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PHENYLALANINE-INDUCED MODULATION OF CALLUS CHARACTERISTICS AND SECONDARY METABOLITE ACCUMULATION IN Ocimum basilicum L. UNDER IN VITRO CONDITIONS Suminar, Erni; Mubarok, Syariful; Budiarto, Rahmat; Yulianto, Fiky; Nuraini, Anne; Yuniarti, Anni; Kusumadewi, Vira; Meliansyah, Rika; Kurnia, Dikdik; Julaeha, Siti
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.11044

Abstract

Basil (Ocimum basilicum L.) is widely used in traditional medicine due to its rich content of phenolic and flavonoid compounds. However, the natural production of these metabolites is limited. Callus culture offers a controlled method to enhance their accumulation, with medium composition, particularly precursor supplementation, playing a critical role. Phenylalanine, an aromatic amino acid, is a key precursor in the biosynthesis of phenolics and flavonoids via the shikimate pathway. This study aimed to evaluate the effect of phenylalanine on callus growth and the accumulation of total phenolic and flavonoid compounds in basil. The experiment was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, using Murashige and Skoog (MS) medium with phenylalanine concentrations of 0, 1.3, 1.6, and 2 g.L-1, each replicated six times. Results showed that phenylalanine treatments caused brown coloration, compact callus texture, and inhibited growth, as indicated by reduced fresh and dry weights. The 1.6 g.L-1 treatment produced the highest total phenolic content, while the 2 g.L-1 treatment yielded the highest flavonoid content. These findings suggest that phenylalanine can enhance secondary metabolite accumulation in basil callus, although it may suppress biomass growth.
Pemberian Kascing, Glomus sp., dan Agrobacterium pusense untuk Meningkatkan Kolonisasi Akar, Serapan P, dan Pertumbuhan Kedelai Hitam (Glycine max (L.) Merrill) pada Inceptisols Jatinangor Nurbaity, Anne; Dristiarini, Raia Zaviera; Yuniarti, Anni
Soilrens Vol 23, No 1 (2025)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v23i1.66101

Abstract

Low soil fertility was a major factor limiting national black soybean productivity. Black soybean, a functional food crop rich in isoflavones, requires effective fertilization to enhance growth.Environmentally friendly alternatives such as vermicompost and biofertilizers offer sustainableoptions. This study aimed to evaluate the effects of vermicompost and biofertilizers on rootcolonization by arbuscular mycorrhizal fungi (AMF), phosphorus (P) uptake, and black soybeangrowth, and to identify the most effective treatment. The experiment was conducted from December2024 to February 2025 in a plastic house at Bale Tatanen, Faculty of Agriculture, UniversitasPadjadjaran, Jatinangor, West Java. A Randomized Group Design with 12 treatment combinations andthree replications was used. Treatments consisted of 5 and 10 tonnes ha⁻¹ of vermicompost combinedwith either AMF or nitrogen-fixing bacteria (NFB), which were appllied to the planting holes. Datawere analyzed using ANOVA and the Scott-Knott test at a 5% significance level. Observed parametersincluded AMF root colonization, P uptake, plant height, number of leaves, chlorophyll index, leaf area,and dry weights of roots and shoots. The results showed that vermicompost and biofertilizersincreased AMF colonization, P uptake, plant height, leaf number, chlorophyll index, and shoot dryweight. The application of 5 tonnes ha⁻¹ vermicompost combined with AMF was the most effectivetreatment. This study provided useful recommendations for vermicompost and biofertilizerapplication to support soil fertility improvement and black soybean growth on marginal lands.
PERTUMBUHAN DAN HASIL TANAMAN JAGUNG MANIS (ZEA MAYS SACCHARATA STURT) AKIBAT PEMBERIAN DOSIS PUPUK MAJEMUK NPK Solihin, Eso; Sudirja, Rija; Yuniarti, Anni
AGRO TATANEN | Jurnal Ilmiah Pertanian Vol. 6 No. 2 (2024): AGRO TATANEN Edisi Juli 2024 | Jurnal Ilmiah Pertanian
Publisher : Program Studi Agroteknologi Faperta UNIBBA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55222/agrotatanen.v6i2.1435

