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ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando; Roza Elvyra; Dewi Indriyani Roslim
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

JOM FMIPA Volume 1 No. 2 Oktober 2014 224 ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando, Roza Elvyra, Dewi Indriyani RoslimMahasiswa Program S1 Biologi Dosen Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Kampus Bina Widya Pekanbaru, 28293, Indonesia rizkasuciakmando@yahoo.co.idABSTRACT Scientific information of mtDNA D-loop region in fish, Kryptopterus limpok, from Riau is unknown. Before having genetic information, total DNA isolation and amplification is necessary to be carried out. This study was aimed to obtain the total DNA molecules and DNA fragment of the mtDNA D-loop region in fish K. limpok from Sungai Kampar Kiri, Riau. Ten muscle samples were taken from fish collected from the river and used for total DNA isolation. Products of DNA isolation were amplified using the PCR technique with universal primers i.e. L15926 (F) 5’ TCA AAG CTT ACA CCA GTC TTG TAA ACC 3’ dan H00651 (R) 5’ TAA CTG CAG AAG GCT AGG ACC AAA CCT 3’. The PCR process consisted of pre-PCR at 940C for 5 minutes, 35 cycles of PCR consisted of three stages, i.e. as denaturation at 940C for 30 seconds, annealing at 590C for 1 minutes, elongation at 720C for 1 minutes, and post-PCR at 720C for 5 minutes. This study obtained the intact total DNA molecules, with the total DNA concentration of 100 μg/ μl. The length of mtDNA D-loop fragment was 153 bp.
Analisis Isi Lambung Ikan Senangin (Eleutheronema tetradactylum Shaw) di Perairan Dumai Titrawani Titrawani; Roza Elvyra; Ririk Ulfitri Sawalia
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.536 KB) | DOI: 10.15408/al-kauniyah.v6i2.2823

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The research about stomach content analysis of thraedfin fish (Eleutheronema tetradactylum Shaw) “senangin fish” in Dumai aquatic was taken out on July until Maret 2011. The research aimed to know the kind of senangin fish’s food include main food, supplement food and additional food. The used analysis based on Natarjan and Jingran in Effendi (1979), by analysis index of preponderance (Higher Part Index). Based on them main food of senangin fish are  Crustacea,  Engraulidae and animal debric.
Cytochrome C oxydase 1 gene sequences long-barbel sheatfish, Kryptopterus limpok (Bleeker, 1852) from Kampar River and Indragiri River of Riau Province Roza Elvyra; Dedy Duryadi Solihin
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.59

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This study aimed to analyze the sequence of mitochondrial DNA cytochrome c oxydase 1 (Cox-1) gene of the long barbel sheatfish, Kryptopterus limpok. The Cox-1 gene of Kryptopterus limpok from Kampar and Indragiri Rivers, Riau Province was amplified using PCR technique. The length of the Cox-1 gene sequence was 655 bp containing nine nucleotide sites substituted. Four base substitutions were transition and five base was transversion. Nucleotide frequencies of guanine was a few (G:17.2%) and approximately equal in frequencies of cytosine, thymine, adenine (C: 29.5%, T: 27.5%, A: 25.8%), and G+C content (46.7%). The genetic distance of mtDNA Cox-1 gene sequences between Kryptopterus limpok from Kampar and Indragiri river was 0.01. Phylogenetic tree showed that Kryptopterus limpok from Kampar and Indragiri Rivers are in one cluster with 100% bootstrap value. Abstrak Penelitian ini bertujuan untuk menganalisis runutan gen cytochrome c oxydase 1 (Cox-1) DNA mitokondria ikan lais janggut, Kryptopterus limpok. Gen Cox-1 Kryptopterus limpok dari Sungai Kampar dan Indragiri, Provinsi Riau telah diamplifikasi menggunakan teknik PCR. Runutan gen Cox-1 yang diperoleh sepanjang 655 bp, dengan sembilan situs substitusi nukleotida, meliputi empat substitusi transisi dan lima substitusi transversi basa. Frekuensi nukleotida yang sedikit ditemukan pada guanin (G:17,2%), sedangkan frekuensi cytosin, thymin, adenin kurang lebih sepadan (C: 29,5%, T: 27,5%, A: 25,8%), dan kandungan G+C (46,7%). Jarak genetik runutan gen Cox-1 antara Kryptopterus limpok dari Sungai Kampar dan Sungai Indragiri adalah 0,01. Pohon filogeni memperlihatkan bahwa Kryptopterus limpok dari Sungai Kampar dengan Sungai Indragiri membentuk satu kelompok dengan nilai bootstrap 100%.
Seminar Nasional Pemberdayaan Masyarakat 2019 Roza Elvyra
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.i

