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Journal : Makara Journal of Science

Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri
Makara Journal of Science Vol. 17, No. 1
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Abstract

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mxgene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.
KORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG Elfidasari, Dewi
Makara Journal of Science Vol. 12, No. 2
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Abstract

Correlation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten's Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method's. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann's correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species herons
PENGARUH PERBEDAAN LOKASI MENCARI MAKAN TERHADAP KERAGAMAN MANGSA TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA SERANG: Casmerodius albus, Egretta garzetta, Bubulcus ibis Elfidasari, Dewi
Makara Journal of Science Vol. 9, No. 1
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Abstract

Prey diversity influenced by the different or foraging areas for three species herons in Cagar Alam Pulau Dua Serang. This research is to know the influenced of foraging areas to prey diversity for three species herons in Cagar Alam Pulau Dua Serang, Banten Province. This research have been done at feeding area for the herons around Cagar Alam Pulau Dua. The data were from film shooting use Focal Observation Methods, while the film shooting analyze use scan sampling methods. It’s explain that Casmerodius albus and Egretta garzetta have preferency to select the mudflat and fisheries around CAPD as foranging location because they preferency to select fish as they main prey. Bubulcus ibis who select to using rice-field and grassland as a foraging location have preferency to select insects as a main prey. The selection of foraging location for three species herons are depend on the spesific kind and size of preys for them. The preferency for Casmerodius albus to feedinginest is mudflat with the mayority prey are big fish, with the size of more than 15 cm. Egretta garzetta have preferency to select fisheries because they main prey are fish with size of about 3-5 cm. Bubulcus ibis is prefer to feeding in grassland because they eat the insect with the preferences prey size smaller than 3 cm.
SEROSURVEILLANCE OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 WITH HAEMAGGLUTINATION-INHIBITION ON WILD AQUATIC BIRDS IN PULAU DUA SERANG NATURAL RESERVES, BANTEN PROVINCE Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri; Noor, Yus Rusila
Makara Journal of Science Vol. 15, No. 2
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Abstract

Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.
KORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG Elfidasari, Dewi
Makara Journal of Science Vol. 12, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Correlation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten's Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method's. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann's correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species herons
Co-Authors Afina Putri Shabira Afini, Irsyah Agridzadana Frisa Agrydzadana Frisa Aishah Feroza Alifandi Rahmatul Ilmi Amalia, Sheren Nur Analekta Tiara Perdana Analekta Tiara Perdana Angelia Yulita Anita Mirasaraswati Annisa Sabrina Sekar Langit Anwar Mujadin Arief Pambudi Asiah Asiah Bimo Saputro Chatarina Umbul Wahyuni Claudya Larisha Donny Gunaryadi Donny Gunaryadi Ekaristi Pratiwi Ekowati Chasanah Etyn Yunita Farah Carolina Puspaningtias Farida Ariani Fatihah Dinul Qoyyimah Fatihah Dinul Qoyyimah Fatkhurokhim Fatkhurokhim Fitriani Nurhasanah Frenzysca Yuliani Gayatri Atmadi Gintung Patantis Handhini Dwi Putri Handhini Dwi Putri Handhini Dwi Putri Haninah Haninah Haninah Haninah Haninah Haninah, Haninah Hidayat Yorianta Sasaerila Imam Rosadi Imam Rosadi Imsar Gunawan Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Isep Herdiana Iskandar, Ratih Ratna Ismi, Laksmi Nurul Kun Mardiwati Rahayu Laksmi Nurul Ismi Laksmi Nurul Ismi Lusi Anindia Rahmawati M. Yogi Riyantama Isjoni Melta Rini Fahmi Melta Rini Fahmi Melta Rini Fahmi Muhammad Qeis Tsal Sabil Mukh Syaifudin Mukh Syaifudin Mukti Ageng Wicaksono Musthofa, Siti Zuhriyah Nabilah, Dina Nadhilah Sabila Ghaisani Nanda Vindi Aini Ninditasya Wulandari Nissa Kamila Nita Noriko Nurul Robbi Sepang Pairah, Pairah Putri, Handhini Dwi Rahmadara, Gemilang Rena Riana Anita Resti Aulunia Retno Damayanti Soejoedono Riris Lindiawati Puspitasari Senjaya Mercusiana Shabira, Afina Putri Siti Fauziah Canadianti Siti Zuhriyyah Musthofa sri murtini . Syafitri Jumianto Syafitri Jumianto TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Toufan Gifari Tutik Kadarini Tutik Kadarini Widhi Wijayanti Yunus Effendi Yus Rusila Noor Yus Rusila Noor Zakia Ulfa Zakia Umami