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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Indonesian Journal of Biotechnology Journal of General Procedural Dermatology and Venereology Indonesia IGKOJEI: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Journal of General-Procedural Dermatology & Venereology Indonesia
Yuda Heru Fibrianto
Departemen Fisiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Effect of Green Fluorescent Protein (GFP) on the Development of Canine Intergeneric Embryo= Pengaruh Green Fluorescent Protein (GFP) Pada Perkembangan Anjing Dari Embryo Intergeneris. Yuda Heru Fibrianto
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2326.859 KB) | DOI: 10.22146/jsv.356

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The present study investigated the effect of green fluorescent protein on the development of canine intergeneric clone embryo with bovine oocyte recipient. Cumulus oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 10% (v/v) fetal bovine serum (FBS) (Life Technologies), 0.005 U/m1 bovine FSH (Antrin®, Denka Kanagawa, Japan) and 1 pg/m1 estradiol (Sigma-Aldrich) at 39 °C in a humidified atmosphere of 5% CO2 in air and donor cell tranfected with enhanced green fluorescent protein. In this experiment GFP have no negative effect in fusion or embryo development but the expression rate were decreased in 2 cell stage and then the expression gradually decreased with progression of embryo development.
Kuantitas Dan Kualitas Sel Telur Anjing Lokal Dari Berbagai Stadium Estrus Yuda Heru Fibrianto; Tri Wahyu Pangestiningsih; Amelia Hanna; Pudji Astuti; ClaudeM Mona Airin; Asyhari .; Amrullah Anindito; Nuraini Rahmawati; Koko Kurniawan; Pradityo Yoga Wibowo
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1310.87 KB) | DOI: 10.22146/jsv.411

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Perbandingan Pengaruh Penambahan Cairan Folikel Sapi dengan Babi terhadap Maturasi Oosit Anjing (Canis familiarsis) secara in Vitro dari Stadium Estrus Ovarium Yuda Heru Fibrianto; Tri Wahyu Pangestiningsih; Amelia Hana; Claude Mona Airin; Nuraini Rahmawati
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1847.065 KB) | DOI: 10.22146/jsv.427

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Pemasakan Oosit Anjing (Canis familiarsis pada Stadium Anestrus dan Diestrus pada Media Maturasi yang Diperkaya dengan Cairan Folikel Sapi 10% dengan Penambahan Follicle Stimulating Hormone (FSH) dan Luteotropic Hormone (LH) Yuda Heru Fibrianto; Amelia Hana; Tri Wahyu Pangestiningsih; Pradityo Yoga Wibowo; Claude Mona Airin
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4904.308 KB) | DOI: 10.22146/jsv.430

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Lumba-Lumba Hidung Botol Laut Jawa Adalah Tursiops aduncus Berdasar Sekuen Gen NADH Dehidrogenase Subunit 6 (VERIFICATION BOTTLENOSE DOLPHINS FROM JAVA SEA IS TURSIOPS ADUNCUS BASED ON GENE SEQUENCES OF NADH DEHYDROGENASE SUBUNIT 6) Rini Widayanti; Yuda Heru Fibrianto; Woro Danur Wendo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.256 KB)

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Bottlenose dolphins (Tursiops sp.) is one of the aquatic mammals widely spread in the marines ofIndonesia archipelago, especially the Java Sea. The taxonomy of the genus Tursiops is still  controversial.The purpose of this study was to examine the molecular basis of Tursiops sp of Java sea marine origin onthe basis of its NADH dehydrogenase gene subunit 6 (ND6) sequences. Samples of blood were collectedfrom five male bottle nose dolphins from captivity of PT. Wersut Seguni Indonesia. DNA was isolated,amplified by polymerase chain reaction (PCR), sequenced, and analyzed the data using the MEGA v. 5.1program. The results of PCR amplification was 868 base pairs (bp), DNA sequencing showed that 528nucleotides were ND6 gene, nucleotide at the position of 387 could be used to distinguish the bottle nosedolphins Java marine origin with T. aduncus.   Filogram using Neighbor joining method based on thenucleotide sequence of the gene ND6, showed that bottle nose dolphins Java marine origin belong to groupof T. aduncus.
Profil Saraf Nitrergik Sekum Ayam Pedaging yang Diinfeksi Eimeria tenella (THE PROFILE OF NEORAL NITRERGIC IN THE COECUM OF BROILER INFECTED WITH Emeria tenella) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin .; Claude Mona Airin
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.728 KB)