Abstract

Sweet corn (Zea mays saccharata Sturt) is one of the food crops that is needed to meet food needs. Efforts to increase the quantity and quality of sweet corn can be done through fertilization. To ensure that sweet corn's nutritional requirements for nitrogen (N), phosphorus (P) and potassium (K) are met, fertilizer such as NPK can be applied. This research aims to analyze the effect of various doses of NPK fertilizer on the growth and yield of sweet corn. The research was carried out at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University, Jatinangor, Sumedang, from November 2023 to March 2024. The research used a Complete Randomized Block Design (RAK) with ten treatments and three replications. The treatments given include: Control (without N, P, K), Standard N, P, K (Urea, SP-36, and KCl), and NPK fertilizer with varying doses (¼, ½, ¾, 1, 1 ¼, 1 ½ , 1 ¾, and 2). The research results showed that the application of NPK compound fertilizer had a significant effect on growth parameters, yield components and sweet corn yield. Meanwhile, the most effective dose in increasing plant yields was H treatment (1 ½ doses of NPK).
Sosialisasi Pengelolaan Hama Tanaman Bawang Merah (Allium ascalonicum L.) Dataran Tinggi dengan Cara Menerapkan Sistem Budidaya Tanaman yang Baik di Desa Pasigaran, Kecamatan Tanjungsari, Kabupaten Sumedang, Jawa Barat Rasiska, Siska; Suminar, Erni; Nuraini, Anne; Yuniarti, Anni; Setiawan, Iwan
Agrikultura Masyarakat Tani Vol 2, No 1 (2024): November
Publisher : Fakultas Pertanian, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrimasta.v2i1.57608

Abstract

Desa Pasigaran terletak di Kecamatan Tanjungsari, Kabupaten Sumedang dengan ketinggian tempat 979 meter di atas permukaan laut. Pengunaan lahannya didominasi oleh lahan sawah, lahan kering, dan permukiman. Lahan kering dan pekarangan di permukiman berpotensi dikembangkan sebagai lahan tanaman bawang merah yang memiliki nilai kelayakan usahatani yang tinggi.Salah satu kendala produksi tanaman bawang merah adalah serangan hama, yang dapat diatasi salah satunya dengan praktik budidaya tanaman bawang yang baik. Kegiatan pengabdian ini bertujuan untuk mensosialisasikan hama utama tanaman bawang merah dan mempraktikkan cara pengendaliannya. Pendekatan yang dilakukan dalam kegiatan pengabdian ini adalah 1) diskusi berupa tukar pikiran mengenai nilai penting tanaman bawang merah dan masalah-masalah umum dalam usahatani bawang merah; 2) penyuluhan mengenai hama utama tanaman bawang merah dan cara pengendaliannya; serta 3) pelatihan mengenai praktik budidaya tanaman bawang yang baik. Kegiatan pengabdian ini melibatkan sekelompok wanita tani yang berjumlah 20 orang. Berdasarkan hasil diskusi menunjukkan para peserta belum mengetahui secara tepat nilai ekonomi, sosial, dan budaya dari tanaman bawang merah di Indonesia, namun mengetahui sebagian manfaat dari tanaman tersebut. Selain itu, para peserta tidak mengetahui hama utama tanaman bawang merah dan cara pengendaliannya . Hasil pengabdian menunjukkan bahwa sebagian besar peserta dapat  mengikuti semua kegiatan dengan baik, terutama ketika dialog, penyuluhan dan praktek secara langsung di lapangan. Kegiatan inimampu 1) meningkatkan pengetahuan mengenai praktik budidaya tanaman bawang yang baik; 2) meningkatkan kewaspadaan terhadap hama utama tanaman bawang; dan 3) meningkatkan ketrampilan dalam mengendalikan hama tanaman bawang merah.
Pengaruh Pembenah Tanah Cair dan Pupuk N, P, K terhadap Pertumbuhan Serta Hasil Tanaman Bawang Merah (Allium cepa, L.) Solihin, Eso; Sudirja, Rija; Yuniarti, Anni; Kamaluddin, Nadia Nuraniya
Jurnal Ilmiah Universitas Batanghari Jambi Vol 24, No 3 (2024): Oktober
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jiubj.v24i3.5268

Abstract

This research aims to determine the effects of humic acid application on soil pH, organic carbon (C-Organic), cation exchange capacity (CEC), as well as the growth and yield of shallots. The study was conducted at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University, Jatinangor, Sumedang, from November 2023 to February 2024. The experimental method used was a Randomized Complete Block Design (RCBD) with six treatments and five replications. The treatments were: A; (0.5 Soil Amendment and 1 Standard N, P, K), B; (1 Soil Amendment and 1 Standard N, P, K), C; (1.5 Soil Amendment and 1 Standard N, P, K), E; Without Standard N, P, K and Soil Amendment (Control), and F; (Standard N, P, K). The results showed that the combination of soil amendments and N, P, and K fertilizers significantly affected the parameters of soil pH, C-Organic, CEC, and plant height at 14, 28, 42, and 56 days after planting (DAP); stem diameter at 14, 28, 42, and 56 DAP; number of tillers at 14, 28, 42, and 56 DAP; and shallot plant weight.