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Puji dan syukur kami haturkan kehadirat Allah SWT, karena atas rahmat dan hidayah-Nya, Prosiding ini dapat diselesaikan dengan baik. Prosiding ini merupakan kumpulan makalah yang telah dipresentasikan dan didiskusikan dalam Seminar Nasional Pemberdayaan Masyarakat tahun 2019 yang diselenggarakan oleh Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau (LPPM Universitas Riau) pada tanggal 21 Agustus 2019 di Pekanbaru, dengan tema Dampak Nyata Pengabdian Perguruan Tinggi Membangun Negeri. Prosiding ini disusun untuk mendokumentasikan gagasan dan hasil pengabdian kepada masyarakat terkait dengan pemberdayaan masyarakat yang dikelompokkan ke dalam 6 sub tema, yaitu Ekonomi dan Sosial Budaya, Kesehatan, Komunikasi, Lingkungan Hidup, Teknologi Tepat Guna, Pendidikan. Prosiding ini diharapkan dapat memberikan wawasan tentang pemberdayaan masyarakat berkelanjutan di masa yang akan datang. Dengan demikian hasil pengabdian dalam prosiding ini dapat dijadikan sebagai rujukan dan memotivasi terwujudnya pemberdayaan masyarakat yang berkelanjutan dan membawa kesejahteraan. Prosiding ini terdiri dari hasil pengabdian dari 20 Perguruan Tinggi dan Institusi di Indonesia yaitu, Universitas Riau, STMIK Amik Riau, Politeknik Caltex Riau, Sekolah Tinggi Teknologi Dumai, Universitas Samudera Meurandeh Langsa Aceh, Universitas Sumatera Utara, Universitas Muslim Nusantara Al Washliyah Medan, Universitas Negeri Padang, Universitas Andalas, STKIP PGRI Sumatera Barat, Politeknik Pertanian Negeri Payakumbuh, Universitas Jambi, Mangrove Research Institue, Balai Penelitian dan Pengembangan Teknologi Serat Tanaman Hutan, Universitas Hasanuddin, Universitas Muhammadiyah Makassar, dan Universitas Negeri Manado. Terlaksananya kegiatan ini tidak terlepas dari dukungan berbagai pihak, untuk itu dengan segala kerendahan hati, kami atas nama panitia mengucapkan terimakasih dan penghargaan yang setinggi-tingginya kepada: Rektor Universitas Riau, Prof. Dr. Ir. Aras Mulyadi, DEA. Ketua LPPM Universitas Riau, Prof. Dr. Almasdi Syahza, SE, MP yang telah mengarahkan kegiatan ini sehingga terlaksana dengan baik. Seluruh pembicara kunci/keynote speaker, Ir. Conrad Hendrarto, MSc (Staf Ahli Menteri Bidang Pengembangan Wilayah, Kementerian Desa, Pembangunan Daerah Tertinggal dan Transmigrasi Republik Indonesia); Prof. Dr. Ocky Karna Radjasa, M.Sc (Direktur Riset dan Pengabdian Masyarakat, Kementerian Riset, Teknologi dan Pendidikan Tinggi Republik Indonesia); Prof. Dr. Almasdi Syahza, SE, MP (Ketua LPPM Universitas Riau sekaligus Ketua Forum Kuliah Kerja Nyata Badan Kerjasama Perguruan Tinggi Negeri Wilayah Barat). Bapak/Ibu peserta seminar nasional yang telah menyumbangkan artikel hasil pengabdian dalam kegiatan seminar nasional ini. Kami menyadari masih terdapat kekurangan dan kelemahan pada prosiding ini, untuk itu kritik dan saran yang bersifat membangun akan kami terima dengan senang hati. Akhir kata kami berharap prosiding ini bermanfaat bagi seluruh pihak yang terkait. Pekanbaru, September 2019 Ketua Panitia, Dr. Roza Elvyra, M.Si
Growth Pattern, Sex Ratio and Distribution of Humpback Fish (Hemisilurus heterorhynchus, Bleeker 1854) in the Flooded River Basin, Kampar Kiri, Riau Province Roza Elvyra; Febrian Lailatul Fitri; Yusfiati Yusfiati; Khairijon Khairijon; Imelda Wardani
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5176