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Neurotransmitter found in the enteric nervous system that plays an important role in a variety ofenteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to bedependent on the manner by which inflammation is caused. This study was performed to determine theprofile of neural nitrergic with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d)staining in the cecum of broiler infected by Eimeria tenella (E. tenella). Thirty six health free of coccidiosisbroiler one-day old chickens (DOC) used in this study. All of broiler adapted for 7 days with food and drinkgiven ad libitum then divided into three groups, each group consisted of 12 broilers. The 1st group wascontrol only given orally 1.0 ml of distilled water/ heads , while 2nd group was infected with a single dose of5x103 oocysts/ head orally, and 3rd group was infected with a single dose of 2x104 oocysts/ head orally. Onday 7 post infection, all of chickens were fasted for 12 hours then were euthanized and cecum was taken.Lesion score of cecum was assessed. Furthermore, tissue of the coecum was prepared for hisochemicalusing NADPH-d staining to determine morphology, and morhometric of nitrergic neurons. The resultshown that cecum lesion score of group I is 0, group II is +2, group III is +3. Neuron density in the cecum ofgroup I is 2794.96+4.65 neuron/cm2, group II is 4930.36+63.73 neuron/cm2 and group III is 7892.31+44.97 neuron/cm2. Neuron density of group II and III increased signifcantly (p<001) than group I. Nitrergic neurondesity of group III increased significantly (p<0.05) than that of group II. It was concluded that the infectionof E.tenella led to increase the number of neutrl mienteric nitrergic of the cecum. The higher lesion score ofcecum led to increase the number of mienteric nitrergic neuron.
Model Desa Binaan (Tahun II) Pembinaan Teknis Budidaya Kambing Kacang di Desa Kemiri, Tanjungsari, Gunung kidul, Yogyakarta: Assisted Village Model (Year II) Technical Development of Kacang Goat Cultivation in Kemiri Village, Tanjungsari, Gunung Kidul, Yogyakarta Sarmin Sarmin; Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Claude Mona Airin
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 2 No. 3 (2021): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v2i3.254

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Kegiatan desa binaan tahun ke-2 ini sebagai bagian dari desa binaan 3 tahun yang bertujuan menjadikan Kemiri Sebagai Kawasan Petenakan Terpadu. Tahapan tahun ni menitikberatakan pada upaya peningkatan keterampilan budi daya kambing pada masyarakat Kemiri, kecamatan Tanjungsari, Kabupaten Gunungkidul. Kegiatan dilakukan dengan inisiasi budidaya kambing kacang dan konsultatif. Pendampingan lanjutan diperlukan untuk semakin meingkatkan kapasitas peternak dan menyangga kemandirian ekonomi peternak melalui budi daya kambing berupa pemasaran, teknologi pasca produksi, pengolahan pakan dan limbah
KAJIAN MOLEKULER LUMBA-LUMBA HIDUNG BOTOL (Tursiops sp.) ASAL LAUT JAWA BERDASAR URUTAN GEN CYTOCHROME C OXIDASE SUB-UNIT II (COX II) (Molecular Studies of Bottlenose Dolphins (Tursiops sp.) from Java Sea by Gene Sequences of Cytochrome C Oxidase Sub-Unit II (COX II)) Rini Widayanti; Yuda Heru Fibrianto
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.962 KB) | DOI: 10.21157/j.ked.hewan.v10i1.3357

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The purpose of this study was to examine the molecular basis of Tursiops sp. originated from Java sea based on gene sequences ofCytochrome C Oxidase sub-unit II. Samples of blood were collected from five male bottlenose dolphins from PT. Wersut Seguni Indonesiacaptivity. Total deoxyribonucleic acid (DNA) was isolated from blood samples, then amplified by polymerase chain reaction (PCR), sequenced,and the data were analyzed using MEGA version 5.1 program. The amplification result of the PCR product obtained 824 base pairs (bp), andDNA sequencing obtained 684 nucleotides constituent of COX II genes that encode 228 amino acids making up the COX II. Nucleotide andamino acid sequence of COX II bottlenose dolphins originated from Java sea has a very high homology to Tursiops aduncus that are 99.13%and 100% respectively. Phylogram by neighbour joining method based on the nucleotide sequence of COX II gene indicating bottlenose dolphinsfrom Java sea belongs to group of Tursiops aduncus.Key words: bottlenose dolphin, COX II gene, nucleotide, Jawa Sea
KAJIAN KADAR KORTISOL SAPI YANG DIPOTONG DI RUMAH POTONG HEWAN YOGYAKARTA Sarmin S; Amelia Hana; Yuda Heru Fibrianto; C. Mona Airin
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.034 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2635

