standards. Good performance oftourist accommodation in the survey was found in energy consumption indicator that theaverage and the average of the top 25% of performers were far stricter than the Green Globe21 standards. The study concluded with benchmarks recommendation for each indicatorthat can be applied to tourist accommodations in Southern Part of Bali Island.
Health Risks Assessment of Heavy Metal from Consumption of Oreochromis Mossambicus and Oreochromis Niloticus in Denpasar, Bali Ni Made Utami Dwipayanti; I Gst. Ayu Kunti Sri Panca Dewi; Ni Putu Gita Saraswati Palgunadi; Muliana Rofida; Ni Ketut Sutiari; I Gede Herry Purnama
JURNAL KESEHATAN LINGKUNGAN Vol. 13 No. 4 (2021): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v13i4.2021.250-258

Abstract

Introduction: Heavy metals in the environment can accumulate in organisms through the food chain process. Previous studies recorded heavy metal concentrations above threshold limits value in Badung river, which warrants monitoring adverse health outcomes due to consuming fish from this river. This research aimed to estimate the potential risk from heavy metals exposure, namely Pb, Cd, Cu, and Cr, due to fish consumption. Methods: Fish samples were limited to Oreochromis Mossambicus and Oreochromis niloticus, commonly known as tilapia. Twenty samples of composite fish muscles were collected from three fishing sites. Subsequently, the heavy metals present in the samples were quantified using an atomic absorption spectrophotometer (AAS), and health risks were assessed by calculating estimated daily intake (EDI) and target hazard quotient (THQ) for both carcinogenic and non-carcinogenic risks. Results and Discussion: The average concentrations of Pb in Oreochromis Mossambicus (6.35±3.21 mg/kg) and Oreochromis niloticus (6.09±3.07 mg/kg) exceed the threshold limits value for fish products, but other heavy metals remain below. The average EDI from consuming Oreochromis Mossambicus with Pb is 0.0025-0.0026 mg/kg/days; Cu is 0.0037- 0.0062 mg/kg/day; Cr is 0.0001 mg/kg/day. EDI from consuming Oreochromis niloticus with Pb is 0.0015-0.0025 mg/kg/day; and 0.00 mg/kg/day for Cu and Cr. The THQ calculation for carcinogenic and non-carcinogenic health risks showed no health risk from consuming the fish from the Badung River. Conclusion: The study concludes that the consumption of Oreochromis Mossambicus and Oreochromis niloticus from the Badung River was generally safe from potential health risks.
Analisis faktor risiko infeksi malaria sesudah bencana gempa bumi di Kabupaten Lombok Barat, Indonesia Michelle Amazing Grace Rampengan; Ni Made Utami Dwipayanti; Putu Cintya Denny Yuliyatni
Intisari Sains Medis Vol. 11 No. 1 (2020): (Available online: 1 April 2020)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.084 KB) | DOI: 10.15562/ism.v11i1.660

Abstract

Background: Malaria is an infectious disease that often arises in post-disaster situations such as earthquakes. The earthquake that occurred in Lombok caused damage to people's homes and forced them to live in emergency tents. This condition increases the risk of malaria transmission so that malaria outbreak occurs. This study aims to determine the risk factors for malaria infection after the earthquake disaster in West Lombok Regency.Methods: A case-control study was conducted among 168 respondents located in four Public Health Services working areas in West Lombok. Cases were positive malaria sufferers based on microscopic examination results and were recorded in the health centre register in the September-November 2018 period. In addition, the controls were people who did not suffer from malaria based on microscopic examination results and lived in the study area. Case samples were selected by systematic random sampling from the register of health centres and control samples were selected by convenient sampling from communities living in one hamlet with cases. The bivariate analysis uses Chi-Square and Fisher-Exact test, while multivariate analysis uses logistic regression in SPSS version 20 for Windows.Results: The results showed that the proportion of male and female did not differ between groups of cases and controls (50.0%; p = 1.00). Most of the case groups were > 34 years old (51.8%) while controls were ≤ 34 years old (55.4%). There were no differences in proportions at the level of education, occupation, type of residence, length of refuge, and location of refuge in the two groups (p> 0.05). The results of multivariate analysis showed that the risk factors for malaria infection after the earthquake disaster in the district of West Lombok were primarily due to the presence of gardens in refugee camps (AOR = 11,899; 95% CI: 2,369-59,774).Conclusion: The existence of gardens in refugee camps is the most influential risk factor for malaria infection after the earthquake disaster in West Lombok Regency. Latar Belakang: Malaria adalah penyakit menular yang sering muncul pada situasi sesudah bencana seperti gempa bumi. Gempa bumi yang terjadi di Lombok ini mengakibatkan kerusakan pada rumah-rumah warga dan mengharuskan mereka tinggal di tenda-tenda darurat. Kondisi ini meningkatkan risiko penularan malaria sehingga terjadi Kejadian Luar Biasa (KLB) malaria. Penelitian ini bertujuan untuk mengetahui faktor risiko infeksi malaria sesudah bencana gempa bumi di Kabupaten Lombok Barat.Metode: Penelitian kasus-kontrol (case-control) dan berlokasi di empat wilayah kerja puskesmas di Kabupaten Lombok Barat sebanyak 168 responden dilakukan pada penelitian ini. Kasus adalah penderita malaria positif berdasarkan hasil pemeriksaan mikroskopis dan tercatat dalam buku register puskesmas pada periode September-November 2018. Sedangkan kontrol adalah orang yang tidak menderita malaria berdasarkan hasil pemeriksaan mikroskopis dan tinggal di wilayah tempat penelitian. Sampel kasus dipilih secara systematic random sampling dari buku register puskesmas dan sampel kontrol dipilih secara convenient sampling dari masyarakat yang tinggal satu dusun dengan kasus. Analisis bivariat menggunakan chi square dan fisher sedangkan analisis multivariat menggunakan regresi logistik pada SPSS versi 20 untuk Windows.Hasil: Hasil penelitian menunjukkan bahwa proporsi jenis kelamin laki-laki dan perempuan tidak berbeda antar kelompok kasus dan kontrol (50,0%; p=1,00). Sebagian besar kelompok kasus berusia > 34 tahun (51,8%) sedangkan kontrol berusia ≤ 34 tahun (55,4%). Tidak terdapat perbedaan proporsi pada tingkat pendidikan, pekerjaan, jenis tempat tinggal, lama mengungsi, dan lokasi mengungsi pada kedua kelompok (p>0,05). Hasil analisis multivariat menunjukkan bahwa faktor risiko infeksi malaria sesudah bencana gempa bumi di kabupaten Lombok Barat adalah sebagian besar oleh keberadaan kebun di lokasi pengungsian (AOR=11,899; 95%IK: 2,369-59,774).Simpulan: Keberadaan kebun di lokasi pengungsian merupakan faktor risiko yang paling berpengaruh terhadap infeksi malaria sesudah bencana gempa bumi di Kabupaten Lombok barat.
Planned Behavior Theory Approach to Waste Management Behavior in South Denpasar District Daniel Beltsazar Jacob; Ni Made Utami Dwipayanti
Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education Vol. 10 No. 2 (2022): Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Educatio
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jpk.V10.I2.2022.118-129

