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Journal : Cropsaver : Journal of Plant Protection

Potency of Yeasts Isolated from Shallot Rhizosphere to Control Basal Rot (Fusarium oxysporum f.sp. cepae) Disease on Shallot Sri Hartati; Risma Yuniah Nur’haqi; Wahyu Daradjat Natawigena; Tarkus Suganda
CROPSAVER Vol 5, No 1 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i1.38099

Abstract

One of the major diseases of shallots is basal rot disease caused by Fusarium oxysporum f.sp. cepae (FOC). Biocontrol agents can be used as an environmentally friendly control method. Some yeasts isolated from the rhizosphere may have the potencies to control soilborne plant pathogen such as FOC. This study was objected to obtain yeast isolates from the rhizosphere of shallots that have the potencies to control basal rot. The first step was isolation of yeasts from the rhizosphere of shallots and isolation of FOC. The yeast isolates were then tested for their potencies in suppressing FOC in vitro and decreasing the basal rot disease on the shallot plants. There were ten yeast isolates obtained from the rhizosphere of shallot, in which seven isolates were non pathogenic to shallot plant. These seven isolates were further tested  for their potencies in controlling FOC and basal rot disease. The results showed that those isolates were able to inhibit the colony growth of FOC by 16,11% - 38,33% in the in vitro dual culture test, and 21,11% - 38,89% in the production of volatile compound test. The isolates also suppressed the basal rot disease incidences by 8,30% - 24,98%. Isolates Cm2 and Cm3 caused the highest disease suppression (24,98%).
Effectiveness of Several Concentrations of Entomopathogenic Nematode (Steinernema spp.) On Mortality of Spodoptera exigua Hub. in Onions Sunarto, Toto; Natawigena, Wahyu Daradjat; Irwan, Aep Wawan; Tyas, Widya Wening
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.46681

Abstract

Spodoptera exigua is one of the main pests that attack onion. An alternative that can be done to control onion caterpillars is by using the entomopathogenic nematode (Steinernema spp.) that environmentally friendly. This research aimed to obtain the concentration of the entomopathogenic nematode Steinernema spp. that is most effective in causing mortality of S. exigua in onion plants. The experiment was carried out from January to August 2020 at Citeureup, North Cimahi, Cimahi City West Java. The method used in this research was an experimental method with a randomized block design, consisted of six treatments and four replications.  The treatments (number of infective juvenile -IJ) were 0 IJ ml-1 (control), 1000 IJ ml-1, 2000 IJ ml-1, 3000 IJ ml-1, 4000 IJ ml-1, and 5000 IJ ml-1. The results showed that Steinernema spp. application at a concentration of 2000 IJ ml-1 was the most effective concentrations in causing S. exigua mortality in shallot plants (85.0 ± 5.0 %) at 72 hours after application. However, there was a tendency for treatment above a density of 2000 IJ ml-1 to decrease the mortality of S. frugiperda. Therefore, it is very necessary to consider the optimum population of nematoentomopathogens to produce high mortality of target insect pests.