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Penelitian ini bertujuan mengkaji tingkat stres sapi yang dipotong di Rumah Potong Hewan (RPH) Yogyakarta. Dalam penelitian ini digunakan 20 ekor sapi yang dibagi menjadi dua kelompok, yakni kelompok sapi yang dilakukan restraint dengan restraining box tipe Mark 1 (K1, n=13) dan kelompok sapi yang dilakukan restraint dengan metode konvensional (K2, n= 7). Masing-masing kelompok diambil darahnya sebelum dan saat dipotong untuk diperiksa kadar kortisol dengan enzyme-linked immunosorbent assay (ELISA). Rata-rata kortisol sapi betina sebelum vs setelah restraint pada K1 dan K2 masing-masing adalah 55,64±52,13 vs 21,23±20,63 ng/ml dan 127,48±150,19 vs 106,28+75,39 ng/ml. Rata-rata kortisol sapi jantan sebelum vs setelah restraint pada K1 dan K2 masing-masing adalah 40,28±38,12 vs 24,21±17,21 ng/ml dan 49,51±38,67 vs 69,62±63,98 ng/ml. Rata-rata kadar kortisol tanpa memperhatikan jenis kelamin pada K1 dan K2 masing-masing adalah 79,50±88,50 vs 95,80±69,69 ng/ml (P>0,05). Disimpulkan bahwa tidak terdapat perbedaan kadar kortisol sebagai indikator stres pada sapi-sapi yang dipotong di RPH Yogyakarta yang mengalami restraint dengan restraining box tipe Mark 1 dan restraint metode konvensional.
The effect of injection of ADSC compared to APPE on collagen density in aging skin (animal study) Pricilla, Erlina; Julianto, Indah; Kariosentono, Harijono; Budiani, Dyah R; Fibrianto, Yuda Heru; Rindiastuti, Yuyun; Veraida, Ainun
Journal of General - Procedural Dermatology & Venereology Indonesia Vol. 2, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction: Aging is a biological process that can induces changes to the structural integrity and physiological function of skin. Structural changes of skin are a result of dermal atrophy, decreased collagen, the loss of subcutaneous fat, the loss of inherent elasticity, and increased melanogen.1,5,6 From the definition itself, a stem cell characterized by its ability to self-renew and also have an ability to differentiate along multiple lineage pathways.2,4 ADSC which is a kind of mesenchymal stem cell from human adipose tissue, have some potential properties as stem cells derived from bone marrow.4,7 Objective: The aim of this study was to verify the comparison effect of ADSCs and their secretome for skin aging treatment. Methods: ADSC were isolated from lipoaspirates obtained from healthy donors after obtaining written consent and ethical approval using liberase enzymatic digestion, washed with steril PBS and centrifuged. After the phase, ADSC were seeded directly after isolation with liberases and cultured, then produced the conditioning media (AAPE) by hypoxia amplifies.3,4,6,7 We analysed the ADSCs (2,5 x 105 cells) and their secretomes (0,1ml in 0,4ml NaCl 0,9%) by subcutaneous injection on the back of a rat (with range age 48 weeks), and followed began every two weeks after injected until six weeks and stained with Van Giesson staining, for measured the density of collagen.3,5,6 Results: Collagen density was increased after ADSC injected to the skin. From statistical analysis showed there was a significant result of collagen density compared to control group and AAPE (p<0.05). We conclude that ADSC had anti-aging potential or regenerative by stimulating collagen synthesis of dermal fibroblast. ADSC will be as new modality treatment for aging skin in future.
Co-Authors . Ariana Agustin Indrawati Agustina Dwi Wijayanti Ainun Veraida Amelia Hana Amelia Hana Amelia Hana Amelia Hana Amelia Hana Amelia Hanna Amrullah Anindito Annisa Hidayati Arinda Devi Larasati Asyhari . Betty Sundari Budiani, Dyah R C. Mona Airin C. Mona Airin, C. Claude Mona Airin ClaudeM Mona Airin Dian Bekti Hadi Masithoh Dyah R Budiani Erlina Pricilla Fitriana, Ida Guntari Titik Mulyani Harijono Kariosentono Indah Julianto Indah Julianto, Indah Irma Padeta Kariosentono, Harijono Koko Kurniawan Marla Anggita Nuraini Rahmawati Pradityo Yoga Wibowo Pricilla, Erlina PUDJI ASTUTI Pudji Astuti Puspa Wikan Sari Raden Gagak Donny Satria Rini Widayanti Rini Widayanti Rini Widayanti Sarmin . Sarmin Sarmin Sarmin Sarmin Setiawan, Dwi Cahyo Budi Surya Agus Prihatno Teguh Budipitojo Teguh Budipitojo Tri Wahyu Pangestiningsih Veraida, Ainun Widagdo Sri Nugroho Woro Danur Wendo Yosua Kristian Adi Yuyun Rindiastuti Yuyun Rindiastuti