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Journal : Journal of Fisheries

The Optimization of Sulphide Oxidizing Bacteria (SOB) for Oil Corrosivity Reduction at Indramayu Coast, The Northern Coastal Area of West Java Yudi Nurul Ihsan; Kalysta Fellatami; Rega Permana; Jiang Mingguo; Tri Dewi Kusumaningrum Pribadi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.33462

Abstract

Highlight Research First NR-SOB from Indramayu coast was successfully isolated and identified. Bactrerial characterization morphologically and biochemically, foster with in situ hybridization confirm the isolate was Thiobacillus denitrificans, and Arcobacter sp. Both isolate, Thiobacillus denitrificans and Arcobacter sp., were able to grow in sulphide rich environment in the presence of essential nutrient Thiobacillus denitrificans and Arcobacter sp. exhibit an outstanding sulphide oxidation ability up to 100% in the presence of nitrate.   Abstract Crude oil production triggers the formation of hydrogen sulphide, also known as souring, which is extremely toxic and corrosive to the environment. It additionally give an adverse consequence to aquatic, terrestrial, and human existence. Studies of hydrogen sulphide reduction in sediments polluted by crude oil have been carried out recently to investigate the capability of indigenous Nitrate-Reducing Sulphide Oxidising Bacteria, hereinafter referred to as NR-SOB, as bioremediation agents. The experiments utilised hydrogen sulphide with 200 µM concentration combined with NO3 with different concentrations of 100 µM, 200 µM, and 300 µM. Measurements of the hydrogen sulfide concentrations were observed up to 48 hours within the experimental period. The SOB used in this study were taken from Balongan Bay at Indramayu coast using Nansen bottle to carry out water sample. The sulphide-oxidising ability of SOB was then evaluated at room temperature in control environment. Methylene blue method was applied to monitor the sulphide concentration. The results showed a complete removal of hydrogen sulphide concentrations in 48 hours accompanied with gradual drops of nitrate in all experiment series. Sulphide oxidation rate was detected to appear between 6.8 and 10.2 fmol/cell/hour. Measurements of cell abundance after 48 hours showed 6.2 x 105, 7.5 X 105, and 8.2 X 105 cell/ml from Experiments I, II, and III respectively. Using MSS selective medium, the bacteria were identified as Thiobacillus denitrificans and Arcobacter sp. Overall, the isolated NR-SOB from the coast of Balongan Bay, Indramayu proves to be a promising candidate for sulphide controls and mitigation.
Co-Authors Archie S. Eka Buana Aris Nuryana Atikah Nurhayati Ayip Choerul Rizal Budhi Gunawan Darmawan, Rivaldi Thio Deni Ramdani Dhahiyat, Ahmad Prawira Dita Agustian Donny Juliandri Prihadi Eddy Afrianto Eddy Afrianto Erri Noviar Megantara Evi Liviawaty Evi Nurul Ihsan Faisal Rizki Hadi Fellatami, Kalysta Frederika, Yanthi Christin Hasna, Azalea Fathin Herman Hamdani Herman, Adam Herry Yogaswara Ihsan, Evi Nurul Iklima Hasanah Indah Riyantini Indah Riyantini Indah Riyantini Ine Maulina Jiang Mingguo Junirahma, Nur Sakinah Kalysta Fellatami Kalysta Fellatami Kalysta Fellatami Kalysta Fellatami Kalysta Fellatami Keukeu Kaniawati Rosada Lintang P. S. Yuliadi Lintang Permata Sari Juliadi Lintang Permata Sari Yuliandi Lintang Permatasari Yuliadi Luthfiyyah Azizah M. Rudyansyah Ismail Martha Fani Cahyandito Meitha Permata Sari MS, Yuniarti Muflihah, Fahira Nuril Muhamad Akmal Rizkifar Nawang Nila Ririsanti Nenci Haryadi Nia Rossiana Nira Nirwana Nirwa, Nira na Nopa Firmansyah Nurfitriani, Anggia Nurul D. M. Sjafrie Nuryana, Aris Okliandi Saputra Phobe Nadine Alfa Zefa Raden Wandha Humaira Ramdan Nurdiana Ramdan Nurdiana, Ramdan Rega Permana Rega Permana Rizky K. Bangsa Rusky Intan Pratama Satriawan, Ratu Balqist Priesty Higina Sheila Zallesa Subiyanto Subiyanto sunarto sunarto Sunarto Sunarto Supian, Sudradjat Syamsuddin, Mega Laksmini Titin Herawati Tombus Lucas Anggara Tri Dewi K Pribadi Tri Dewi K Pribadi Tri Dewi K. Pribadi Tri Dewi K. Pribadi Tri Dewi Kusumaningrum Pribadi Tri Dewi Kusumaningrum Pribadi Triyono Triyono Ujang Subhan Vera Bararah Barid Walim Lili Yeni Mulyani Yuniarti MS Yuniarti Yuniarti Zallesa, Sheila