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Flood plain river is one of the characteristics of Riau Province. The difference between H. heterorhynchus fish and other species is its distinctive mouth and bent body. H. heterorhynchus is a fish with high economic value. These fish are often overfished, which can lead to a decline in the fish population in the future. Therefore, this research was carried out to be able to see the growth pattern, sex ratio and distribution of fish so that it can become the basis for information, cultivation and preservation of H. heterorhynchus. The number caught was 78 (56.67%) male fish and 36 (43.33%) female fish. The total body length of male fish is 19-41 and female fish is 19-50 cm, the total body weight of male fish is 40-310 g and female fish is 40-600 g. The allometric growth pattern of male and female fish was negative, with b values ​​of 2.8 (males) and 2.2 (females). Correlation analysis of H. heterorhynchus fish are r= 0.98 (male) and r=0.877 (female). The sex ratio of male and female fish is 1.16:1. Male fish are 19-22 cm and 23-26 cm (small fish), 27-30 cm and 31-34 cm (medium fish), and 35-38 cm and 39-42 cm (large fish). Female fish are 19-24 cm and 25-30 cm (small fish, 31-36 cm and 37-42 cm (medium fish), 43-48 cm and 49-54 cm (large fish).
The DNA Barcode of cytb on Selais Bungkuk Fish (Hemisilurus heterorhynchus Bleeker) Originating from Riau, Indonesia Roza Elvyra
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5233

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Selais bungkuk (Hemisilurus heterorhynchus Bleeker) is one of catfishes which is a member of family Siluridae and order Siluriformes. Selais bungkuk is an endemic fish in Riau Province. The scientific study of this fish is still limited including DNA barcoding study. This research aims to analyze the DNA barcode of cytochrome b (cytb) on selais bungkuk. Methods included colleting the fishes from Kampar River (three individuals) and Tapung River (three individuals); total DNA extraction from the muscle; electrophoresis, PCR, sequencing; and bioinformatics analysis. In this study, cytb DNA sequences were obtained from the six individual fishes studied with a size of 339 bp. The six sequences have been registered at GenBank with registration numbers MH633750, MH633751, MH633752 from Kampar River, MH633753, MH633754, and MH633755 from Tapung River. The BLASTn analysis showed that the selais bungkuk fish had the highest similarity of 92.63% with H. mekongensis and the lowest (90.00%) with selais janggut (Kryptopterus limpok). There were three nucleotides that characterize the selais bungkuk fish based on the cytb sequence such as nucleotide number 205, 229, and 280. The cytb sequence can distinguish groups of catfish separately based on their species. Conclusions and recommendations, the cytb sequences obtained in this study are the first to be reported in selais bungkuk fish and can be used as a reference for identifying this species.
Karakteristik Morfometrik Hampala macrolepidota Kuhl & Van Hasselt, 1823 di Indonesia Feby Dwi Restuningsih; Roza Elvyra; Haryono Haryono
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.48688

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Hampala macrolepidota is a native fish in Indonesia that has potential as an ornamental fish and a consumption fish. The research aims were to determine the geographical classifying of fish and to analyze morphometric characteristics of H.macrolepidota morphology. The research was conducted at Fish Biosystematics Laboratory, Biosystematics and Evolution Research Center-BRIN Cibinong. Sample measured were 371 wet-preserved specimens of H. macrolepidota from various water lotic and lentic habitats in Indonesia using standard morphometric and truss morphometric methods. The collected data was analyzed using significance test with Wilk’s Lambda. Research result showed there is a tendency standard morphometrics is more accurate than truss morphometrics for classified H.macrolepidota according to their distribution area in Indonesia. This shows that H. macrolepidota on the islands of Sumatera, Jawa, and Kalimantan have a high similarity is due to the fact that the three islands are part of the Sunda Shelf.
Tingkat Kematangan Gonad dan Fekunditas Ikan Ingir-ingir (Mystus nigriceps, Valencieenes 1840) di Sungai Kampar Kiri Desa Mentulik Provinsi Riau Syifa Ainun Walidaini; Roza Elvyra
JURNAL BIOS LOGOS Vol. 12 No. 1 (2022): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v12i1.37301