Abstract

Background: In Bali, waste generation is estimated at 10,266.4 m3 per day. Although promotion and public education about waste management have been carried out for a long time, community behavior related to waste management is still lacking. Objective: This study aims to better understand the factors influencing waste management behavior. Thus, the promotion strategy given to the community can be ideal. Methods: A cross-sectional study was conducted in 5 sub-districts in South Denpasar District. The accessible population was all households in South Denpasar District, with a total of 36,722 households. After calculation according to the formula, the sample size was 100 and was added 10% to make 110 samples to minimize invalid data or unwilling households. As for the exclusion criteria for respondents, they were those who have lived <6 months in the sub-district of South Denpasar. The sampling technique used was Probability Proportional to Size, then analyzed using the logistic regression. The questionnaire was used to help the data collection processes. Results: People with good behavior in managing waste amounted to 55.45%. The multivariate analysis results showed a significant relationship between knowledge (AOR=2.52; 95%CI= 1.08-5.85), attitude (AOR=3.06; 95%CI= 1.13-8.28), and perceived behavior control (AOR=3.00; 95%CI= 1.22-7.38) with waste management behavior in the community. Conclusion: Efforts are needed to increase knowledge through training and education programs managed by the government and local non-governmental organizations. In addition to more frequent programs to increase community participation, accompanied by more adequate facilities and infrastructure such as carrier services and waste banks.
Defisiensi mikronutrien pada anak usia 12-59 bulan di Desa Lebih, Kabupaten Gianyar, Bali Ni Ketut Sutiari; Ni Made Utami Dwipayanti; Putu Ayu Swandewi Astuti; Kadek Nuansa Putri Wulandari; Widya Astuti
Jurnal Gizi Klinik Indonesia Vol 19, No 2 (2022): Oktober
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.76336

Abstract

Deficiency of micronutrients in children of age 12-59 months in Lebih Village, Gianyar District, BaliBackground: Lack of food intake, either in quantity or quality, will continuously cause children to get sick easily, such as being susceptible to infectious diseases and ultimately inhibiting the children’s growth.Objective: This study aimed to describe the status of micronutrients (zinc and iodine) and anemia status among children aged 12-59 months in Lebih Village. Methods: This analytic observational study used a cross-sectional design conducted in Lebih Village, Gianyar Regency. The study population was all children under five aged 12-59 months. In addition, 91 children under five were selected as research subjects using the probability proportional to size method. The data collected were the identity of the subjects (children under five and their mothers) using the interview method, serum zinc levels, urinary iodine excretion (EIU) levels, and Hb levels. Results: The results showed that the subjects’ mean age was 37.0±13.3 months, and most subjects (54.9%) were male. The biochemical examination showed that the mean serum zinc levels, the median urine iodine levels, and the mean Hb levels were 72.5±6.3 g/dL, 78.5 g/L, and 12.7±1.8 g/dL, respectively. 17.6% of the subjects had anemia, 14.3% had zinc deficiency, and 60.4% had iodine deficiency. Conclusion: Children under five aged 12-59 months in Lebih Village had micronutrient deficiencies such as zinc, iodine, and iron.