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Mystus nigriceps fish in the Kampar Kiri River mentulik village is a fish consumption community and economical value so often fishing that causes a decline in the population of this fish in nature. The study aimed to examine the maturity level of gonads and the fecundity of M. nigriceps fish. Fish samples are taken once a month from February-July 2021. The parameters measured include gonad maturity level (TKG), gonad maturity index (IKG), fecundity and egg diameter. The results showed that the gonad maturity levels of male and female fish were found at the TKG I-IV stage which was dominated by the most common female fish in July. The gonad maturity index of female M. nigriceps fish is greater than that of male fish. The fecundity of M. nigriceps ranges from 626-16,370 eggs with diameters ranging from 0.43-0.75 mm and has a spawning pattern that is a total spawner.Keywords: Mystus nigriceps; gonad maturity level; gonad maturity index; fecundity; egg diameter ABSTRAKIkan Mystus nigriceps di Sungai Kampar Kiri Desa Mentulik merupakan ikan konsumsi masyarakat dan bernilai ekonomis sehingga sering dilakukan penangkapan yang menyebabkan penurunan populasi ikan ini di alam. Penelitian ini bertujuan untuk mengkaji tingkat kematangan gonad dan fekunditas ikan M. nigriceps. Sampel ikan diambil satu kali sebulan dari bulan Februari-Juli 2021. Parameter yang diukur meliputi tingkat kematangan gonad (TKG), indeks kematangan gonad (IKG), fekunditas dan diameter telur. Hasil penelitian menunjukkan bahwa tingkat kematangan gonad ikan jantan dan betina ditemukan pada tahap TKG I-IV yang didominasi oleh ikan betina paling banyak dijumpai pada bulan Juli. Indeks kematangan gonad ikan M. nigriceps betina lebih besar dibandingkan ikan jantan. Fekunditas ikan M. nigriceps berkisar antara 626-16.370 butir telur dengan diameter berkisar antara 0,43-0,75 mm dan memiliki pola pemijahan yang bersifat total spawner.Kata Kunci:  Mystus nigriceps; tingkat kematangan gonad; indeks kematangan gonad; fekunditas; diameter telur
Food Composition in The Stomach of Selais Bungkuk (Hemisilurus heterorhynchus) in The Kampar Kiri River, Mentulik Village, Riau Province Roza Elvyra; Nurul Hakiki; Yusfiati Yusfiati; Khairijon Khairijon; Imelda Wardani
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5638

Abstract

Food Composition in The Stomach of Selais Bungkuk (Hemisilurus heterorhynchus) in The Kampar Kiri River, Mentulik Village, Riau Province
Molecular Characterization of Danau Selais Fish (Ompok hypophthalmus) and Kaporeh Selais Fish (Ompok eugeneiatus) from Riau Province of Indonesia using Mitochondrial 12S rRNA Roza Elvyra; Rio Riduan; Dewi Indriyani Roslim
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.5709

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The floodplain river in Riau is inhabited by various types of vertebrate animals including fish from the genus Ompok. The rapid development of species in the Ompok genus can cause ambiguity in species identity due to inaccurate identification processes. This research aims to analyze the molecular characteristics of two types of selais fish, namely danau selais fish (O. hypophthalmus) and kaporeh selais fish (O. eugeneiatus) from Riau based on 12S rRNA sequences. Methods included sampling of fishes from the Kampar, Tapung and Indragiri Rivers, the total DNA isolation, PCR, electrophoresis, sequencing and data analysis. In this study, the 12S rRNA DNA sequence of the selais fish studied was 939 bp in size. The sequences consisted of 803 bp of conserved sites (86.15%), 127 bp of variable sites (13.52%), 53 bp of parsimony sites (5.64%) and 73 bp of singleton sites (18.57%), with the composition of Adenine 31.7 %, Cytosine 26.9%, Thymine 20.9% and Guanine 20.4%. The average genetic distance between the two species was ±0.034, with the lowest genetic distance identified in the species O. hypophthalmus and O. eugeneiatus from the Indragiri River with a value of ±0.032, while the farthest genetic distance was identified in the species O. eugeneiatus from the Kampar River and the species C. gariepinus (Out group) with a value of ±0.092. Transition substitution (19.50%) occurs more frequently than transversion (10.94%). Phylogenetic reconstruction showed that the two species can be separated into distinct groups based on 12S rRNA sequences.